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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20222
         (581 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||...   130   1e-31
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb...    82   5e-17
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce...    78   8e-16
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s...    69   4e-13
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    52   5e-08
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe...    46   4e-06
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc...    41   2e-04
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa...    33   0.030
SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc...    31   0.16 
SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po...    29   0.65 
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos...    28   1.1  
SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomy...    27   1.5  
SPBC839.12 |rpc31||DNA-directed RNA polymerase III complex subun...    27   2.0  
SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S...    26   3.5  
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    26   4.6  
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S...    26   4.6  
SPBC530.09c |||cation dependent mannose-6-phosphate cargo recept...    25   6.1  
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch...    25   6.1  

>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 375

 Score =  130 bits (314), Expect = 1e-31
 Identities = 62/78 (79%), Positives = 62/78 (79%), Gaps = 1/78 (1%)
 Frame = +2

Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526
           PIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAP      RE      FETFN 
Sbjct: 70  PIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNA 129

Query: 527 PAMYVAIQAVLSLYASGR 580
           PA YVAIQAVLSLYASGR
Sbjct: 130 PAFYVAIQAVLSLYASGR 147



 Score = 77.8 bits (183), Expect = 1e-15
 Identities = 48/97 (49%), Positives = 56/97 (57%), Gaps = 3/97 (3%)
 Frame = +3

Query: 249 GKPRHQGVMVGMGQKDSYVGDEAQSTRGILTLKYPSNTESSLTGMTWRRFGIIPSTM--- 419
           G+PRH G+MVGMGQKDSYVGDEAQS RGILTLKYP   E  +         I   T    
Sbjct: 36  GRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYP--IEHGIVNNWDDMEKIWHHTFYNE 93

Query: 420 SCVSPPRNTQSCSLRLPQPKANREKMTQIISKHSTRP 530
             V+P  +    +     PK+NREKMTQII +    P
Sbjct: 94  LRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAP 130



 Score = 76.6 bits (180), Expect = 2e-15
 Identities = 33/37 (89%), Positives = 36/37 (97%)
 Frame = +1

Query: 148 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGSP 258
           +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVG P
Sbjct: 2   EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRP 38


>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 379

 Score = 82.2 bits (194), Expect = 5e-17
 Identities = 39/81 (48%), Positives = 54/81 (66%), Gaps = 2/81 (2%)
 Frame = +2

Query: 344 QIPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPSTQGQQREDDPDHF-ET 517
           Q PIE GI+ NW DME+IW + + + +L   PEEHP+LLTE P    + +E   ++F ET
Sbjct: 72  QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131

Query: 518 FNTPAMYVAIQAVLSLYASGR 580
            N PA+  ++Q VL+LYAS R
Sbjct: 132 LNVPALSFSLQPVLALYASAR 152



 Score = 46.8 bits (106), Expect = 2e-06
 Identities = 18/29 (62%), Positives = 23/29 (79%)
 Frame = +1

Query: 166 LVVDNGSGMCKAGFAGDDAPRAVFPSIVG 252
           + +DNGSG  KAGFAGDD P+ +FP+ VG
Sbjct: 12  ICIDNGSGFIKAGFAGDDIPKCLFPTCVG 40



 Score = 37.5 bits (83), Expect = 0.001
 Identities = 16/35 (45%), Positives = 24/35 (68%)
 Frame = +3

Query: 249 GKPRHQGVMVGMGQKDSYVGDEAQSTRGILTLKYP 353
           G+ +H+ VM    QKD +VG EAQ+ RG+L ++ P
Sbjct: 40  GRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRP 74


>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 427

 Score = 78.2 bits (184), Expect = 8e-16
 Identities = 35/76 (46%), Positives = 48/76 (63%), Gaps = 1/76 (1%)
 Frame = +2

Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526
           PI HG + NWD ME+ W  + +  LR  PE+H  LLTE P    + RE+  +  FE+FN 
Sbjct: 93  PIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNC 152

Query: 527 PAMYVAIQAVLSLYAS 574
             +Y+A+QAVL+L AS
Sbjct: 153 AGLYIAVQAVLALAAS 168



 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 14/30 (46%), Positives = 24/30 (80%)
 Frame = +1

Query: 166 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGS 255
           +++DNG+G  K G+AG+DAP  VFP+++ +
Sbjct: 8   IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT 37


>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
           subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score = 69.3 bits (162), Expect = 4e-13
 Identities = 32/77 (41%), Positives = 50/77 (64%), Gaps = 1/77 (1%)
 Frame = +2

Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526
           P+E+GI+ ++++M ++W +TF+ +L++ P    +LLTE P      RE   +  FE +  
Sbjct: 70  PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGF 129

Query: 527 PAMYVAIQAVLSLYASG 577
             +YVAIQAVLSLYA G
Sbjct: 130 GGVYVAIQAVLSLYAQG 146



 Score = 49.6 bits (113), Expect = 3e-07
 Identities = 22/39 (56%), Positives = 28/39 (71%)
 Frame = +1

Query: 154 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGSPAIRA 270
           E A +V+DNG+G  K G+A D+ PR  FPSIVG P +RA
Sbjct: 2   ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRA 40



 Score = 30.7 bits (66), Expect = 0.16
 Identities = 12/21 (57%), Positives = 16/21 (76%)
 Frame = +3

Query: 291 KDSYVGDEAQSTRGILTLKYP 353
           KD  VGDEA++ R +L +KYP
Sbjct: 50  KDVMVGDEAEAVRSLLQVKYP 70


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 52.4 bits (120), Expect = 5e-08
 Identities = 26/77 (33%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
 Frame = +2

Query: 353 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNTP 529
           I +G V NWD    +W +    +L+  P EHP+L+TE      + R    +  FE+   P
Sbjct: 69  IRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCP 128

Query: 530 AMYVAIQAVLSLYASGR 580
           A Y+A Q   + +ASG+
Sbjct: 129 ATYLAKQETCAAFASGK 145



 Score = 40.3 bits (90), Expect = 2e-04
 Identities = 16/34 (47%), Positives = 24/34 (70%)
 Frame = +1

Query: 151 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 252
           +EV+A+V+D GS   + GF+G+D P+ V PS  G
Sbjct: 9   DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42


>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 721

 Score = 46.0 bits (104), Expect = 4e-06
 Identities = 23/74 (31%), Positives = 39/74 (52%), Gaps = 1/74 (1%)
 Frame = +2

Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526
           P E  +++NWD ME++  +TF  +L +   EHP+ +TE  +     R    +  FE +N 
Sbjct: 86  PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144

Query: 527 PAMYVAIQAVLSLY 568
           P++   I  + S Y
Sbjct: 145 PSVAYGIDGLFSFY 158



 Score = 33.1 bits (72), Expect = 0.030
 Identities = 13/28 (46%), Positives = 20/28 (71%)
 Frame = +1

Query: 166 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 249
           LV+DNGS   +AG+ G+  P+ VF ++V
Sbjct: 27  LVIDNGSWQLRAGWGGEKDPKLVFDNLV 54


>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
           Arp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 430

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 20/75 (26%), Positives = 39/75 (52%), Gaps = 1/75 (1%)
 Frame = +2

Query: 359 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP-STQGQQREDDPDHFETFNTPAM 535
           +GI+ +W+     W      +L+V P E+ +++TE   + Q  +++     FE  + PA 
Sbjct: 74  NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133

Query: 536 YVAIQAVLSLYASGR 580
           Y+  QAV   +A+ +
Sbjct: 134 YLTKQAVCVAFANSK 148



 Score = 36.7 bits (81), Expect = 0.002
 Identities = 14/34 (41%), Positives = 23/34 (67%)
 Frame = +1

Query: 151 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 252
           EE+ +LV+D GS   + G+AG+++P  + PS  G
Sbjct: 8   EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41


>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
           Arp9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 523

 Score = 33.1 bits (72), Expect = 0.030
 Identities = 24/86 (27%), Positives = 37/86 (43%), Gaps = 5/86 (5%)
 Frame = +2

Query: 335 PDPQIPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPSTQGQQRE-DD 499
           P+   PI+ G V +W+ ++  W H  Y+ L   P +    +PV L          RE   
Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166

Query: 500 PDHFETFNTPAMYVAIQAVLSLYASG 577
              FE    P   +A + ++ LYA G
Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYAIG 192


>SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 457

 Score = 30.7 bits (66), Expect = 0.16
 Identities = 24/91 (26%), Positives = 43/91 (47%)
 Frame = -3

Query: 291 SVPYRPSRPDGGASHDRGEHGARSIISCETGLAHTGAIVYYQRGNFFVAHLELVKLVRCR 112
           SVPY PS+P+GGA    G H  R +++    L  T         N  V    + +L+  R
Sbjct: 87  SVPY-PSKPNGGAYQGIGFHYGRFVMNLRKALTST--------PNVTVTEATVNELL--R 135

Query: 111 CDKMKIKRPAVTANREKQIPIAGRCPCSTQC 19
            +  ++    VT++++ + P+  + P +  C
Sbjct: 136 DETGEVITGVVTSSKKSESPVEYKAPLTIVC 166


>SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1564

 Score = 28.7 bits (61), Expect = 0.65
 Identities = 17/62 (27%), Positives = 26/62 (41%)
 Frame = +3

Query: 327  RGILTLKYPSNTESSLTGMTWRRFGIIPSTMSCVSPPRNTQSCSLRLPQPKANREKMTQI 506
            RG L LK PS  E  L   +   F  +P  + C++P     S  +  P P      +  +
Sbjct: 863  RGFLNLK-PSELERELFSRSPDLFNCLPRLLCCIAPILQLLSALILAPWPSETPSLLAYM 921

Query: 507  IS 512
            I+
Sbjct: 922  IN 923


>SPAC20G8.05c |cdc15||cell division control protein
           Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 927

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 11/23 (47%), Positives = 14/23 (60%)
 Frame = -3

Query: 318 VPHLLHKSPSVPYRPSRPDGGAS 250
           +  L+ K+PS PY  SRP   AS
Sbjct: 306 IDQLISKAPSYPYSSSRPSASAS 328


>SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 348

 Score = 27.5 bits (58), Expect = 1.5
 Identities = 11/31 (35%), Positives = 19/31 (61%)
 Frame = +2

Query: 467 PSTQGQQREDDPDHFETFNTPAMYVAIQAVL 559
           P+++G      PD+ E F+ PA+YV ++  L
Sbjct: 32  PTSRGTVPHLTPDNVEEFDIPALYVGLEDCL 62


>SPBC839.12 |rpc31||DNA-directed RNA polymerase III complex subunit
           Rpc31|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 210

 Score = 27.1 bits (57), Expect = 2.0
 Identities = 20/72 (27%), Positives = 31/72 (43%), Gaps = 1/72 (1%)
 Frame = +2

Query: 317 TEHKRYPDPQIPIEHGIVTNWDDMEKIWHHTFYNELRVA-PEEHPVLLTEAPSTQGQQRE 493
           T  K YPD  IP++  I       E++    FY E+R +  +E    +   P +  Q+ E
Sbjct: 27  TPTKLYPDRDIPLQRSI-----RPEELLELRFYKEIRTSFLDESAFYIRRNPISVVQRNE 81

Query: 494 DDPDHFETFNTP 529
           D    +   N P
Sbjct: 82  DGLVRYSDRNKP 93


>SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase
           Mde10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 512

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 9/22 (40%), Positives = 15/22 (68%)
 Frame = -1

Query: 329 SCALCLISYIRVLLSHTDHHAL 264
           SC L + SY  ++L+H+D + L
Sbjct: 8   SCVLAVSSYAEIILAHSDENLL 29


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 12/23 (52%), Positives = 14/23 (60%)
 Frame = -2

Query: 193 TYRSHCLLPTRQLLRRTS*ISEI 125
           TYR H  +PT  LL  T  +SEI
Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190


>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
           Tor2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2337

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = -1

Query: 347 FEGQDTSCALCLISYIRVLLSHTDHHA 267
           +EG+DT+      +Y+R++L  TD  A
Sbjct: 74  YEGEDTTRITRFANYLRIILPGTDQKA 100


>SPBC530.09c |||cation dependent mannose-6-phosphate cargo receptor
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 296

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = +3

Query: 474 PKANREKMTQIISKHSTRPPCTSPS 548
           PK+N  +M+ +IS    R P T+PS
Sbjct: 148 PKSNHIRMSTLISFTCNRDPYTAPS 172


>SPCC417.08 |tef3||translation elongation factor
           eEF3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1047

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 11/29 (37%), Positives = 15/29 (51%)
 Frame = -3

Query: 261 GGASHDRGEHGARSIISCETGLAHTGAIV 175
           GGASH   E     + +CE  LA+   I+
Sbjct: 421 GGASHAEEEEEGEDLCNCEFSLAYGAKIL 449


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,699,078
Number of Sequences: 5004
Number of extensions: 61646
Number of successful extensions: 192
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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