BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20165 (748 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 320 7e-90 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 315 2e-88 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 202 3e-54 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 172 3e-45 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.3 DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.0 AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.0 D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.0 DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex det... 22 5.3 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 22 5.3 AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cycl... 22 7.0 DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. 21 9.3 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 9.3 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 320 bits (787), Expect = 7e-90 Identities = 166/236 (70%), Positives = 180/236 (76%), Gaps = 3/236 (1%) Frame = +2 Query: 50 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409 DKLKAERE GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120 Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRR 589 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK + +++ ++ Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180 Query: 590 LATTQLLSLSCPFLDGTETTCWSFNQNALVQGMQWSVKKAS*R---KCLIEALDAI 748 + P + G + + W+V++ + KCLIEALDAI Sbjct: 181 IGYNPAAVAFVP-ISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAI 235 Score = 116 bits (279), Expect = 2e-28 Identities = 56/70 (80%), Positives = 60/70 (85%), Gaps = 1/70 (1%) Frame = +1 Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687 +MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+ K + Sbjct: 154 KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 213 Query: 688 -AVERKEGKL 714 VERKEGK+ Sbjct: 214 WTVERKEGKV 223 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 315 bits (774), Expect = 2e-88 Identities = 150/154 (97%), Positives = 151/154 (98%) Frame = +2 Query: 50 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 229 MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60 Query: 230 DKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 409 DKLKAERE GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120 Query: 410 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 511 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154 Score = 106 bits (254), Expect = 3e-25 Identities = 51/68 (75%), Positives = 56/68 (82%), Gaps = 1/68 (1%) Frame = +1 Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687 +MD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHGDNMLE PK + Sbjct: 154 KMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKG 213 Query: 688 -AVERKEG 708 VERK+G Sbjct: 214 WKVERKDG 221 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 202 bits (493), Expect = 3e-54 Identities = 110/179 (61%), Positives = 124/179 (69%), Gaps = 3/179 (1%) Frame = +2 Query: 221 WVLDKLKAERELGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 400 WVLDKLKAERE GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60 Query: 401 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHT 580 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK + +++ Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120 Query: 581 SRRLATTQLLSLSCPFLDGTETTCWSFNQNALVQGMQWSVKKAS*R---KCLIEALDAI 748 +++ P + G + + W+V++ + KCLIEALDAI Sbjct: 121 IKKIGYNPAAVAFVP-ISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAI 178 Score = 116 bits (279), Expect = 2e-28 Identities = 56/70 (80%), Positives = 60/70 (85%), Gaps = 1/70 (1%) Frame = +1 Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687 +MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+ K + Sbjct: 97 KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 156 Query: 688 -AVERKEGKL 714 VERKEGK+ Sbjct: 157 WTVERKEGKV 166 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 172 bits (418), Expect = 3e-45 Identities = 95/163 (58%), Positives = 109/163 (66%), Gaps = 3/163 (1%) Frame = +2 Query: 269 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 448 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60 Query: 449 REHALLAFTLGVKQLIVGVNKWIPLNHHTVSPDLRKSRRKYPHTSRRLATTQLLSLSCPF 628 REHALLAFTLGVKQLIVGVNK + +++ +++ P Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVP- 119 Query: 629 LDGTETTCWSFNQNALVQGMQWSVKKAS*R---KCLIEALDAI 748 + G + + W+V++ + KCLIEALDAI Sbjct: 120 ISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAI 162 Score = 116 bits (279), Expect = 2e-28 Identities = 56/70 (80%), Positives = 60/70 (85%), Gaps = 1/70 (1%) Frame = +1 Query: 508 QMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLELQPKCLGSRD 687 +MDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE+ K + Sbjct: 81 KMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKG 140 Query: 688 -AVERKEGKL 714 VERKEGK+ Sbjct: 141 WTVERKEGKV 150 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 33.1 bits (72), Expect = 0.003 Identities = 16/35 (45%), Positives = 18/35 (51%) Frame = +2 Query: 308 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 412 VT +D PGH FI G D VL+VAA G Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229 Score = 25.4 bits (53), Expect = 0.57 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +2 Query: 62 KTHINIVVIGHVDSGKST 115 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.3 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +2 Query: 71 INIVVIGHVDSGKST 115 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monooxygenase protein. Length = 517 Score = 23.0 bits (47), Expect = 3.0 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = -2 Query: 63 FSLPIFG*SRITNCV*Y 13 FSLPIFG I +C+ Y Sbjct: 57 FSLPIFGTRWIFSCIGY 73 >AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 23.0 bits (47), Expect = 3.0 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = +2 Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394 T KYY D P + FIKN+ ++ +D LI Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327 >D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. Length = 567 Score = 22.6 bits (46), Expect = 4.0 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +2 Query: 293 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 394 T KYY D P + FIKN+ ++ +D L+ Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327 >DQ325103-1|ABD14117.1| 182|Apis mellifera complementary sex determiner protein. Length = 182 Score = 22.2 bits (45), Expect = 5.3 Identities = 10/44 (22%), Positives = 19/44 (43%) Frame = +1 Query: 496 RRSKQMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 627 R K + S Y+ + ++Y KK+ YN + +P+ Sbjct: 77 REPKIISSLSNNYNYSNYNNYNNNYNNYNKKLYYNINYIEQIPV 120 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 22.2 bits (45), Expect = 5.3 Identities = 9/29 (31%), Positives = 14/29 (48%) Frame = -2 Query: 684 P*TKAFWLKLQHVVSVPSRNGHESDSSWV 598 P F L L H++ V +N + + WV Sbjct: 30 PLAVVFGLSLHHIIDVDEKNQILTTNCWV 58 >AB204559-1|BAD89804.1| 832|Apis mellifera soluble guanylyl cyclase beta-3 protein. Length = 832 Score = 21.8 bits (44), Expect = 7.0 Identities = 6/14 (42%), Positives = 11/14 (78%) Frame = +3 Query: 627 FWMARRQHVGASTK 668 +W+ +R+H +STK Sbjct: 593 YWLEKREHRSSSTK 606 >DQ342041-1|ABC69933.1| 828|Apis mellifera STIP protein. Length = 828 Score = 21.4 bits (43), Expect = 9.3 Identities = 9/28 (32%), Positives = 16/28 (57%) Frame = +1 Query: 487 TAHRRSKQMDSTEPPYSEPRFEEIKKEV 570 T+ R++ S PP ++PR E + + V Sbjct: 725 TSLERTQPTMSQMPPTAQPRMERLAEAV 752 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 21.4 bits (43), Expect = 9.3 Identities = 7/20 (35%), Positives = 13/20 (65%) Frame = +2 Query: 512 WIPLNHHTVSPDLRKSRRKY 571 W+P+N + S +L +R+Y Sbjct: 443 WLPVNENYKSLNLAAQKREY 462 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 213,608 Number of Sequences: 438 Number of extensions: 4482 Number of successful extensions: 32 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 29 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 23388480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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