Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20219
(742 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast... 91 9e-19
At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast... 91 9e-19
At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondri... 89 2e-18
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast... 82 3e-16
At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic... 81 8e-16
At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic... 77 9e-15
At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic... 75 7e-14
At4g37235.1 68417.m05271 expressed protein 30 1.4
At3g54000.3 68416.m05969 expressed protein 29 4.3
At3g54000.2 68416.m05968 expressed protein 29 4.3
At3g54000.1 68416.m05970 expressed protein 29 4.3
At1g70810.1 68414.m08168 C2 domain-containing protein similar to... 28 5.7
At3g08500.1 68416.m00985 myb family transcription factor (MYB83)... 28 7.5
At1g75530.1 68414.m08778 forkhead-associated domain-containing p... 28 7.5
At3g26140.1 68416.m03261 glycosyl hydrolase family 5 protein / c... 27 9.9
>At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 90.6 bits (215), Expect = 9e-19
Identities = 45/91 (49%), Positives = 60/91 (65%)
Frame = +2
Query: 245 SIQEDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLNHEYAPISGEATYTDAV 424
+ + DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG N EY PI G A + A
Sbjct: 70 AFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGDNKEYLPIEGLAAFNKAT 129
Query: 425 AKLAFGEDSPVIKNKSNCTVQTLSGTGASAL 517
A+L FG PVIK + T+Q LSGTG+ L
Sbjct: 130 AELLFGAGHPVIKEQRVATIQGLSGTGSLRL 160
Score = 68.1 bits (159), Expect = 6e-12
Identities = 31/78 (39%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = +1
Query: 511 RLGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDI 690
RL I +++ AK + + +PTWGNH I N +P +YRY+DPKT G D +G + DI
Sbjct: 159 RLAAALIERYFPGAKVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEGMIADI 217
Query: 691 SKFPKVPYSVAR-VAHNP 741
+ P+ + + AHNP
Sbjct: 218 KEAPEGSFILLHGCAHNP 235
Score = 28.7 bits (61), Expect = 4.3
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +3
Query: 195 WNNVQMGPPDVILGITEAYK 254
+ + M PPD ILG++EA+K
Sbjct: 53 FEGITMAPPDPILGVSEAFK 72
>At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 90.6 bits (215), Expect = 9e-19
Identities = 45/91 (49%), Positives = 60/91 (65%)
Frame = +2
Query: 245 SIQEDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLNHEYAPISGEATYTDAV 424
+ + DT+ K+NLGVGAYR +E +P+VL V+KAE ++ RG N EY PI G A + A
Sbjct: 70 AFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGDNKEYLPIEGLAAFNKAT 129
Query: 425 AKLAFGEDSPVIKNKSNCTVQTLSGTGASAL 517
A+L FG PVIK + T+Q LSGTG+ L
Sbjct: 130 AELLFGAGHPVIKEQRVATIQGLSGTGSLRL 160
Score = 68.1 bits (159), Expect = 6e-12
Identities = 31/78 (39%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = +1
Query: 511 RLGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDI 690
RL I +++ AK + + +PTWGNH I N +P +YRY+DPKT G D +G + DI
Sbjct: 159 RLAAALIERYFPGAKVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEGMIADI 217
Query: 691 SKFPKVPYSVAR-VAHNP 741
+ P+ + + AHNP
Sbjct: 218 KEAPEGSFILLHGCAHNP 235
Score = 28.7 bits (61), Expect = 4.3
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +3
Query: 195 WNNVQMGPPDVILGITEAYK 254
+ + M PPD ILG++EA+K
Sbjct: 53 FEGITMAPPDPILGVSEAFK 72
>At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondrial
/ transaminase A (ASP1) identical to SP|P46643 Aspartate
aminotransferase, mitochondrial precursor (EC 2.6.1.1)
(Transaminase A) {Arabidopsis thaliana}
Length = 430
Score = 89.4 bits (212), Expect = 2e-18
Identities = 47/87 (54%), Positives = 57/87 (65%)
Frame = +2
Query: 257 DTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLNHEYAPISGEATYTDAVAKLA 436
D P+KVN+GVGAYRDD GKP VL VR+AE+ L + EY P+ G A D KLA
Sbjct: 54 DPSPEKVNVGVGAYRDDNGKPVVLECVREAEKRL-AGSTFMEYLPMGGSAKMVDLTLKLA 112
Query: 437 FGEDSPVIKNKSNCTVQTLSGTGASAL 517
+G++S IK+K VQTLSGTGA L
Sbjct: 113 YGDNSEFIKDKRIAAVQTLSGTGACRL 139
Score = 61.7 bits (143), Expect = 5e-10
Identities = 26/78 (33%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
Frame = +1
Query: 511 RLGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDI 690
RL +F K ++ +I++P PTW NH I +P K Y Y+ P+T G D ++D+
Sbjct: 138 RLFADF-QKRFSPGSQIYIPVPTWSNHHNIWKDAQVPQKTYHYYHPETKGLDFSALMDDV 196
Query: 691 SKFPKVPYSVARV-AHNP 741
P+ + + AHNP
Sbjct: 197 KNAPEGSFFLLHACAHNP 214
Score = 40.7 bits (91), Expect = 0.001
Identities = 15/27 (55%), Positives = 21/27 (77%)
Frame = +3
Query: 171 GLRASSTWWNNVQMGPPDVILGITEAY 251
GLR+ S+WW +V+ P D ILG+TEA+
Sbjct: 25 GLRSMSSWWKSVEPAPKDPILGVTEAF 51
>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
transaminase A (ASP3) (YLS4) identical to SP|P46644
Aspartate aminotransferase, chloroplast precursor (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
identical to cDNA YLS4 mRNA for aspartate
aminotransferase (ASP3), partial cds GI:13122285
Length = 449
Score = 82.2 bits (194), Expect = 3e-16
Identities = 43/86 (50%), Positives = 54/86 (62%), Gaps = 1/86 (1%)
Frame = +2
Query: 254 EDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRGLNHEYAPISGEATYTDAVAK 430
+D P K+NLGVGAYR +EGKP VL VRKAE ++++ R EY PI G + AK
Sbjct: 69 KDPSPVKLNLGVGAYRTEEGKPLVLNVVRKAEQQLINDRTRIKEYLPIVGLVEFNKLSAK 128
Query: 431 LAFGEDSPVIKNKSNCTVQTLSGTGA 508
L G DSP I+ TV+ LSGTG+
Sbjct: 129 LILGADSPAIRENRITTVECLSGTGS 154
Score = 73.7 bits (173), Expect = 1e-13
Identities = 36/78 (46%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
Frame = +1
Query: 511 RLGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDI 690
R+G EF+ KHY + K I++ PTWGNHP+I L K YRY+DP T G + QG LED+
Sbjct: 156 RVGGEFLAKHYHQ-KTIYITQPTWGNHPKIFTLAGLTVKTYRYYDPATRGLNFQGLLEDL 214
Query: 691 -SKFPKVPYSVARVAHNP 741
+ P + AHNP
Sbjct: 215 GAAAPGSIVLLHACAHNP 232
>At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic
isozyme 1 / transaminase A (ASP2) identical to SP|P46645
Aspartate aminotransferase, cytoplasmic isozyme 1 (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana}
Length = 405
Score = 81.0 bits (191), Expect = 8e-16
Identities = 43/85 (50%), Positives = 52/85 (61%), Gaps = 1/85 (1%)
Frame = +2
Query: 257 DTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRGLNHEYAPISGEATYTDAVAKL 433
D P K+NLGVGAYR +EGKP VL VRKAE ++++ EY PI G + + AKL
Sbjct: 26 DPSPVKINLGVGAYRTEEGKPLVLDVVRKAEQQLVNDPSRVKEYIPIVGISDFNKLSAKL 85
Query: 434 AFGEDSPVIKNKSNCTVQTLSGTGA 508
G DSP I TVQ LSGTG+
Sbjct: 86 ILGADSPAITESRVTTVQCLSGTGS 110
Score = 74.1 bits (174), Expect = 9e-14
Identities = 33/78 (42%), Positives = 47/78 (60%), Gaps = 1/78 (1%)
Frame = +1
Query: 511 RLGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDI 690
R+G EF+ HY ++ I++P PTWGNHP++ N L + +RY+DP T G D +G LED+
Sbjct: 112 RVGAEFLKTHYHQSV-IYIPKPTWGNHPKVFNLAGLSVEYFRYYDPATRGLDFKGLLEDL 170
Query: 691 SKFPKVPYSVARV-AHNP 741
P + AHNP
Sbjct: 171 GAAPSGAIVLLHACAHNP 188
>At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 403
Score = 77.4 bits (182), Expect = 9e-15
Identities = 38/86 (44%), Positives = 52/86 (60%)
Frame = +2
Query: 251 QEDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAEEILHSRGLNHEYAPISGEATYTDAVAK 430
++D P K+NL G YR +EGKP VL VR+AE+ L + L+ EY P++G + K
Sbjct: 24 RDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQL-ANDLDKEYLPLNGLPEFNKLSTK 82
Query: 431 LAFGEDSPVIKNKSNCTVQTLSGTGA 508
L G+DSP +K T Q LSGTG+
Sbjct: 83 LILGDDSPALKENRVVTTQCLSGTGS 108
Score = 72.1 bits (169), Expect = 4e-13
Identities = 34/78 (43%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = +1
Query: 511 RLGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDI 690
R+G EF+ H K I++P PTWGNHP+I L + +RY+DPK+ G D +G LED+
Sbjct: 110 RVGAEFLATHN-KESVIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLDFKGMLEDL 168
Query: 691 SKFPKVPYSVARV-AHNP 741
P V + AHNP
Sbjct: 169 GAAPPGAIVVLQACAHNP 186
>At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 405
Score = 74.5 bits (175), Expect = 7e-14
Identities = 36/87 (41%), Positives = 51/87 (58%), Gaps = 1/87 (1%)
Frame = +2
Query: 251 QEDTHPKKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHSRGLNHEYAPISGEATYTDAVA 427
++D P K+NL G YR +EGKP VL VR+AE ++ + + EY P++G +
Sbjct: 24 RDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQLANDLSRDKEYLPLNGLPEFNKLST 83
Query: 428 KLAFGEDSPVIKNKSNCTVQTLSGTGA 508
KL G+DSP +K T Q LSGTG+
Sbjct: 84 KLILGDDSPALKENRVVTTQCLSGTGS 110
Score = 72.1 bits (169), Expect = 4e-13
Identities = 34/78 (43%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = +1
Query: 511 RLGLEFITKHYAKAKEIWLPTPTWGNHPQICNTLNLPHKKYRYFDPKTNGFDLQGALEDI 690
R+G EF+ H K I++P PTWGNHP+I L + +RY+DPK+ G D +G LED+
Sbjct: 112 RVGAEFLATHN-KESVIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLDFKGMLEDL 170
Query: 691 SKFPKVPYSVARV-AHNP 741
P V + AHNP
Sbjct: 171 GAAPPGAIVVLQACAHNP 188
>At4g37235.1 68417.m05271 expressed protein
Length = 152
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 4/44 (9%)
Frame = -1
Query: 496 GQCLYSTVALIF----DDRAVFSKSQFSYSISICGLTTDGSILM 377
GQ L+S+ +L+F DD ++ + F Y +++ GL T S+ +
Sbjct: 19 GQTLFSSASLLFMCFNDDEDFYAYTTFCYLVTVMGLVTPWSVTL 62
>At3g54000.3 68416.m05969 expressed protein
Length = 301
Score = 28.7 bits (61), Expect = 4.3
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = -1
Query: 703 SGILIYPRVLPANRNHWSWDRSNGISCAAS*GCC 602
SG + PA +H +W+ + C A GCC
Sbjct: 87 SGGVCGNHAFPAGNDHKAWEMNRSPPCVAGTGCC 120
>At3g54000.2 68416.m05968 expressed protein
Length = 301
Score = 28.7 bits (61), Expect = 4.3
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = -1
Query: 703 SGILIYPRVLPANRNHWSWDRSNGISCAAS*GCC 602
SG + PA +H +W+ + C A GCC
Sbjct: 87 SGGVCGNHAFPAGNDHKAWEMNRSPPCVAGTGCC 120
>At3g54000.1 68416.m05970 expressed protein
Length = 352
Score = 28.7 bits (61), Expect = 4.3
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = -1
Query: 703 SGILIYPRVLPANRNHWSWDRSNGISCAAS*GCC 602
SG + PA +H +W+ + C A GCC
Sbjct: 87 SGGVCGNHAFPAGNDHKAWEMNRSPPCVAGTGCC 120
>At1g70810.1 68414.m08168 C2 domain-containing protein similar to
zinc finger and C2 domain protein GI:9957238 from
[Arabidopsis thaliana]
Length = 165
Score = 28.3 bits (60), Expect = 5.7
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -1
Query: 85 LSNTGKIITNYVVLLKDEEC 26
+SN GKI+ N ++LL++ EC
Sbjct: 128 VSNNGKIVQNMILLLRNVEC 147
>At3g08500.1 68416.m00985 myb family transcription factor (MYB83)
contains Pfam profile: PF00249: Myb-like DNA-binding
domain
Length = 343
Score = 27.9 bits (59), Expect = 7.5
Identities = 11/42 (26%), Positives = 23/42 (54%)
Frame = +1
Query: 595 QICNTLNLPHKKYRYFDPKTNGFDLQGALEDISKFPKVPYSV 720
++ N L + + + +PK +DL G +++ S FP + + V
Sbjct: 301 KVGNVLGIENGSWEIENPKIGDWDLDGLIDNNSSFPFLDFQV 342
>At1g75530.1 68414.m08778 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 555
Score = 27.9 bits (59), Expect = 7.5
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +1
Query: 556 EIWLPTPTWGNHPQICN 606
EIW+P P + HP+I N
Sbjct: 231 EIWIPPPPYDPHPEIVN 247
>At3g26140.1 68416.m03261 glycosyl hydrolase family 5 protein /
cellulase family protein contains Pfam profile: PF00150
cellulase (glycosyl hydrolase family 5)
Length = 508
Score = 27.5 bits (58), Expect = 9.9
Identities = 16/71 (22%), Positives = 32/71 (45%)
Frame = +3
Query: 33 SSFSSTT*LVIILPVLDSFVKMAQVLKKLTTQVLKPNNVDTIVGCTGLRASSTWWNNVQM 212
S F + +I LP+++++ K+ L V+ N+V C G + ++ +
Sbjct: 93 SGFETKNPSMIDLPLIEAYKKVVAKLGNNNVMVILDNHVTKPGWCCGYNDGNGFFGDTFF 152
Query: 213 GPPDVILGITE 245
P I G+T+
Sbjct: 153 DPTTWIAGLTK 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,092,126
Number of Sequences: 28952
Number of extensions: 416471
Number of successful extensions: 1011
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 970
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1005
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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