Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20188
(730 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 2.1
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 3.6
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 3.6
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 3.6
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 26 4.8
SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 26 6.3
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 25 8.4
SPBP4G3.02 |pho1||acid phosphatase Pho1 |Schizosaccharomyces pom... 25 8.4
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 8.4
SPBC23E6.09 |ssn6||transcriptional corepressor Ssn6|Schizosaccha... 25 8.4
SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity f... 25 8.4
SPAC17A5.02c |dbr1||RNA lariat debranching enzyme Dbr1 |Schizosa... 25 8.4
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 25 8.4
SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S... 25 8.4
>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1184
Score = 27.5 bits (58), Expect = 2.1
Identities = 21/69 (30%), Positives = 28/69 (40%)
Frame = -1
Query: 298 DGAAEVLGRERPER*YSHFWMSRALQSFKSTKPKILSSASLAQTLSPSCVGGPPTNAPTS 119
D A E+L R P+ + W R FK+ K K L + S G APTS
Sbjct: 1005 DNAKEILSRSSPKSPVA--WFKR---KFKNRKDKAAVKRMLEEDSSKQLSSGRDVVAPTS 1059
Query: 118 SSKSISLQG 92
+ +S G
Sbjct: 1060 VNHDVSHVG 1068
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 26.6 bits (56), Expect = 3.6
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = +2
Query: 530 LQPQTGRLARLGHHQWGGGGLVWEHVGTVLEGTKPVRLSTGEE 658
L + G R +WGG WE V LE +P+ + GEE
Sbjct: 706 LSREAGEKFRKAILRWGGSRSPWECVAEALE--QPILATGGEE 746
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 26.6 bits (56), Expect = 3.6
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = -1
Query: 235 SRALQSFKSTKPKILSSASLAQTLSPSCVGGPPTNAPTSSSKSIS 101
S +L S + + SS+SLA + + S PT++ +SS + S
Sbjct: 72 SSSLTSSSAASSSLTSSSSLASSSTNSTTSASPTSSSLTSSSATS 116
>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 949
Score = 26.6 bits (56), Expect = 3.6
Identities = 14/34 (41%), Positives = 17/34 (50%)
Frame = +1
Query: 286 RQHHLAVDSDLRGTASVHRRQLPSGPDSVPLLET 387
R HH+ V D T S ++ L SV LLET
Sbjct: 689 RVHHVKVSEDCTATVSFGKQSLHIVRSSVTLLET 722
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 26.2 bits (55), Expect = 4.8
Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = -3
Query: 620 PGQFQHAPKRVRRLPTDGARAEQVS--RSEVAARDSLCAIAVSPSNTCTDSSNGKRWSPP 447
P ++ + +RR T G +E S RS DS+ + + S S++ + SN K +S
Sbjct: 58 PTPISYSFRSLRRGHTSGHASEHTSSSRSSHDESDSMSSSSESDSDSVSSESNPKSYSDS 117
Query: 446 GT 441
T
Sbjct: 118 ST 119
>SPAC227.15 |||protein phosphatase regulatory subunit Reg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 873
Score = 25.8 bits (54), Expect = 6.3
Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = +1
Query: 145 PPHNWGRGSAPRTPKTK-FLVSCF*--TTGVHVTSRSGSTTSRAVHGQEPRQHHLAVDSD 315
PP GR S +P+T F S ++ VH ++ TTS +V + P ++H + S
Sbjct: 691 PPPYMGRQSLNDSPQTSDFKASNLNDSSSNVHSIFQTRETTSPSVQNKTPTKYHRELKSS 750
Query: 316 LRG 324
G
Sbjct: 751 KDG 753
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 8.4
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -2
Query: 696 AQRDVSPSCMNFLSSPV 646
++ D +PS MNFL+SPV
Sbjct: 482 SRNDGNPSTMNFLTSPV 498
>SPBP4G3.02 |pho1||acid phosphatase Pho1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 453
Score = 25.4 bits (53), Expect = 8.4
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +2
Query: 308 IVTLEALRPFIVDNFPQDPTPFPYLRH 388
I+ +E F DN P++P P Y H
Sbjct: 334 IIPVETALGFFTDNTPENPLPTSYQVH 360
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.4 bits (53), Expect = 8.4
Identities = 19/43 (44%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = -1
Query: 190 SSASLAQTLSPSCVGGPPTNAPTSS-SKSISLQGPKWGAAPST 65
SSA +Q S S V P +AP S S S Q P AAP+T
Sbjct: 140 SSAPASQEPSASGVNAPTVSAPNSMVSPPPSFQPPS-AAAPAT 181
>SPBC23E6.09 |ssn6||transcriptional corepressor
Ssn6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1102
Score = 25.4 bits (53), Expect = 8.4
Identities = 24/87 (27%), Positives = 38/87 (43%), Gaps = 2/87 (2%)
Frame = -1
Query: 304 RRDGAAEVLGRERPER*YSHFWMSRALQSFKSTKPKILSSASLAQTLSPSCV--GGPPTN 131
R + + V RER S +R S +S PK + ++ L S V G P ++
Sbjct: 841 RNNSISNVSHRERSNSVSSKSRETRTSASNESD-PKKSTQRDSSKKLENSTVVSGSPSSS 899
Query: 130 APTSSSKSISLQGPKWGAAPSTGKVCP 50
+ + ++KSI Q P+ P G P
Sbjct: 900 SKSDAAKSIKPQKPEPALKPVEGTADP 926
>SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity
factor complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 25.4 bits (53), Expect = 8.4
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = -1
Query: 214 KSTKPKILSSASLAQTLSPSCVGGPPTNAPTSSSKSIS 101
K++ KIL S +L Q+ P CV PT + SIS
Sbjct: 119 KTSVLKILESYALKQSRHPICVNLDPTQPMLALPGSIS 156
>SPAC17A5.02c |dbr1||RNA lariat debranching enzyme Dbr1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 466
Score = 25.4 bits (53), Expect = 8.4
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = +2
Query: 299 SPWIVTLEALRPFIVDNFPQDPTPFPYL 382
S W+ L A+ PF QDP P P L
Sbjct: 369 SEWLSVLRAMHPFQSHTIEQDP-PLPSL 395
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 25.4 bits (53), Expect = 8.4
Identities = 13/40 (32%), Positives = 22/40 (55%)
Frame = -1
Query: 205 KPKILSSASLAQTLSPSCVGGPPTNAPTSSSKSISLQGPK 86
K + L+ + T +P+ V PP++A SSSK + P+
Sbjct: 610 KDRSLNVTPSSSTPTPASVLAPPSSASLSSSKDANRSVPE 649
>SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein
Vps33|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 25.4 bits (53), Expect = 8.4
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = -1
Query: 730 TYSESVALSSGRAT*RVSVLYEFSLLTSRKANGLGAF 620
TY E ++ G+ +S LY+ LL R + + F
Sbjct: 191 TYQEEATINFGKVEGEISALYDSVLLVDRSLDRITPF 227
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,415,041
Number of Sequences: 5004
Number of extensions: 80424
Number of successful extensions: 282
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 240
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 281
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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