Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20221
(696 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 153 8e-38
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 153 8e-38
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 153 8e-38
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 153 8e-38
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 97 7e-21
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 94 9e-20
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 71 6e-13
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 68 5e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 38 0.005
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 38 0.005
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 36 0.034
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 36 0.034
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 35 0.045
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 33 0.14
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 33 0.14
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 33 0.14
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.18
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 33 0.18
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.24
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 33 0.24
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.24
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.7
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.7
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.7
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.2
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.2
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.1
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 28 5.1
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.8
At5g13650.2 68418.m01585 elongation factor family protein contai... 28 6.8
At5g13650.1 68418.m01584 elongation factor family protein contai... 28 6.8
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 6.8
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 27 9.0
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 9.0
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 9.0
At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-pa... 27 9.0
At3g05470.1 68416.m00599 formin homology 2 domain-containing pro... 27 9.0
At1g02010.1 68414.m00119 cytokinesis-related Sec1 protein, putat... 27 9.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 153 bits (372), Expect = 8e-38
Identities = 71/81 (87%), Positives = 77/81 (95%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
RGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGIS
Sbjct: 69 RGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGIS 128
Query: 435 KNGQTREHALLAFTLGVKQLI 497
K+GQTREHALLAFTLGVKQ+I
Sbjct: 129 KDGQTREHALLAFTLGVKQMI 149
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = +2
Query: 509 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMFGAFNQMPW 679
KMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM + W
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDW 210
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 153 bits (372), Expect = 8e-38
Identities = 71/81 (87%), Positives = 77/81 (95%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
RGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGIS
Sbjct: 69 RGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGIS 128
Query: 435 KNGQTREHALLAFTLGVKQLI 497
K+GQTREHALLAFTLGVKQ+I
Sbjct: 129 KDGQTREHALLAFTLGVKQMI 149
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = +2
Query: 509 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMFGAFNQMPW 679
KMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM + W
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDW 210
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 153 bits (372), Expect = 8e-38
Identities = 71/81 (87%), Positives = 77/81 (95%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
RGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGIS
Sbjct: 69 RGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGIS 128
Query: 435 KNGQTREHALLAFTLGVKQLI 497
K+GQTREHALLAFTLGVKQ+I
Sbjct: 129 KDGQTREHALLAFTLGVKQMI 149
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = +2
Query: 509 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMFGAFNQMPW 679
KMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM + W
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDW 210
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 153 bits (372), Expect = 8e-38
Identities = 71/81 (87%), Positives = 77/81 (95%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
RGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGIS
Sbjct: 69 RGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGIS 128
Query: 435 KNGQTREHALLAFTLGVKQLI 497
K+GQTREHALLAFTLGVKQ+I
Sbjct: 129 KDGQTREHALLAFTLGVKQMI 149
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 78.6 bits (185), Expect = 4e-15
Identities = 33/57 (57%), Positives = 42/57 (73%)
Frame = +2
Query: 509 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMFGAFNQMPW 679
KMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM + W
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDW 210
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 97.5 bits (232), Expect = 7e-21
Identities = 45/86 (52%), Positives = 59/86 (68%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
+G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G
Sbjct: 163 KGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYE 222
Query: 435 KNGQTREHALLAFTLGVKQLIVGVKK 512
+ GQTREH LA TLGV +LIV V K
Sbjct: 223 RGGQTREHVQLAKTLGVSKLIVVVNK 248
Score = 68.1 bits (159), Expect = 5e-12
Identities = 28/64 (43%), Positives = 46/64 (71%)
Frame = +1
Query: 61 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 240
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 241 KAER 252
+ ER
Sbjct: 158 EEER 161
Score = 42.3 bits (95), Expect = 3e-04
Identities = 23/60 (38%), Positives = 34/60 (56%), Gaps = 3/60 (5%)
Frame = +2
Query: 509 KMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMFGAFNQ--MPW 679
KMD +S+ R++EI++++ ++K GYN V F+PISG G NM Q PW
Sbjct: 248 KMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEICPW 307
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 93.9 bits (223), Expect = 9e-20
Identities = 41/87 (47%), Positives = 61/87 (70%), Gaps = 1/87 (1%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
RGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG
Sbjct: 301 RGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFD 360
Query: 435 K-NGQTREHALLAFTLGVKQLIVGVKK 512
GQTREHA + GV+Q+IV + K
Sbjct: 361 NLKGQTREHARVLRGFGVEQVIVAINK 387
Score = 76.2 bits (179), Expect = 2e-14
Identities = 31/63 (49%), Positives = 46/63 (73%)
Frame = +1
Query: 67 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 246
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 247 ERD 255
ER+
Sbjct: 298 ERE 300
Score = 33.9 bits (74), Expect = 0.10
Identities = 12/43 (27%), Positives = 26/43 (60%)
Frame = +2
Query: 533 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMFGA 661
YS+ RF+ IK+ V S+++ + +++ ++P+S N+ A
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAA 435
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 71.3 bits (167), Expect = 6e-13
Identities = 36/86 (41%), Positives = 52/86 (60%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G
Sbjct: 126 RGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP---- 181
Query: 435 KNGQTREHALLAFTLGVKQLIVGVKK 512
QT+EH LLA +GV ++V + K
Sbjct: 182 ---QTKEHILLAKQVGVPDMVVFLNK 204
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 49 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 183
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 68.1 bits (159), Expect = 5e-12
Identities = 36/86 (41%), Positives = 51/86 (59%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 114 RGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP---- 169
Query: 435 KNGQTREHALLAFTLGVKQLIVGVKK 512
QT+EH LLA +GV L+ + K
Sbjct: 170 ---QTKEHILLARQVGVPSLVCFLNK 192
Score = 32.7 bits (71), Expect = 0.24
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 58 KEKTHINIVVIGHVDSGKSTTT 123
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 38.3 bits (85), Expect = 0.005
Identities = 27/91 (29%), Positives = 38/91 (41%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 435 KNGQTREHALLAFTLGVKQLIVGVKKWIPLN 527
+ Q R + + K +G W LN
Sbjct: 177 VDRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 38.3 bits (85), Expect = 0.005
Identities = 27/91 (29%), Positives = 38/91 (41%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 434
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 435 KNGQTREHALLAFTLGVKQLIVGVKKWIPLN 527
+ Q R + + K +G W LN
Sbjct: 177 VDRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 35.5 bits (78), Expect = 0.034
Identities = 18/53 (33%), Positives = 25/53 (47%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 193 KAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 35.5 bits (78), Expect = 0.034
Identities = 18/53 (33%), Positives = 25/53 (47%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 193 KAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 35.1 bits (77), Expect = 0.045
Identities = 17/53 (32%), Positives = 28/53 (52%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
Score = 32.7 bits (71), Expect = 0.24
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGG 150
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 33.5 bits (73), Expect = 0.14
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +3
Query: 294 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 473
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 474 TLGVKQLIV 500
+ +K +I+
Sbjct: 178 MMRLKHIII 186
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 33.5 bits (73), Expect = 0.14
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +3
Query: 294 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 473
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 474 TLGVKQLIV 500
+ +K +I+
Sbjct: 178 MMRLKHIII 186
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 33.5 bits (73), Expect = 0.14
Identities = 26/99 (26%), Positives = 47/99 (47%), Gaps = 4/99 (4%)
Frame = +3
Query: 288 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 455
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 456 HALLAFTLGVKQLIVGVKKWIPLNHHTVSPDLRKSRRKY 572
H + +K +I+ ++ I L V+ + ++ +K+
Sbjct: 166 HLAAVEIMQLKHIII-LQNKIDLIQENVAINQHEAIQKF 203
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +3
Query: 291 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
E S Y + +ID PGH DF + S A+L+V A G
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG 171
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +1
Query: 40 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 159
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 33.1 bits (72), Expect = 0.18
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 4/57 (7%)
Frame = +3
Query: 255 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
RGITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 28.3 bits (60), Expect = 5.1
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 174
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.24
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 309 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 32.7 bits (71), Expect = 0.24
Identities = 19/65 (29%), Positives = 31/65 (47%)
Frame = +3
Query: 306 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 485
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 486 KQLIV 500
K +I+
Sbjct: 174 KDIII 178
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.24
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCG 147
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
Score = 31.1 bits (67), Expect = 0.73
Identities = 18/53 (33%), Positives = 24/53 (45%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
RGITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 145 RGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 315 IIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 315 IIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 315 IIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 297 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 398
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 2.9
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +3
Query: 309 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.1
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +2
Query: 536 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 625
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 28.3 bits (60), Expect = 5.1
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = -2
Query: 401 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCDTTSRSAFSLSN 228
++D+HS +L+ D + D + ++ N+VL F P+ D T +R A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 542 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 652
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 27.9 bits (59), Expect = 6.8
Identities = 18/53 (33%), Positives = 23/53 (43%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 182
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 27.9 bits (59), Expect = 6.8
Identities = 18/53 (33%), Positives = 23/53 (43%)
Frame = +3
Query: 255 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 181
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 318 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 27.5 bits (58), Expect = 9.0
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = +2
Query: 479 RCQTAHRRSKKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 643
R T+H + K ++STE E KEV ++K +GY+ + V ++ +G
Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYG 366
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 518 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 631
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2
Query: 518 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 631
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-patch
domain-containing protein / RNA recognition motif
(RRM)-containing protein KIAA0122 gene , Homo sapiens,
EMBL:HSDKG02; contains Pfam profiles PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF01585: G-patch domain, weak hit to PF00641: Zn-finger
in Ran binding protein and others
Length = 1105
Score = 27.5 bits (58), Expect = 9.0
Identities = 18/51 (35%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = +3
Query: 531 HTVSPDLRKSRRKYPHTSRRLATTQLLSL--SCPFLDGTETTCLEPSTKCL 677
H DLR R K+ H SR A S+ + L+ T T LE + K L
Sbjct: 481 HAPIKDLRLVRDKFTHVSRGFAFVHFYSVEDATKALEATNRTALERNGKIL 531
>At3g05470.1 68416.m00599 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 884
Score = 27.5 bits (58), Expect = 9.0
Identities = 24/85 (28%), Positives = 35/85 (41%)
Frame = -2
Query: 401 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCDTTSRSAFSLSNTQ 222
S D+ S S+ G + +S + S + V +S E +D SRS F +S +
Sbjct: 308 SDDDESFHSVGGGSQYSNPRLSNASSASGSVNVGSSQRFSEHKLDIPECSRSDFGISVSA 367
Query: 221 AYLKDPLPISWASFSNFSMVRLSIP 147
P P FSN + LS P
Sbjct: 368 PPPPPPPPPPLPQFSNKRIHTLSSP 392
>At1g02010.1 68414.m00119 cytokinesis-related Sec1 protein, putative
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family; non-consensus GC
donor splice site at exon boundary 46833
Length = 676
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -3
Query: 667 VEGSKHVVSVPSRNGHESDSSWVV 596
+EG+KHV+ VPS+ G + +V
Sbjct: 283 MEGNKHVIEVPSKTGGPPEKKEIV 306
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,901,870
Number of Sequences: 28952
Number of extensions: 339752
Number of successful extensions: 1161
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 1093
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1157
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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