BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20148 (679 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1223.02 |nmt1|thi3|no message in thiamine Nmt1|Schizosacchar... 31 0.20 SPAC19B12.09 |srp14||signal recognition particle subunit Srp14|S... 28 1.4 SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosac... 27 2.5 SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 26 4.4 SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 5.8 SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 25 7.6 SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar... 25 7.6 >SPCC1223.02 |nmt1|thi3|no message in thiamine Nmt1|Schizosaccharomyces pombe|chr 3|||Manual Length = 346 Score = 30.7 bits (66), Expect = 0.20 Identities = 26/98 (26%), Positives = 47/98 (47%), Gaps = 9/98 (9%) Frame = +1 Query: 109 CC*ILTMVLLSNDEFLA---ELTKLFQKARLAGSITM------TMKRYDGRSKPQPREEL 261 CC T++ +++DEF+A + K F +A + ++ M T K Y K + EL Sbjct: 194 CCCFCTILYIAHDEFIAKHPDKIKAFLRAIHSATLDMLKDPVQTYKEYI-HFKREMGSEL 252 Query: 262 LQLKIPNTNAYYGHNLRVKRYQQLWNSVMSKSSQLHIL 375 + + AY+ H+ + + WN V + S +L I+ Sbjct: 253 HREQFERCFAYFSHD--ISNVPRDWNKVTNYSKRLGII 288 >SPAC19B12.09 |srp14||signal recognition particle subunit Srp14|Schizosaccharomyces pombe|chr 1|||Manual Length = 106 Score = 27.9 bits (59), Expect = 1.4 Identities = 14/32 (43%), Positives = 23/32 (71%), Gaps = 2/32 (6%) Frame = +1 Query: 130 VLLSNDEFLAELTKLFQ--KARLAGSITMTMK 219 +LLSN+EFL +LT L Q +++ GS+ ++ K Sbjct: 1 MLLSNEEFLKKLTDLLQTHQSKGTGSVYLSQK 32 >SPBC8D2.19 |mde3||serine/threonine protein kinase Mde3|Schizosaccharomyces pombe|chr 2|||Manual Length = 559 Score = 27.1 bits (57), Expect = 2.5 Identities = 10/23 (43%), Positives = 17/23 (73%) Frame = +1 Query: 205 TMTMKRYDGRSKPQPREELLQLK 273 T+ +K+ SKP+P+ ELL+L+ Sbjct: 49 TVAVKKLKNSSKPKPKHELLKLR 71 >SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 745 Score = 26.2 bits (55), Expect = 4.4 Identities = 14/43 (32%), Positives = 25/43 (58%) Frame = +1 Query: 163 LTKLFQKARLAGSITMTMKRYDGRSKPQPREELLQLKIPNTNA 291 LTKL + R+ G I +T K G+S +E+ Q ++P +++ Sbjct: 46 LTKLDKDIRIQGWIRVTTKFLQGKSGSVKVQEIPQDQLPKSSS 88 >SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 25.8 bits (54), Expect = 5.8 Identities = 13/32 (40%), Positives = 18/32 (56%), Gaps = 1/32 (3%) Frame = +2 Query: 356 VLNCIF*SAE-NEC*WFETLEKAQEKSNGDSV 448 +L C F + + N+C WF L A +NG SV Sbjct: 480 LLKCTFSNLDGNDCSWFSYLPNAIVLANGTSV 511 >SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 25.4 bits (53), Expect = 7.6 Identities = 13/34 (38%), Positives = 17/34 (50%) Frame = -1 Query: 175 KVSLTLLKTHRCSIKPLLKFNNNNWNLYVSSAVD 74 K SLT KT + +KP K N+ + SS D Sbjct: 84 KASLTETKTEKAKVKPKAKKKNSKEKISKSSKQD 117 >SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 653 Score = 25.4 bits (53), Expect = 7.6 Identities = 18/43 (41%), Positives = 22/43 (51%), Gaps = 6/43 (13%) Frame = +1 Query: 217 KRYDGRS-KPQPREELL-----QLKIPNTNAYYGHNLRVKRYQ 327 K Y GR+ K REE L Q+K+ N Y G NL +K Q Sbjct: 330 KLYVGRAQKKHEREEELRKRYEQMKLEKMNKYQGVNLFIKNLQ 372 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,602,914 Number of Sequences: 5004 Number of extensions: 48763 Number of successful extensions: 145 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 145 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 311890690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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