SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20179
         (762 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   158   9e-40
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   151   1e-37
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...   128   1e-30
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   125   8e-30
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...    95   1e-20
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    50   4e-07
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    44   3e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    29   0.55 
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar...    29   0.55 
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar...    29   0.96 
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual    27   2.9  
SPAC22F8.09 |rrp16|nop53|rRNA processing protein Rrp16 |Schizosa...    26   6.7  
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    25   8.9  

>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  158 bits (383), Expect = 9e-40
 Identities = 86/170 (50%), Positives = 108/170 (63%), Gaps = 2/170 (1%)
 Frame = +2

Query: 254 VNPNNTIFDAKRLIGRKFEDATVQADMKHWPIEVVSDGGKPKIKVAYKGEDKTFFPEEVS 433
           +NP+NTIFDAKRLIGRKF+D  VQ+DMKHWP +V+S  GKP ++V YKGE KTF PEE+S
Sbjct: 59  MNPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEIS 118

Query: 434 SMVLTKMKETAEAYLGKTVQNAVITFPRTSMTLKDKPQKMQVPSLA*TFSESSMNXXXXX 613
           SMVL KM+ETAEAYLG  V +AV+T P      + +  K     +A       +N     
Sbjct: 119 SMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATK-DAGLIAGLNVLRIINEPTAA 177

Query: 614 XXXXXXXXXXENEMYLSL--TRRRYFDVSILTIEDGIFEVKSTAGDTHFG 757
                     + E  + +       FDVS+LTIE+GIFEVK+TAGDTH G
Sbjct: 178 AIAYGLDRSNQGESNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLG 227



 Score =  106 bits (254), Expect = 4e-24
 Identities = 49/58 (84%), Positives = 53/58 (91%)
 Frame = +3

Query: 90  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA*TP 263
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA  P
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61



 Score = 99.5 bits (237), Expect = 5e-22
 Identities = 47/54 (87%), Positives = 49/54 (90%)
 Frame = +1

Query: 508 VPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFD 669
           VPAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+   GE NVLIFD
Sbjct: 144 VPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFD 197


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  151 bits (365), Expect = 1e-37
 Identities = 82/170 (48%), Positives = 107/170 (62%), Gaps = 2/170 (1%)
 Frame = +2

Query: 254 VNPNNTIFDAKRLIGRKFEDATVQADMKHWPIEVVSDGGKPKIKVAYKGEDKTFFPEEVS 433
           +NP+NTIFDAKRLIGR+F D  VQ+DMKHWP +V+   GKP I+V +KGE KTF PEE+S
Sbjct: 59  MNPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEIS 118

Query: 434 SMVLTKMKETAEAYLGKTVQNAVITFPRTSMTLKDKPQKMQVPSLA*TFSESSMNXXXXX 613
           SMVL KM+E+AEA+LG  V +AV+T P      + +  K     +A       +N     
Sbjct: 119 SMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATK-DAGLIAGLNVLRIINEPTAA 177

Query: 614 XXXXXXXXXXENEMYLSL--TRRRYFDVSILTIEDGIFEVKSTAGDTHFG 757
                     ++E  + +       FDVS+LTIE+GIFEVK+TAGDTH G
Sbjct: 178 AIAYGLDRSNQHETNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLG 227



 Score =  106 bits (254), Expect = 4e-24
 Identities = 49/58 (84%), Positives = 53/58 (91%)
 Frame = +3

Query: 90  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA*TP 263
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA  P
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61



 Score = 96.3 bits (229), Expect = 4e-21
 Identities = 46/54 (85%), Positives = 48/54 (88%)
 Frame = +1

Query: 508 VPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFD 669
           VPAYFNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+    E NVLIFD
Sbjct: 144 VPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFD 197


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score =  128 bits (308), Expect = 1e-30
 Identities = 72/170 (42%), Positives = 95/170 (55%), Gaps = 2/170 (1%)
 Frame = +2

Query: 254 VNPNNTIFDAKRLIGRKFEDATVQADMKHWPIEVVSDGGKPKIKVAYKGEDKTFFPEEVS 433
           +NP NT+FDAKRLIGR++ED   Q D+KHWP +V+ + G P I+V Y GE K F  +E+S
Sbjct: 63  MNPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEIS 122

Query: 434 SMVLTKMKETAEAYLGKTVQNAVITFPRTSMTLKDKPQK--MQVPSLA*TFSESSMNXXX 607
           +MVLTKMKE +EA L K V+ AVIT P      +    K    +  L      +      
Sbjct: 123 AMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAA 182

Query: 608 XXXXXXXXXXXXENEMYLSLTRRRYFDVSILTIEDGIFEVKSTAGDTHFG 757
                       +N +   L     FDVS+L I+ G+FEV +TAGDTH G
Sbjct: 183 IAYGLDAKSDKPKNVLIFDLGGGT-FDVSLLKIQGGVFEVLATAGDTHLG 231



 Score = 94.3 bits (224), Expect = 2e-20
 Identities = 43/58 (74%), Positives = 49/58 (84%)
 Frame = +3

Query: 90  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA*TP 263
           A+GIDLGTTYSCV V++   VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A  P
Sbjct: 8   AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65



 Score = 91.9 bits (218), Expect = 9e-20
 Identities = 44/54 (81%), Positives = 47/54 (87%)
 Frame = +1

Query: 508 VPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFD 669
           VPAYF+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K    +NVLIFD
Sbjct: 148 VPAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFD 201


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  125 bits (301), Expect = 8e-30
 Identities = 66/167 (39%), Positives = 95/167 (56%)
 Frame = +2

Query: 257 NPNNTIFDAKRLIGRKFEDATVQADMKHWPIEVVSDGGKPKIKVAYKGEDKTFFPEEVSS 436
           NP NTIFD KRLIGRKF++ T+  D+K +P  +V+D  +P ++V   G+ K F PEE+S+
Sbjct: 93  NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152

Query: 437 MVLTKMKETAEAYLGKTVQNAVITFPRTSMTLKDKPQKMQVPSLA*TFSESSMNXXXXXX 616
           M+L+KMK+TAEAYLGK V +AV+T P      + +  K                      
Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAI 212

Query: 617 XXXXXXXXXENEMYLSLTRRRYFDVSILTIEDGIFEVKSTAGDTHFG 757
                    E  + +       FDVS+L+I++G+FEV +T+GDTH G
Sbjct: 213 AYGLDKTDTEKHIVVYDLGGGTFDVSLLSIDNGVFEVLATSGDTHLG 259



 Score = 94.7 bits (225), Expect = 1e-20
 Identities = 42/57 (73%), Positives = 49/57 (85%)
 Frame = +3

Query: 93  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA*TP 263
           +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ    P
Sbjct: 38  IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNP 94



 Score = 91.5 bits (217), Expect = 1e-19
 Identities = 41/54 (75%), Positives = 51/54 (94%)
 Frame = +1

Query: 508 VPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFD 669
           VPAYFND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK  T E++++++D
Sbjct: 177 VPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYD 229


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score = 94.7 bits (225), Expect = 1e-20
 Identities = 56/168 (33%), Positives = 85/168 (50%)
 Frame = +2

Query: 254 VNPNNTIFDAKRLIGRKFEDATVQADMKHWPIEVVSDGGKPKIKVAYKGEDKTFFPEEVS 433
           +NP NT F  KRLIGR+F++  VQ D+K  P ++V            +   KT+ P ++ 
Sbjct: 108 INPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIG 164

Query: 434 SMVLTKMKETAEAYLGKTVQNAVITFPRTSMTLKDKPQKMQVPSLA*TFSESSMNXXXXX 613
             +L+KM+ETA  YLGK V+NAV+T P      + +  K    ++A       +N     
Sbjct: 165 GFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATK-AAGAIAGLNVLRVVNEPTAA 223

Query: 614 XXXXXXXXXXENEMYLSLTRRRYFDVSILTIEDGIFEVKSTAGDTHFG 757
                     +  + +       FD+SIL + +G+FEV+ST GDTH G
Sbjct: 224 ALAYGLDKKNDAIVAVFDLGGGTFDISILELNNGVFEVRSTNGDTHLG 271



 Score = 83.8 bits (198), Expect = 2e-17
 Identities = 40/54 (74%), Positives = 46/54 (85%)
 Frame = +1

Query: 508 VPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFD 669
           VPAYFNDSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK   +  V +FD
Sbjct: 190 VPAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFD 241



 Score = 68.9 bits (161), Expect = 7e-13
 Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
 Frame = +3

Query: 81  KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVA* 257
           K P +GIDLGTT SC+ + +    ++IAN +G RTTPS VAFT D ERL+G +AK Q   
Sbjct: 49  KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108

Query: 258 TPTTQY 275
            P   +
Sbjct: 109 NPENTF 114


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 50.0 bits (114), Expect = 4e-07
 Identities = 21/42 (50%), Positives = 31/42 (73%)
 Frame = +1

Query: 508 VPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDK 633
           +PA+F D QR+A  +A  I+GLN LR++N+  AAA+ YG+ K
Sbjct: 148 IPAWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITK 189



 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 20/59 (33%), Positives = 38/59 (64%)
 Frame = +3

Query: 78  AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 254
           ++   VGID G + + + V ++  +++I N+  NR+TPS V++ +  R +G+AAK+  A
Sbjct: 3   SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61



 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 26/87 (29%), Positives = 42/87 (48%), Gaps = 1/87 (1%)
 Frame = +2

Query: 257 NPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPIEVVSDGGKPKIKVAYKGEDKTFFPEEVS 433
           N  NT+   KRL GR ++D  ++  +      ++    G    KV Y  E+  F   ++ 
Sbjct: 63  NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122

Query: 434 SMVLTKMKETAEAYLGKTVQNAVITFP 514
           +   TK+K  AEA L  +V + VI+ P
Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISIP 149


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 21/66 (31%), Positives = 37/66 (56%)
 Frame = +1

Query: 496 CSYHVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDSA 675
           C   VP YF D+QR+A + A   +GL VL++I++P A  +A    ++   ++ V++ +  
Sbjct: 166 CVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILALMYSEEVLIDKTVVVANFG 225

Query: 676 AVLRRV 693
           A    V
Sbjct: 226 ATRSEV 231



 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 16/53 (30%), Positives = 30/53 (56%)
 Frame = +3

Query: 93  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQV 251
           +GI  G   S +   + GK +++AN++GNR  PS +++   +   G  A+ Q+
Sbjct: 27  IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQL 79


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 29.5 bits (63), Expect = 0.55
 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
 Frame = +3

Query: 93  VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA*TPTT 269
           + ID GT ++   + + G  +EI+      R   S VAF   ER+ G  A N     P  
Sbjct: 25  LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83

Query: 270 QYSMPNV 290
            +S+ NV
Sbjct: 84  -HSIRNV 89


>SPAC823.11 |||sphingosine-1-phosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 411

 Score = 29.5 bits (63), Expect = 0.55
 Identities = 17/57 (29%), Positives = 29/57 (50%)
 Frame = -1

Query: 171 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARLTLVP 1
           G W  SP S +  H   ++++  +F +RVL   C+      L  K  +K+A L ++P
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALLAVLP 327


>SPAC25B8.04c |||mitochondrial splicing suppressor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 378

 Score = 28.7 bits (61), Expect = 0.96
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +1

Query: 85  HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 180
           HP   S W+P    LVS+    W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314


>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1236

 Score = 27.1 bits (57), Expect = 2.9
 Identities = 31/141 (21%), Positives = 56/141 (39%)
 Frame = -1

Query: 726 TSKIPSSMVRMDTSKYRRRVKDKYISFSSTLFVKTVSNRSSSRFIDDSENVQARDGTCIF 547
           +S   SS+  + +      +   + S SS L   T S  S+S  I  S        +  F
Sbjct: 550 SSDFSSSITTISSGISSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSST------SSTF 603

Query: 546 CGLSLRVIEVRGNVITAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPLYATLILGLP 367
              S        + I++  T+L     + S + +S+  + S    +LSS +     + + 
Sbjct: 604 SSASTSSPSSISSSISSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSIST---IPIS 660

Query: 366 PSLTTSIGQCFMSACTVASSN 304
            SL+T       S+ T+ SS+
Sbjct: 661 SSLSTYSSSVIPSSSTLVSSS 681


>SPAC22F8.09 |rrp16|nop53|rRNA processing protein Rrp16
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 419

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 9/21 (42%), Positives = 15/21 (71%)
 Frame = -1

Query: 717 IPSSMVRMDTSKYRRRVKDKY 655
           +P S+  +  SKYR ++K+KY
Sbjct: 387 VPPSLPVLKKSKYRAKIKEKY 407


>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 570

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
 Frame = -2

Query: 263 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 114
           WGS    +G +   TLG+ + +      + L+VGD  LHL V ++ +A +R
Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,473,102
Number of Sequences: 5004
Number of extensions: 76580
Number of successful extensions: 275
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 246
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 274
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 365309308
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -