SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20165
         (748 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1al...   320   7e-90
X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alp...   315   2e-88
AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor 1-a...   202   3e-54
EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor 1-a...   172   3e-45
AF069739-1|AAC63272.2|  690|Apis mellifera translation initiatio...    33   0.003
AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9 methylt...    24   1.3  
DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450 monoo...    23   3.0  
AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase pro...    23   3.0  
D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.    23   4.0  
DQ325103-1|ABD14117.1|  182|Apis mellifera complementary sex det...    22   5.3  
AY569781-1|AAS75781.1|  461|Apis mellifera neuronal nicotinic ac...    22   5.3  
AB204559-1|BAD89804.1|  832|Apis mellifera soluble guanylyl cycl...    22   7.0  
DQ342041-1|ABC69933.1|  828|Apis mellifera STIP protein.               21   9.3  
AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase pro...    21   9.3  

>AF015267-1|AAC38959.1|  461|Apis mellifera elongation factor-1alpha
           F2 protein.
          Length = 461

 Score =  320 bits (787), Expect = 7e-90
 Identities = 166/236 (70%), Positives = 180/236 (76%), Gaps = 3/236 (1%)
 Frame = +2

Query: 50  MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229
           MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60

Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409
           DKLKAERE GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61  DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120

Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRR 589
           GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK              + +++     ++
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180

Query: 590 LATTQLLSLSCPFLDGTETTCWSFNQNALVQGMQWSVKKAS*R---KCLIEALDAI 748
           +          P + G          + +     W+V++   +   KCLIEALDAI
Sbjct: 181 IGYNPAAVAFVP-ISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAI 235



 Score =  116 bits (279), Expect = 2e-28
 Identities = 56/70 (80%), Positives = 60/70 (85%), Gaps = 1/70 (1%)
 Frame = +1

Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687
           +MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+  K    + 
Sbjct: 154 KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 213

Query: 688 -AVERKEGKL 714
             VERKEGK+
Sbjct: 214 WTVERKEGKV 223


>X52884-1|CAA37066.1|  461|Apis mellifera elongation factor 1 alpha
           protein.
          Length = 461

 Score =  315 bits (774), Expect = 2e-88
 Identities = 150/154 (97%), Positives = 151/154 (98%)
 Frame = +2

Query: 50  MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229
           MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1   MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60

Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409
           DKLKAERE GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 
Sbjct: 61  DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120

Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511
           GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154



 Score =  106 bits (254), Expect = 3e-25
 Identities = 51/68 (75%), Positives = 56/68 (82%), Gaps = 1/68 (1%)
 Frame = +1

Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687
           +MD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLE  PK    + 
Sbjct: 154 KMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKG 213

Query: 688 -AVERKEG 708
             VERK+G
Sbjct: 214 WKVERKDG 221


>AY208278-1|AAO48970.1|  274|Apis mellifera elongation factor
           1-alpha protein.
          Length = 274

 Score =  202 bits (493), Expect = 3e-54
 Identities = 110/179 (61%), Positives = 124/179 (69%), Gaps = 3/179 (1%)
 Frame = +2

Query: 221 WVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 400
           WVLDKLKAERE GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1   WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60

Query: 401 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHT 580
           AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK              + +++    
Sbjct: 61  AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120

Query: 581 SRRLATTQLLSLSCPFLDGTETTCWSFNQNALVQGMQWSVKKAS*R---KCLIEALDAI 748
            +++          P + G          + +     W+V++   +   KCLIEALDAI
Sbjct: 121 IKKIGYNPAAVAFVP-ISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAI 178



 Score =  116 bits (279), Expect = 2e-28
 Identities = 56/70 (80%), Positives = 60/70 (85%), Gaps = 1/70 (1%)
 Frame = +1

Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687
           +MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+  K    + 
Sbjct: 97  KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 156

Query: 688 -AVERKEGKL 714
             VERKEGK+
Sbjct: 157 WTVERKEGKV 166


>EF013389-1|ABK54743.1|  172|Apis mellifera elongation factor
           1-alpha protein.
          Length = 172

 Score =  172 bits (418), Expect = 3e-45
 Identities = 95/163 (58%), Positives = 109/163 (66%), Gaps = 3/163 (1%)
 Frame = +2

Query: 269 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 448
           DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1   DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60

Query: 449 REHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLATTQLLSLSCPF 628
           REHALLAFTLGVKQLIVGVNK              + +++     +++          P 
Sbjct: 61  REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVP- 119

Query: 629 LDGTETTCWSFNQNALVQGMQWSVKKAS*R---KCLIEALDAI 748
           + G          + +     W+V++   +   KCLIEALDAI
Sbjct: 120 ISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAI 162



 Score =  116 bits (279), Expect = 2e-28
 Identities = 56/70 (80%), Positives = 60/70 (85%), Gaps = 1/70 (1%)
 Frame = +1

Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687
           +MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+  K    + 
Sbjct: 81  KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 140

Query: 688 -AVERKEGKL 714
             VERKEGK+
Sbjct: 141 WTVERKEGKV 150


>AF069739-1|AAC63272.2|  690|Apis mellifera translation initiation
           factor 2 protein.
          Length = 690

 Score = 33.1 bits (72), Expect = 0.003
 Identities = 16/35 (45%), Positives = 18/35 (51%)
 Frame = +2

Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412
           VT +D PGH  FI     G    D  VL+VAA  G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229



 Score = 25.4 bits (53), Expect = 0.57
 Identities = 9/18 (50%), Positives = 13/18 (72%)
 Frame = +2

Query: 62  KTHINIVVIGHVDSGKST 115
           K H  + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160


>AM050259-1|CAJ18340.1|  683|Apis mellifera putative H3K9
           methyltransferase protein.
          Length = 683

 Score = 24.2 bits (50), Expect = 1.3
 Identities = 11/15 (73%), Positives = 11/15 (73%)
 Frame = +2

Query: 71  INIVVIGHVDSGKST 115
           INI  IGHV  GKST
Sbjct: 43  INIGTIGHVAHGKST 57


>DQ244074-1|ABB36784.1|  517|Apis mellifera cytochrome P450
           monooxygenase protein.
          Length = 517

 Score = 23.0 bits (47), Expect = 3.0
 Identities = 10/17 (58%), Positives = 12/17 (70%)
 Frame = -2

Query: 63  FSLPIFG*SRITNCV*Y 13
           FSLPIFG   I +C+ Y
Sbjct: 57  FSLPIFGTRWIFSCIGY 73


>AB253417-1|BAE86928.1|  567|Apis mellifera alpha-glucosidase
           protein.
          Length = 567

 Score = 23.0 bits (47), Expect = 3.0
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +2

Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394
           T KYY    D P +  FIKN+   ++ +D   LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327


>D79208-1|BAA11466.1|  567|Apis mellifera alpha-glucosidase protein.
          Length = 567

 Score = 22.6 bits (46), Expect = 4.0
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +2

Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394
           T KYY    D P +  FIKN+   ++ +D   L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327


>DQ325103-1|ABD14117.1|  182|Apis mellifera complementary sex
           determiner protein.
          Length = 182

 Score = 22.2 bits (45), Expect = 5.3
 Identities = 10/44 (22%), Positives = 19/44 (43%)
 Frame = +1

Query: 496 RRSKQMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 627
           R  K + S    Y+   +       ++Y KK+ YN   +  +P+
Sbjct: 77  REPKIISSLSNNYNYSNYNNYNNNYNNYNKKLYYNINYIEQIPV 120


>AY569781-1|AAS75781.1|  461|Apis mellifera neuronal nicotinic
           acetylcholine Apisa7-2 subunit protein.
          Length = 461

 Score = 22.2 bits (45), Expect = 5.3
 Identities = 9/29 (31%), Positives = 14/29 (48%)
 Frame = -2

Query: 684 P*TKAFWLKLQHVVSVPSRNGHESDSSWV 598
           P    F L L H++ V  +N   + + WV
Sbjct: 30  PLAVVFGLSLHHIIDVDEKNQILTTNCWV 58


>AB204559-1|BAD89804.1|  832|Apis mellifera soluble guanylyl cyclase
           beta-3 protein.
          Length = 832

 Score = 21.8 bits (44), Expect = 7.0
 Identities = 6/14 (42%), Positives = 11/14 (78%)
 Frame = +3

Query: 627 FWMARRQHVGASTK 668
           +W+ +R+H  +STK
Sbjct: 593 YWLEKREHRSSSTK 606


>DQ342041-1|ABC69933.1|  828|Apis mellifera STIP protein.
          Length = 828

 Score = 21.4 bits (43), Expect = 9.3
 Identities = 9/28 (32%), Positives = 16/28 (57%)
 Frame = +1

Query: 487 TAHRRSKQMDSTEPPYSEPRFEEIKKEV 570
           T+  R++   S  PP ++PR E + + V
Sbjct: 725 TSLERTQPTMSQMPPTAQPRMERLAEAV 752


>AB253416-1|BAE86927.1|  580|Apis mellifera alpha-glucosidase
           protein.
          Length = 580

 Score = 21.4 bits (43), Expect = 9.3
 Identities = 7/20 (35%), Positives = 13/20 (65%)
 Frame = +2

Query: 512 WIPLNHHTVSPDLRKSRRKY 571
           W+P+N +  S +L   +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 213,608
Number of Sequences: 438
Number of extensions: 4482
Number of successful extensions: 32
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 29
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23388480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -