Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20083
(676 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P48735 Cluster: Isocitrate dehydrogenase [NADP], mitoch... 136 5e-31
UniRef50_Q9SRZ6 Cluster: F12P19.10 protein; n=26; cellular organ... 136 6e-31
UniRef50_UPI0000F1EC8D Cluster: PREDICTED: similar to Isocitrate... 133 4e-30
UniRef50_Q8LPJ5 Cluster: Isocitrate dehydrogenase-like protein; ... 132 6e-30
UniRef50_A5N5L9 Cluster: Idh; n=2; Bacteria|Rep: Idh - Clostridi... 114 2e-24
UniRef50_A5ZVX5 Cluster: Putative uncharacterized protein; n=1; ... 113 4e-24
UniRef50_A2WMU2 Cluster: Putative uncharacterized protein; n=5; ... 111 1e-23
UniRef50_Q0CXI1 Cluster: Isocitrate dehydrogenase, mitochondrial... 99 5e-20
UniRef50_Q5DBI8 Cluster: SJCHGC09598 protein; n=1; Schistosoma j... 90 5e-17
UniRef50_A4VDP7 Cluster: Putative uncharacterized protein; n=1; ... 86 8e-16
UniRef50_Q2K7T8 Cluster: NADP-dependent isocitrate dehydrogenase... 57 3e-07
UniRef50_A3K670 Cluster: NADP-dependent isocitrate dehydrogenase... 52 2e-05
UniRef50_Q00WM7 Cluster: COG0538: Isocitrate dehydrogenases; n=3... 51 3e-05
UniRef50_A2XVE4 Cluster: Putative uncharacterized protein; n=2; ... 48 3e-04
UniRef50_A3JDN6 Cluster: Isocitrate dehydrogenase; n=5; Gammapro... 46 6e-04
UniRef50_Q40658 Cluster: Isocitrate dehydrogenase; n=1; Oryza sa... 46 0.001
UniRef50_Q67N12 Cluster: 3-isopropylmalate dehydrogenase; n=5; B... 40 0.042
>UniRef50_P48735 Cluster: Isocitrate dehydrogenase [NADP],
mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate
decarboxylase) (IDH) (NADP(+)-specific ICDH); n=493;
cellular organisms|Rep: Isocitrate dehydrogenase [NADP],
mitochondrial precursor (EC 1.1.1.42) (Oxalosuccinate
decarboxylase) (IDH) (NADP(+)-specific ICDH) - Homo
sapiens (Human)
Length = 452
Score = 136 bits (329), Expect = 5e-31
Identities = 62/84 (73%), Positives = 70/84 (83%)
Frame = +1
Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435
DGDEMTRI+W IKE+LI P+V + YFDLGLP+RD TDDQVTIDSA A K++V +KC
Sbjct: 54 DGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKC 113
Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507
ATITPDE RVEEFKLKKMW SPNG
Sbjct: 114 ATITPDEARVEEFKLKKMWKSPNG 137
Score = 101 bits (243), Expect = 1e-20
Identities = 43/56 (76%), Positives = 48/56 (85%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPG 665
P IRNILGGTVFREPI+C++IPR+VPGWTKPI IGRHAHGDQYKA DFV + G
Sbjct: 135 PNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAG 190
>UniRef50_Q9SRZ6 Cluster: F12P19.10 protein; n=26; cellular
organisms|Rep: F12P19.10 protein - Arabidopsis thaliana
(Mouse-ear cress)
Length = 410
Score = 136 bits (328), Expect = 6e-31
Identities = 60/84 (71%), Positives = 71/84 (84%)
Frame = +1
Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435
DGDEMTR++W IK++LI P+V++D YFDLGLPHRDATDD+VTI+SA A K+NV IKC
Sbjct: 16 DGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAEATKKYNVAIKC 75
Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507
ATITPDE RV EF LK+MW SPNG
Sbjct: 76 ATITPDEGRVTEFGLKQMWRSPNG 99
Score = 94.7 bits (225), Expect = 2e-18
Identities = 39/58 (67%), Positives = 48/58 (82%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKV 671
P IRNIL GTVFREPI+C+++P++VPGWTKPI IGRHA GDQY+A D V+ PGK+
Sbjct: 97 PNGTIRNILNGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKL 154
>UniRef50_UPI0000F1EC8D Cluster: PREDICTED: similar to Isocitrate
dehydrogenase 1 (NADP+), soluble; n=2; Danio rerio|Rep:
PREDICTED: similar to Isocitrate dehydrogenase 1
(NADP+), soluble - Danio rerio
Length = 206
Score = 133 bits (321), Expect = 4e-30
Identities = 56/83 (67%), Positives = 74/83 (89%)
Frame = +1
Query: 259 GDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKCA 438
GDEMTR++W IKE+LIFPY+++D +DLG+ +RDATDD+VT+++A A+ ++NVGIKCA
Sbjct: 22 GDEMTRVIWELIKEKLIFPYLELDLHSYDLGMENRDATDDKVTVEAAEAVRRYNVGIKCA 81
Query: 439 TITPDEQRVEEFKLKKMWLSPNG 507
TITPDE+RVEEFKLK+MW SPNG
Sbjct: 82 TITPDEKRVEEFKLKQMWRSPNG 104
Score = 82.6 bits (195), Expect = 8e-15
Identities = 34/44 (77%), Positives = 39/44 (88%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQ 629
P IRNILGGTVFRE I+C++IPR+VPGW KPI+IGRHAHGDQ
Sbjct: 102 PNGTIRNILGGTVFREAIICKNIPRLVPGWIKPIIIGRHAHGDQ 145
>UniRef50_Q8LPJ5 Cluster: Isocitrate dehydrogenase-like protein;
n=6; core eudicotyledons|Rep: Isocitrate
dehydrogenase-like protein - Arabidopsis thaliana
(Mouse-ear cress)
Length = 485
Score = 132 bits (320), Expect = 6e-30
Identities = 58/84 (69%), Positives = 71/84 (84%)
Frame = +1
Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435
DGDEMTR++W+ IKE+LI PY+ +D YFDLG+ +RDATDD+VT++SA A LK+NV IKC
Sbjct: 86 DGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYNVAIKC 145
Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507
ATITPDE RV+EF LK MW SPNG
Sbjct: 146 ATITPDEGRVKEFGLKSMWRSPNG 169
Score = 93.5 bits (222), Expect = 4e-18
Identities = 40/59 (67%), Positives = 47/59 (79%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674
P IRNIL GTVFREPI+C +IPR+VPGW KPI IGRHA GDQY+A D V+ PGK++
Sbjct: 167 PNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPICIGRHAFGDQYRATDTVIKGPGKLK 225
>UniRef50_A5N5L9 Cluster: Idh; n=2; Bacteria|Rep: Idh - Clostridium
kluyveri DSM 555
Length = 401
Score = 114 bits (275), Expect = 2e-24
Identities = 49/84 (58%), Positives = 67/84 (79%)
Frame = +1
Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435
DGDEMTRI+W IKE L+ PY+ + Y+DLGL R+ T+D++TI++A+AI K+ VG+KC
Sbjct: 15 DGDEMTRIIWKMIKELLLEPYIDLKTEYYDLGLVKRNETNDEITIEAANAIKKYGVGVKC 74
Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507
ATITP+ +RV+E+ LK MW SPNG
Sbjct: 75 ATITPNAKRVKEYNLKSMWKSPNG 98
Score = 64.9 bits (151), Expect = 2e-09
Identities = 29/59 (49%), Positives = 39/59 (66%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674
P IR IL GTVFR PI+ SI ++ W KPI + RHA+GD Y+ ++ V +PGK+E
Sbjct: 96 PNGTIRAILDGTVFRTPIIVNSIRPLMRTWEKPITVARHAYGDVYRDVEYKVEEPGKME 154
>UniRef50_A5ZVX5 Cluster: Putative uncharacterized protein; n=1;
Ruminococcus obeum ATCC 29174|Rep: Putative
uncharacterized protein - Ruminococcus obeum ATCC 29174
Length = 417
Score = 113 bits (272), Expect = 4e-24
Identities = 48/84 (57%), Positives = 66/84 (78%)
Frame = +1
Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435
DGDEMTRI+W IK+ L+ P++ ++ Y+DLGL +R+ TDDQVTID+A A K+ V +KC
Sbjct: 29 DGDEMTRILWKMIKDELLLPFIDLNTEYYDLGLNYRNETDDQVTIDAAEATKKYGVAVKC 88
Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507
ATITP+ R++E+ LKKM+ SPNG
Sbjct: 89 ATITPNHARMDEYDLKKMYKSPNG 112
Score = 67.3 bits (157), Expect = 3e-10
Identities = 31/59 (52%), Positives = 37/59 (62%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674
P IR IL GTVFR PI+ + I V W KPI + RHA+GD YK + + KPGKVE
Sbjct: 110 PNGTIRAILDGTVFRAPIVVKGIEPCVRNWKKPITLARHAYGDIYKNTEMYIDKPGKVE 168
>UniRef50_A2WMU2 Cluster: Putative uncharacterized protein; n=5;
Eukaryota|Rep: Putative uncharacterized protein - Oryza
sativa subsp. indica (Rice)
Length = 475
Score = 111 bits (268), Expect = 1e-23
Identities = 57/104 (54%), Positives = 70/104 (67%), Gaps = 18/104 (17%)
Frame = +1
Query: 250 REDGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGI 429
R GDEMTR++W IK++LIFP++ +D Y+DLGLP+RDAT D+VTI+SA A LK+NV I
Sbjct: 39 RNSGDEMTRVIWKWIKDKLIFPFLDLDIKYYDLGLPNRDATGDKVTIESAEATLKYNVAI 98
Query: 430 KCATITP------------------DEQRVEEFKLKKMWLSPNG 507
KCATITP DE RV+EF L MW SPNG
Sbjct: 99 KCATITPVLDTQFKFDFGRTIHEPTDEGRVKEFNLSAMWKSPNG 142
Score = 94.3 bits (224), Expect = 2e-18
Identities = 40/59 (67%), Positives = 48/59 (81%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674
P IRNIL GTVFREPI+C++IPR+VPGW KPI IGRHA GDQY+A D V+ PGK++
Sbjct: 140 PNGTIRNILNGTVFREPIICKNIPRLVPGWIKPICIGRHAFGDQYRATDTVIKGPGKLK 198
>UniRef50_Q0CXI1 Cluster: Isocitrate dehydrogenase, mitochondrial;
n=2; Eurotiomycetidae|Rep: Isocitrate dehydrogenase,
mitochondrial - Aspergillus terreus (strain NIH 2624)
Length = 466
Score = 99 bits (238), Expect = 5e-20
Identities = 43/59 (72%), Positives = 50/59 (84%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674
P IRNILGGTVFREPI+ +IPR+VPGWTKPI+IGRHA GDQY+A D V+P PGK+E
Sbjct: 151 PNGTIRNILGGTVFREPIVIPAIPRLVPGWTKPIIIGRHAFGDQYRATDRVIPGPGKLE 209
Score = 91.9 bits (218), Expect = 1e-17
Identities = 39/53 (73%), Positives = 47/53 (88%)
Frame = +1
Query: 349 GLPHRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWLSPNG 507
G+ +RD TDD+VT+++A AI K+ VG+KCATITPDE RVEEFKLKKMWLSPNG
Sbjct: 101 GIEYRDQTDDKVTVEAAEAIKKYGVGVKCATITPDEARVEEFKLKKMWLSPNG 153
>UniRef50_Q5DBI8 Cluster: SJCHGC09598 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC09598 protein - Schistosoma
japonicum (Blood fluke)
Length = 129
Score = 89.8 bits (213), Expect = 5e-17
Identities = 48/73 (65%), Positives = 53/73 (72%)
Frame = -3
Query: 509 LPFGLSHIFFNLNSSTLCSSGVIVAHLMPTLCFRMACAESMVT*SSVASRCGRPKSK*RQ 330
+PFG SHIFF LNS T CSSGV+VAHL+PTL F +A A S+VT S V SR GRPKSK Q
Sbjct: 15 VPFGDSHIFFKLNSLTRCSSGVMVAHLIPTLYFLIASAPSIVTWSLVRSRLGRPKSKYLQ 74
Query: 329 STLTYGNISLSLI 291
ST GNIS I
Sbjct: 75 STSINGNISCCFI 87
>UniRef50_A4VDP7 Cluster: Putative uncharacterized protein; n=1;
Tetrahymena thermophila SB210|Rep: Putative
uncharacterized protein - Tetrahymena thermophila SB210
Length = 141
Score = 85.8 bits (203), Expect = 8e-16
Identities = 49/85 (57%), Positives = 58/85 (68%)
Frame = -3
Query: 509 LPFGLSHIFFNLNSSTLCSSGVIVAHLMPTLCFRMACAESMVT*SSVASRCGRPKSK*RQ 330
+P G HIF +LNS T SS VIVAHL+PTLC R+A A S VT S VASR +S+
Sbjct: 5 VPLGEGHIFLSLNSLTRASSAVIVAHLIPTLCSRIAQAASKVTQSLVASRFSIERSQYLI 64
Query: 329 STLTYGNISLSLILAHMMRVISSPS 255
S GN+SLSLI+ M+RVISSPS
Sbjct: 65 SAEINGNMSLSLIIFQMIRVISSPS 89
>UniRef50_Q2K7T8 Cluster: NADP-dependent isocitrate dehydrogenase
protein; n=1; Rhizobium etli CFN 42|Rep: NADP-dependent
isocitrate dehydrogenase protein - Rhizobium etli
(strain CFN 42 / ATCC 51251)
Length = 437
Score = 57.2 bits (132), Expect = 3e-07
Identities = 30/80 (37%), Positives = 45/80 (56%)
Frame = -2
Query: 507 SVRA*PHFLQLELFYSLFIRCDSSAFDAHIML*NGMR*VDGDLIISGVTMREAQVKVKTI 328
+VRA PHFL++E + FIR D A D + +G+ VD L++ GV + + +V + I
Sbjct: 326 AVRALPHFLEIEFLDAGFIRRDRCALDTDAVFLDGVGGVDRHLVVGGVAIFDREVVIVDI 385
Query: 327 HLNIWKYQSLFDLSPHDACH 268
+ I Q + D P DACH
Sbjct: 386 EVEIRMDQLILDELPDDACH 405
>UniRef50_A3K670 Cluster: NADP-dependent isocitrate dehydrogenase
protein; n=2; Rhodobacteraceae|Rep: NADP-dependent
isocitrate dehydrogenase protein - Sagittula stellata
E-37
Length = 459
Score = 51.6 bits (118), Expect = 2e-05
Identities = 27/75 (36%), Positives = 39/75 (52%)
Frame = -2
Query: 492 PHFLQLELFYSLFIRCDSSAFDAHIML*NGMR*VDGDLIISGVTMREAQVKVKTIHLNIW 313
PH L+ E +L +R D AFD L VDGDL+ V + + ++ VK + + +
Sbjct: 362 PHLLETEFLDTLLVRGDRRAFDRDANLLRLFGGVDGDLVPGPVPLLDPEIVVKQVQVEVR 421
Query: 312 KYQSLFDLSPHDACH 268
+ Q D SPHDA H
Sbjct: 422 QDQLFLDESPHDAGH 436
Score = 34.7 bits (76), Expect = 2.1
Identities = 22/55 (40%), Positives = 28/55 (50%), Gaps = 1/55 (1%)
Frame = -3
Query: 647 KILRLVLVAVRMTAYHNRFRPTRHNPRDTLTQYRFAEYCTAQNVTDLPFG-LSHI 486
+I LVLVA + A+H+ P RH R R AE AQ+V D G L H+
Sbjct: 310 EIRGLVLVAEGVPAHHDGRGPARHEARHVAADDRLAEDDAAQDVADRAVGRLPHL 364
>UniRef50_Q00WM7 Cluster: COG0538: Isocitrate dehydrogenases; n=3;
Ostreococcus|Rep: COG0538: Isocitrate dehydrogenases -
Ostreococcus tauri
Length = 429
Score = 50.8 bits (116), Expect = 3e-05
Identities = 33/90 (36%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
Frame = +1
Query: 259 GDEMTRIMWAKIKERLIFPYVKVDCLY-FDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435
G+EMT + I+ R I P V V FDL +RD T+D+V D A + K
Sbjct: 36 GEEMTAYVMDLIRSRWIEPRVDVGGWETFDLRAKNRDDTEDRVLRDVIEAGKRIKAIFKE 95
Query: 436 ATITPDEQRVEEFKLKKMWLSPNGRSVTFW 525
T+TP +V+ L+K W SPNG W
Sbjct: 96 PTVTPTADQVKRLGLRKSWGSPNGAMRRGW 125
>UniRef50_A2XVE4 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 371
Score = 47.6 bits (108), Expect = 3e-04
Identities = 21/36 (58%), Positives = 27/36 (75%), Gaps = 2/36 (5%)
Frame = +3
Query: 498 PERKIRNILGGTVFREPILCQSIPRV--VPGWTKPI 599
P IRNIL GTVFREPILC+++PR+ VP +P+
Sbjct: 5 PNGTIRNILNGTVFREPILCKNVPRILSVPDGAEPV 40
>UniRef50_A3JDN6 Cluster: Isocitrate dehydrogenase; n=5;
Gammaproteobacteria|Rep: Isocitrate dehydrogenase -
Marinobacter sp. ELB17
Length = 582
Score = 46.4 bits (105), Expect = 6e-04
Identities = 22/80 (27%), Positives = 45/80 (56%)
Frame = +1
Query: 259 GDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKCA 438
GDEM ++ + +I ++ + + ++ + DL HR T+ QV ++ A+ K+ VG+K A
Sbjct: 16 GDEMAQVAFEEILKKFVTTRLAIELIEIDLSAEHRFLTNGQVIFEAIEALKKYGVGVKNA 75
Query: 439 TITPDEQRVEEFKLKKMWLS 498
+T + ++++ K LS
Sbjct: 76 GMTVNREQLDAMLEKHPELS 95
>UniRef50_Q40658 Cluster: Isocitrate dehydrogenase; n=1; Oryza
sativa|Rep: Isocitrate dehydrogenase - Oryza sativa
(Rice)
Length = 61
Score = 45.6 bits (103), Expect = 0.001
Identities = 17/27 (62%), Positives = 23/27 (85%)
Frame = +1
Query: 250 REDGDEMTRIMWAKIKERLIFPYVKVD 330
R+DGDEMTRI W IK++LIFP++ +D
Sbjct: 33 RDDGDEMTRIFWQSIKDKLIFPFLDLD 59
>UniRef50_Q67N12 Cluster: 3-isopropylmalate dehydrogenase; n=5;
Bacteria|Rep: 3-isopropylmalate dehydrogenase -
Symbiobacterium thermophilum
Length = 357
Score = 40.3 bits (90), Expect = 0.042
Identities = 17/38 (44%), Positives = 27/38 (71%)
Frame = +1
Query: 340 FDLGLPHRDATDDQVTIDSAHAILKHNVGIKCATITPD 453
+DL L +R AT ++V ++A A+ +H G+K ATITP+
Sbjct: 41 YDLSLENRRATSNRVVYEAAAAMREHGYGLKAATITPE 78
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 665,507,013
Number of Sequences: 1657284
Number of extensions: 13007695
Number of successful extensions: 30393
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 29634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30392
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 52066120554
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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