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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20126
         (654 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g13070.1 68418.m01498 MSF1-like family protein similar to px1...    48   5e-06
At4g27000.1 68417.m03884 RNA-binding protein 45 (RBP45), putativ...    29   2.7  
At4g22790.1 68417.m03289 MATE efflux family protein contains Pfa...    29   3.6  
At3g26930.1 68416.m03369 hypothetical protein                          28   4.7  
At2g36580.1 68415.m04486 pyruvate kinase, putative similar to py...    28   4.7  

>At5g13070.1 68418.m01498 MSF1-like family protein similar to px19
           [Gallus gallus] GI:969170; contains Pfam profile
           PF04707: MSF1-like conserved region
          Length = 183

 Score = 48.0 bits (109), Expect = 5e-06
 Identities = 29/81 (35%), Positives = 44/81 (54%), Gaps = 1/81 (1%)
 Frame = +2

Query: 266 GVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAVD 445
           G LHT R ++     P +   +IG   IC+  E + V+   R M L T N++   +I V+
Sbjct: 53  GKLHTTRALTIHAPGPWFLHRIIGQ-DICHCVESTVVDGKSRSMQLTTKNISLKKFIEVE 111

Query: 446 ETVRYTPHPSD-SSKTLLKQE 505
           E +RY PHP + S+ T+  QE
Sbjct: 112 ERIRYDPHPDNPSAWTVCSQE 132



 Score = 45.6 bits (103), Expect = 3e-05
 Identities = 18/47 (38%), Positives = 28/47 (59%), Gaps = 4/47 (8%)
 Frame = +3

Query: 132 MKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIG----TDVVERKL 260
           +K +  EH + HPWE V+ A+WRK+ +P N  ++      D + RKL
Sbjct: 2   VKAYRQEHVYKHPWERVSAASWRKFADPENKRILSHILEVDTLNRKL 48


>At4g27000.1 68417.m03884 RNA-binding protein 45 (RBP45), putative
           DNA binding protein ACBF - Nicotiana tabacum,
           PID:g1899188
          Length = 415

 Score = 29.1 bits (62), Expect = 2.7
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = +1

Query: 478 FLKNITKTRSLVTVQGVPLSSYMEDLLTNKISLNAGKGRQA 600
           F+ + T  R+L T  G P+ S  +    N   L AG+ RQA
Sbjct: 128 FVNHATAERNLQTYNGAPMPSSEQAFRLNWAQLGAGERRQA 168


>At4g22790.1 68417.m03289 MATE efflux family protein contains Pfam
           profile PF01554: Uncharacterized membrane protein family
          Length = 491

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 8/19 (42%), Positives = 12/19 (63%)
 Frame = -2

Query: 224 WIHRIRIFSPCCLSNCFPW 168
           W+  I++  PCCL+ C  W
Sbjct: 253 WLTLIKLSGPCCLTVCLEW 271


>At3g26930.1 68416.m03369 hypothetical protein
          Length = 409

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 13/32 (40%), Positives = 21/32 (65%)
 Frame = +1

Query: 481 LKNITKTRSLVTVQGVPLSSYMEDLLTNKISL 576
           L  ++ T   VTV G P++SY+E+LL +  S+
Sbjct: 210 LVEVSLTDRQVTVDGYPIASYVENLLRSLTSV 241


>At2g36580.1 68415.m04486 pyruvate kinase, putative similar to
           pyruvate kinase, cytosolic isozyme [Glycine max]
           SWISS-PROT:Q42806
          Length = 527

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 15/46 (32%), Positives = 20/46 (43%)
 Frame = -3

Query: 253 LSTTSVPMTAGFIGLGYFRHAA*ATVSHGWLNVCSDVQIFMFFSNI 116
           +ST  V     F+ L Y RHA     +   LN C D+     F+ I
Sbjct: 212 ISTWGVQNKIDFLSLSYCRHAEDVRQARELLNSCGDLSQTQIFAKI 257


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,578,612
Number of Sequences: 28952
Number of extensions: 312748
Number of successful extensions: 707
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 694
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 707
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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