Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20100
(553 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 109 2e-25
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 109 2e-25
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 104 1e-23
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 98 9e-22
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 71 1e-13
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 49 4e-07
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 40 3e-04
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 31 0.15
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.60
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 26 3.2
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 26 4.2
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 26 4.2
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 5.6
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 5.6
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 7.4
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 9.8
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 109 bits (263), Expect = 2e-25
Identities = 50/58 (86%), Positives = 54/58 (93%)
Frame = +2
Query: 86 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP 259
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 71.7 bits (168), Expect = 7e-14
Identities = 33/49 (67%), Positives = 41/49 (83%)
Frame = +3
Query: 363 GKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 509
GKP I+V +KGE KTF PEE+SSMVL KM+E+AEA+LG V +AV+TVP
Sbjct: 97 GKPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVP 145
Score = 66.9 bits (156), Expect = 2e-12
Identities = 29/57 (50%), Positives = 40/57 (70%)
Frame = +1
Query: 253 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVDSDEANLRSR*HIRVKTKPFSPRK 423
+P+NTIFDAKRLIGR+F D VQ+DMKHWPF+V + + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEE 116
Score = 33.9 bits (74), Expect = 0.016
Identities = 14/15 (93%), Positives = 15/15 (100%)
Frame = +2
Query: 509 AFFNDSQRQATKDAG 553
A+FNDSQRQATKDAG
Sbjct: 146 AYFNDSQRQATKDAG 160
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 109 bits (263), Expect = 2e-25
Identities = 50/58 (86%), Positives = 54/58 (93%)
Frame = +2
Query: 86 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP 259
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 75.8 bits (178), Expect = 4e-15
Identities = 35/49 (71%), Positives = 41/49 (83%)
Frame = +3
Query: 363 GKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 509
GKP ++V YKGE KTF PEE+SSMVL KM+ETAEAYLG V +AV+TVP
Sbjct: 97 GKPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVP 145
Score = 69.7 bits (163), Expect = 3e-13
Identities = 31/57 (54%), Positives = 42/57 (73%)
Frame = +1
Query: 253 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVDSDEANLRSR*HIRVKTKPFSPRK 423
+P+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V S + + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEE 116
Score = 33.9 bits (74), Expect = 0.016
Identities = 14/15 (93%), Positives = 15/15 (100%)
Frame = +2
Query: 509 AFFNDSQRQATKDAG 553
A+FNDSQRQATKDAG
Sbjct: 146 AYFNDSQRQATKDAG 160
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 104 bits (249), Expect = 1e-23
Identities = 60/156 (38%), Positives = 85/156 (54%), Gaps = 1/156 (0%)
Frame = +2
Query: 89 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP-TT 265
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P T
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 266 QYSMPNVSSDVSSKMLLCKPT*STGLSRLTVMRQT*DQGSI*G*RQNLFPRGSQFHGAYE 445
+ + + + + K S + + + ++ G ++ P +
Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSK 157
Query: 446 NEGNCRSLSWQNCAECSYHGSAFFNDSQRQATKDAG 553
+ + + A+FND+QRQATKDAG
Sbjct: 158 MKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAG 193
Score = 64.1 bits (149), Expect = 1e-11
Identities = 28/48 (58%), Positives = 39/48 (81%)
Frame = +3
Query: 366 KPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 509
+P ++V G+ K F PEE+S+M+L+KMK+TAEAYLGK V +AV+TVP
Sbjct: 131 RPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVP 178
Score = 53.6 bits (123), Expect = 2e-08
Identities = 24/57 (42%), Positives = 37/57 (64%)
Frame = +1
Query: 253 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVDSDEANLRSR*HIRVKTKPFSPRK 423
+P NTIFD KRLIGRKF++ T+ D+K +PF + +D+ ++ K K F+P +
Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEE 149
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 97.9 bits (233), Expect = 9e-22
Identities = 44/58 (75%), Positives = 50/58 (86%)
Frame = +2
Query: 86 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTP 259
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AM P
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65
Score = 60.5 bits (140), Expect = 2e-10
Identities = 30/49 (61%), Positives = 36/49 (73%)
Frame = +3
Query: 363 GKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 509
G P I+V Y GE K F +E+S+MVLTKMKE +EA L K V+ AVITVP
Sbjct: 101 GIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVP 149
Score = 58.0 bits (134), Expect = 9e-10
Identities = 22/33 (66%), Positives = 28/33 (84%)
Frame = +1
Query: 253 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFEV 351
+P NT+FDAKRLIGR++ED Q D+KHWPF+V
Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKV 96
Score = 29.5 bits (63), Expect = 0.34
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +2
Query: 509 AFFNDSQRQATKDAG 553
A+F+DSQR ATKDAG
Sbjct: 150 AYFSDSQRAATKDAG 164
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 70.9 bits (166), Expect = 1e-13
Identities = 33/66 (50%), Positives = 44/66 (66%), Gaps = 1/66 (1%)
Frame = +2
Query: 77 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 253
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 254 TPTTQY 271
P +
Sbjct: 109 NPENTF 114
Score = 50.8 bits (116), Expect = 1e-07
Identities = 20/36 (55%), Positives = 28/36 (77%)
Frame = +3
Query: 402 KTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 509
KT+ P ++ +L+KM+ETA YLGK V+NAV+TVP
Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVP 191
Score = 41.1 bits (92), Expect = 1e-04
Identities = 16/33 (48%), Positives = 24/33 (72%)
Frame = +1
Query: 253 DPNNTIFDAKRLIGRKFEDATVQADMKHWPFEV 351
+P NT F KRLIGR+F++ VQ D+K P+++
Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKI 141
Score = 30.7 bits (66), Expect = 0.15
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +2
Query: 509 AFFNDSQRQATKDAG 553
A+FNDSQRQATK AG
Sbjct: 192 AYFNDSQRQATKAAG 206
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 49.2 bits (112), Expect = 4e-07
Identities = 20/59 (33%), Positives = 38/59 (64%)
Frame = +2
Query: 74 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 250
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 39.5 bits (88), Expect = 3e-04
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +2
Query: 89 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQV 247
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQL 79
Score = 27.5 bits (58), Expect = 1.4
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +3
Query: 396 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 509
++K E S L ++ E+AE +LG V V++VP
Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVP 171
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 30.7 bits (66), Expect = 0.15
Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +2
Query: 89 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMTPTT 265
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83
Query: 266 QYSMPNV 286
+S+ NV
Sbjct: 84 -HSIRNV 89
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.60
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 81 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 176
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 26.2 bits (55), Expect = 3.2
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +2
Query: 185 PSYVAFTDTERLIGDAAKNQVAMTPTTQYSMPNVSSDVSSKMLLC 319
P +A T ER++ AAK A+ T Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.8 bits (54), Expect = 4.2
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +2
Query: 434 GAYENEGNCRSLSWQNCAECSYHGSAFFND 523
GAY N C +S N A CS SA ND
Sbjct: 1689 GAYSNA--CVDISLSNLALCSILNSALIND 1716
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 25.8 bits (54), Expect = 4.2
Identities = 11/44 (25%), Positives = 24/44 (54%)
Frame = -3
Query: 356 LSTSKGQCFMSACTVASSNLRPMRRLASNIVLLGSSPPGSWRHL 225
L+ S+ + ++S V +RP + + +IV+ GS++H+
Sbjct: 1173 LNASQAEAYLSKMQVGDLVIRPSSKGSDHIVVTWKVAEGSYQHI 1216
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 5.6
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 197 AFTDTERLIGDAAKNQVAMTPTTQYSMPNVSSDV 298
AF D + G AK A P+T+ + N+S V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.4 bits (53), Expect = 5.6
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -3
Query: 167 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 63
G W SP S + H ++++ +F +RVL C+
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 25.0 bits (52), Expect = 7.4
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +1
Query: 67 QNGKSTRSRNRSGYHVLLRWCLPARE 144
+NGK T +R +S + W P++E
Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE 502
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 24.6 bits (51), Expect = 9.8
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +2
Query: 92 GIDLGTTYSCVGVFQHGKVEIIANDQGNR 178
G LG TY+ V Q G VE+ D N+
Sbjct: 1279 GCFLGNTYAFASVTQDGSVEVHRLDVNNQ 1307
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,450,321
Number of Sequences: 5004
Number of extensions: 52742
Number of successful extensions: 193
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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