BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20222 (581 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 130 1e-31 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 82 5e-17 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 78 8e-16 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 69 4e-13 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 52 5e-08 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 46 4e-06 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 41 2e-04 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 33 0.030 SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 31 0.16 SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po... 29 0.65 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 28 1.1 SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomy... 27 1.5 SPBC839.12 |rpc31||DNA-directed RNA polymerase III complex subun... 27 2.0 SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S... 26 3.5 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 4.6 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 4.6 SPBC530.09c |||cation dependent mannose-6-phosphate cargo recept... 25 6.1 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 25 6.1 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 130 bits (314), Expect = 1e-31 Identities = 62/78 (79%), Positives = 62/78 (79%), Gaps = 1/78 (1%) Frame = +2 Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526 PIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAP RE FETFN Sbjct: 70 PIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNA 129 Query: 527 PAMYVAIQAVLSLYASGR 580 PA YVAIQAVLSLYASGR Sbjct: 130 PAFYVAIQAVLSLYASGR 147 Score = 77.8 bits (183), Expect = 1e-15 Identities = 48/97 (49%), Positives = 56/97 (57%), Gaps = 3/97 (3%) Frame = +3 Query: 249 GKPRHQGVMVGMGQKDSYVGDEAQSTRGILTLKYPSNTESSLTGMTWRRFGIIPSTM--- 419 G+PRH G+MVGMGQKDSYVGDEAQS RGILTLKYP E + I T Sbjct: 36 GRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYP--IEHGIVNNWDDMEKIWHHTFYNE 93 Query: 420 SCVSPPRNTQSCSLRLPQPKANREKMTQIISKHSTRP 530 V+P + + PK+NREKMTQII + P Sbjct: 94 LRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAP 130 Score = 76.6 bits (180), Expect = 2e-15 Identities = 33/37 (89%), Positives = 36/37 (97%) Frame = +1 Query: 148 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGSP 258 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVG P Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRP 38 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 82.2 bits (194), Expect = 5e-17 Identities = 39/81 (48%), Positives = 54/81 (66%), Gaps = 2/81 (2%) Frame = +2 Query: 344 QIPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPSTQGQQREDDPDHF-ET 517 Q PIE GI+ NW DME+IW + + + +L PEEHP+LLTE P + +E ++F ET Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131 Query: 518 FNTPAMYVAIQAVLSLYASGR 580 N PA+ ++Q VL+LYAS R Sbjct: 132 LNVPALSFSLQPVLALYASAR 152 Score = 46.8 bits (106), Expect = 2e-06 Identities = 18/29 (62%), Positives = 23/29 (79%) Frame = +1 Query: 166 LVVDNGSGMCKAGFAGDDAPRAVFPSIVG 252 + +DNGSG KAGFAGDD P+ +FP+ VG Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVG 40 Score = 37.5 bits (83), Expect = 0.001 Identities = 16/35 (45%), Positives = 24/35 (68%) Frame = +3 Query: 249 GKPRHQGVMVGMGQKDSYVGDEAQSTRGILTLKYP 353 G+ +H+ VM QKD +VG EAQ+ RG+L ++ P Sbjct: 40 GRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRP 74 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 78.2 bits (184), Expect = 8e-16 Identities = 35/76 (46%), Positives = 48/76 (63%), Gaps = 1/76 (1%) Frame = +2 Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526 PI HG + NWD ME+ W + + LR PE+H LLTE P + RE+ + FE+FN Sbjct: 93 PIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNC 152 Query: 527 PAMYVAIQAVLSLYAS 574 +Y+A+QAVL+L AS Sbjct: 153 AGLYIAVQAVLALAAS 168 Score = 41.1 bits (92), Expect = 1e-04 Identities = 14/30 (46%), Positives = 24/30 (80%) Frame = +1 Query: 166 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGS 255 +++DNG+G K G+AG+DAP VFP+++ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT 37 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 69.3 bits (162), Expect = 4e-13 Identities = 32/77 (41%), Positives = 50/77 (64%), Gaps = 1/77 (1%) Frame = +2 Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526 P+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P RE + FE + Sbjct: 70 PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGF 129 Query: 527 PAMYVAIQAVLSLYASG 577 +YVAIQAVLSLYA G Sbjct: 130 GGVYVAIQAVLSLYAQG 146 Score = 49.6 bits (113), Expect = 3e-07 Identities = 22/39 (56%), Positives = 28/39 (71%) Frame = +1 Query: 154 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGSPAIRA 270 E A +V+DNG+G K G+A D+ PR FPSIVG P +RA Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRA 40 Score = 30.7 bits (66), Expect = 0.16 Identities = 12/21 (57%), Positives = 16/21 (76%) Frame = +3 Query: 291 KDSYVGDEAQSTRGILTLKYP 353 KD VGDEA++ R +L +KYP Sbjct: 50 KDVMVGDEAEAVRSLLQVKYP 70 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 52.4 bits (120), Expect = 5e-08 Identities = 26/77 (33%), Positives = 40/77 (51%), Gaps = 1/77 (1%) Frame = +2 Query: 353 IEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNTP 529 I +G V NWD +W + +L+ P EHP+L+TE + R + FE+ P Sbjct: 69 IRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMFESLRCP 128 Query: 530 AMYVAIQAVLSLYASGR 580 A Y+A Q + +ASG+ Sbjct: 129 ATYLAKQETCAAFASGK 145 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +1 Query: 151 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 252 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 46.0 bits (104), Expect = 4e-06 Identities = 23/74 (31%), Positives = 39/74 (52%), Gaps = 1/74 (1%) Frame = +2 Query: 350 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPSTQGQQREDDPD-HFETFNT 526 P E +++NWD ME++ +TF +L + EHP+ +TE + R + FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 527 PAMYVAIQAVLSLY 568 P++ I + S Y Sbjct: 145 PSVAYGIDGLFSFY 158 Score = 33.1 bits (72), Expect = 0.030 Identities = 13/28 (46%), Positives = 20/28 (71%) Frame = +1 Query: 166 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 249 LV+DNGS +AG+ G+ P+ VF ++V Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLV 54 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 40.7 bits (91), Expect = 2e-04 Identities = 20/75 (26%), Positives = 39/75 (52%), Gaps = 1/75 (1%) Frame = +2 Query: 359 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP-STQGQQREDDPDHFETFNTPAM 535 +GI+ +W+ W +L+V P E+ +++TE + Q +++ FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 536 YVAIQAVLSLYASGR 580 Y+ QAV +A+ + Sbjct: 134 YLTKQAVCVAFANSK 148 Score = 36.7 bits (81), Expect = 0.002 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +1 Query: 151 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 252 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 33.1 bits (72), Expect = 0.030 Identities = 24/86 (27%), Positives = 37/86 (43%), Gaps = 5/86 (5%) Frame = +2 Query: 335 PDPQIPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPSTQGQQRE-DD 499 P+ PI+ G V +W+ ++ W H Y+ L P + +PV L RE Sbjct: 108 PNEIQPIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELAT 166 Query: 500 PDHFETFNTPAMYVAIQAVLSLYASG 577 FE P +A + ++ LYA G Sbjct: 167 QFFFEECQVPGFTIAYEPLMGLYAIG 192 >SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 30.7 bits (66), Expect = 0.16 Identities = 24/91 (26%), Positives = 43/91 (47%) Frame = -3 Query: 291 SVPYRPSRPDGGASHDRGEHGARSIISCETGLAHTGAIVYYQRGNFFVAHLELVKLVRCR 112 SVPY PS+P+GGA G H R +++ L T N V + +L+ R Sbjct: 87 SVPY-PSKPNGGAYQGIGFHYGRFVMNLRKALTST--------PNVTVTEATVNELL--R 135 Query: 111 CDKMKIKRPAVTANREKQIPIAGRCPCSTQC 19 + ++ VT++++ + P+ + P + C Sbjct: 136 DETGEVITGVVTSSKKSESPVEYKAPLTIVC 166 >SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces pombe|chr 1|||Manual Length = 1564 Score = 28.7 bits (61), Expect = 0.65 Identities = 17/62 (27%), Positives = 26/62 (41%) Frame = +3 Query: 327 RGILTLKYPSNTESSLTGMTWRRFGIIPSTMSCVSPPRNTQSCSLRLPQPKANREKMTQI 506 RG L LK PS E L + F +P + C++P S + P P + + Sbjct: 863 RGFLNLK-PSELERELFSRSPDLFNCLPRLLCCIAPILQLLSALILAPWPSETPSLLAYM 921 Query: 507 IS 512 I+ Sbjct: 922 IN 923 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 27.9 bits (59), Expect = 1.1 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = -3 Query: 318 VPHLLHKSPSVPYRPSRPDGGAS 250 + L+ K+PS PY SRP AS Sbjct: 306 IDQLISKAPSYPYSSSRPSASAS 328 >SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 27.5 bits (58), Expect = 1.5 Identities = 11/31 (35%), Positives = 19/31 (61%) Frame = +2 Query: 467 PSTQGQQREDDPDHFETFNTPAMYVAIQAVL 559 P+++G PD+ E F+ PA+YV ++ L Sbjct: 32 PTSRGTVPHLTPDNVEEFDIPALYVGLEDCL 62 >SPBC839.12 |rpc31||DNA-directed RNA polymerase III complex subunit Rpc31|Schizosaccharomyces pombe|chr 2|||Manual Length = 210 Score = 27.1 bits (57), Expect = 2.0 Identities = 20/72 (27%), Positives = 31/72 (43%), Gaps = 1/72 (1%) Frame = +2 Query: 317 TEHKRYPDPQIPIEHGIVTNWDDMEKIWHHTFYNELRVA-PEEHPVLLTEAPSTQGQQRE 493 T K YPD IP++ I E++ FY E+R + +E + P + Q+ E Sbjct: 27 TPTKLYPDRDIPLQRSI-----RPEELLELRFYKEIRTSFLDESAFYIRRNPISVVQRNE 81 Query: 494 DDPDHFETFNTP 529 D + N P Sbjct: 82 DGLVRYSDRNKP 93 >SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|Schizosaccharomyces pombe|chr 1|||Manual Length = 512 Score = 26.2 bits (55), Expect = 3.5 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = -1 Query: 329 SCALCLISYIRVLLSHTDHHAL 264 SC L + SY ++L+H+D + L Sbjct: 8 SCVLAVSSYAEIILAHSDENLL 29 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 4.6 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -2 Query: 193 TYRSHCLLPTRQLLRRTS*ISEI 125 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 25.8 bits (54), Expect = 4.6 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = -1 Query: 347 FEGQDTSCALCLISYIRVLLSHTDHHA 267 +EG+DT+ +Y+R++L TD A Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100 >SPBC530.09c |||cation dependent mannose-6-phosphate cargo receptor |Schizosaccharomyces pombe|chr 2|||Manual Length = 296 Score = 25.4 bits (53), Expect = 6.1 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +3 Query: 474 PKANREKMTQIISKHSTRPPCTSPS 548 PK+N +M+ +IS R P T+PS Sbjct: 148 PKSNHIRMSTLISFTCNRDPYTAPS 172 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 25.4 bits (53), Expect = 6.1 Identities = 11/29 (37%), Positives = 15/29 (51%) Frame = -3 Query: 261 GGASHDRGEHGARSIISCETGLAHTGAIV 175 GGASH E + +CE LA+ I+ Sbjct: 421 GGASHAEEEEEGEDLCNCEFSLAYGAKIL 449 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,699,078 Number of Sequences: 5004 Number of extensions: 61646 Number of successful extensions: 192 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 250133048 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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