BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20126
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 76 5e-15
SPAP8A3.10 |||mitochondrial intermembrane space protein sorting ... 42 6e-05
SPAC21E11.07 ||SPAC2C4.01|glycine cleavage T-protein|Schizosacch... 30 0.34
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 27 2.4
SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosacch... 27 2.4
SPBC28F2.10c |kap1||chromatin remodeling complex subunit Ngg1 |S... 25 7.2
>SPBC36.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 75.8 bits (178), Expect = 5e-15
Identities = 41/87 (47%), Positives = 55/87 (63%), Gaps = 1/87 (1%)
Frame = +2
Query: 251 KKVVD-GVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFC 427
+KV+D GVL+T RL++ PRW LI A+ CY E S V+ R +TL T+NLTF
Sbjct: 41 RKVLDNGVLYTERLITCHQALPRWILKLIDGAQDCYIRETSYVDLKARTLTLLTSNLTFS 100
Query: 428 HYIAVDETVRYTPHPSDSSKTLLKQEA 508
+ VDETV Y+PHP + T+ +QEA
Sbjct: 101 DRLRVDETVTYSPHP-ELEATVFQQEA 126
Score = 46.0 bits (104), Expect = 5e-06
Identities = 20/43 (46%), Positives = 28/43 (65%)
Frame = +3
Query: 132 MKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKL 260
MKI+ S H F +P+E V+ A W+KYPN VI D ++RK+
Sbjct: 1 MKIFESCHLFQYPFEQVSAAHWQKYPNEHATHVIAVDTLDRKV 43
>SPAP8A3.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 171
Score = 42.3 bits (95), Expect = 6e-05
Identities = 24/78 (30%), Positives = 41/78 (52%)
Frame = +2
Query: 263 DGVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAV 442
+G L+T RL+ + PRWA L+ K Y E S ++P ++++ +T NL + V
Sbjct: 47 EGRLYTERLLVKQGRLPRWASDLLNVNK-SYILERSVIDPSKQELKSETFNLDHVKILRV 105
Query: 443 DETVRYTPHPSDSSKTLL 496
E R+ + SKT++
Sbjct: 106 IEYSRFIQSSENCSKTIV 123
Score = 35.9 bits (79), Expect = 0.005
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +3
Query: 138 IWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVER 254
I T + N W TV+ A +YPNP + V+ DV+ER
Sbjct: 4 ICTDKTELNASWNTVSSAWLTRYPNPYSLHVVSADVLER 42
>SPAC21E11.07 ||SPAC2C4.01|glycine cleavage
T-protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 325
Score = 29.9 bits (64), Expect = 0.34
Identities = 14/41 (34%), Positives = 26/41 (63%), Gaps = 4/41 (9%)
Frame = +1
Query: 469 SFRFLKN----ITKTRSLVTVQGVPLSSYMEDLLTNKISLN 579
SF+ +N + ++SL+ V+GV +++ L TNKI+L+
Sbjct: 14 SFQLFRNYSVCLNSSKSLIRVEGVDAVKFLQGLTTNKITLD 54
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 27.1 bits (57), Expect = 2.4
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -2
Query: 449 FHQQQCN-DRMLGLLFLKSSVAVLGLLLISH*HN 351
FH Q N D++L + FL V ++G+L +H HN
Sbjct: 443 FHPPQMNTDQLLLVSFLGLVVNLVGILAFNHGHN 476
>SPAC26F1.09 |gyp51||GTPase activating protein Gyp51
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1031
Score = 27.1 bits (57), Expect = 2.4
Identities = 29/107 (27%), Positives = 51/107 (47%), Gaps = 4/107 (3%)
Frame = -2
Query: 482 RNLKDVEYTVQFHQQQCNDRMLGLL--FLKSSVAVLGLLLISH*HNIFLQYQLAPGPIVE 309
+ L+ +EY +Q ++++ LL F+++ + VL + I + +V
Sbjct: 562 QKLEILEY-LQSQSSTVSNQVFTLLSNFIQNPLFVLDECFDEFRNLILMHNSHTVHTVVW 620
Query: 308 KTIWN*PIYEYEVHHLQLSFHNICPYDGWIHR--IRIFSPCCLSNCF 174
KTI + Y+YE+ + LS N C D I + R F+P LS+ F
Sbjct: 621 KTISSWTSYDYEMQYSSLSIKN-CDSDKAIRKDLDRTFAPEILSHFF 666
>SPBC28F2.10c |kap1||chromatin remodeling complex subunit Ngg1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 7.2
Identities = 12/21 (57%), Positives = 14/21 (66%), Gaps = 2/21 (9%)
Frame = +1
Query: 364 DIRS--KPNTATDDFKNNKPN 420
D+RS ATDDF +NKPN
Sbjct: 209 DVRSLVAGTPATDDFSHNKPN 229
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,844,930
Number of Sequences: 5004
Number of extensions: 61659
Number of successful extensions: 143
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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