BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20086
(679 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g66100.1 68418.m08327 La domain-containing protein similar to... 36 0.019
At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing ... 34 0.099
At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing ... 34 0.099
At5g21160.1 68418.m02528 La domain-containing protein / proline-... 33 0.23
At4g32720.1 68417.m04657 RNA recognition motif (RRM)-containing ... 31 0.70
At2g41060.1 68415.m05070 RNA recognition motif (RRM)-containing ... 31 0.93
At1g79880.1 68414.m09333 La domain-containing protein contains P... 30 1.2
At2g43970.2 68415.m05468 La domain-containing protein contains P... 29 2.1
At2g43970.1 68415.m05467 La domain-containing protein contains P... 29 2.1
At1g62010.1 68414.m06994 mitochondrial transcription termination... 29 2.8
At3g19090.1 68416.m02426 RNA-binding protein, putative similar t... 29 3.7
At2g19380.1 68415.m02260 RNA recognition motif (RRM)-containing ... 29 3.7
At1g52620.1 68414.m05941 pentatricopeptide (PPR) repeat-containi... 28 4.9
At1g22450.1 68414.m02806 cytochrome c oxidase subunit 6b, putati... 28 4.9
At4g11690.1 68417.m01867 pentatricopeptide (PPR) repeat-containi... 28 6.5
At1g53650.1 68414.m06105 RNA-binding protein, putative similar t... 28 6.5
At5g40870.1 68418.m04963 uracil phosphoribosyltransferase, putat... 27 8.6
At5g15430.1 68418.m01806 calmodulin-binding protein-related has ... 27 8.6
At5g01370.1 68418.m00050 expressed protein 27 8.6
>At5g66100.1 68418.m08327 La domain-containing protein similar to
SP|P40796 La protein homolog (La ribonucleoprotein) (La
autoantigen homolog) {Drosophila melanogaster}; contains
Pfam profile PF05383: La domain
Length = 453
Score = 36.3 bits (80), Expect = 0.019
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +2
Query: 260 QVEYYFGDVNLHRDKFLQEQIKLDDGWV 343
QVEYYF NL RD+ L++Q+ D+GWV
Sbjct: 342 QVEYYFSADNLSRDEHLRDQMN-DEGWV 368
>At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 420
Score = 33.9 bits (74), Expect = 0.099
Identities = 15/23 (65%), Positives = 16/23 (69%)
Frame = +2
Query: 257 RQVEYYFGDVNLHRDKFLQEQIK 325
RQVEYYF D NL DKFL +K
Sbjct: 109 RQVEYYFSDENLPTDKFLLNAMK 131
>At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 422
Score = 33.9 bits (74), Expect = 0.099
Identities = 15/23 (65%), Positives = 16/23 (69%)
Frame = +2
Query: 257 RQVEYYFGDVNLHRDKFLQEQIK 325
RQVEYYF D NL DKFL +K
Sbjct: 109 RQVEYYFSDENLPTDKFLLNAMK 131
>At5g21160.1 68418.m02528 La domain-containing protein /
proline-rich family protein contains proline-rich
extensin domains, INTERPRO:IPR002965, PF05383: La domain
Length = 826
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +2
Query: 257 RQVEYYFGDVNLHRDKFLQEQIKLDDGWV 343
+QVEYYF D NL D +L + ++GWV
Sbjct: 284 KQVEYYFSDENLENDHYLISLMD-EEGWV 311
>At4g32720.1 68417.m04657 RNA recognition motif (RRM)-containing
protein RNA-binding protein LAH1, Saccharomyces
cerevisiae, PIR2:B48600; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 433
Score = 31.1 bits (67), Expect = 0.70
Identities = 15/30 (50%), Positives = 21/30 (70%), Gaps = 1/30 (3%)
Frame = +2
Query: 257 RQVEYYFGDVNLHRDKFLQEQI-KLDDGWV 343
RQVE+YF D NL D FL++ + + +DG V
Sbjct: 16 RQVEFYFSDSNLPIDDFLKKTVTESEDGLV 45
>At2g41060.1 68415.m05070 RNA recognition motif (RRM)-containing
protein similar to UBP1 interacting protein 1a
[Arabidopsis thaliana] GI:19574236; contains InterPro
entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM)
Length = 451
Score = 30.7 bits (66), Expect = 0.93
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = +3
Query: 516 KEICNRSIYAKGFAKDASLDDLLNYFKQFQEVEN 617
+++ +R I+ G D D L++ FKQ+ E+E+
Sbjct: 123 EDLVHRKIFVHGLGWDTKADSLIDAFKQYGEIED 156
>At1g79880.1 68414.m09333 La domain-containing protein contains Pfam
profile PF05383: La domain; similar to putative protein
GB:CAA18589 [Arabidopsis thaliana]
Length = 399
Score = 30.3 bits (65), Expect = 1.2
Identities = 15/29 (51%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +2
Query: 260 QVEYYFGDVNLHRDKFLQEQI-KLDDGWV 343
QVE+YF D NL D FL ++ K DG V
Sbjct: 16 QVEFYFSDSNLPTDGFLNREVTKSKDGLV 44
>At2g43970.2 68415.m05468 La domain-containing protein contains Pfam
profile PF05383: La domain
Length = 529
Score = 29.5 bits (63), Expect = 2.1
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +1
Query: 343 PLEILTKFNRLAKLTEDTDVIANALNKSTSGLLEVSEDNKKVRR 474
P+ ++ F ++ + + +A L S L VSED KKVRR
Sbjct: 229 PIHVVASFKKIKAVINNNSQLAAVLQNSAK--LFVSEDGKKVRR 270
>At2g43970.1 68415.m05467 La domain-containing protein contains Pfam
profile PF05383: La domain
Length = 545
Score = 29.5 bits (63), Expect = 2.1
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +1
Query: 343 PLEILTKFNRLAKLTEDTDVIANALNKSTSGLLEVSEDNKKVRR 474
P+ ++ F ++ + + +A L S L VSED KKVRR
Sbjct: 229 PIHVVASFKKIKAVINNNSQLAAVLQNSAK--LFVSEDGKKVRR 270
>At1g62010.1 68414.m06994 mitochondrial transcription termination
factor-related / mTERF-related contains Pfam profile
PF02536: mTERF
Length = 415
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/54 (31%), Positives = 29/54 (53%)
Frame = -3
Query: 506 SFISGSGCSGLRLTFLLSSETSNNPLVDLLRAFAMTSVSSVSLARRLNFVRISN 345
+F S S LRL L+ + +++ ++DLLR++ T S+ R + V I N
Sbjct: 48 TFKSSSFLDSLRLATKLTDKGNSDSVLDLLRSYGFTDSQISSIIRSDSRVLIDN 101
>At3g19090.1 68416.m02426 RNA-binding protein, putative similar to
RNA-binding protein homolog GB:AAF00075 GI:6449448 from
[Brassica napus]; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 455
Score = 28.7 bits (61), Expect = 3.7
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = +1
Query: 343 PLEILTKFNRLAKLTEDTDVIANALNKSTSGLLEVSEDNKKVRRNPE 483
P+ + ++ LT + +++ AL S+ L VSED KKV+R +
Sbjct: 180 PVSYIASTKKIKALTSNHHLVSLALRSSSK--LVVSEDGKKVKRTSQ 224
>At2g19380.1 68415.m02260 RNA recognition motif (RRM)-containing
protein similar to UBP1 interacting protein 1a
[Arabidopsis thaliana] GI:19574236; contains InterPro
entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM); contains Pfam profile PF00096:
Zinc finger, C2H2 type
Length = 613
Score = 28.7 bits (61), Expect = 3.7
Identities = 10/33 (30%), Positives = 21/33 (63%)
Frame = +3
Query: 516 KEICNRSIYAKGFAKDASLDDLLNYFKQFQEVE 614
++I R+I+ +GF D + ++L F+ + E+E
Sbjct: 403 RDIAQRNIFVRGFGWDTTQENLKTAFESYGEIE 435
>At1g52620.1 68414.m05941 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 819
Score = 28.3 bits (60), Expect = 4.9
Identities = 14/62 (22%), Positives = 29/62 (46%)
Frame = +3
Query: 423 IYEWIIGGF*R*QKSET*SRTATARNERGLRKEICNRSIYAKGFAKDASLDDLLNYFKQF 602
+Y +I GF R + + + E+G++ ++ + + KGF + LD+ L +
Sbjct: 487 VYATLIDGFIRSGDFDEARKVFSLSVEKGVKVDVVHHNAMIKGFCRSGMLDEALACMNRM 546
Query: 603 QE 608
E
Sbjct: 547 NE 548
>At1g22450.1 68414.m02806 cytochrome c oxidase subunit 6b, putative
(COX6b) nearly identical to subunit 6b of cytochrome c
oxidase [Arabidopsis thaliana] GI:6518353
Length = 191
Score = 28.3 bits (60), Expect = 4.9
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +3
Query: 153 KMTEDKEVTVETNGQEENAKTENSEDETELDIAI 254
K E KEV E Q E KTE +++E+ ++ A+
Sbjct: 29 KPVEVKEVAPEVTTQAEEVKTEQAKEESPVEEAV 62
>At4g11690.1 68417.m01867 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 566
Score = 27.9 bits (59), Expect = 6.5
Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -3
Query: 560 FSKSFRIN*SVAYF--FTKPSFISGSGCSGLRLTFLLSSETSN 438
+ +S +N S++YF F+ GS C LTF++ S + N
Sbjct: 104 YVQSQSLNLSISYFNEMVDNGFVPGSNCFNYLLTFVVGSSSFN 146
>At1g53650.1 68414.m06105 RNA-binding protein, putative similar to
RNA-binding protein GB:AAA86641 GI:1174153 from
[Arabidopsis thaliana]
Length = 314
Score = 27.9 bits (59), Expect = 6.5
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = +3
Query: 516 KEICNRSIYAKGFAKDASLDDLLNYFK 596
+E+C+R+IY K+A+ DD+ +F+
Sbjct: 220 REMCSRTIYCTNVDKNATEDDVNTFFQ 246
>At5g40870.1 68418.m04963 uracil phosphoribosyltransferase, putative
/ UMP pyrophosphorylase, putative / UPRTase, putative
similar to SP|O65583 Uracil phosphoribosyltransferase
(EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase)
{Arabidopsis thaliana}; contains Pfam profile PF00485:
Phosphoribulokinase / Uridine kinase family
Length = 486
Score = 27.5 bits (58), Expect = 8.6
Identities = 17/39 (43%), Positives = 21/39 (53%)
Frame = -3
Query: 497 SGSGCSGLRLTFLLSSETSNNPLVDLLRAFAMTSVSSVS 381
SG SGLR LLSS N+ +V LR+ +S S S
Sbjct: 16 SGPHFSGLRFDGLLSSSPPNSSVVSSLRSAVSSSSPSSS 54
>At5g15430.1 68418.m01806 calmodulin-binding protein-related has
weak similarity to calmodulin-binding proteins
Length = 478
Score = 27.5 bits (58), Expect = 8.6
Identities = 10/41 (24%), Positives = 25/41 (60%)
Frame = +3
Query: 147 KIKMTEDKEVTVETNGQEENAKTENSEDETELDIAIFGKSN 269
K + ++DK +ET ++E+++ E E+E + ++++ N
Sbjct: 294 KSEKSQDKGECIETEHEDESSQEEEDEEEEDENVSVSEDKN 334
>At5g01370.1 68418.m00050 expressed protein
Length = 427
Score = 27.5 bits (58), Expect = 8.6
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = +1
Query: 373 LAKLTEDTDVIANALNKSTSGLLEVSEDNKKVRRNPEQPLPEMNEGFVKKY 525
L KL E +++ ++ + +V ED+KK P P + NE KK+
Sbjct: 174 LDKLDEKRELVGSSSFSRSKRFEKVVEDSKKPPLPPSAPKKKENEKVAKKF 224
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,662,799
Number of Sequences: 28952
Number of extensions: 268960
Number of successful extensions: 1058
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 940
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1051
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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