Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20132
         (695 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    91   2e-19
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    70   4e-13
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    57   3e-09
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    56   5e-09
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    46   5e-06
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    43   4e-05
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    39   8e-04
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ...    36   0.007
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    29   0.48 
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma...    28   1.1  
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb...    28   1.5  
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p...    27   3.4  
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces...    26   4.5  
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ...    26   4.5  
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce...    26   5.9  
SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49 |...    26   5.9  

>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 90.6 bits (215), Expect = 2e-19
 Identities = 41/78 (52%), Positives = 53/78 (67%), Gaps = 1/78 (1%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG 433
           +APWCGHCK+LAPEY  AA +L  E+  I L +VD T+E DL   Y +RGYPTL  F+NG
Sbjct: 47  YAPWCGHCKALAPEYESAADEL--EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNG 104

Query: 434 SPI-DYSGGRQADDIISW 484
             I  YSG R+ D ++ +
Sbjct: 105 KQISQYSGPRKHDALVKY 122



 Score = 68.9 bits (161), Expect = 6e-13
 Identities = 33/77 (42%), Positives = 51/77 (66%), Gaps = 3/77 (3%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG 433
           +APWCGHCK+LAP Y K A + + ++S + +AK+DAT E D+  S  + G+PT+ FF+  
Sbjct: 381 YAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVAKIDAT-ENDI--SVSISGFPTIMFFKAN 436

Query: 434 ---SPIDYSGGRQADDI 475
              +P+ Y G R  +D+
Sbjct: 437 DKVNPVRYEGDRTLEDL 453


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 69.7 bits (163), Expect = 4e-13
 Identities = 32/76 (42%), Positives = 47/76 (61%), Gaps = 2/76 (2%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RN 430
           +A WCGHCKSLAP Y +    L E+ + + + K+DA    D+A+ Y + G+PTL +F  +
Sbjct: 47  YATWCGHCKSLAPVYEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPD 105

Query: 431 GS-PIDYSGGRQADDI 475
           GS P+ YS  R  D +
Sbjct: 106 GSEPVQYSNARDVDSL 121



 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 26/80 (32%), Positives = 43/80 (53%), Gaps = 3/80 (3%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF--- 424
           +A WCG+CK LAP Y +   K+ + E  +++ K++A    D+   + V  +PT+KFF   
Sbjct: 166 YADWCGYCKRLAPTY-ETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKD 224

Query: 425 RNGSPIDYSGGRQADDIISW 484
               P  Y G R  + +I +
Sbjct: 225 DKDKPELYEGDRSLESLIEY 244


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 56.8 bits (131), Expect = 3e-09
 Identities = 25/68 (36%), Positives = 40/68 (58%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG 433
           +A WCG CK+++P +++ A+K A  +     AKV+  +++ +A   GV+  PT  FF NG
Sbjct: 27  YADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQRQIASGLGVKAMPTFVFFENG 84

Query: 434 SPIDYSGG 457
             ID   G
Sbjct: 85  KQIDMLTG 92


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 56.0 bits (129), Expect = 5e-09
 Identities = 29/75 (38%), Positives = 40/75 (53%), Gaps = 6/75 (8%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF--- 424
           +APWCG+CK L P Y K A+ L     P+     DA Q + +   Y V+G+PT+K     
Sbjct: 56  YAPWCGYCKKLVPTYQKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPS 114

Query: 425 RNGSPI---DYSGGR 460
             GS +   DY+G R
Sbjct: 115 SKGSSLSSTDYNGDR 129


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 23/64 (35%), Positives = 32/64 (50%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG 433
           +A WCG CK L P       KL+E+        V+A +  D+A+  GV   PT+  FR G
Sbjct: 43  YADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKG 98

Query: 434 SPID 445
             +D
Sbjct: 99  QELD 102


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 20/63 (31%), Positives = 32/63 (50%)
 Frame = +2

Query: 257 APWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGS 436
           A WCG CK++AP++     + +   S     KVD  Q  ++A   GV   P+   ++NG 
Sbjct: 27  ATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQLSEIAAEAGVHAMPSFFLYKNGE 82

Query: 437 PID 445
            I+
Sbjct: 83  KIE 85


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 23/73 (31%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
 Frame = +2

Query: 233 GVHFS*IHAPWCGHCKSLAPEYAKAATKLAE--EESPIKLAKVDATQEQDLAESYGVRGY 406
           G  F   + P CG CK L P +     K  E  E S     +VD ++E  L+    +R  
Sbjct: 43  GTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKE--LSSCANIRAV 100

Query: 407 PTLKFFRNGSPID 445
           PTL  ++NG  ++
Sbjct: 101 PTLYLYQNGEIVE 113



 Score = 30.7 bits (66), Expect = 0.21
 Identities = 13/73 (17%), Positives = 34/73 (46%)
 Frame = +2

Query: 266 CGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPID 445
           C  C  ++  +   A ++      + +A ++    +   + Y ++ +PT  FF+  + ++
Sbjct: 310 CDDCDDVSTAWYAMANRM---RGKLNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVE 366

Query: 446 YSGGRQADDIISW 484
           Y G     D++S+
Sbjct: 367 YVGLPNEGDLVSF 379


>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 632

 Score = 35.5 bits (78), Expect = 0.007
 Identities = 14/71 (19%), Positives = 37/71 (52%)
 Frame = +2

Query: 272 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYS 451
           HC+      A  ++     +  +K+A+V+  +E+++   + ++ +PT + F+    I Y+
Sbjct: 208 HCEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYN 267

Query: 452 GGRQADDIISW 484
           G  +   ++S+
Sbjct: 268 GPLKYQQLLSY 278



 Score = 26.6 bits (56), Expect = 3.4
 Identities = 11/24 (45%), Positives = 14/24 (58%)
 Frame = +2

Query: 377 LAESYGVRGYPTLKFFRNGSPIDY 448
           LA  YG +  P++   RNG PI Y
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVY 366


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 29.5 bits (63), Expect = 0.48
 Identities = 14/52 (26%), Positives = 25/52 (48%)
 Frame = +2

Query: 254 HAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYP 409
           +APW   CK +   +     + A++       K++A +  D+AES+ V   P
Sbjct: 28  YAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVP 75


>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 318

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 20/65 (30%), Positives = 29/65 (44%)
 Frame = +2

Query: 272 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYS 451
           +C  L P   +AA  L + +SP  L  VDA   + L  S+GVR    +     G+    +
Sbjct: 183 YCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVRKAVIITLGSQGAYYKSA 240

Query: 452 GGRQA 466
            G  A
Sbjct: 241 NGESA 245


>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1778

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 15/44 (34%), Positives = 21/44 (47%)
 Frame = -1

Query: 617 RKVLALVELWSEKKPNTITVLASISSLACSAEVTSTEGGQSSSS 486
           +K  +L + W   + +    LASISS A SA + ST       S
Sbjct: 673 KKAASLPQFWLSPRSHNTARLASISSFAKSAVMNSTSASGKPKS 716


>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 387

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
 Frame = +1

Query: 481 LAEEEDWPPSVE-VTSAEQAKELIDANTVIVFGFFSDQSSTRAKTFLST 624
           L+EEE+   SVE + S  + KE   A + +    F D+SST  K  + T
Sbjct: 233 LSEEEESLKSVESIISNLENKESTTATSTLTDAGFGDKSSTAGKHNVET 281


>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 3699

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -3

Query: 363  VASTFASLIGDSSSASFVAALAYSGARDLQW 271
            V++ F SL+G  S   FVA    +  R L+W
Sbjct: 1039 VSNIFKSLVGSMSCVEFVAEARETINRSLEW 1069


>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 422

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 14/56 (25%), Positives = 25/56 (44%)
 Frame = +1

Query: 505 PSVEVTSAEQAKELIDANTVIVFGFFSDQSSTRAKTFLSTAQVVDDQVFAIVSDEK 672
           PS+  TSA+        N +       D+++T+A        + DDQ+  +  D+K
Sbjct: 350 PSLTTTSAQATTNA--TNNITTTTAAQDENATKAALIAQLMALTDDQINVLPPDQK 403


>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 747

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 9/12 (75%), Positives = 11/12 (91%)
 Frame = -1

Query: 119 NTHCDIIGTFLL 84
           NTHCDI+ +FLL
Sbjct: 275 NTHCDIVTSFLL 286


>SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 175

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 9/16 (56%), Positives = 12/16 (75%)
 Frame = +1

Query: 484 AEEEDWPPSVEVTSAE 531
           +E  DWPPSV++ S E
Sbjct: 143 SEYADWPPSVQIDSEE 158


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,677,375
Number of Sequences: 5004
Number of extensions: 49851
Number of successful extensions: 167
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)