BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20174 (614 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosa... 109 2e-25 SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosac... 109 2e-25 SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy... 31 0.17 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 29 0.53 SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.71 SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 27 1.6 SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||... 27 2.2 SPBP23A10.12 |||FRG1 family protein|Schizosaccharomyces pombe|ch... 26 3.8 SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pomb... 26 3.8 SPCC757.07c |ctt1|cta1|catalase|Schizosaccharomyces pombe|chr 3|... 26 5.0 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 26 5.0 SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizos... 26 5.0 SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|c... 25 6.6 SPBC25H2.08c |mrs2||magnesium ion transporter Mrs2|Schizosacchar... 25 8.7 SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 25 8.7 SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1||... 25 8.7 >SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosaccharomyces pombe|chr 3|||Manual Length = 193 Score = 109 bits (263), Expect = 2e-25 Identities = 47/66 (71%), Positives = 61/66 (92%) Frame = +1 Query: 64 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 243 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQNIRK++KDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNIRKLVKDGLVIRKPNLMHSR 60 Query: 244 ARVRKT 261 R+RKT Sbjct: 61 FRIRKT 66 Score = 92.7 bits (220), Expect = 4e-20 Identities = 42/84 (50%), Positives = 54/84 (64%) Frame = +3 Query: 255 KNTEARRKGRHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLY 434 K A+R GRH G+GKR+GTA ARMP +W + KID+HLYH+LY Sbjct: 65 KTHAAKRLGRHTGYGKRKGTAEARMPSTVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLY 124 Query: 435 MKAKGNVFKNKRVLMEYIHRKKAE 506 ++AKGN FK+KR L+E+I R KAE Sbjct: 125 LEAKGNTFKHKRALIEHIQRAKAE 148 Score = 35.1 bits (77), Expect = 0.008 Identities = 18/42 (42%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = +2 Query: 485 HPQEEG*EA-RTKMLSDQAEARRNKVKEARNRRKERIAAKKE 607 H Q EA RTK++ +Q +ARR + K AR RR + + K+E Sbjct: 141 HIQRAKAEANRTKLIQEQQDARRARAKAARQRRAKAVEEKRE 182 >SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosaccharomyces pombe|chr 2|||Manual Length = 193 Score = 109 bits (263), Expect = 2e-25 Identities = 47/66 (71%), Positives = 61/66 (92%) Frame = +1 Query: 64 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 243 M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQN+RK+IKDGLVI+KP +HSR Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNVRKLIKDGLVIRKPNLMHSR 60 Query: 244 ARVRKT 261 R+RKT Sbjct: 61 FRIRKT 66 Score = 92.7 bits (220), Expect = 4e-20 Identities = 42/84 (50%), Positives = 54/84 (64%) Frame = +3 Query: 255 KNTEARRKGRHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLY 434 K A+R GRH G+GKR+GTA ARMP +W + KID+HLYH+LY Sbjct: 65 KTHAAKRLGRHTGYGKRKGTAEARMPSAVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLY 124 Query: 435 MKAKGNVFKNKRVLMEYIHRKKAE 506 ++AKGN FK+KR L+E+I R KAE Sbjct: 125 LEAKGNTFKHKRALIEHIQRAKAE 148 Score = 35.1 bits (77), Expect = 0.008 Identities = 18/42 (42%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = +2 Query: 485 HPQEEG*EA-RTKMLSDQAEARRNKVKEARNRRKERIAAKKE 607 H Q EA RTK++ +Q +ARR + K AR RR + + K+E Sbjct: 141 HIQRAKAEANRTKLIQEQQDARRARAKAARQRRAKAVEEKRE 182 >SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 30.7 bits (66), Expect = 0.17 Identities = 13/33 (39%), Positives = 23/33 (69%) Frame = +2 Query: 509 ARTKMLSDQAEARRNKVKEARNRRKERIAAKKE 607 AR + ++A +++ +VKE RN R+E+ +KKE Sbjct: 509 ARPAKIKNEAWSKQKEVKEKRNTRREKRKSKKE 541 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 29.1 bits (62), Expect = 0.53 Identities = 12/30 (40%), Positives = 21/30 (70%) Frame = -3 Query: 207 TILDHLTDVLSGVGVCDLIDFIWIQPHLLF 118 +I +H++DV G+ V LI+ + I+P +LF Sbjct: 351 SICEHVSDVSRGIAVETLIELMIIRPKILF 380 >SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 356 Score = 28.7 bits (61), Expect = 0.71 Identities = 12/32 (37%), Positives = 19/32 (59%) Frame = -3 Query: 186 DVLSGVGVCDLIDFIWIQPHLLFTTSHNRGCK 91 +VLS + ++D + PHLL +S N+ CK Sbjct: 237 EVLSKLPYFHILDKSFFSPHLLLQSSENQACK 268 >SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 27.5 bits (58), Expect = 1.6 Identities = 14/52 (26%), Positives = 24/52 (46%), Gaps = 1/52 (1%) Frame = -2 Query: 562 LHFIAAGLSLVAKHLRPGFSAFFLWMYSMSTRLFLNTLPFAFM-*SEWYRCL 410 ++ + L L +L AF LW+YS+ L+L + W++CL Sbjct: 317 VNILGDNLILSGIYLTSSRQAFVLWVYSLDKELWLQLDMLGVLNHGSWFKCL 368 >SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 600 Score = 27.1 bits (57), Expect = 2.2 Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 3/56 (5%) Frame = +1 Query: 34 LVCVGSH-RVRMSSLKLQKRLAASVMRCGKK--KVWLDPNEINEIANTNSRQNIRK 192 +V VG + ++ S + + K L S MRCG+K W++ + + NT+ + K Sbjct: 305 IVLVGKYTHLQDSYISVIKALEHSAMRCGRKLDLQWVEASHLEASTNTSDPLSYHK 360 >SPBP23A10.12 |||FRG1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 245 Score = 26.2 bits (55), Expect = 3.8 Identities = 11/38 (28%), Positives = 18/38 (47%) Frame = +1 Query: 46 GSHRVRMSSLKLQKRLAASVMRCGKKKVWLDPNEINEI 159 G +++ K K L +S+ W D NE+NE+ Sbjct: 9 GDKKIQKKKKKNTKSLQSSLESAENDPFWTDANELNEL 46 >SPAC2F3.06c |kap104||karyopherin Kap104|Schizosaccharomyces pombe|chr 1|||Manual Length = 910 Score = 26.2 bits (55), Expect = 3.8 Identities = 14/31 (45%), Positives = 18/31 (58%) Frame = -2 Query: 541 LSLVAKHLRPGFSAFFLWMYSMSTRLFLNTL 449 LS VA LR GF+ F Y+ + R+ NTL Sbjct: 611 LSSVAVALRDGFAPFAAETYARTFRILRNTL 641 >SPCC757.07c |ctt1|cta1|catalase|Schizosaccharomyces pombe|chr 3|||Manual Length = 512 Score = 25.8 bits (54), Expect = 5.0 Identities = 23/94 (24%), Positives = 41/94 (43%), Gaps = 2/94 (2%) Frame = -3 Query: 318 WLYLFSYQSHSDDPFYVLLCFSDTSAGVYCYRFLDDETILDHLTDVLSGVGVCDLIDFIW 139 W YL +++ V++ FSD Y YRF+D H ++ G +F + Sbjct: 168 WDYL---SQNAESIHQVMILFSDLGGTPYSYRFMDG--FSSHTYKFVNDKG-----EFYY 217 Query: 138 IQPHLLFTTSHNRGC--KPLLQLEGTHPDTVRSN 43 + H + T +G + L+G++PD R + Sbjct: 218 CKWHFI-TNQGTKGLTNEEAAALDGSNPDHARQD 250 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.8 bits (54), Expect = 5.0 Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 5/40 (12%) Frame = +1 Query: 235 HSRARVR-----KTQKHVERVVTVALVREEVQPMRVCHRR 339 H++AR+ K K E+++ AL RE V+P R H++ Sbjct: 645 HAKARLSSVVEVKDAKAAEKILRYALFREVVKPKRKKHKK 684 >SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 197 Score = 25.8 bits (54), Expect = 5.0 Identities = 12/38 (31%), Positives = 18/38 (47%) Frame = -3 Query: 513 LASQPSSCGCTP*AHACS*THYPSPSCRVSGIDACQSS 400 L S+ S+ C+P C +Y P+C V G S+ Sbjct: 17 LVSKTSASQCSPRYGTCGGIYYDGPTCCVVGSSCIYSN 54 >SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1077 Score = 25.4 bits (53), Expect = 6.6 Identities = 14/50 (28%), Positives = 23/50 (46%) Frame = +1 Query: 208 LVIKKPVAVHSRARVRKTQKHVERVVTVALVREEVQPMRVCHRRNYGYKD 357 L ++ PV +HS +TQ + + T +V VCH++ Y D Sbjct: 852 LKVEFPVDIHSEFASYETQFGITKRPTHYNTSWDVAKFEVCHQKFADYSD 901 >SPBC25H2.08c |mrs2||magnesium ion transporter Mrs2|Schizosaccharomyces pombe|chr 2|||Manual Length = 422 Score = 25.0 bits (52), Expect = 8.7 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = -2 Query: 544 GLSLVAKHLRPGFSAFFLWMYSMSTRLFLNTLPFAF 437 GL L A L GF A +Y M+ + L P+AF Sbjct: 335 GLKLSAMTLGLGFGAVVASLYGMNLQNGLENHPYAF 370 >SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 2812 Score = 25.0 bits (52), Expect = 8.7 Identities = 12/32 (37%), Positives = 16/32 (50%) Frame = -2 Query: 535 LVAKHLRPGFSAFFLWMYSMSTRLFLNTLPFA 440 LV +H RP F FFL Y+ + F +A Sbjct: 2582 LVCRHYRPVFRHFFLESYADPVQWFTTQTNYA 2613 >SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 25.0 bits (52), Expect = 8.7 Identities = 11/34 (32%), Positives = 17/34 (50%) Frame = -3 Query: 387 TSGAIFLKPFVFVPIVPSVAYAHWLYLFSYQSHS 286 +SG + LKP + P + W+ F +SHS Sbjct: 193 SSGGLLLKPAGRIGSSPIPGHGFWIESFDNKSHS 226 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,695,204 Number of Sequences: 5004 Number of extensions: 57577 Number of successful extensions: 188 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 175 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 269634532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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