BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20173 (728 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / ga... 95 4e-20 At2g33330.1 68415.m04085 33 kDa secretory protein-related contai... 31 0.59 At5g11490.1 68418.m01341 adaptin family protein similar to SP|Q9... 31 0.78 At1g04520.1 68414.m00443 33 kDa secretory protein-related contai... 29 2.4 At5g61980.1 68418.m07779 ARF GTPase-activating domain-containing... 29 3.2 At4g18440.1 68417.m02736 adenylosuccinate lyase, putative / aden... 29 3.2 At3g22990.1 68416.m02899 expressed protein 29 3.2 At4g28820.1 68417.m04120 zinc finger (HIT type) family protein c... 28 5.5 At1g14970.1 68414.m01788 expressed protein contains Pfam PF03138... 28 5.5 >At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / gamma-2 coat protein, putative / gamma-2 COP, putative similar to SP|Q9UBF2 Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP) {Homo sapiens}; contains Pfam profile: PF01602 Adaptin N terminal region Length = 886 Score = 95.1 bits (226), Expect = 4e-20 Identities = 47/68 (69%), Positives = 53/68 (77%) Frame = +3 Query: 255 RMVYLCIKELSKLAQDVIIVTSSLTKDMTGKEDLYRAAAIRALCSITDASMLQAIERYMK 434 RMVYL IKELS + +VIIVTSSL KDM K D+YRA AIR LC I D ++L IERY+K Sbjct: 88 RMVYLIIKELSPSSDEVIIVTSSLMKDMNSKIDMYRANAIRVLCRIIDGTLLTQIERYLK 147 Query: 435 QAIVDKNP 458 QAIVDKNP Sbjct: 148 QAIVDKNP 155 Score = 94.3 bits (224), Expect = 7e-20 Identities = 43/82 (52%), Positives = 59/82 (71%) Frame = +1 Query: 10 LKRDVKDEDDNSSNPYQNLDKTIVLQETREFNQTLVIPRKCSLILTKILYLLNQGENFTT 189 +K+D +D+ +P+ ++K VLQE R FN V PR+CS ++TK+LYLLNQGE+FT Sbjct: 6 VKKDDDHDDELEYSPFMGIEKGAVLQEARVFNDPQVDPRRCSQVITKLLYLLNQGESFTK 65 Query: 190 QEATDAFFATTKLFQSKEIMLR 255 EAT+ FF+ TKLFQSK+ LR Sbjct: 66 VEATEVFFSVTKLFQSKDTGLR 87 Score = 73.3 bits (172), Expect = 1e-13 Identities = 32/69 (46%), Positives = 50/69 (72%) Frame = +2 Query: 509 PDLVRRWANEAQETINSDNAMVSYHALGVLVLSRRNDKLSTVKLVTRLSKSHIKSPYSLC 688 P++V+RW+NE QE I S +A+V +HAL +L R+ND+L+ KLV L++ ++SP + C Sbjct: 173 PEIVKRWSNEVQEGIQSRSALVQFHALALLHQIRQNDRLAVSKLVGSLTRGSVRSPLAQC 232 Query: 689 LLIRLAAQL 715 LLIR +Q+ Sbjct: 233 LLIRYTSQV 241 >At2g33330.1 68415.m04085 33 kDa secretory protein-related contains Pfam PF01657: Domain of unknown function, duplicated in 33 KDa secretory proteins Length = 304 Score = 31.5 bits (68), Expect = 0.59 Identities = 15/37 (40%), Positives = 19/37 (51%) Frame = +2 Query: 362 GGRNKSVVQHHGRLYATSYRTIYETGDCGQEPGRQFC 472 G VVQ HG YAT+Y ++Y G C + G C Sbjct: 170 GVMQNGVVQGHG-FYATTYESVYVLGQCEGDIGDSDC 205 >At5g11490.1 68418.m01341 adaptin family protein similar to SP|Q9WV76 Adapter-related protein complex 4 beta 1 subunit (Beta subunit of AP- 4) {Mus musculus}, beta-adaptin Drosophila 1 {Drosophila melanogaster} GI:434902; contains Pfam profile: PF01602 Adaptin N terminal region Length = 841 Score = 31.1 bits (67), Expect = 0.78 Identities = 16/68 (23%), Positives = 37/68 (54%), Gaps = 1/68 (1%) Frame = +3 Query: 255 RMVYLCIKELSKLAQDVIIVTSS-LTKDMTGKEDLYRAAAIRALCSITDASMLQAIERYM 431 +M YL + +K D+ ++T + L +D ++ + R A+R+LCS+ ++++ + + Sbjct: 81 KMCYLYVGNYAKGNPDLSLLTINFLQRDCKDEDPMIRGLALRSLCSLRVPNLVEYLVGPL 140 Query: 432 KQAIVDKN 455 + D N Sbjct: 141 GSGLKDNN 148 >At1g04520.1 68414.m00443 33 kDa secretory protein-related contains Pfam PF01657: Domain of unknown function, duplicated in 33 KDa secretory proteins Length = 307 Score = 29.5 bits (63), Expect = 2.4 Identities = 14/37 (37%), Positives = 18/37 (48%) Frame = +2 Query: 362 GGRNKSVVQHHGRLYATSYRTIYETGDCGQEPGRQFC 472 G VV HG YAT+Y ++Y G C + G C Sbjct: 168 GVMQNGVVSGHG-FYATTYESVYVLGQCEGDVGDTDC 203 >At5g61980.1 68418.m07779 ARF GTPase-activating domain-containing protein similar to GCN4-complementing protein (GCP1) GI:6465806 from [Arabidopsis thaliana] Length = 850 Score = 29.1 bits (62), Expect = 3.2 Identities = 18/41 (43%), Positives = 24/41 (58%) Frame = -2 Query: 430 IYRSIACSIEASVMLHNALIAAARYRSSLPVMSLVKEEVTM 308 +YR I CS EA V NAL A Y SLP+ +++ E T+ Sbjct: 686 VYRHIVCS-EADV---NALRGQASYTVSLPLSKMMQMEETL 722 >At4g18440.1 68417.m02736 adenylosuccinate lyase, putative / adenylosuccinase, putative similar to SP|P25739 Adenylosuccinate lyase (EC 4.3.2.2) (Adenylosuccinase) {Escherichia coli}; contains Pfam profile PF00206: Lyase Length = 536 Score = 29.1 bits (62), Expect = 3.2 Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 5/48 (10%) Frame = -2 Query: 586 CVIRHHRIVRIDCFLSFISPSP-----NKIRAVEDK*SAETNAAELTA 458 C+ HHR+ R+ C +S + SP K+ A++ S + + LTA Sbjct: 34 CLASHHRLPRVSCSVSTTTDSPKLVTSTKVTAMDGVSSRDLEMSNLTA 81 >At3g22990.1 68416.m02899 expressed protein Length = 460 Score = 29.1 bits (62), Expect = 3.2 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +3 Query: 207 FLCDNKTVSVQRNHAARMVYLCIKELSKLAQDVIIVTSSL 326 F+ DN+ V Q H V+ CI + + +D +VT+SL Sbjct: 224 FMPDNEVVMAQHRHCLETVFQCIHD--HMTEDEELVTNSL 261 >At4g28820.1 68417.m04120 zinc finger (HIT type) family protein contains Pfam profile: PF04438 HIT zinc finger Length = 173 Score = 28.3 bits (60), Expect = 5.5 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = +1 Query: 37 DNSSNPYQNLDKTIVLQETREFNQTLV 117 D+SSNP Q LD+ + ++ REF ++ Sbjct: 137 DSSSNPLQELDEAMGIEAFREFTDKIL 163 >At1g14970.1 68414.m01788 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'auxin-independent growth promoter -related' based on similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 562 Score = 28.3 bits (60), Expect = 5.5 Identities = 15/59 (25%), Positives = 32/59 (54%) Frame = -2 Query: 442 IACFIYRSIACSIEASVMLHNALIAAARYRSSLPVMSLVKEEVTMITSCANLLSSLMQR 266 I+C ++ A S +AS ++H + YRS V + ++ E+ + A+ +S++ +R Sbjct: 77 ISCMLFHLHAASFDASPIIHRRPAPGSVYRSP-QVYARLRGEIEADNTTADAISTIWKR 134 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,355,284 Number of Sequences: 28952 Number of extensions: 273570 Number of successful extensions: 747 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 719 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 747 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1594686376 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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