BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20116
(699 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 34 0.022
SPBC25H2.06c |hrf1||COPII-coated vesicle component Hrf1 |Schizos... 27 2.6
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po... 27 2.6
SPBC2A9.08c |sec22||SNARE Sec22|Schizosaccharomyces pombe|chr 2|... 25 7.9
SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces pombe... 25 7.9
>SPAPB8E5.07c |||ribosome biogenesis protein
Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1163
Score = 33.9 bits (74), Expect = 0.022
Identities = 26/96 (27%), Positives = 46/96 (47%), Gaps = 7/96 (7%)
Frame = -3
Query: 646 FRVLLLRIGRGSLEVRQFCSSPRCSVLAKYTVSRNSWSSEPRVTSII---VRNVVISLGF 476
F+ + S+E+R ++ CSV+ + NS++ + S I +R++ L +
Sbjct: 324 FKAFFTLLESESMEIRLQTATTICSVIGCLDTTPNSFAVVEEICSFICDALRDIRFRLAY 383
Query: 475 PQRHERGQSLRDFL----DPSRLKALEAVDQLQHHE 380
P+ + SL D L DP + ALE +D L+ E
Sbjct: 384 PECFQIISSLCDKLGPHSDPYLIPALEVIDYLRGSE 419
>SPBC25H2.06c |hrf1||COPII-coated vesicle component Hrf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 293
Score = 27.1 bits (57), Expect = 2.6
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -2
Query: 341 SWSKELRRSEDFGHSFVRCPP 279
SW+++LRRSE G + CPP
Sbjct: 111 SWARKLRRSEINGSAEGYCPP 131
>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1313
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = -3
Query: 532 SEPRVTSIIVRNVVISLGFPQRHERGQSLRDFLDPSRLKALEAVDQLQHHE 380
S P V + ++ LGFP++ E ++ ++ + L AL L HE
Sbjct: 675 SVPEVRASVLTAFTTFLGFPEKTEEVVAVETYIAIAALAALSDASPLVRHE 725
>SPBC2A9.08c |sec22||SNARE Sec22|Schizosaccharomyces pombe|chr
2|||Manual
Length = 209
Score = 25.4 bits (53), Expect = 7.9
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +3
Query: 42 KAGRRIFCPAVWNSNSPVNVTLMLY 116
KA RR+ A+W PV++ +L+
Sbjct: 175 KAARRVNLEALWRQYGPVSIIALLF 199
>SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 523
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -3
Query: 475 PQRHERGQSLRDFLDPSRLKALEAVDQLQH 386
P E Q +RD L PSRL L + ++QH
Sbjct: 214 PSIMESRQWIRDLLLPSRLGLLLDIGRIQH 243
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,895,366
Number of Sequences: 5004
Number of extensions: 58994
Number of successful extensions: 186
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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