BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20159 (589 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 176 2e-45 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 98 1e-21 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 96 3e-21 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 93 4e-20 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 62 8e-11 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 59 5e-10 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 49 4e-07 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 39 6e-04 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 30 0.29 SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe... 28 0.88 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 27 1.5 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.7 SPAC144.16 |||DUF59 family protein|Schizosaccharomyces pombe|chr... 26 3.5 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 3.5 SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 26 4.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 176 bits (429), Expect = 2e-45 Identities = 79/90 (87%), Positives = 82/90 (91%) Frame = +2 Query: 239 GTEGLYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLIE 418 G + YVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LL E Sbjct: 48 GQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTE 107 Query: 419 APLNPKANREKMTQIMFETFNTPAMYVAIQ 508 APLNPK+NREKMTQI+FETFN PA YVAIQ Sbjct: 108 APLNPKSNREKMTQIIFETFNAPAFYVAIQ 137 Score = 106 bits (254), Expect = 3e-24 Identities = 46/51 (90%), Positives = 50/51 (98%) Frame = +3 Query: 102 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDS 254 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDS Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDS 52 Score = 34.3 bits (75), Expect = 0.013 Identities = 14/15 (93%), Positives = 15/15 (100%) Frame = +3 Query: 543 GIVLDSGDGVSHTVP 587 GIVLDSGDGV+HTVP Sbjct: 150 GIVLDSGDGVTHTVP 164 Score = 25.0 bits (52), Expect = 8.2 Identities = 11/11 (100%), Positives = 11/11 (100%) Frame = +1 Query: 511 VLSLYASGRTT 543 VLSLYASGRTT Sbjct: 139 VLSLYASGRTT 149 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 97.9 bits (233), Expect = 1e-21 Identities = 40/95 (42%), Positives = 66/95 (69%) Frame = +2 Query: 224 RDGRYGTEGLYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 403 + G + + VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 404 VLLIEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 508 +LL E P+NP ANREKM + MFE + +YVAIQ Sbjct: 103 ILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQ 137 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +3 Query: 108 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 212 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 Score = 30.3 bits (65), Expect = 0.22 Identities = 11/15 (73%), Positives = 14/15 (93%) Frame = +3 Query: 543 GIVLDSGDGVSHTVP 587 G+V+DSGDGV+H VP Sbjct: 150 GVVVDSGDGVTHIVP 164 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 96.3 bits (229), Expect = 3e-21 Identities = 41/88 (46%), Positives = 61/88 (69%), Gaps = 1/88 (1%) Frame = +2 Query: 251 LYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLIEAPL 427 ++VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LL E PL Sbjct: 56 MFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115 Query: 428 NPKANREKMTQIMFETFNTPAMYVAIQP 511 N+EK+ + +ET N PA+ ++QP Sbjct: 116 ANIRNKEKIAEYFYETLNVPALSFSLQP 143 Score = 58.8 bits (136), Expect = 5e-10 Identities = 25/44 (56%), Positives = 32/44 (72%) Frame = +3 Query: 120 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKD 251 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKD 55 Score = 27.9 bits (59), Expect = 1.2 Identities = 10/15 (66%), Positives = 14/15 (93%) Frame = +3 Query: 543 GIVLDSGDGVSHTVP 587 GIVL+ GDG++H+VP Sbjct: 155 GIVLECGDGLTHSVP 169 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 92.7 bits (220), Expect = 4e-20 Identities = 39/86 (45%), Positives = 54/86 (62%), Gaps = 1/86 (1%) Frame = +2 Query: 254 YVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLIEAPLN 430 ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LL E PLN Sbjct: 75 FIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLN 134 Query: 431 PKANREKMTQIMFETFNTPAMYVAIQ 508 P NRE +IMFE+FN +Y+A+Q Sbjct: 135 PPENRENTAEIMFESFNCAGLYIAVQ 160 Score = 40.7 bits (91), Expect = 2e-04 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +3 Query: 120 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 203 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 Score = 30.7 bits (66), Expect = 0.16 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = +3 Query: 531 RSYHGIVLDSGDGVSHTVP 587 RS G V+DSGDGV+H +P Sbjct: 177 RSLTGTVVDSGDGVTHIIP 195 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 61.7 bits (143), Expect = 8e-11 Identities = 36/96 (37%), Positives = 51/96 (53%), Gaps = 2/96 (2%) Frame = +2 Query: 227 DGR--YGTEGLYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 400 DGR +G E +Y +S G + +K I +G V NWD +W + +L+ P EH Sbjct: 46 DGRRLFGEEYIY-----KSNPG-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEH 99 Query: 401 PVLLIEAPLNPKANREKMTQIMFETFNTPAMYVAIQ 508 P+L+ E NP NR K + MFE+ PA Y+A Q Sbjct: 100 PILITEPFDNPPENRVKTLETMFESLRCPATYLAKQ 135 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +3 Query: 105 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 206 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 58.8 bits (136), Expect = 5e-10 Identities = 29/95 (30%), Positives = 52/95 (54%), Gaps = 1/95 (1%) Frame = +2 Query: 224 RDGRYGTEGLYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 400 RD + VG++ + G ++ + P E +++NWD ME++ +TF +L + EH Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116 Query: 401 PVLLIEAPLNPKANREKMTQIMFETFNTPAMYVAI 505 P+ + E NP R MT+++FE +N P++ I Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSVAYGI 151 Score = 35.9 bits (79), Expect = 0.004 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 120 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 215 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 49.2 bits (112), Expect = 4e-07 Identities = 25/87 (28%), Positives = 45/87 (51%), Gaps = 2/87 (2%) Frame = +2 Query: 254 YVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLIEAPL 427 YV DE Q I + +K +GI+ +W+ W +L+V P E+ +++ E Sbjct: 52 YVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSW 111 Query: 428 NPKANREKMTQIMFETFNTPAMYVAIQ 508 NP++ R+++ + FE + PA Y+ Q Sbjct: 112 NPQSVRQQIMEAAFEQLHVPAFYLTKQ 138 Score = 36.7 bits (81), Expect = 0.003 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +3 Query: 105 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 206 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 38.7 bits (86), Expect = 6e-04 Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 4/73 (5%) Frame = +2 Query: 305 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLIEAPLNPKANREKMTQIMFE 472 PI+ G V +W+ ++ W H Y+ L P + +PV L+ +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 473 TFNTPAMYVAIQP 511 P +A +P Sbjct: 172 ECQVPGFTIAYEP 184 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 29.9 bits (64), Expect = 0.29 Identities = 19/66 (28%), Positives = 29/66 (43%) Frame = -1 Query: 337 IPVSDDSVFDGVFEGQDTSFALCLISYIESFCPIPTITP*WRGLPTIEGNTARGASSPAK 158 +P + +V +TSFAL S S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 157 PALHIP 140 P L+ P Sbjct: 123 PQLNSP 128 >SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 28.3 bits (60), Expect = 0.88 Identities = 13/32 (40%), Positives = 17/32 (53%) Frame = -2 Query: 573 RHRRRSPARYRGTTGRVQREHGWMATYMAGVL 478 R +R +P + T R E+ W A Y AGVL Sbjct: 192 RSKRLTPLLIKEVTRRCHLENVWQAVYTAGVL 223 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 27.5 bits (58), Expect = 1.5 Identities = 13/32 (40%), Positives = 22/32 (68%), Gaps = 1/32 (3%) Frame = +2 Query: 389 PEEHPVLLIE-APLNPKANREKMTQIMFETFN 481 PEE ++E APL+ + +KM +++FET+N Sbjct: 174 PEEDFFRVLETAPLHAEEVSKKMKELLFETYN 205 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 2.7 Identities = 15/45 (33%), Positives = 18/45 (40%) Frame = -1 Query: 235 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 101 PT+ P P N S P PL+TTN TS+S Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC144.16 |||DUF59 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 179 Score = 26.2 bits (55), Expect = 3.5 Identities = 19/70 (27%), Positives = 32/70 (45%) Frame = +2 Query: 380 RVAPEEHPVLLIEAPLNPKANREKMTQIMFETFNTPAMYVAIQPCSRCTRPVVPRYRAGL 559 ++ EHP+ L + + + E + + +++ T + I CS CT + R L Sbjct: 65 KINDPEHPLTLAQLSVVKLEDIEVVDNVEGDSYITVHITPTIPHCSMCTL-IGLCIRVRL 123 Query: 560 RRRCLPHRAH 589 RCLP R H Sbjct: 124 -ERCLPPRFH 132 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 3.5 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -1 Query: 241 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 149 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 663 Score = 25.8 bits (54), Expect = 4.7 Identities = 19/60 (31%), Positives = 28/60 (46%) Frame = +2 Query: 368 YNELRVAPEEHPVLLIEAPLNPKANREKMTQIMFETFNTPAMYVAIQPCSRCTRPVVPRY 547 Y+EL +A +E P L P PK + + N A + +P S+ TRP +P Y Sbjct: 23 YSELPIAYQEIP--LQSLPPYPKVASKLKGVVAGGKENNIASFQ--KPSSKATRPYIPSY 78 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,585,576 Number of Sequences: 5004 Number of extensions: 54452 Number of successful extensions: 180 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 148 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 178 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 254167452 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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