Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20219
(742 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces ... 89 8e-19
SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 84 2e-17
SPAC2F3.08 |sut1||alpha-glucoside transporter |Schizosaccharomyc... 28 1.6
SPBC18E5.12c |mas2|SPBC23G7.02c|mitochondrial processing peptida... 27 2.1
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 27 2.8
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 25 8.6
>SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 409
Score = 88.6 bits (210), Expect = 8e-19
Identities = 41/92 (44%), Positives = 63/92 (68%), Gaps = 1/92 (1%)
Frame = +2
Query: 254 EDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSR-GLNHEYAPISGEATYTDAVAK 430
+D PKKVN+ VGAYRDD GKP++LP+V+KA +I+ + NHEY PI+G +T A A+
Sbjct: 26 QDEDPKKVNMSVGAYRDDTGKPWILPAVKKASKIVEEQASFNHEYLPIAGLPRFTKAAAE 85
Query: 431 LAFGEDSPVIKNKSNCTVQTLSGTGASALDSS 526
+ F + ++ ++Q++SGTGA+ L +S
Sbjct: 86 VLFRPNPHLLSEDRVASMQSVSGTGANFLAAS 117
Score = 43.2 bits (97), Expect = 4e-05
Identities = 24/79 (30%), Positives = 34/79 (43%), Gaps = 3/79 (3%)
Frame = +1
Query: 514 LGLEFITKHYAK--AKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALED 687
L FI Y K +++ PTW H + L + Y Y+D K FD +G L
Sbjct: 114 LAASFIETFYVKHTGAHVYISNPTWPVHRTLWEKLGVTVDTYPYWDAKNRSFDYEGMLST 173
Query: 688 ISKFPKVP-YSVARVAHNP 741
I P+ + + AHNP
Sbjct: 174 IKSAPEGSIFLLHACAHNP 192
>SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 437
Score = 84.2 bits (199), Expect = 2e-17
Identities = 43/86 (50%), Positives = 60/86 (69%)
Frame = +2
Query: 251 QEDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLNHEYAPISGEATYTDAVAK 430
++D KK+NLG G YRDD GKP+VLPSVR+AE L S+ L+ EYAPI+G ++ K
Sbjct: 59 KKDGDVKKMNLGAGTYRDDAGKPYVLPSVRQAETELLSQKLDKEYAPITGIPSFRVQATK 118
Query: 431 LAFGEDSPVIKNKSNCTVQTLSGTGA 508
LA+G+ IK++ + Q++SGTGA
Sbjct: 119 LAYGDVYESIKDRL-VSAQSISGTGA 143
Score = 60.5 bits (140), Expect = 2e-10
Identities = 27/77 (35%), Positives = 41/77 (53%), Gaps = 1/77 (1%)
Frame = +1
Query: 514 LGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDIS 693
+ F+ Y +K I++ PTWGNH + + L K Y+Y+DP T G D++G L D++
Sbjct: 146 IAANFLASFYP-SKTIYVSDPTWGNHKNVFSRAGLTVKSYKYYDPATRGLDIKGMLSDLT 204
Query: 694 KFPKVPYSVARV-AHNP 741
P + AHNP
Sbjct: 205 SAPDGSIILLHACAHNP 221
Score = 39.9 bits (89), Expect = 4e-04
Identities = 17/27 (62%), Positives = 20/27 (74%)
Frame = +3
Query: 195 WNNVQMGPPDVILGITEAYKRTHILKK 275
W +V MGPPD I GITEAYK+ +KK
Sbjct: 40 WADVPMGPPDPIFGITEAYKKDGDVKK 66
>SPAC2F3.08 |sut1||alpha-glucoside transporter |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 553
Score = 27.9 bits (59), Expect = 1.6
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = -2
Query: 639 VTVFLVRQVEGVADLRVVSPSRRREPDLLCLSVVFRYELESKAEAP 502
+T L++ + G+ RV S RR P +LC S++ + L AP
Sbjct: 80 LTGILIQPIAGILSDRVNSRIGRRRPFMLCASLLGTFSLFLMGWAP 125
>SPBC18E5.12c |mas2|SPBC23G7.02c|mitochondrial processing peptidase
complex alpha subunit Mas2|Schizosaccharomyces pombe|chr
2|||Manual
Length = 494
Score = 27.5 bits (58), Expect = 2.1
Identities = 24/75 (32%), Positives = 31/75 (41%), Gaps = 1/75 (1%)
Frame = +3
Query: 294 HIEMMRENHLF-CLLSERQKKFSIVEDSIMSMLPSVVRPHILML*LNWLLEKTALSSKIR 470
H+ + N L CLL K I SI L RP L L + ++ A K
Sbjct: 186 HVTAFQNNTLGNCLLCTPDKVNGITATSIREYLKYFYRPEHLTLAYAGIPQEIA---KEI 242
Query: 471 ATVLYRHCPALALPP 515
LY H P+ +LPP
Sbjct: 243 TKELYGHLPSSSLPP 257
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 27.1 bits (57), Expect = 2.8
Identities = 17/63 (26%), Positives = 27/63 (42%)
Frame = +2
Query: 458 IKNKSNCTVQTLSGTGASALDSSS*RNTTXXXXXXXXXXXXXETTRKSATPSTCRTRNTV 637
+ + S+ T S +G SAL SSS TT TT + T + R T+
Sbjct: 528 VSSFSSSPSPTSSFSGTSALSSSSNEETTTTTQVTYTTSPEETTTTMTTTTCSSRPEETI 587
Query: 638 TSI 646
+++
Sbjct: 588 STV 590
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 25.4 bits (53), Expect = 8.6
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 455 VIKNKSNCTVQTLSGTGASALDSSS*RNT 541
VI N + T ++ GAS L+SSS NT
Sbjct: 217 VITNVDSTTTSVINYIGASTLESSSLTNT 245
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,392,634
Number of Sequences: 5004
Number of extensions: 76240
Number of successful extensions: 230
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 229
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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