Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20234
(575 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 169 3e-43
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 168 4e-43
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 168 4e-43
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 99 5e-22
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 90 2e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 68 1e-12
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 48 7e-07
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 44 2e-05
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 36 0.004
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.006
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.006
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 32 0.052
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 31 0.12
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.21
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 30 0.28
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.37
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 1.5
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 169 bits (410), Expect = 3e-43
Identities = 77/85 (90%), Positives = 81/85 (95%)
Frame = +2
Query: 254 EMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS 433
E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTS
Sbjct: 48 ELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTS 107
Query: 434 QADCAVLIVAAGTGEFEAGISKNGQ 508
QADCAVLI+ GTGEFEAGISK+GQ
Sbjct: 108 QADCAVLIIGGGTGEFEAGISKDGQ 132
Score = 98.3 bits (234), Expect = 7e-22
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +3
Query: 114 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA 251
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA 46
Score = 43.6 bits (98), Expect = 2e-05
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +1
Query: 508 TREHALLAFTLGVKQLIVGVNK 573
TREHALLA+TLGVKQLIV VNK
Sbjct: 133 TREHALLAYTLGVKQLIVAVNK 154
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 168 bits (409), Expect = 4e-43
Identities = 76/85 (89%), Positives = 81/85 (95%)
Frame = +2
Query: 254 EMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS 433
E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTS
Sbjct: 48 ELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTS 107
Query: 434 QADCAVLIVAAGTGEFEAGISKNGQ 508
QADCA+LI+ GTGEFEAGISK+GQ
Sbjct: 108 QADCAILIIGGGTGEFEAGISKDGQ 132
Score = 98.3 bits (234), Expect = 7e-22
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +3
Query: 114 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA 251
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA 46
Score = 43.6 bits (98), Expect = 2e-05
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +1
Query: 508 TREHALLAFTLGVKQLIVGVNK 573
TREHALLA+TLGVKQLIV VNK
Sbjct: 133 TREHALLAYTLGVKQLIVAVNK 154
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 168 bits (409), Expect = 4e-43
Identities = 76/85 (89%), Positives = 81/85 (95%)
Frame = +2
Query: 254 EMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS 433
E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNMITGTS
Sbjct: 48 ELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTS 107
Query: 434 QADCAVLIVAAGTGEFEAGISKNGQ 508
QADCA+LI+ GTGEFEAGISK+GQ
Sbjct: 108 QADCAILIIGGGTGEFEAGISKDGQ 132
Score = 98.3 bits (234), Expect = 7e-22
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = +3
Query: 114 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA 251
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA 46
Score = 43.6 bits (98), Expect = 2e-05
Identities = 20/22 (90%), Positives = 21/22 (95%)
Frame = +1
Query: 508 TREHALLAFTLGVKQLIVGVNK 573
TREHALLA+TLGVKQLIV VNK
Sbjct: 133 TREHALLAYTLGVKQLIVAVNK 154
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 98.7 bits (235), Expect = 5e-22
Identities = 46/83 (55%), Positives = 55/83 (66%)
Frame = +2
Query: 260 GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 439
GKGSF YAW+LD + ER RG+T+D+A FE+ K I DAPGHRDFI MI G S A
Sbjct: 220 GKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSA 279
Query: 440 DCAVLIVAAGTGEFEAGISKNGQ 508
D AVL+V + FE G +NGQ
Sbjct: 280 DFAVLVVDSSQNNFERGFLENGQ 302
Score = 46.0 bits (104), Expect = 4e-06
Identities = 19/42 (45%), Positives = 30/42 (71%)
Frame = +3
Query: 126 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA 251
K +++VV GHVDSGKST G ++++ G I+ R+++K EA
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEA 216
Score = 30.7 bits (66), Expect = 0.16
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1
Query: 490 YL*ERSTREHALLAFTLGVKQLIVGVNK 573
+L TREHA L LG+ +++V VNK
Sbjct: 297 FLENGQTREHAYLLRALGISEIVVSVNK 324
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 90.2 bits (214), Expect = 2e-19
Identities = 39/92 (42%), Positives = 57/92 (61%)
Frame = +2
Query: 233 EVREGGPEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIK 412
++ E GK S+ +W LD ERE+G T+++ FET +++DAPGH+ ++
Sbjct: 272 KIEREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVT 331
Query: 413 NMITGTSQADCAVLIVAAGTGEFEAGISKNGQ 508
NMI G SQAD VL+++A GEFEAG + GQ
Sbjct: 332 NMINGASQADIGVLVISARRGEFEAGFERGGQ 363
Score = 61.3 bits (142), Expect = 1e-10
Identities = 26/48 (54%), Positives = 38/48 (79%)
Frame = +3
Query: 126 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQKWVKD 269
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA++ K+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKE 283
Score = 31.1 bits (67), Expect = 0.12
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +1
Query: 508 TREHALLAFTLGVKQLIVGVNK 573
TREHA+LA T G+ L+V +NK
Sbjct: 364 TREHAVLARTQGINHLVVVINK 385
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 67.7 bits (158), Expect = 1e-12
Identities = 31/75 (41%), Positives = 48/75 (64%)
Frame = +2
Query: 254 EMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS 433
++G+ SF +DK E+ RGITI A ++ET+ + +D PGH D+IKNMITG +
Sbjct: 79 DLGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAA 138
Query: 434 QADCAVLIVAAGTGE 478
D A+++V+A G+
Sbjct: 139 TMDGAIIVVSATDGQ 153
Score = 35.1 bits (77), Expect = 0.007
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +3
Query: 120 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 206
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
Score = 32.7 bits (71), Expect = 0.040
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +1
Query: 508 TREHALLAFTLGVKQLIVGVNK 573
TREH LLA +GVKQ++V +NK
Sbjct: 157 TREHLLLARQVGVKQIVVYINK 178
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 48.4 bits (110), Expect = 7e-07
Identities = 25/63 (39%), Positives = 33/63 (52%)
Frame = +2
Query: 287 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 466
V+D L AER+RGITI+ A F + +ID PGH DF + + D AV I+
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126
Query: 467 GTG 475
G
Sbjct: 127 SAG 129
Score = 28.7 bits (61), Expect = 0.64
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 138 NIVVIGHVDSGKSTTTGHLIYKCG 209
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 44.0 bits (99), Expect = 2e-05
Identities = 22/66 (33%), Positives = 35/66 (53%), Gaps = 4/66 (6%)
Frame = +2
Query: 290 LDKLKAERERGITIDIA----LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 457
LDKL+ ER RGIT+ ++ + Y + +ID PGH DF ++ + + +L+
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILL 154
Query: 458 VAAGTG 475
V A G
Sbjct: 155 VDASQG 160
Score = 25.0 bits (52), Expect = 7.9
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 5/47 (10%)
Frame = +3
Query: 138 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT-----IEKFEKEAQKWV 263
N VI H+D GKST + ++ G I++ ++K E E ++ +
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNFRNQFLDKLEVERRRGI 106
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 35.9 bits (79), Expect = 0.004
Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 2/79 (2%)
Frame = +2
Query: 245 GGPEMGKGSFKYAWVLDKLKAERERGITIDIALWK--FETSKYYVTIIDAPGHRDFIKNM 418
G + GK + A+ + + GIT I + F+ ++T +D PGH F
Sbjct: 178 GHVDHGKTTLLDAFRKSTIASTEHGGITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMR 237
Query: 419 ITGTSQADCAVLIVAAGTG 475
G + AD VL+VA G
Sbjct: 238 KRGANIADIVVLVVAGDDG 256
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.006
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 114 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 209
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 365 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 460
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.006
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 114 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 209
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 365 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 460
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 32.3 bits (70), Expect = 0.052
Identities = 28/81 (34%), Positives = 38/81 (46%), Gaps = 19/81 (23%)
Frame = +2
Query: 290 LDKLKAERERGITIDIA----LWK---------------FETSKYYVTIIDAPGHRDFIK 412
+D ++ ERE+GITI A W+ FE S Y + IID PGH DF
Sbjct: 102 MDFMELEREKGITIQSAATHCTWERTVDQIEANEKQKTDFEKS-YNINIIDTPGHIDFTI 160
Query: 413 NMITGTSQADCAVLIVAAGTG 475
+ D AVL++ A +G
Sbjct: 161 EVERALRVLDGAVLVLCAVSG 181
Score = 29.1 bits (62), Expect = 0.49
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 138 NIVVIGHVDSGKSTTTGHLIYKCGGI 215
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 31.1 bits (67), Expect = 0.12
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 368 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 463
+V+ +D PGH + M+ G + D A+L++A
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIA 140
Score = 25.0 bits (52), Expect = 7.9
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 108 PKMGKEKTHINIVVIGHVDSGKST 179
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.21
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 365 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 475
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 29.9 bits (64), Expect = 0.28
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
Frame = +2
Query: 293 DKLKAER-ERGITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 415
+K++A+ E+ + I+I + E +++ T+ID PG DFI N
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.37
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 377 IIDAPGHRDFIKNMITGTSQADCAVLIV 460
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.5 bits (58), Expect = 1.5
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +2
Query: 308 ERERGITID-----IALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 460
ERER ++I +A+ + + ID PGH DF+ + + +D VL+V
Sbjct: 185 ERERVMSIKSTPLTLAVSDMKGKTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVV 240
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,222,242
Number of Sequences: 5004
Number of extensions: 42399
Number of successful extensions: 166
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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