BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20096 (679 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|c... 122 4e-29 SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||M... 93 3e-20 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 50 3e-07 SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 42 6e-05 SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosacch... 36 0.004 SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 35 0.012 SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 34 0.022 SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 33 0.029 SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 31 0.20 SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 31 0.20 SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pomb... 28 1.4 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 28 1.4 SPCC4E9.01c |rec11|SPCC550.16c|meiotic cohesin complex subunit R... 27 1.9 SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 27 3.3 SPBC1347.08c |||ribonuclease H2 complex subunit|Schizosaccharomy... 26 4.4 SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein... 26 4.4 SPCC188.12 |spn6|SPCC584.09|septin Spn6|Schizosaccharomyces pomb... 26 5.8 SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|c... 26 5.8 SPBC2D10.15c |||peptidyl-tRNA hydrolase Pth1 |Schizosaccharomyce... 26 5.8 SPAC9E9.10c |cbh1|cbh|centromere binding protein |Schizosaccharo... 26 5.8 SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 25 7.6 SPAC4G8.13c |prz1||transcription factor Prz1 |Schizosaccharomyce... 25 7.6 SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|S... 25 7.6 >SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 392 Score = 122 bits (295), Expect = 4e-29 Identities = 65/148 (43%), Positives = 98/148 (66%), Gaps = 6/148 (4%) Frame = +1 Query: 253 RSKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIFNLCRAFDDEDVIHVDGDVN 432 +S+VPAFL V DIAGL +GA+ G GLGNAFLSH++A DAI+ + RAFDD ++IHV+GDV+ Sbjct: 80 KSRVPAFLTVFDIAGLTKGASTGVGLGNAFLSHVRAVDAIYQVVRAFDDAEIIHVEGDVD 139 Query: 433 PVRDLETIAEELRLKDEEQLLQHIENWTEWSTEAAKRNLNLNTIPWLKSRL-----FLVD 597 P+RDL I +EL +KD E + +H+E + ++ A L + ++ + +L + Sbjct: 140 PIRDLSIIVDELLIKDAEFVEKHLEGLRKITSRGA-NTLEMKAKKEEQAIIEKVYQYLTE 198 Query: 598 EKKHIRFGDWSALILK-FK*IPVLTSKP 678 K+ IR GDWS ++ + +LT+KP Sbjct: 199 TKQPIRKGDWSNREVEIINSLYLLTAKP 226 Score = 94.3 bits (224), Expect = 1e-20 Identities = 41/68 (60%), Positives = 53/68 (77%), Gaps = 1/68 (1%) Frame = +2 Query: 62 GRVGTNLKVGIVGVPNVGKSTFFNVLTKSQAA-AENFPFCTIDPNESRVPVPDERYDYLC 238 GR G NLK GIVG+PNVGKSTFF +TKS N+P+ TIDP E++V VPDER+D+LC Sbjct: 15 GRPGNNLKTGIVGMPNVGKSTFFRAITKSVLGNPANYPYATIDPEEAKVAVPDERFDWLC 74 Query: 239 EYHKPEAK 262 E +KP+++ Sbjct: 75 EAYKPKSR 82 >SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 407 Score = 93.5 bits (222), Expect = 3e-20 Identities = 43/85 (50%), Positives = 63/85 (74%) Frame = +1 Query: 253 RSKVPAFLNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIFNLCRAFDDEDVIHVDGDVN 432 ++++PA L + D AGL R +++G+GLGNAFLS+I++ DA+F L RAF + V HV+ V+ Sbjct: 105 KTRIPAQLTIYDTAGLTRNSSKGEGLGNAFLSNIRSVDALFQLVRAFPEAQVPHVEKSVD 164 Query: 433 PVRDLETIAEELRLKDEEQLLQHIE 507 PVRDL+ I EEL LKD E L +++ Sbjct: 165 PVRDLQIIQEELLLKDAEFLESYLK 189 Score = 82.2 bits (194), Expect = 6e-17 Identities = 34/69 (49%), Positives = 50/69 (72%), Gaps = 1/69 (1%) Frame = +2 Query: 59 IGRVGTNLKVGIVGVPNVGKSTFFNVLTKSQAA-AENFPFCTIDPNESRVPVPDERYDYL 235 +GR G +LK+GIVG+PN+GKST F +LTK+ N+PF TIDP ++ PV D +Y+ L Sbjct: 39 LGRPGNHLKIGIVGMPNIGKSTLFQILTKTNLGNPANYPFATIDPVHAKAPVLDSQYELL 98 Query: 236 CEYHKPEAK 262 CE ++P+ + Sbjct: 99 CEIYQPKTR 107 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 50.0 bits (114), Expect = 3e-07 Identities = 21/44 (47%), Positives = 29/44 (65%) Frame = +2 Query: 68 VGTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTIDPNES 199 +G ++ +G VG P+ GKST N LT + A NFPF TI+PN + Sbjct: 1 MGRDILIGFVGKPSSGKSTMLNALTDATAKTGNFPFTTIEPNRA 44 Score = 39.1 bits (87), Expect = 6e-04 Identities = 15/36 (41%), Positives = 26/36 (72%) Frame = +1 Query: 274 LNVVDIAGLVRGAAEGQGLGNAFLSHIKACDAIFNL 381 + ++D+AGL+ GA G+GLGN FL ++ DA+ ++ Sbjct: 79 IQLLDVAGLIPGAHAGKGLGNKFLDDLRHADALVHV 114 >SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 42.3 bits (95), Expect = 6e-05 Identities = 20/55 (36%), Positives = 31/55 (56%), Gaps = 2/55 (3%) Frame = +2 Query: 74 TNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTIDP--NESRVPVPDERYDY 232 T ++G+VG+PN GKST N LT S++ + F TI P + +PD+ + Sbjct: 235 TICEIGLVGLPNAGKSTLLNCLTASKSKVGEYEFTTIYPKIGTIKTTMPDDHSSF 289 Score = 34.7 bits (76), Expect = 0.012 Identities = 12/26 (46%), Positives = 21/26 (80%) Frame = +1 Query: 280 VVDIAGLVRGAAEGQGLGNAFLSHIK 357 + DI G+++GA++G+GLG FL H++ Sbjct: 293 LADIPGIIKGASDGKGLGYDFLRHVE 318 >SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 36.3 bits (80), Expect = 0.004 Identities = 13/34 (38%), Positives = 24/34 (70%) Frame = +2 Query: 83 KVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTI 184 +V +G P+VGKST + +TK+++A ++ F T+ Sbjct: 64 RVAFIGFPSVGKSTLLSAITKTKSATASYEFTTL 97 >SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 34.7 bits (76), Expect = 0.012 Identities = 14/36 (38%), Positives = 22/36 (61%) Frame = +2 Query: 65 RVGTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFP 172 ++ ++L VG++G PNVGKS+ N L A + P Sbjct: 265 KLKSSLTVGVIGYPNVGKSSVINALVNRSANGRSAP 300 >SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 33.9 bits (74), Expect = 0.022 Identities = 13/30 (43%), Positives = 20/30 (66%) Frame = +2 Query: 92 IVGVPNVGKSTFFNVLTKSQAAAENFPFCT 181 + G PNVGKS+F N +T++Q + + F T Sbjct: 173 VCGYPNVGKSSFMNKVTRAQVDVQPYAFTT 202 >SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 366 Score = 33.5 bits (73), Expect = 0.029 Identities = 16/42 (38%), Positives = 24/42 (57%) Frame = +2 Query: 59 IGRVGTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCTI 184 + R G VG +G P+VGKST LT +++ A + F T+ Sbjct: 58 VARTGIGT-VGFIGFPSVGKSTLMTQLTGTRSEAAAYEFTTL 98 >SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 30.7 bits (66), Expect = 0.20 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +2 Query: 80 LKVGIVGVPNVGKSTFFNVLTKSQ 151 + VG++G PN GKS+ N L K + Sbjct: 311 ISVGLIGFPNAGKSSIINTLRKKK 334 >SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual Length = 616 Score = 30.7 bits (66), Expect = 0.20 Identities = 13/26 (50%), Positives = 17/26 (65%) Frame = +2 Query: 74 TNLKVGIVGVPNVGKSTFFNVLTKSQ 151 T + G+VG PNVGKS+ N L S+ Sbjct: 300 TKMTFGLVGYPNVGKSSTINALVGSK 325 >SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pombe|chr 1|||Manual Length = 431 Score = 27.9 bits (59), Expect = 1.4 Identities = 19/72 (26%), Positives = 38/72 (52%), Gaps = 9/72 (12%) Frame = +1 Query: 343 LSHIKACDAIFNLCRAFDDED-------VIHVDGD--VNPVRDLETIAEELRLKDEEQLL 495 L+ ++A + ++C FD+ED + VDGD + + D E + ++ L LL Sbjct: 58 LARVRAMKRLVSICSNFDEEDNWNGVWNTLVVDGDSHASAMIDYEGLLDKCSL--SRNLL 115 Query: 496 QHIENWTEWSTE 531 + I ++ E++T+ Sbjct: 116 ELINDYAEYTTQ 127 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 27.9 bits (59), Expect = 1.4 Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 4/41 (9%) Frame = +2 Query: 83 KVGIVGVPNVGKSTFFNVLTKSQAA----AENFPFCTIDPN 193 KV IVG+ GKST +L K+ + + +P ID N Sbjct: 1130 KVAIVGISGSGKSTLVELLRKTYPSEDIYIDGYPLTNIDTN 1170 >SPCC4E9.01c |rec11|SPCC550.16c|meiotic cohesin complex subunit Rec11|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 27.5 bits (58), Expect = 1.9 Identities = 9/35 (25%), Positives = 23/35 (65%) Frame = +1 Query: 442 DLETIAEELRLKDEEQLLQHIENWTEWSTEAAKRN 546 D+ ++L+L +EE++++ IE + WS + +++ Sbjct: 837 DINFSDDDLKLMNEEEIMEQIETFKAWSMKFKEKS 871 >SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 26.6 bits (56), Expect = 3.3 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +2 Query: 77 NLKVGIVGVPNVGKSTFFNVLTKSQAA 157 ++K+ IVG NVG +T F +L AA Sbjct: 20 SIKIVIVGAGNVGSTTAFTLLLSGLAA 46 >SPBC1347.08c |||ribonuclease H2 complex subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 293 Score = 26.2 bits (55), Expect = 4.4 Identities = 15/58 (25%), Positives = 26/58 (44%), Gaps = 8/58 (13%) Frame = +2 Query: 134 VLTKSQAAAENFPFCTID-------PNESRVPVPDERYDYLCEYHKPEAKCQ-LSSTW 283 +L K++ A EN P + P + R P+P + + C++H C+ L W Sbjct: 155 LLRKAKVAQENLPPSIVTELKKQLAPLDLRTPLPQDLLELSCKWHAASLVCEDLQPEW 212 >SPBC1706.03 |fzo1|SPBC839.01|mitochondrial fusion GTPase protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 758 Score = 26.2 bits (55), Expect = 4.4 Identities = 17/43 (39%), Positives = 20/43 (46%), Gaps = 1/43 (2%) Frame = +2 Query: 56 LIGRV-GTNLKVGIVGVPNVGKSTFFNVLTKSQAAAENFPFCT 181 L RV T+ K+ I G N GKST N L E+ CT Sbjct: 146 LFSRVEDTSSKILITGDLNAGKSTLCNALVHKDILPEDQQPCT 188 >SPCC188.12 |spn6|SPCC584.09|septin Spn6|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 25.8 bits (54), Expect = 5.8 Identities = 12/28 (42%), Positives = 15/28 (53%) Frame = +2 Query: 56 LIGRVGTNLKVGIVGVPNVGKSTFFNVL 139 LI R L + + G GK+TFFN L Sbjct: 23 LIKRKECGLTIMLCGASGTGKTTFFNTL 50 >SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 433 Score = 25.8 bits (54), Expect = 5.8 Identities = 15/39 (38%), Positives = 22/39 (56%) Frame = +1 Query: 328 LGNAFLSHIKACDAIFNLCRAFDDEDVIHVDGDVNPVRD 444 LG + S KA DAI L + F+D D V ++NP+ + Sbjct: 196 LGFSSSSKPKAVDAIVKLYKVFNDCDATQV--EINPLAE 232 >SPBC2D10.15c |||peptidyl-tRNA hydrolase Pth1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 218 Score = 25.8 bits (54), Expect = 5.8 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = -3 Query: 389 ARHKLKIASQALMWDRKAFPNPWPSAAPRTRPAMSTT 279 +RH L ++ D AFP WPS +ST+ Sbjct: 46 SRHSLGKLMVSMYADSMAFPKNWPSTKENLTLVLSTS 82 >SPAC9E9.10c |cbh1|cbh|centromere binding protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 514 Score = 25.8 bits (54), Expect = 5.8 Identities = 16/67 (23%), Positives = 32/67 (47%), Gaps = 3/67 (4%) Frame = +1 Query: 433 PVRDLETIAEELRLKDEEQLLQHI---ENWTEWSTEAAKRNLNLNTIPWLKSRLFLVDEK 603 P+R ++AE+ ++D H + TEW + ++L+ +TI + S + + Sbjct: 3 PIRQAVSLAEKKAIRDYYNQSAHRIPQKEVTEWFRKTYNKDLSQSTISEILSPKYSYLDD 62 Query: 604 KHIRFGD 624 +R GD Sbjct: 63 GSVRLGD 69 >SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 844 Score = 25.4 bits (53), Expect = 7.6 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +1 Query: 445 LETIAEELRLKDEEQLLQHIENWTEWSTEAAKRNLN 552 LE EE+R +++ + Q N T + E +RN+N Sbjct: 110 LEQRLEEIRKRNQSRKNQMSNNSTTYHRETKRRNIN 145 >SPAC4G8.13c |prz1||transcription factor Prz1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 681 Score = 25.4 bits (53), Expect = 7.6 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = -1 Query: 595 QLGIVLTLARESYSGLSFFSPPLLTTRSSSRYAAEAAP 482 Q+ +V + +S SG S + PLL T S Y+ A+P Sbjct: 411 QINVVPSSPSKSQSGPSLPANPLLQTDISITYSQSASP 448 >SPCC417.06c |ppk35|mug27|serine/threonine protein kinase Ppk35|Schizosaccharomyces pombe|chr 3|||Manual Length = 624 Score = 25.4 bits (53), Expect = 7.6 Identities = 20/86 (23%), Positives = 34/86 (39%) Frame = +1 Query: 370 IFNLCRAFDDEDVIHVDGDVNPVRDLETIAEELRLKDEEQLLQHIENWTEWSTEAAKRNL 549 + L AF D++ +++ + P D T L E Q ++ + K Sbjct: 222 LVKLYYAFQDKEKVYLAMEYVPGGDFRTFLTTKGLLHENQTRFYLAEMVAAISAVHKLGY 281 Query: 550 NLNTIPWLKSRLFLVDEKKHIRFGDW 627 LK FL+D+K HI+ D+ Sbjct: 282 MHRD---LKPENFLIDQKGHIKLSDF 304 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,480,368 Number of Sequences: 5004 Number of extensions: 48542 Number of successful extensions: 202 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 188 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 200 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 311890690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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