BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20212 (699 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15) 99 4e-21 SB_45157| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.90 SB_21783| Best HMM Match : Keratin_B2 (HMM E-Value=0.81) 31 1.2 SB_14043| Best HMM Match : Extensin_1 (HMM E-Value=0.75) 30 2.1 SB_53582| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.1 SB_50787| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.6 SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35) 24 4.3 SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.8 SB_36342| Best HMM Match : Viral_helicase1 (HMM E-Value=1.1) 29 4.8 SB_26835| Best HMM Match : TGF_beta (HMM E-Value=7.3e-33) 29 4.8 SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13) 28 6.3 SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.3 SB_43823| Best HMM Match : MAM (HMM E-Value=0) 28 6.3 SB_6893| Best HMM Match : PPV_E2_C (HMM E-Value=0.94) 28 6.3 SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.3 SB_30225| Best HMM Match : Peptidase_S8 (HMM E-Value=0.0034) 25 9.2 >SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15) Length = 710 Score = 98.7 bits (235), Expect = 4e-21 Identities = 43/60 (71%), Positives = 51/60 (85%) Frame = +3 Query: 51 TTMGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKV 230 T ++ DT F +G+SGAS T+P QCS+LRKNG V++KGRPCKIVEMSTSKTGKHGHAKV Sbjct: 585 TMAEELADTEFHSGESGASDTYPAQCSSLRKNGHVVIKGRPCKIVEMSTSKTGKHGHAKV 644 Score = 68.1 bits (159), Expect = 6e-12 Identities = 28/62 (45%), Positives = 43/62 (69%) Frame = +2 Query: 329 QLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEECVIA 508 ++T+I +DGYL LM DNGD R D+K+ D D+ ++R F++ + + TVLK+ GEE V+ Sbjct: 643 KVTNIEEDGYLELMDDNGDTRADIKLQDNDIAKEIRAKFEASENFMVTVLKAMGEETVVG 702 Query: 509 SK 514 K Sbjct: 703 VK 704 >SB_45157| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 2870 Score = 31.1 bits (67), Expect = 0.90 Identities = 15/42 (35%), Positives = 21/42 (50%), Gaps = 2/42 (4%) Frame = -1 Query: 528 ESC--VCFEAMTHSSPQDFSTVHNNSLPLSKSVRNCVPRSPS 409 +SC +CF + S P + H+N+ P S NC P PS Sbjct: 1285 QSCPKICFTSCKPSCPVHCCSEHSNACPQECSTDNCKPSCPS 1326 >SB_21783| Best HMM Match : Keratin_B2 (HMM E-Value=0.81) Length = 316 Score = 30.7 bits (66), Expect = 1.2 Identities = 21/72 (29%), Positives = 30/72 (41%), Gaps = 4/72 (5%) Frame = -2 Query: 629 KLNLYTILCLHLGKSCDCKN*KFVPYKC*VVYLSRAVFALRR*RTPHRRISARCT----T 462 ++ LY ++ G CD + V +C V Y R V+ R R + CT Sbjct: 107 RVALYRVVSCSYGLQCDVALYRVVYVRCTVSYFIRVVYVRRTVSYFLRGVYVLCTVSCRV 166 Query: 461 IPCRCRSQYATV 426 I CR R+ Y V Sbjct: 167 ISCRVRTAYRVV 178 >SB_14043| Best HMM Match : Extensin_1 (HMM E-Value=0.75) Length = 568 Score = 29.9 bits (64), Expect = 2.1 Identities = 14/46 (30%), Positives = 23/46 (50%), Gaps = 1/46 (2%) Frame = +1 Query: 64 TSKTHTLRPETPGPQPPSPCNVRPCVKTVSLC*RVVH-ARLLKCPH 198 T+K HT +P T P P N+ P + + +L ++H + PH Sbjct: 168 TTKPHTTKPHTTKPHTTKPHNIDPTLPSPTLLNALLHFLYFYQAPH 213 Score = 29.5 bits (63), Expect = 2.7 Identities = 14/41 (34%), Positives = 20/41 (48%) Frame = +1 Query: 16 HISFLTVVKFKTQQWVTSKTHTLRPETPGPQPPSPCNVRPC 138 H L K KT + T+K +T +P T P+ P +PC Sbjct: 92 HTIKLYTTKPKTTKPHTNKPYTTKPRTTKPRTTKPHTTKPC 132 >SB_53582| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 393 Score = 29.9 bits (64), Expect = 2.1 Identities = 19/52 (36%), Positives = 25/52 (48%) Frame = +1 Query: 52 QQWVTSKTHTLRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKP 207 +Q V S P P P PP+PC + PC +T +VVH+ L P P Sbjct: 126 EQHVVSHVMHPAPPPPPPPPPAPC-MPPCHQT-----QVVHSVQLHASPPGP 171 >SB_50787| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 599 Score = 29.1 bits (62), Expect = 3.6 Identities = 16/58 (27%), Positives = 26/58 (44%) Frame = -2 Query: 308 GVRPCCVWRDRYLHTFYHEDINPNQVNFSVAVLSGFGCGHFNNLAWTTLQHNETVFTQ 135 G+R C D L F + + + + S A ++ +G GH N + L H + TQ Sbjct: 243 GLRSCITHGDDLLSVFDNMESSSKVFDMSNAPVTRYGLGHVNQILEEELSHLRFLKTQ 300 >SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35) Length = 458 Score = 23.8 bits (49), Expect(2) = 4.3 Identities = 8/13 (61%), Positives = 9/13 (69%) Frame = +1 Query: 97 PGPQPPSPCNVRP 135 PGPQ P P N+ P Sbjct: 362 PGPQDPGPGNILP 374 Score = 23.4 bits (48), Expect(2) = 4.3 Identities = 11/43 (25%), Positives = 21/43 (48%) Frame = +1 Query: 115 SPCNVRPCVKTVSLC*RVVHARLLKCPHPKPESTATLKFTWLG 243 +PC ++ C + +S+ V ++K P P TL ++G Sbjct: 398 NPCRIQYCTQEISMTPIHVLLLIVKAPILDPSLVVTLCSRFIG 440 >SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1438 Score = 28.7 bits (61), Expect = 4.8 Identities = 19/82 (23%), Positives = 33/82 (40%) Frame = +2 Query: 281 PSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKE 460 P + + V + +++ D + ADN + RE+ + +L ++ T G Sbjct: 284 PESDGIRVDETENGEHEAVDELPEDKPDTEADNYEQREETPTKEDELKSECTTSDSEGTP 343 Query: 461 LLCTVLKSCGEECVIASKQTQL 526 T KS GEE V + L Sbjct: 344 SAATYGKSDGEENVAQESEESL 365 >SB_36342| Best HMM Match : Viral_helicase1 (HMM E-Value=1.1) Length = 872 Score = 28.7 bits (61), Expect = 4.8 Identities = 27/95 (28%), Positives = 42/95 (44%), Gaps = 4/95 (4%) Frame = +2 Query: 272 DICPSTHNMDVPHVKREDYQLTDISDDGYLTLM----ADNGDLREDLKIPDGDLGTQLRT 439 D+ S H +D+P+ E++ + +D ++ +D L E L+IPD T + Sbjct: 592 DLNTSNH-IDLPYNSYENFDFDILEEDECMSEFRFHKSDIPLLAEMLQIPDRL--TLYQR 648 Query: 440 DFDSGKELLCTVLKSCGEECVIASKQTQLSTNKPL 544 SG E LC VLK C A + S P+ Sbjct: 649 SVCSGLEALCIVLKRLAYPCRYADMVAKFSRPVPV 683 >SB_26835| Best HMM Match : TGF_beta (HMM E-Value=7.3e-33) Length = 657 Score = 28.7 bits (61), Expect = 4.8 Identities = 18/55 (32%), Positives = 24/55 (43%), Gaps = 2/55 (3%) Frame = -2 Query: 353 HHQRYQSVGSLRASRGVRPCCVWRDRY--LHTFYHEDINPNQVNFSVAVLSGFGC 195 H + Q V LR R + PCC + +Y +D N Q N S V+ GC Sbjct: 602 HTEVIQKVALLRKQRELSPCCAPTKMFDLSLLYYDKDENLFQENVSNMVVEECGC 656 >SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13) Length = 492 Score = 28.3 bits (60), Expect = 6.3 Identities = 17/44 (38%), Positives = 22/44 (50%) Frame = -1 Query: 489 PQDFSTVHNNSLPLSKSVRNCVPRSPSGILRSSRRSPLSAIRVR 358 P+ S V + +LP+ V C+P SPS I RS P VR Sbjct: 253 PRQISNVRSLTLPVRYQVIGCLP-SPSDIKRSVPYPPRQISNVR 295 >SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 523 Score = 28.3 bits (60), Expect = 6.3 Identities = 23/82 (28%), Positives = 35/82 (42%), Gaps = 4/82 (4%) Frame = +2 Query: 296 MDVPHVKREDYQLTDISDDGYLT----LMADNGDLREDLKIPDGDLGTQLRTDFDSGKEL 463 +D+P+ K + + L ++DD L+ + D L E L PD + R D G E Sbjct: 37 LDIPYEKYDKFDLESLTDDECLSEFRFIKNDLYRLNEALNFPD-QITCPNRLTVD-GMEA 94 Query: 464 LCTVLKSCGEECVIASKQTQLS 529 LC L+ C Q+S Sbjct: 95 LCMTLRRFAYPCRYEDLVPQIS 116 >SB_43823| Best HMM Match : MAM (HMM E-Value=0) Length = 1724 Score = 28.3 bits (60), Expect = 6.3 Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 2/28 (7%) Frame = -1 Query: 153 RNRFYAGPNIAW--GRWLRPRSLRSQSV 76 R F+ PN+A G+WL P+SLR+ V Sbjct: 947 REGFFNNPNLAGCKGQWLGPKSLRASRV 974 >SB_6893| Best HMM Match : PPV_E2_C (HMM E-Value=0.94) Length = 1058 Score = 28.3 bits (60), Expect = 6.3 Identities = 14/35 (40%), Positives = 17/35 (48%) Frame = +1 Query: 31 TVVKFKTQQWVTSKTHTLRPETPGPQPPSPCNVRP 135 +VV + VT K T +P TP P P P RP Sbjct: 758 SVVAMPAARPVTPKPVTPKPVTPKPVTPKPVTTRP 792 >SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 154 Score = 28.3 bits (60), Expect = 6.3 Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Frame = +3 Query: 141 KNGFVMLKGRPCKIV-EMSTSKTGKHGHAKVHLVG 242 + G +M +G+PCKI + K G HG +H+ G Sbjct: 17 RRGVMMAEGKPCKITGTIEGLKAGNHGF-HIHVYG 50 >SB_30225| Best HMM Match : Peptidase_S8 (HMM E-Value=0.0034) Length = 605 Score = 25.0 bits (52), Expect(2) = 9.2 Identities = 9/17 (52%), Positives = 10/17 (58%) Frame = +1 Query: 70 KTHTLRPETPGPQPPSP 120 K H +P P P PPSP Sbjct: 344 KEHPDKPRDPAPVPPSP 360 Score = 21.0 bits (42), Expect(2) = 9.2 Identities = 8/18 (44%), Positives = 10/18 (55%) Frame = +1 Query: 94 TPGPQPPSPCNVRPCVKT 147 T GP PP+ N +P T Sbjct: 377 TFGPPPPAASNAKPAQYT 394 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 23,705,400 Number of Sequences: 59808 Number of extensions: 532474 Number of successful extensions: 1534 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 1372 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1525 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1829596184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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