Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20122
         (642 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ...    27   2.3  
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz...    27   3.0  
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||...    26   4.0  
SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M...    26   4.0  
SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subun...    25   7.0  
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom...    25   7.0  
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ...    25   7.0  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    25   9.3  
SPBP8B7.13 |||conserved fungal protein|Schizosaccharomyces pombe...    25   9.3  
SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces p...    25   9.3  
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa...    25   9.3  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    25   9.3  

>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1364

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 11/31 (35%), Positives = 19/31 (61%)
 Frame = -2

Query: 356 NKNRTLLNPVRNSMGCVSNSLLDLTGTVSGS 264
           N + TLL+P+R S   +   ++DLT  +S +
Sbjct: 429 NSHLTLLSPIRPSSRSMEEQIMDLTQPISST 459


>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 676

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 16/49 (32%), Positives = 19/49 (38%), Gaps = 1/49 (2%)
 Frame = +2

Query: 191 TNIGFVDCGEE*TMHSGCRPRLSFWIHSP-FPSNPTKSSKHNPLSFSPD 334
           T +  VDC E      G  PR   W+  P          K N  SF+PD
Sbjct: 463 TEMKLVDCEEGNYNSHGHPPRGEIWLRGPSLTRGYLNRDKENKESFTPD 511


>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1367

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 9/18 (50%), Positives = 14/18 (77%)
 Frame = +2

Query: 278 FPSNPTKSSKHNPLSFSP 331
           FPSN  +++K+ P+SF P
Sbjct: 128 FPSNKIRTTKYTPVSFIP 145


>SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1115

 Score = 26.2 bits (55), Expect = 4.0
 Identities = 15/38 (39%), Positives = 20/38 (52%)
 Frame = +2

Query: 254 LSFWIHSPFPSNPTKSSKHNPLSFSPDSAKSCSC*GRY 367
           LS  +  PFP+ PT    H  LS    +A  CSC G++
Sbjct: 246 LSSLVPIPFPAGPTIIRMHPKLS---TTAVVCSCSGQF 280


>SPAC8C9.03 |cgs1||cAMP-dependent protein kinase regulatory subunit
           Cgs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 412

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 12/33 (36%), Positives = 16/33 (48%)
 Frame = -3

Query: 529 GGVLEFYSSRDHLTVGPLALSPTATYSPVLSPL 431
           G   +F S  + +  GP A+ P A Y P L  L
Sbjct: 54  GDESDFLSELNDMVAGPEAIGPDAKYVPELGGL 86


>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1375

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 13/23 (56%), Positives = 14/23 (60%)
 Frame = -1

Query: 243  QPLCIVHSSPQSTNPIFVYYYIV 175
            Q +  VH   Q T P FVYYYIV
Sbjct: 1288 QAISRVHRIGQ-TRPTFVYYYIV 1309


>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 962

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 17/43 (39%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
 Frame = -2

Query: 380 FSQIYI-YLNKNRTLLN--PVRNSMGCVSNSLLDLTGTVSGSK 261
           F QI + YLN+ RTLLN   + NS   +  + L LT  +   K
Sbjct: 865 FGQILVVYLNRERTLLNFYLIENSK-TIDEATLQLTDLIQAIK 906


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
           Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 10/34 (29%), Positives = 20/34 (58%)
 Frame = -2

Query: 443 PQSPFTTLSRRDGVDVL*SDFFSQIYIYLNKNRT 342
           P SP+  ++ R+G + L  D+  + ++ L K+ T
Sbjct: 110 PYSPYFYIAAREGKEALVDDYLKKKFVGLIKSTT 143


>SPBP8B7.13 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 251

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
 Frame = -3

Query: 493 LTVGPLALSPTATYSPVLS-PLSRHSQE 413
           L   PL ++PT + SP+L+  LS  SQE
Sbjct: 171 LATSPLQINPTTSKSPLLNKKLSSTSQE 198


>SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1028

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
 Frame = -1

Query: 333 SGEKLNGLCFEL--FVGFDGNGEWIQKES 253
           S + L+GL  +   F GF+ N EWI  ++
Sbjct: 257 SNDSLDGLSSQKNEFTGFNSNSEWIPNDT 285


>SPCC550.03c |||RNA helicase involved in mRNA
           catabolism|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1213

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 11/24 (45%), Positives = 17/24 (70%), Gaps = 1/24 (4%)
 Frame = +3

Query: 195 ISDSSIVVKN-EQCIAAVALDFPF 263
           + DSS  ++N +Q +  +ALDFPF
Sbjct: 256 VVDSSAPIENFQQLVPEMALDFPF 279


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.0 bits (52), Expect = 9.3
 Identities = 11/29 (37%), Positives = 17/29 (58%)
 Frame = -2

Query: 497 PLNSRAVSSFAHRYLFSGPQSPFTTLSRR 411
           P  S+  ++  HR+ +S PQ P +  SRR
Sbjct: 58  PFLSKRSNNSPHRFSYSPPQHPASINSRR 86


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,746,630
Number of Sequences: 5004
Number of extensions: 58488
Number of successful extensions: 163
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)