Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20088
(701 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 271 3e-73
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 271 3e-73
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 271 3e-73
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 271 3e-73
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 156 2e-38
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 153 8e-38
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 89 2e-18
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 82 4e-16
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 50 1e-06
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 50 1e-06
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 47 1e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 45 4e-05
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 44 1e-04
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 42 4e-04
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 40 0.002
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 38 0.009
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 38 0.009
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 33 0.14
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 33 0.14
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 33 0.18
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.24
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 33 0.24
At5g13650.2 68418.m01585 elongation factor family protein contai... 32 0.32
At5g13650.1 68418.m01584 elongation factor family protein contai... 32 0.32
At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 30 1.7
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.7
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.7
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.7
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.3
At2g20850.1 68415.m02457 leucine-rich repeat protein kinase, put... 29 3.9
At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containi... 28 5.2
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.2
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 5.2
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.9
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 9.1
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 9.1
At3g19510.1 68416.m02472 homeobox protein (HAT 3.1) identical to... 27 9.1
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 271 bits (665), Expect = 3e-73
Identities = 129/149 (86%), Positives = 136/149 (91%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 415
DKLKAE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 416 GEFEAGISKNGQTREHALLAFTLGVKQLI 502
G FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 94.3 bits (224), Expect = 7e-20
Identities = 39/61 (63%), Positives = 50/61 (81%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFK 690
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+K
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYK 212
Query: 691 G 693
G
Sbjct: 213 G 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 271 bits (665), Expect = 3e-73
Identities = 129/149 (86%), Positives = 136/149 (91%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 415
DKLKAE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 416 GEFEAGISKNGQTREHALLAFTLGVKQLI 502
G FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 94.3 bits (224), Expect = 7e-20
Identities = 39/61 (63%), Positives = 50/61 (81%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFK 690
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+K
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYK 212
Query: 691 G 693
G
Sbjct: 213 G 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 271 bits (665), Expect = 3e-73
Identities = 129/149 (86%), Positives = 136/149 (91%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 415
DKLKAE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 416 GEFEAGISKNGQTREHALLAFTLGVKQLI 502
G FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 94.3 bits (224), Expect = 7e-20
Identities = 39/61 (63%), Positives = 50/61 (81%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFK 690
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+K
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYK 212
Query: 691 G 693
G
Sbjct: 213 G 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 271 bits (665), Expect = 3e-73
Identities = 129/149 (86%), Positives = 136/149 (91%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 236 DKLKAELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 415
DKLKAE GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 416 GEFEAGISKNGQTREHALLAFTLGVKQLI 502
G FEAGISK+GQTREHALLAFTLGVKQ+I
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMI 149
Score = 94.3 bits (224), Expect = 7e-20
Identities = 39/61 (63%), Positives = 50/61 (81%)
Frame = +1
Query: 511 NKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFK 690
NKMD+T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+K
Sbjct: 153 NKMDATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYK 212
Query: 691 G 693
G
Sbjct: 213 G 213
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 156 bits (378), Expect = 2e-38
Identities = 70/150 (46%), Positives = 103/150 (68%), Gaps = 1/150 (0%)
Frame = +2
Query: 71 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 250
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 251 ELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 430
E GIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 431 GISK-NGQTREHALLAFTLGVKQLIVG*TK 517
G GQTREHA + GV+Q+IV K
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINK 387
Score = 38.3 bits (85), Expect = 0.005
Identities = 20/65 (30%), Positives = 37/65 (56%), Gaps = 3/65 (4%)
Frame = +1
Query: 508 INKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EPSTK--M 678
INKMD YS+ RF+ IK+ V S+++ + +++ ++P+S N++ PS
Sbjct: 385 INKMDIVG--YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNRLS 442
Query: 679 PWFKG 693
W++G
Sbjct: 443 SWYQG 447
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 153 bits (372), Expect = 8e-38
Identities = 70/147 (47%), Positives = 101/147 (68%)
Frame = +2
Query: 65 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 244
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 245 KAELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 424
+ E G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 425 EAGISKNGQTREHALLAFTLGVKQLIV 505
E G + GQTREH LA TLGV +LIV
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIV 244
Score = 46.8 bits (106), Expect = 1e-05
Identities = 24/65 (36%), Positives = 39/65 (60%), Gaps = 3/65 (4%)
Frame = +1
Query: 508 INKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMLEPSTK--M 678
+NKMD +S+ R++EI++++ ++K GYN V F+PISG G NM + +
Sbjct: 246 VNKMDDPTVNWSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEIC 305
Query: 679 PWFKG 693
PW+ G
Sbjct: 306 PWWSG 310
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 89.4 bits (212), Expect = 2e-18
Identities = 53/151 (35%), Positives = 78/151 (51%)
Frame = +2
Query: 53 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 232
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 233 LDKLKAELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 412
+D E + GITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 413 TGEFEAGISKNGQTREHALLAFTLGVKQLIV 505
G QT+EH LLA +GV ++V
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVV 200
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 81.8 bits (193), Expect = 4e-16
Identities = 53/147 (36%), Positives = 73/147 (49%)
Frame = +2
Query: 62 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 241
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 242 LKAELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 421
E GITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 422 FEAGISKNGQTREHALLAFTLGVKQLI 502
QT+EH LLA +GV L+
Sbjct: 168 MP-------QTKEHILLARQVGVPSLV 187
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 50.4 bits (115), Expect = 1e-06
Identities = 44/151 (29%), Positives = 65/151 (43%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAELS 259
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + E
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 260 VGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 439
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 440 KNGQTREHALLAFTLGVKQLIVG*TKWIPLN 532
+ Q R + + K +G W LN
Sbjct: 177 VDRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 50.4 bits (115), Expect = 1e-06
Identities = 44/151 (29%), Positives = 65/151 (43%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAELS 259
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + E
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 260 VGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 439
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 440 KNGQTREHALLAFTLGVKQLIVG*TKWIPLN 532
+ Q R + + K +G W LN
Sbjct: 177 VDRQMRRYEVPRVAFINKLDRMGADPWKVLN 207
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 47.2 bits (107), Expect = 1e-05
Identities = 35/113 (30%), Positives = 51/113 (45%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAELS 259
NI ++ HVD GK+T HLI GG + GK F +D L E
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 260 VGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 45.2 bits (102), Expect = 4e-05
Identities = 33/117 (28%), Positives = 53/117 (45%)
Frame = +2
Query: 68 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 247
K + NI ++ H+D+GK+TTT ++Y G + E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGR---------NYKIGEVHEGTATMDW----ME 140
Query: 248 AELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
E GITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 43.6 bits (98), Expect = 1e-04
Identities = 41/132 (31%), Positives = 54/132 (40%), Gaps = 7/132 (5%)
Frame = +2
Query: 44 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 223
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 224 AWVLDKLKAELSVGITIDIALWKFET-------SKYYVTIIDAPGHRDFIKNMITGTSQA 382
LDKL+ E + + A +E S Y + +ID PGH DF + S
Sbjct: 103 ---LDKLQRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSAC 159
Query: 383 DCAVLIVAAGTG 418
A+L+V A G
Sbjct: 160 QGALLVVDAAQG 171
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 41.9 bits (94), Expect = 4e-04
Identities = 33/117 (28%), Positives = 51/117 (43%), Gaps = 4/117 (3%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAELS 259
N +I H+D GKST L+ G + R + K F LD + E
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 260 VGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
GITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.9 bits (89), Expect = 0.002
Identities = 31/112 (27%), Positives = 53/112 (47%), Gaps = 4/112 (3%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 247
N+ VI HVD GKST T L+ G I + + + A E +G + K + L
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGI--SLY 78
Query: 248 AELSVGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 403
E++ ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 79 YEMT-DESLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 37.5 bits (83), Expect = 0.009
Identities = 31/115 (26%), Positives = 50/115 (43%), Gaps = 2/115 (1%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV-LDKLKAEL 256
N+ ++GH+ GK+ L+ + + K EK KY +D+ + +
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYTDTRVDEQERNI 190
Query: 257 SV-GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
S+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 191 SIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 37.5 bits (83), Expect = 0.009
Identities = 31/115 (26%), Positives = 50/115 (43%), Gaps = 2/115 (1%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV-LDKLKAEL 256
N+ ++GH+ GK+ L+ + + K EK KY +D+ + +
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYTDTRVDEQERNI 190
Query: 257 SV-GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
S+ + + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 191 SIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 33.5 bits (73), Expect = 0.14
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +2
Query: 299 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 478
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 479 TLGVKQLIV 505
+ +K +I+
Sbjct: 178 MMRLKHIII 186
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 33.5 bits (73), Expect = 0.14
Identities = 19/69 (27%), Positives = 33/69 (47%)
Frame = +2
Query: 299 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 478
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 479 TLGVKQLIV 505
+ +K +I+
Sbjct: 178 MMRLKHIII 186
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 33.1 bits (72), Expect = 0.18
Identities = 22/75 (29%), Positives = 35/75 (46%), Gaps = 4/75 (5%)
Frame = +2
Query: 293 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 460
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 461 HALLAFTLGVKQLIV 505
H + +K +I+
Sbjct: 166 HLAAVEIMQLKHIII 180
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.24
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +2
Query: 314 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 32.7 bits (71), Expect = 0.24
Identities = 19/65 (29%), Positives = 31/65 (47%)
Frame = +2
Query: 311 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 490
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 491 KQLIV 505
K +I+
Sbjct: 174 KDIII 178
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 32.3 bits (70), Expect = 0.32
Identities = 30/113 (26%), Positives = 46/113 (40%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAELS 259
NI ++ HVD GK+T ++ + K ++ Q M + ++D E
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 260 VGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
GITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 182
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 32.3 bits (70), Expect = 0.32
Identities = 30/113 (26%), Positives = 46/113 (40%)
Frame = +2
Query: 80 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAELS 259
NI ++ HVD GK+T ++ + K ++ Q M + ++D E
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 260 VGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 418
GITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 181
>At5g64600.1 68418.m08118 expressed protein similar to axi 1
[Nicotiana tabacum] GI:559921; contains Pfam profile
PF03138: Plant protein family
Length = 522
Score = 29.9 bits (64), Expect = 1.7
Identities = 19/68 (27%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
Frame = +1
Query: 484 RCQTAHRRINKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG--DNM 657
R T+H +I ++STE E KEV ++K +GY+ + V ++ +G D +
Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYGGDDRL 371
Query: 658 LEPSTKMP 681
E ++ P
Sbjct: 372 SELKSRFP 379
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 320 IIDAPGHRDFIKNMITGTSQADCAVLIV 403
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 320 IIDAPGHRDFIKNMITGTSQADCAVLIV 403
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 320 IIDAPGHRDFIKNMITGTSQADCAVLIV 403
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.3
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2
Query: 314 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 403
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At2g20850.1 68415.m02457 leucine-rich repeat protein kinase,
putative contains Pfam domains PF00560: Leucine Rich
Repeat and PF00069: Protein kinase domain
Length = 772
Score = 28.7 bits (61), Expect = 3.9
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = +2
Query: 236 DKLKAELSVGITIDIALWKFETSKYYVTIIDAPG-HRDF 349
D+LK ELS + + IAL + +Y I D P HR+F
Sbjct: 566 DRLKIELSWNVRVRIALEAAKALEYLHEICDPPSIHRNF 604
>At3g43240.1 68416.m04564 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 747
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +3
Query: 594 DWLQPSCCRFRAHFWMARRQHVGAFNQNALVQGM 695
DW+ C AHF RR +GAF A G+
Sbjct: 687 DWVNCGSCGEWAHFGCDRRPGLGAFKDYAKTDGL 720
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +1
Query: 541 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 630
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 28.3 bits (60), Expect = 5.2
Identities = 19/64 (29%), Positives = 26/64 (40%), Gaps = 4/64 (6%)
Frame = +2
Query: 239 KLKAELSVGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 406
K+ A + GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 407 AGTG 418
A G
Sbjct: 585 ADDG 588
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 6.9
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +1
Query: 547 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 657
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 9.1
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +1
Query: 523 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 636
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 9.1
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +1
Query: 523 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 636
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At3g19510.1 68416.m02472 homeobox protein (HAT 3.1) identical to
homeotic protein HAT 3.1 (GI:11994474) [Arabidopsis
thaliana]
Length = 723
Score = 27.5 bits (58), Expect = 9.1
Identities = 10/52 (19%), Positives = 25/52 (48%)
Frame = +1
Query: 502 RRINKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 657
++ NK + + + IKK++ ++ +I Y + + + GW G ++
Sbjct: 160 KKKNKTMNKGQVREDDEYTRIKKKLRYFLNRINYEQSLIDAYSLEGWKGSSL 211
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,690,038
Number of Sequences: 28952
Number of extensions: 332005
Number of successful extensions: 1014
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 966
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1005
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1506636208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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