BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20123 (581 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 138 4e-34 SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 137 1e-33 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 137 1e-33 SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 137 1e-33 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 137 1e-33 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 86 4e-18 SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 43 3e-05 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 41 1e-04 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 40 2e-04 SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi... 30 0.28 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 2.6 SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 27 2.6 SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 26 4.6 SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 25 6.1 SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schiz... 25 8.1 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 25 8.1 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 138 bits (335), Expect = 4e-34 Identities = 68/75 (90%), Positives = 71/75 (94%) Frame = +2 Query: 8 RRRVKMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRT 187 R R MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRT Sbjct: 72 RLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRT 131 Query: 188 LSDYNIQKESTLHLV 232 LSDYNIQKESTLHLV Sbjct: 132 LSDYNIQKESTLHLV 146 Score = 138 bits (335), Expect = 4e-34 Identities = 68/75 (90%), Positives = 71/75 (94%) Frame = +2 Query: 8 RRRVKMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRT 187 R R MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRT Sbjct: 148 RLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRT 207 Query: 188 LSDYNIQKESTLHLV 232 LSDYNIQKESTLHLV Sbjct: 208 LSDYNIQKESTLHLV 222 Score = 138 bits (335), Expect = 4e-34 Identities = 68/75 (90%), Positives = 71/75 (94%) Frame = +2 Query: 8 RRRVKMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRT 187 R R MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRT Sbjct: 224 RLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRT 283 Query: 188 LSDYNIQKESTLHLV 232 LSDYNIQKESTLHLV Sbjct: 284 LSDYNIQKESTLHLV 298 Score = 138 bits (335), Expect = 4e-34 Identities = 68/75 (90%), Positives = 71/75 (94%) Frame = +2 Query: 8 RRRVKMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRT 187 R R MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRT Sbjct: 300 RLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRT 359 Query: 188 LSDYNIQKESTLHLV 232 LSDYNIQKESTLHLV Sbjct: 360 LSDYNIQKESTLHLV 374 Score = 137 bits (332), Expect = 1e-33 Identities = 66/70 (94%), Positives = 69/70 (98%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 203 IQKESTLHLV 232 IQKESTLHLV Sbjct: 61 IQKESTLHLV 70 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 137 bits (332), Expect = 1e-33 Identities = 66/70 (94%), Positives = 69/70 (98%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 203 IQKESTLHLV 232 IQKESTLHLV Sbjct: 61 IQKESTLHLV 70 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 137 bits (332), Expect = 1e-33 Identities = 66/70 (94%), Positives = 69/70 (98%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 203 IQKESTLHLV 232 IQKESTLHLV Sbjct: 61 IQKESTLHLV 70 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 137 bits (332), Expect = 1e-33 Identities = 66/70 (94%), Positives = 69/70 (98%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 203 IQKESTLHLV 232 IQKESTLHLV Sbjct: 61 IQKESTLHLV 70 Score = 79.4 bits (187), Expect = 4e-16 Identities = 36/70 (51%), Positives = 46/70 (65%) Frame = +1 Query: 256 KRKKKNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVMEDR 435 KRKKK Y+TP LAVL++YKV+++G + RLRREC CGA FMA +DR Sbjct: 79 KRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHKDR 136 Query: 436 HYCGKCHSTM 465 YCG+CH T+ Sbjct: 137 LYCGRCHLTL 146 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 137 bits (332), Expect = 1e-33 Identities = 66/70 (94%), Positives = 69/70 (98%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 MQIFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYN Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60 Query: 203 IQKESTLHLV 232 IQKESTLHLV Sbjct: 61 IQKESTLHLV 70 Score = 79.4 bits (187), Expect = 4e-16 Identities = 36/70 (51%), Positives = 46/70 (65%) Frame = +1 Query: 256 KRKKKNYSTPXXXXXXXXXXXLAVLRFYKVDENGKIHRLRRECTGEQCGAGVFMAVMEDR 435 KRKKK Y+TP LAVL++YKV+++G + RLRREC CGA FMA +DR Sbjct: 79 KRKKKTYTTPKKIKHKHKKVELAVLKYYKVEDDGSVKRLRREC--PNCGASTFMANHKDR 136 Query: 436 HYCGKCHSTM 465 YCG+CH T+ Sbjct: 137 LYCGRCHLTL 146 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 85.8 bits (203), Expect = 4e-18 Identities = 36/70 (51%), Positives = 53/70 (75%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 M I VKTLTGK I L+++P+D + +K ++++KEGIPP+QQRLI+AGKQ+ D + Y+ Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60 Query: 203 IQKESTLHLV 232 ++ S LHLV Sbjct: 61 LEGGSVLHLV 70 >SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 43.2 bits (97), Expect = 3e-05 Identities = 20/58 (34%), Positives = 33/58 (56%) Frame = +2 Query: 50 GKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTL 223 GK LE+EP++T +K ++ +PP +Q++I G QL+D L I+ +TL Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 41.1 bits (92), Expect = 1e-04 Identities = 20/65 (30%), Positives = 38/65 (58%), Gaps = 1/65 (1%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDT-IENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDY 199 M + K L + + +DT I +K KIQ ++ +Q+LI++G+ L D +T+ +Y Sbjct: 1 MNLTFKNLQQQKFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEY 60 Query: 200 NIQKE 214 NI+++ Sbjct: 61 NIKEQ 65 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 40.3 bits (90), Expect = 2e-04 Identities = 20/70 (28%), Positives = 36/70 (51%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 + + +K + + V+ ++ +K I I +QRLI+AG+ L+D +L Y Sbjct: 4 ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63 Query: 203 IQKESTLHLV 232 IQ ++HLV Sbjct: 64 IQDGHSIHLV 73 >SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schizosaccharomyces pombe|chr 2|||Manual Length = 73 Score = 29.9 bits (64), Expect = 0.28 Identities = 16/69 (23%), Positives = 31/69 (44%) Frame = +2 Query: 23 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYN 202 +++ GK + ++ P DT+ + K + + G P + L +D TL+DY Sbjct: 2 IEVLCNDRLGKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKWHSVFKDNITLADYE 61 Query: 203 IQKESTLHL 229 I +L + Sbjct: 62 IHDGMSLEM 70 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 26.6 bits (56), Expect = 2.6 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = -1 Query: 212 PSGYCSPRGYVHLPAAFQQRSTSVDWEGF 126 PS + PRG V+ P+ ++R + + GF Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45 >SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 26.6 bits (56), Expect = 2.6 Identities = 14/54 (25%), Positives = 24/54 (44%), Gaps = 1/54 (1%) Frame = +1 Query: 325 VLRFY-KVDENGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 483 V+R Y ++ +N + R C E F V++D H KC + ++ D Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277 >SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr 3|||Manual Length = 577 Score = 25.8 bits (54), Expect = 4.6 Identities = 14/43 (32%), Positives = 26/43 (60%), Gaps = 2/43 (4%) Frame = +2 Query: 5 TRRRVKMQIFVKTLTGKTITLEVEPSDTIE--NVKAKIQDKEG 127 T++ + + F LT T T+E EP+ TI+ ++A++ +K G Sbjct: 13 TQQYLSKRKFEDALTFITKTIEQEPNPTIDLFELRAQVYEKSG 55 >SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochromatin assembly Hrr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1015 Score = 25.4 bits (53), Expect = 6.1 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -3 Query: 456 VTLPAVVTVLHHRHEHSGAALLAC 385 VTL A+ T+L + H H L+AC Sbjct: 402 VTLKAIETLLENTHSHVLPILVAC 425 >SPAC227.15 |||protein phosphatase regulatory subunit Reg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 873 Score = 25.0 bits (52), Expect = 8.1 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -3 Query: 528 SSRERSFRLEGTYLFVVVFEDHRAVTLPAV 439 +S + S+ L TYL F++HR + AV Sbjct: 149 NSHQNSYSLNETYLSYDFFDNHRGASSSAV 178 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 25.0 bits (52), Expect = 8.1 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +2 Query: 134 PNQQRLIFAGKQLEDGRTLSDYNI 205 PNQ AGK+L+ R L DY+I Sbjct: 476 PNQGDTRKAGKRLKYSRNLDDYHI 499 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,234,372 Number of Sequences: 5004 Number of extensions: 43420 Number of successful extensions: 109 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 96 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 106 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 250133048 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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