Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20212
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 104 7e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 101 3e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 101 5e-22
At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 2.2
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 3.0
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.9
At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,... 29 3.9
At3g51330.1 68416.m05619 aspartyl protease family protein contai... 29 3.9
At3g51290.1 68416.m05614 proline-rich family protein 28 5.2
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 9.0
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 9.0
At2g28550.1 68415.m03468 AP2 domain-containing transcription fac... 27 9.0
At1g35830.1 68414.m04452 VQ motif-containing protein contains PF... 27 9.0
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 104 bits (249), Expect = 7e-23
Identities = 43/72 (59%), Positives = 56/72 (77%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
Score = 104 bits (249), Expect = 7e-23
Identities = 47/86 (54%), Positives = 65/86 (75%), Gaps = 1/86 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 436
KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q++
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIASK 514
+ FD GK+L+ +V+ + GEE + A K
Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 101 bits (243), Expect = 3e-22
Identities = 46/87 (52%), Positives = 65/87 (74%), Gaps = 1/87 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIASKQ 517
F+ GK+++ +V+ + GEE + A K+
Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALKE 154
Score = 100 bits (240), Expect = 8e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 101 bits (242), Expect = 5e-22
Identities = 45/87 (51%), Positives = 64/87 (73%), Gaps = 1/87 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIASKQ 517
FD GK+++ +V+ S GEE + A K+
Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVKE 154
Score = 96.3 bits (229), Expect = 2e-20
Identities = 40/72 (55%), Positives = 52/72 (72%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 322
Score = 29.5 bits (63), Expect = 2.2
Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = -3
Query: 478 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVGY 302
+HGA + AA++V L +RD E + V+V S + + + D++ ++V G
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232
Query: 301 VHVVCGGTD 275
+ CGG D
Sbjct: 233 RELSCGGDD 241
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +2
Query: 398 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 499
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -3
Query: 169 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 62
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,
putative strong similarity to SP|P54151 Peptide
methionine sulfoxide reductase (EC 1.8.4.6) {Brassica
napus}; contains Pfam profile PF01625: Peptide
methionine sulfoxide reductase
Length = 258
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = +2
Query: 251 LHGKKYEDICPST--HNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLG 424
+H YED+C T HN V R Y + S + L + + D L GD+G
Sbjct: 126 VHNPSYEDVCTGTTGHN----EVVRVQYDPKECSFESLLDVFWNRHD-PTTLNRQGGDVG 180
Query: 425 TQLRT 439
TQ R+
Sbjct: 181 TQYRS 185
>At3g51330.1 68416.m05619 aspartyl protease family protein contains
Eukaryotic and viral aspartyl proteases active site,
PROSITE:PS00141
Length = 529
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/51 (29%), Positives = 24/51 (47%)
Frame = +2
Query: 413 GDLGTQLRTDFDSGKELLCTVLKSCGEECVIASKQTQLSTNKPLSIYRVQT 565
G T D+G +L +CG C+ K+ LS ++PL++Y T
Sbjct: 109 GTPATWFLVALDTGSDLFWLPC-NCGSTCIRDLKEVGLSQSRPLNLYSPNT 158
>At3g51290.1 68416.m05614 proline-rich family protein
Length = 602
Score = 28.3 bits (60), Expect = 5.2
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +1
Query: 46 KTQQWVTSKTHTLRPETPGPQPPSP 120
+T W T+ T ++ P P P PP P
Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.5 bits (58), Expect = 9.0
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 311 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 460
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +2
Query: 341 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 475
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At2g28550.1 68415.m03468 AP2 domain-containing transcription factor
RAP2.7 (RAP2.7) nearly identical to AP2 domain
transcription factor RAP2.7 (GI:2281639) [Arabidopsis
thaliana]
Length = 449
Score = 27.5 bits (58), Expect = 9.0
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +1
Query: 70 KTHTLRPETPGPQPP 114
+T +RP+ PGPQPP
Sbjct: 402 QTSAVRPQPPGPQPP 416
>At1g35830.1 68414.m04452 VQ motif-containing protein contains
PF05678: VQ motif
Length = 302
Score = 27.5 bits (58), Expect = 9.0
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +1
Query: 22 SFLTVVKFKTQQWVTSKTHTLRPETPGPQPPSP 120
++L V + ++TH L P+ P P PP P
Sbjct: 60 TYLNFVNNLISDDILNQTHLLPPQPPPPPPPPP 92
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,983,656
Number of Sequences: 28952
Number of extensions: 354343
Number of successful extensions: 1064
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 991
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1053
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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