SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20184
         (765 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    48   9e-06
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    45   5e-05
At2g24610.1 68415.m02940 cyclic nucleotide-regulated ion channel...    33   0.28 
At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S), ...    31   1.1  
At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase fa...    29   3.4  
At1g17360.1 68414.m02116 COP1-interacting protein-related simila...    29   3.4  
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g...    29   4.5  
At1g79280.1 68414.m09242 expressed protein weak similarity to Nu...    28   5.9  
At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein si...    28   7.8  
At2g17010.1 68415.m01961 mechanosensitive ion channel domain-con...    28   7.8  
At1g19130.1 68414.m02379 expressed protein                             28   7.8  

>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 47.6 bits (108), Expect = 9e-06
 Identities = 29/61 (47%), Positives = 32/61 (52%)
 Frame = +1

Query: 100 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKY*TRPDQNS 279
           MS K EKP L G RIKTRKR+     DP  F DA+VQ      GDL+   K     D N 
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIESSDLNF 59

Query: 280 T 282
           T
Sbjct: 60  T 60


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 45.2 bits (102), Expect = 5e-05
 Identities = 26/47 (55%), Positives = 28/47 (59%)
 Frame = +1

Query: 100 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLD 240
           MS K EKP L G RIKTRKR+     DP  F DALVQ      GDL+
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLE 46


>At2g24610.1 68415.m02940 cyclic nucleotide-regulated ion channel,
           putative (CNGC14) similar to cyclic nucleotide and
           calmodulin-regulated ion channel (GI:4581205)
           [Arabidopsis thaliana]
          Length = 726

 Score = 32.7 bits (71), Expect = 0.28
 Identities = 15/53 (28%), Positives = 27/53 (50%)
 Frame = -1

Query: 618 VHQDQQHGWRHASVTDPQCSHARQLDALFGIEAFQVDRTFFISSSNIFSKYLY 460
           +H+D ++ W++ +V    C  +  +   FGI A  + +   + SS    KYLY
Sbjct: 302 LHRDDRNNWQNTTVVFSNCDPSNNIQFTFGIFADALTKN--VVSSPFLEKYLY 352


>At5g07950.1 68418.m00922 expressed protein ferredoxin (2Fe-2S),
           Arabidopsis thaliana, PIR:B71412
          Length = 303

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 18/45 (40%), Positives = 28/45 (62%)
 Frame = -3

Query: 688 APA**SRTLPARSPSPGCPSADVRSPGPAARMEARIRYRSTVQSC 554
           +PA  SR+L + SPSPG  S+ +R   PAA+   R ++ S + +C
Sbjct: 13  SPASSSRSLASSSPSPGSFSSSMRLWRPAAQRNLRNQW-SNLSNC 56


>At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase
           family protein similar to SP|P31384 Glucose-repressible
           alcohol dehydrogenase transcriptional effector (Carbon
           catabolite repressor protein 4) {Saccharomyces
           cerevisiae}; contains Pfam profile PF03372:
           Endonuclease/Exonuclease/phosphatase family
          Length = 602

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 22/68 (32%), Positives = 29/68 (42%)
 Frame = -3

Query: 253 TCRLRRDRHRPAPDPVPERRGNRSGHTSLSHLFFWS*SVDPIILVFLPSDSYNILILTGY 74
           +C +   R  PAP P P R  + SG     HL     +  P+ +      SYNIL  T  
Sbjct: 205 SCTILTSRVIPAPSPSPRRLISISGTDVTGHL---DSNGRPLSMGTFTVLSYNILSDTYA 261

Query: 73  VGAFYSFC 50
               YS+C
Sbjct: 262 SSDIYSYC 269


>At1g17360.1 68414.m02116 COP1-interacting protein-related similar
           to COP1-Interacting Protein 7 (CIP7) (GI:3327870)
           [Arabidopsis thaliana]
          Length = 1032

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 19/70 (27%), Positives = 31/70 (44%)
 Frame = +3

Query: 525 RSRTAHQAGAHDCTVDR*RMRASIRAAGPGERTSAEGQPGDGLRAGSVRDHQAGARRHSL 704
           RSR+AH AG  D   D  + +   +   P ++  A   P   + AGS +  ++      L
Sbjct: 404 RSRSAHIAGGIDIKSDERQSKGRKKELFPSDKKQALTSPPKPVSAGSEQRQKSFGVEDDL 463

Query: 705 VTALKKGQLE 734
           V A   G+ +
Sbjct: 464 VNADAAGKFD 473


>At4g25000.1 68417.m03587 alpha-amylase, putative /
           1,4-alpha-D-glucan glucanohydrolase, putative similar to
           alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
           GI:21670851; contains Pfam profile PF00128: Alpha
           amylase, catalytic domain
          Length = 423

 Score = 28.7 bits (61), Expect = 4.5
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +1

Query: 160 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY*TR 264
           D K  YD N  R  L Q +E AGG +  A+ + T+
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTK 272


>At1g79280.1 68414.m09242 expressed protein weak similarity to
            Nucleoprotein TPR (Swiss-Prot:P12270) [Homo sapiens]
          Length = 2111

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 15/38 (39%), Positives = 22/38 (57%)
 Frame = -3

Query: 661  PARSPSPGCPSADVRSPGPAARMEARIRYRSTVQSCAP 548
            P R+PSPG  S+ + +    A+M+ R R  + V S AP
Sbjct: 2035 PKRAPSPGGGSSTIVTLADRAQMKRRERIANIVVSRAP 2072


>At5g41940.1 68418.m05106 RabGAP/TBC domain-containing protein
           similar to GTPase activating protein [Yarrowia
           lipolytica] GI:2370595; contains Pfam profile PF00566:
           TBC domain
          Length = 549

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 13/40 (32%), Positives = 19/40 (47%)
 Frame = +2

Query: 167 KRSMTRTVSATLWYRVWSGPVAISTQPTSTRLGRIKTRLP 286
           +R +T   +  LW  +W+   AI T       GRI+ R P
Sbjct: 448 RRELTFEQTLCLWEVMWADQAAIRTGIAKATWGRIRLRAP 487


>At2g17010.1 68415.m01961 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 779

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 18/71 (25%), Positives = 27/71 (38%)
 Frame = +2

Query: 227 VAISTQPTSTRLGRIKTRLPTLWRSHIDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANE 406
           V+  T PT T   R         R   +V  AGG  + G   S    S  +++     N+
Sbjct: 154 VSHQTMPTPTSTARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQ 213

Query: 407 DMDTMRNFEQV 439
           D   ++  E V
Sbjct: 214 DQPQLQEEEVV 224


>At1g19130.1 68414.m02379 expressed protein
          Length = 187

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 18/70 (25%), Positives = 32/70 (45%)
 Frame = +2

Query: 275 TRLPTLWRSHIDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLM 454
           ++LP  ++    V  +   LLP GSVS     P   T  F   E +  +R+     ++  
Sbjct: 40  SQLPPTFKVDRAVSTSIYFLLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTS 99

Query: 455 RRYKYLEKMF 484
              ++L++MF
Sbjct: 100 GLVRFLQRMF 109


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,525,120
Number of Sequences: 28952
Number of extensions: 366356
Number of successful extensions: 1324
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1323
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -