Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20195
(719 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 279 1e-75
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 279 1e-75
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 279 1e-75
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 279 1e-75
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 166 1e-41
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 161 6e-40
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 95 3e-20
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 87 8e-18
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 58 8e-09
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 58 8e-09
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 49 3e-06
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 49 4e-06
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 47 1e-05
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 45 4e-05
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.003
At5g13650.2 68418.m01585 elongation factor family protein contai... 38 0.009
At5g13650.1 68418.m01584 elongation factor family protein contai... 38 0.009
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 36 0.020
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 36 0.020
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 36 0.027
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 35 0.062
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 35 0.062
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 35 0.062
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.25
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.8
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.8
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.8
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 29 2.3
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.3
At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containi... 28 5.4
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.4
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 5.4
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 7.2
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 9.5
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 9.5
At3g21960.1 68416.m02770 receptor-like protein kinase-related co... 27 9.5
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 279 bits (684), Expect = 1e-75
Identities = 133/154 (86%), Positives = 140/154 (90%)
Frame = +2
Query: 50 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409
DKLKAERE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
G FEAGISK+GQTREHALLAFTLGVKQ+I NK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNK 154
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 687
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 279 bits (684), Expect = 1e-75
Identities = 133/154 (86%), Positives = 140/154 (90%)
Frame = +2
Query: 50 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409
DKLKAERE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
G FEAGISK+GQTREHALLAFTLGVKQ+I NK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNK 154
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 687
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 279 bits (684), Expect = 1e-75
Identities = 133/154 (86%), Positives = 140/154 (90%)
Frame = +2
Query: 50 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409
DKLKAERE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
G FEAGISK+GQTREHALLAFTLGVKQ+I NK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNK 154
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 687
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 279 bits (684), Expect = 1e-75
Identities = 133/154 (86%), Positives = 140/154 (90%)
Frame = +2
Query: 50 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409
DKLKAERE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
G FEAGISK+GQTREHALLAFTLGVKQ+I NK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNK 154
Score = 90.2 bits (214), Expect = 1e-18
Identities = 37/60 (61%), Positives = 49/60 (81%)
Frame = +1
Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 687
+MD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG
Sbjct: 154 KMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 166 bits (404), Expect = 1e-41
Identities = 73/150 (48%), Positives = 107/150 (71%), Gaps = 1/150 (0%)
Frame = +2
Query: 65 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 244
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 245 ERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 424
ERE GIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 425 GISK-NGQTREHALLAFTLGVKQLIVGVNK 511
G GQTREHA + GV+Q+IV +NK
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINK 387
Score = 34.7 bits (76), Expect = 0.062
Identities = 15/55 (27%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Frame = +1
Query: 532 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EPSTK--MPWFKG 687
YS+ RF+ IK+ V S+++ + +++ ++P+S N++ PS W++G
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 161 bits (390), Expect = 6e-40
Identities = 74/151 (49%), Positives = 105/151 (69%)
Frame = +2
Query: 59 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 238
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 239 KAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 418
+ ER G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 419 EAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
E G + GQTREH LA TLGV +LIV VNK
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNK 248
Score = 41.5 bits (93), Expect = 5e-04
Identities = 22/63 (34%), Positives = 37/63 (58%), Gaps = 3/63 (4%)
Frame = +1
Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMLEPSTK--MPW 678
+MD +S+ R++EI++++ ++K GYN V F+PISG G NM + + PW
Sbjct: 248 KMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEICPW 307
Query: 679 FKG 687
+ G
Sbjct: 308 WSG 310
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 95.5 bits (227), Expect = 3e-20
Identities = 56/155 (36%), Positives = 81/155 (52%)
Frame = +2
Query: 47 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 226
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 227 LDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 406
+D ER GITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 407 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
G QT+EH LLA +GV ++V +NK
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNK 204
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 87.4 bits (207), Expect = 8e-18
Identities = 55/152 (36%), Positives = 78/152 (51%)
Frame = +2
Query: 56 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 235
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 236 LKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 415
E++ GITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 416 FEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
QT+EH LLA +GV L+ +NK
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNK 192
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 57.6 bits (133), Expect = 8e-09
Identities = 46/151 (30%), Positives = 68/151 (45%)
Frame = +2
Query: 74 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 253
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 254 LGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 433
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 434 KNGQTREHALLAFTLGVKQLIVGVNKWIPLN 526
+ Q R + + K +G + W LN
Sbjct: 177 VDRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 57.6 bits (133), Expect = 8e-09
Identities = 46/151 (30%), Positives = 68/151 (45%)
Frame = +2
Query: 74 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 253
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 254 LGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 433
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 434 KNGQTREHALLAFTLGVKQLIVGVNKWIPLN 526
+ Q R + + K +G + W LN
Sbjct: 177 VDRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 49.2 bits (112), Expect = 3e-06
Identities = 35/113 (30%), Positives = 52/113 (46%)
Frame = +2
Query: 74 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 253
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 254 LGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 48.8 bits (111), Expect = 4e-06
Identities = 35/117 (29%), Positives = 55/117 (47%)
Frame = +2
Query: 62 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 241
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 242 AERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
E+E GITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 47.2 bits (107), Expect = 1e-05
Identities = 35/117 (29%), Positives = 53/117 (45%), Gaps = 4/117 (3%)
Frame = +2
Query: 74 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 253
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 254 LGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
GITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 45.2 bits (102), Expect = 4e-05
Identities = 42/132 (31%), Positives = 55/132 (41%), Gaps = 7/132 (5%)
Frame = +2
Query: 38 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 217
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 218 AWVLDKLKAERELGITIDIALWKFET-------SKYYVTIIDAPGHRDFIKNMITGTSQA 376
LDKL+ ER + + A +E S Y + +ID PGH DF + S
Sbjct: 103 ---LDKLQRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSAC 159
Query: 377 DCAVLIVAAGTG 412
A+L+V A G
Sbjct: 160 QGALLVVDAAQG 171
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.1 bits (87), Expect = 0.003
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +2
Query: 74 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 241
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 242 AERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 397
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 37.5 bits (83), Expect = 0.009
Identities = 32/113 (28%), Positives = 48/113 (42%)
Frame = +2
Query: 74 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 253
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 254 LGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
GITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 182
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 37.5 bits (83), Expect = 0.009
Identities = 32/113 (28%), Positives = 48/113 (42%)
Frame = +2
Query: 74 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 253
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 254 LGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
GITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 181
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 36.3 bits (80), Expect = 0.020
Identities = 47/190 (24%), Positives = 84/190 (44%), Gaps = 14/190 (7%)
Frame = +2
Query: 44 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 214
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 215 YAWVLDKLK---------AERELGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 361
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 362 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVS 541
G + D A+L++AA QT EH + +K +I+ NK I L + +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNK-IDLINEKAA 199
Query: 542 PDLRKSRRKY 571
+ ++ +K+
Sbjct: 200 TEQHEAIQKF 209
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 36.3 bits (80), Expect = 0.020
Identities = 47/190 (24%), Positives = 84/190 (44%), Gaps = 14/190 (7%)
Frame = +2
Query: 44 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 214
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 215 YAWVLDKLK---------AERELGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 361
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 362 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVS 541
G + D A+L++AA QT EH + +K +I+ NK I L + +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNK-IDLINEKAA 199
Query: 542 PDLRKSRRKY 571
+ ++ +K+
Sbjct: 200 TEQHEAIQKF 209
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 35.9 bits (79), Expect = 0.027
Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 4/99 (4%)
Frame = +2
Query: 287 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 454
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 455 HALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKY 571
H + +K +I+ NK I L V+ + ++ +K+
Sbjct: 166 HLAAVEIMQLKHIIILQNK-IDLIQENVAINQHEAIQKF 203
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 34.7 bits (76), Expect = 0.062
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = +2
Query: 305 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 484
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 485 KQLIVGVNK 511
K +I+ NK
Sbjct: 174 KDIIIIQNK 182
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.062
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +2
Query: 260 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.062
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +2
Query: 260 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 314 IIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 314 IIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 314 IIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 29.5 bits (63), Expect = 2.3
Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
Frame = +1
Query: 478 RCQTAHRRSKQMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG--DNM 651
R T+H + K ++STE E KEV ++K +GY+ + V ++ +G D +
Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYGGDDRL 371
Query: 652 LEPSTKMP 675
E ++ P
Sbjct: 372 SELKSRFP 379
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.3
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2
Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 397
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 747
Score = 28.3 bits (60), Expect = 5.4
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +3
Query: 588 DWLQPSCCRFRAHFWMARRQHVGAFNQNALVQGM 689
DW+ C AHF RR +GAF A G+
Sbjct: 687 DWVNCGSCGEWAHFGCDRRPGLGAFKDYAKTDGL 720
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.4
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +1
Query: 535 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 624
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 28.3 bits (60), Expect = 5.4
Identities = 19/64 (29%), Positives = 25/64 (39%), Gaps = 4/64 (6%)
Frame = +2
Query: 233 KLKAERELGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 400
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 401 AGTG 412
A G
Sbjct: 585 ADDG 588
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 541 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 651
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +1
Query: 517 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 630
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +1
Query: 517 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 630
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At3g21960.1 68416.m02770 receptor-like protein kinase-related
contains Pfam profile: PF01657 Domain of unknown
function that is usually associated with protein kinase
domain Pfam:PF00069; belongs to a family which includes
receptor-like kinase family proteins
Length = 262
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = -3
Query: 321 SMMVT*YLLVSNFQRAISIVIPSSRSAFSLSNTQAYL 211
S VT +L+ ++ +++ +PS S SL+ T AYL
Sbjct: 4 SSSVTKHLISASILAIVAMQLPSVHSVLSLNETNAYL 40
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,357,345
Number of Sequences: 28952
Number of extensions: 350819
Number of successful extensions: 1139
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1083
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1130
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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