BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20251
(746 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42970.1 68418.m05241 COP9 signalosome complex subunit 4 / CS... 78 5e-15
At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal hydrolase-r... 31 0.61
At1g58450.1 68414.m06649 peptidyl-prolyl cis-trans isomerase FKB... 30 1.4
At5g52400.1 68418.m06501 cytochrome P450 family protein similar ... 27 10.0
At3g02690.1 68416.m00260 integral membrane family protein simila... 27 10.0
>At5g42970.1 68418.m05241 COP9 signalosome complex subunit 4 / CSN
complex subunit 4 (CSN4) (COP8) (FUS4) FUSCA4, COP8,
CSN4; identical to CSN complex subunit 4 [Arabidopsis
thaliana] GI:18056659, COP8 [Arabidopsis thaliana]
GI:5802627; contains Pfam profile PF01399: PCI domain;
identical to cDNA CSN complex subunit 4 (CSN4)
GI:18056658
Length = 397
Score = 78.2 bits (184), Expect = 5e-15
Identities = 37/80 (46%), Positives = 52/80 (65%)
Frame = +2
Query: 269 FIEAIVNENVSLVISRQLLTDVSTHLALLADNVSQEVSHFALDVIQPRVISFEEQVASIR 448
FI+ I++++V LV+SRQLL + L L +E++ F L IQPRV+SFEEQ IR
Sbjct: 40 FIDHILSDDVPLVVSRQLLQSFAQELGRLEPETQKEIAQFTLTQIQPRVVSFEEQALVIR 99
Query: 449 QHLADIYERNQNWKEAANVL 508
+ LA +YE Q W +AA +L
Sbjct: 100 EKLAGLYESEQEWSKAAQML 119
Score = 75.4 bits (177), Expect = 4e-14
Identities = 37/77 (48%), Positives = 53/77 (68%), Gaps = 1/77 (1%)
Frame = +1
Query: 511 GIPLETGQKQYSVDYKLETYLKIARLYLEVDDPVQAEAFVNRASLLQAETTNEQLQIYYK 690
GI L++G + ++KL ++IARLYLE DD V AEAF+N+AS L + + NE L + YK
Sbjct: 121 GIDLDSGMRAVDDNFKLSKCIQIARLYLEDDDAVNAEAFINKASFLVSNSQNEVLNLQYK 180
Query: 691 VCYAE-FRL*KEFIEAA 738
VCYA + ++F+EAA
Sbjct: 181 VCYARILDMKRKFLEAA 197
>At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of
unknown function (DUF627)
Length = 1086
Score = 31.5 bits (68), Expect = 0.61
Identities = 19/63 (30%), Positives = 38/63 (60%)
Frame = +2
Query: 248 IIKSLKAFIEAIVNENVSLVISRQLLTDVSTHLALLADNVSQEVSHFALDVIQPRVISFE 427
++KS+ ++++V V LV S+ LL + ++ ++LL D VS V + ++QP + E
Sbjct: 623 VLKSIN-LLKSVVTYKVPLVDSKILLVE-NSRISLLKDLVSLSVFDYRSYILQPVKLYLE 680
Query: 428 EQV 436
E++
Sbjct: 681 EEL 683
>At1g58450.1 68414.m06649 peptidyl-prolyl cis-trans isomerase
FKBP-type family protein similar to rof1 from
(Arabidopsis thaliana) GI:1373396, GI:1354207; contains
Pfam profile PF00515 TPR Domain
Length = 164
Score = 30.3 bits (65), Expect = 1.4
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = +1
Query: 580 ARLYLEVDDPVQAEAFVNRASLLQAETTNEQLQIYYK 690
A+ Y+EV D + AE +NRA L+A+ N +++ YK
Sbjct: 99 AQSYIEVGDLISAEMDINRA--LEADPENREVKSLYK 133
>At5g52400.1 68418.m06501 cytochrome P450 family protein similar to
Cytochrome P450 72A1 (SP:Q05047) (cytochrome P450 lxxii
hydroxylase) (ge10h) [Catharanthus roseus]
Length = 519
Score = 27.5 bits (58), Expect = 10.0
Identities = 19/76 (25%), Positives = 35/76 (46%)
Frame = +2
Query: 203 VSQCSYGDTKKYRTRIIKSLKAFIEAIVNENVSLVISRQLLTDVSTHLALLADNVSQEVS 382
+++ S+G T + T+++K+L+A A+ N N + +S + A + E+
Sbjct: 220 IAKTSFGVTGENGTQVLKNLRAVQFALFNSN--RYVGVPFSNILSYKQTVKAKGLGHEID 277
Query: 383 HFALDVIQPRVISFEE 430
L I R IS E
Sbjct: 278 GLLLSFINKRKISLAE 293
>At3g02690.1 68416.m00260 integral membrane family protein similar
to PecM protein (GI:5852331) {Vogesella indigofera} and
PecM protein (SP:P42194) [Erwinia chrysanthemi]
Length = 417
Score = 27.5 bits (58), Expect = 10.0
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -3
Query: 360 SASSAKCVLTSVKSCLEITRLT 295
S+SS+ C S SCL ITR T
Sbjct: 12 SSSSSSCFFASPNSCLSITRRT 33
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,386,630
Number of Sequences: 28952
Number of extensions: 311959
Number of successful extensions: 809
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 761
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 809
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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