BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20173 (728 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosacchar... 71 2e-13 SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c... 27 2.7 SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 27 2.7 SPAP7G5.06 |||amino acid permease, unknown 4|Schizosaccharomyces... 26 6.3 SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.4 >SPAC57A7.10c |sec21||coatomer gamma subunit Sec21 |Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 70.9 bits (166), Expect = 2e-13 Identities = 32/77 (41%), Positives = 49/77 (63%) Frame = +1 Query: 25 KDEDDNSSNPYQNLDKTIVLQETREFNQTLVIPRKCSLILTKILYLLNQGENFTTQEATD 204 K +DD + + N+++ V Q+ R FN + + PRK +L+KI YL+ GE+F ++AT+ Sbjct: 5 KKDDDGDESIFANVNQVTVTQDARAFNSSSISPRKSRRLLSKIAYLIYTGEHFQEKQATE 64 Query: 205 AFFATTKLFQSKEIMLR 255 FF TKLFQ K+ LR Sbjct: 65 LFFGITKLFQHKDPSLR 81 Score = 67.7 bits (158), Expect = 2e-12 Identities = 34/66 (51%), Positives = 48/66 (72%), Gaps = 1/66 (1%) Frame = +3 Query: 255 RMVYLCIKELSKLAQDVIIVTSSLTKDM-TGKEDLYRAAAIRALCSITDASMLQAIERYM 431 + VY+ IKELS +A+DVI++TSS+ KD TG+E +YR AIR+L + DA+ + AIER + Sbjct: 82 QFVYIIIKELSVVAEDVIMITSSIMKDTATGRETIYRPNAIRSLIRVIDANTVPAIERIL 141 Query: 432 KQAIVD 449 IVD Sbjct: 142 TTGIVD 147 Score = 31.9 bits (69), Expect = 0.096 Identities = 26/94 (27%), Positives = 42/94 (44%), Gaps = 24/94 (25%) Frame = +2 Query: 512 DLVRRWANEAQETINSDNA----------------------MVSYHALGVLVLSRRNDKL 625 D+V RW NE Q+ + S N + YHALG+L RR+D + Sbjct: 169 DIVSRWNNEVQDAVTSHNVGRKVASSPFFTSTLGYTPNASGISQYHALGLLYRIRRHDSI 228 Query: 626 STVKLVTRL--SKSHIKSPYSLCLLIRLAAQLCD 721 + KL+ L + + + ++ +LIR + L D Sbjct: 229 AMNKLLQLLVSNLGTVSNSHAFVMLIRYISSLMD 262 >SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 599 Score = 27.1 bits (57), Expect = 2.7 Identities = 10/26 (38%), Positives = 18/26 (69%) Frame = -3 Query: 90 FLKYYSFIEILVWVTAVIIFILNISL 13 FLK + FI ++ +T +++ +L ISL Sbjct: 284 FLKTFDFIGLVCVITGIVLILLGISL 309 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 27.1 bits (57), Expect = 2.7 Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 1/47 (2%) Frame = -3 Query: 642 TSLTVDNLSFRRDNTRTPNA*YDT-IALSELIVS*ASLAHRRTRSGR 505 TSLT+D + + D + +D + + LIV+ S A R RSGR Sbjct: 790 TSLTIDGIYYVVDPGFVKQSCFDPKLGMDSLIVTPISQAQARQRSGR 836 >SPAP7G5.06 |||amino acid permease, unknown 4|Schizosaccharomyces pombe|chr 1|||Manual Length = 583 Score = 25.8 bits (54), Expect = 6.3 Identities = 13/47 (27%), Positives = 24/47 (51%) Frame = -3 Query: 153 YFRKY**TFSRDNERLIKLSCFLKYYSFIEILVWVTAVIIFILNISL 13 YF Y TF + L + +Y++ I W+T ++F++ I+L Sbjct: 169 YFMNYFVTFPLE---LTTCAITFRYWTDINSCAWITIFLVFVICINL 212 >SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 25.4 bits (53), Expect = 8.4 Identities = 8/31 (25%), Positives = 19/31 (61%) Frame = -3 Query: 105 IKLSCFLKYYSFIEILVWVTAVIIFILNISL 13 + FL+ + F+ I+ T V++F+L +++ Sbjct: 273 VSFCVFLRDFDFVGIVTITTGVVLFLLGLNI 303 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,758,721 Number of Sequences: 5004 Number of extensions: 52370 Number of successful extensions: 179 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 178 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 343230174 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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