BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20109 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2D10.11c |||nucleosome assembly protein Nap2 |Schizosaccharo... 43 3e-05 SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 42 9e-05 SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr 1||... 27 1.6 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 26 3.6 SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizos... 26 4.8 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 6.3 SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 6.3 SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 25 8.3 SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT |Schizosa... 25 8.3 >SPBC2D10.11c |||nucleosome assembly protein Nap2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 43.2 bits (97), Expect = 3e-05 Identities = 28/70 (40%), Positives = 38/70 (54%), Gaps = 2/70 (2%) Frame = +3 Query: 255 LKCLQDIK-VQMHEDPISFTLEFYFAPNEYFTNTVLTKEYLMKCKPDEESPLEFEGPEIY 431 L+ L DI+ + D + LEF F N+YFTN +LTK Y K D+ SP G +Y Sbjct: 192 LRSLSDIRFTNLSGDVHGYKLEFEFDSNDYFTNKILTKTYYYK---DDLSP---SGEFLY 245 Query: 432 S-CKGCEINW 458 +G +INW Sbjct: 246 DHAEGDKINW 255 Score = 35.1 bits (77), Expect = 0.008 Identities = 13/31 (41%), Positives = 19/31 (61%) Frame = +1 Query: 163 PNVKGIPDFWYNIFRNVSMLSEMMQEHDEPI 255 P+ KGIP+FW NV ++ EM+ DE + Sbjct: 161 PDPKGIPEFWLTCLHNVFLVGEMITPEDENV 191 Score = 28.3 bits (60), Expect = 0.89 Identities = 12/27 (44%), Positives = 15/27 (55%) Frame = +2 Query: 509 KSRGSVRTVTKSVQAXSFFNFFNPPTL 589 + R V +V SFFNFF+PP L Sbjct: 274 RKTNQTRLVRTTVPNDSFFNFFSPPQL 300 >SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 393 Score = 41.5 bits (93), Expect = 9e-05 Identities = 25/75 (33%), Positives = 34/75 (45%) Frame = +3 Query: 147 RASNGSQCKGYPRLLVQHIQECLNAQRNDART**TNLKCLQDIKVQMHEDPISFTLEFYF 326 + G KG P + ++ L+ L L DI++ E P F LEF F Sbjct: 154 KQEGGDDTKGIPEFWLTAMKNVLSLSEMITPEDEGALSHLVDIRISYMEKP-GFKLEFEF 212 Query: 327 APNEYFTNTVLTKEY 371 A N +FTN +LTK Y Sbjct: 213 AENPFFTNKILTKTY 227 Score = 39.9 bits (89), Expect = 3e-04 Identities = 21/63 (33%), Positives = 33/63 (52%) Frame = +1 Query: 61 DDTEEEELARAVQNAAITEGEEKKDDKAIEPPMDPNVKGIPDFWYNIFRNVSMLSEMMQE 240 D+ EEE+ + + A E +E ++ + + KGIP+FW +NV LSEM+ Sbjct: 126 DEPTEEEIKKG-EAADENEKKEPTSSESKKQEGGDDTKGIPEFWLTAMKNVLSLSEMITP 184 Query: 241 HDE 249 DE Sbjct: 185 EDE 187 Score = 35.1 bits (77), Expect = 0.008 Identities = 16/28 (57%), Positives = 17/28 (60%) Frame = +2 Query: 509 KSRGSVRTVTKSVQAXSFFNFFNPPTLP 592 K+ R V SV SFFNFFNPPT P Sbjct: 269 KNTKQTRVVKVSVPRDSFFNFFNPPTPP 296 >SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 27.5 bits (58), Expect = 1.6 Identities = 12/29 (41%), Positives = 17/29 (58%) Frame = +2 Query: 503 EAKSRGSVRTVTKSVQAXSFFNFFNPPTL 589 E S ++R++TK VQ SF+ F P L Sbjct: 39 EDASEEAIRSITKIVQDLSFYEFSGSPAL 67 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 26.2 bits (55), Expect = 3.6 Identities = 12/29 (41%), Positives = 18/29 (62%) Frame = +1 Query: 97 QNAAITEGEEKKDDKAIEPPMDPNVKGIP 183 +N+ + E EEK D +AIE + +V G P Sbjct: 554 KNSLVNEAEEKNDLEAIEAAKNFHVNGKP 582 >SPAC13G6.03 |gpi7||GPI anchor biosynthesis protein Gpi7 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 25.8 bits (54), Expect = 4.8 Identities = 16/55 (29%), Positives = 32/55 (58%), Gaps = 4/55 (7%) Frame = -3 Query: 171 YIGIH-WRLDSLVILLFLTLSDGSI---LYRPS*LFFFSVITPWVETFIIIRFIC 19 Y G+ + + ++ ILLF ++ G++ L++P + SV T W+ + I + F+C Sbjct: 659 YTGLDSYNIFAVGILLFTSVFAGALWWCLHQPKRMMDRSVKTFWIMSSISLTFLC 713 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +1 Query: 73 EEELARAVQNAAITEGEEKKDDKAIEPPMDPNVKGI 180 EEEL+ ++ N + E++KD+ +P P++ + Sbjct: 475 EEELSDSLSNDFGIDAEKEKDENLSKPEHHPSITSV 510 >SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1583 Score = 25.4 bits (53), Expect = 6.3 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = +3 Query: 144 YRASNGSQCKGYPRLLVQHIQEC 212 YR SNG + Y L V+ IQ C Sbjct: 489 YRLSNGKSIQYYSTLFVRLIQSC 511 >SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces pombe|chr 2|||Manual Length = 1309 Score = 25.0 bits (52), Expect = 8.3 Identities = 15/50 (30%), Positives = 22/50 (44%), Gaps = 1/50 (2%) Frame = +3 Query: 384 KPDEES-PLEFEGPEIYSCKGCEINWNAGXXXXXXXXXXXXXXSPVGQFA 530 +PDE PL F G + S +GCE+ ++G P+G A Sbjct: 1044 EPDENILPLHFYGELVKSPQGCEVLESSGHFESFMGTLVEFYDKPLGNEA 1093 >SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT |Schizosaccharomyces pombe|chr 2|||Manual Length = 509 Score = 25.0 bits (52), Expect = 8.3 Identities = 15/55 (27%), Positives = 27/55 (49%) Frame = -3 Query: 168 IGIHWRLDSLVILLFLTLSDGSILYRPS*LFFFSVITPWVETFIIIRFICAIHLV 4 IG+H D +++LLF D Y + F+ S PW+ +I+ + H++ Sbjct: 62 IGVHHLYDGVLVLLF----DALFTYFVA-AFYRSSRMPWIIFIVILGHTFSSHVI 111 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,292,852 Number of Sequences: 5004 Number of extensions: 45549 Number of successful extensions: 147 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 147 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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