BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20130 (414 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 111 3e-26 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 67 1e-12 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 66 2e-12 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 59 3e-10 SPAPB24D3.06c |||DUF1749 family protein|Schizosaccharomyces pomb... 27 1.5 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 2.0 SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster ... 25 6.1 SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei... 24 8.1 SPBP4H10.21c |sld5||GINS complex subunit Sld5|Schizosaccharomyce... 24 8.1 SPBC336.02 |||18S rRNA dimethylase|Schizosaccharomyces pombe|chr... 24 8.1 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 111 bits (268), Expect = 3e-26 Identities = 53/84 (63%), Positives = 59/84 (70%) Frame = +3 Query: 3 NNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 182 N WAKGHYTEGAEL D+VLDVVR+EAE+CD LQGFQ KIREE Sbjct: 99 NIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREE 158 Query: 183 YPDRIMNTYSVVPSPKVSDTVVEP 254 YPDR+M T+SV P+PK SDTVVEP Sbjct: 159 YPDRMMATFSVAPAPKSSDTVVEP 182 Score = 71.3 bits (167), Expect = 5e-14 Identities = 31/44 (70%), Positives = 37/44 (84%) Frame = +2 Query: 257 NATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNH 388 NATLS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y DLNH Sbjct: 184 NATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNH 227 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 66.9 bits (156), Expect = 1e-12 Identities = 32/84 (38%), Positives = 48/84 (57%) Frame = +3 Query: 3 NNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 182 NN+A+GHYT G E++DSVL+ +R+ A++C LQGF ++ E Sbjct: 105 NNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNME 164 Query: 183 YPDRIMNTYSVVPSPKVSDTVVEP 254 Y + +SV P+P+VS +VVEP Sbjct: 165 YGKKSNLQFSVYPAPQVSTSVVEP 188 Score = 52.4 bits (120), Expect = 3e-08 Identities = 21/43 (48%), Positives = 30/43 (69%) Frame = +2 Query: 257 NATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLN 385 N+ L+ H ++N+D T+ +DNEA YDIC R L + PTY +LN Sbjct: 190 NSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLN 232 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 66.5 bits (155), Expect = 2e-12 Identities = 33/84 (39%), Positives = 46/84 (54%) Frame = +3 Query: 3 NNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 182 NN+A+GHYT G ELVD V D +R+ A++C LQGF ++ E Sbjct: 101 NNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAME 160 Query: 183 YPDRIMNTYSVVPSPKVSDTVVEP 254 Y + +SV P+P+VS +VVEP Sbjct: 161 YTKKSKLQFSVYPAPQVSTSVVEP 184 Score = 45.6 bits (103), Expect = 3e-06 Identities = 18/43 (41%), Positives = 28/43 (65%) Frame = +2 Query: 257 NATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLN 385 N+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y +LN Sbjct: 186 NSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLN 228 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 58.8 bits (136), Expect = 3e-10 Identities = 28/85 (32%), Positives = 44/85 (51%), Gaps = 1/85 (1%) Frame = +3 Query: 3 NNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 182 NNWA G Y+ + + ++D++ +EA+ D L+GF ++ + Sbjct: 102 NNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDR 160 Query: 183 YPDRIMNTYSVVP-SPKVSDTVVEP 254 YP +I+ TYSV P S VSD VV+P Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQP 185 Score = 27.9 bits (59), Expect = 0.66 Identities = 14/43 (32%), Positives = 20/43 (46%) Frame = +2 Query: 257 NATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLN 385 N+ L++ +L N D +DN AL I L PT+ N Sbjct: 187 NSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQN 229 >SPAPB24D3.06c |||DUF1749 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 316 Score = 26.6 bits (56), Expect = 1.5 Identities = 14/40 (35%), Positives = 19/40 (47%) Frame = +3 Query: 237 DTVVEPTTPHFQFIN*LKTQTKPTVSTTRLSTISASAR*N 356 D V P+T Q +N + +PT S + LS I A N Sbjct: 250 DEFVSPSTDKAQLLNRFRESIRPTTSNSSLSGIIPGATHN 289 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 26.2 bits (55), Expect = 2.0 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +3 Query: 12 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 101 A+GH G ELV + D +RK++E+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPAC1F7.11c |||transcription factor zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 782 Score = 24.6 bits (51), Expect = 6.1 Identities = 9/26 (34%), Positives = 13/26 (50%) Frame = +2 Query: 320 EALYDICFRTLKLSTPTYGDLNHWCR 397 +A Y +CF + Y DL + CR Sbjct: 567 QAAYALCFPNTDYALTAYNDLQYICR 592 >SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1462 Score = 24.2 bits (50), Expect = 8.1 Identities = 10/21 (47%), Positives = 14/21 (66%) Frame = +2 Query: 251 TDNATLSVHQLVENTDETYCI 313 T N L+V+ +V N DET+ I Sbjct: 1418 TFNVCLAVNTIVNNVDETFSI 1438 >SPBP4H10.21c |sld5||GINS complex subunit Sld5|Schizosaccharomyces pombe|chr 2|||Manual Length = 214 Score = 24.2 bits (50), Expect = 8.1 Identities = 10/26 (38%), Positives = 13/26 (50%) Frame = +1 Query: 292 HRRNLLYRQRGSLRYLLPHAKTIHTH 369 H LLY +YLL H + +H H Sbjct: 107 HPNLLLYLSSPERQYLLRHQQIVHRH 132 >SPBC336.02 |||18S rRNA dimethylase|Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 24.2 bits (50), Expect = 8.1 Identities = 9/24 (37%), Positives = 15/24 (62%) Frame = +2 Query: 263 TLSVHQLVENTDETYCIDNEALYD 334 T S+ ++VEN T+C NE + + Sbjct: 238 TSSIIEMVENNYRTWCSQNERMVE 261 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,653,448 Number of Sequences: 5004 Number of extensions: 29901 Number of successful extensions: 105 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 97 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 102 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 144287194 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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