BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20083 (676 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 126 3e-30 SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 27 1.9 SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyc... 27 1.9 SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 25 7.6 SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 25 7.6 >SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 418 Score = 126 bits (304), Expect = 3e-30 Identities = 53/84 (63%), Positives = 70/84 (83%) Frame = +1 Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435 DGDEMTR++W I+E+L+ PY+ + Y+DLG+ RD T+DQ+T+D+A AILK++VGIKC Sbjct: 24 DGDEMTRVIWKIIREKLVLPYMDIKLDYYDLGIEARDKTNDQITVDAAKAILKNDVGIKC 83 Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507 ATITPDE RV+E+ LKKMW SPNG Sbjct: 84 ATITPDEARVKEYNLKKMWKSPNG 107 Score = 90.2 bits (214), Expect = 2e-19 Identities = 39/59 (66%), Positives = 45/59 (76%) Frame = +3 Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674 P IRNIL GTVFREPIL ++IP+ +PGWT PI IGRHA GDQYK+ D V PGK+E Sbjct: 105 PNGTIRNILNGTVFREPILIKNIPKYIPGWTNPICIGRHAFGDQYKSTDLVASGPGKLE 163 >SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 242 Score = 27.5 bits (58), Expect = 1.9 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +1 Query: 373 DDQVTIDSAHAILKHNVGIKCATITPDEQRVEEFKLKKMWL 495 DD VT + AIL+H V + P E+ V E + ++W+ Sbjct: 56 DDGVTYIESAAILEHLVRKYGPSFKPSEEDVAELEKYELWM 96 >SPAC1F5.07c |hem14||protoporphyrinogen oxidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 490 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/25 (52%), Positives = 17/25 (68%) Frame = -3 Query: 317 YGNISLSLILAHMMRVISSPSSRQL 243 YGNI+L LI A + R I SP+ + L Sbjct: 201 YGNITLGLIRALLAREILSPAEKAL 225 >SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1075 Score = 25.4 bits (53), Expect = 7.6 Identities = 23/79 (29%), Positives = 34/79 (43%), Gaps = 8/79 (10%) Frame = +1 Query: 346 LGLPHRDATDDQV----TIDSAHAILKHNVGIKCAT----ITPDEQRVEEFKLKKMWLSP 501 LGLP + + T + A + K + C T I+ E++ EE+K K L P Sbjct: 441 LGLPSKSIYKKTIKLVYTSEHAEEMFKALERMPCLTLPLVISRLEEKNEEWKSVKRSLQP 500 Query: 502 NGRSVTFWAVQYSANRYCV 558 RS+ F S + CV Sbjct: 501 GWRSIEFKNYDKSLDSQCV 519 >SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces pombe|chr 1|||Manual Length = 949 Score = 25.4 bits (53), Expect = 7.6 Identities = 17/78 (21%), Positives = 36/78 (46%) Frame = +1 Query: 292 IKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKCATITPDEQRVEE 471 ++ER+ ++ L +D+ PH D ++ + +L C + PD R+ E Sbjct: 833 MRERVAISCSQIASLSWDIDSPHGYIIDLSKSLIKLYKVLHRYSQRDCVEVIPDVIRMFE 892 Query: 472 FKLKKMWLSPNGRSVTFW 525 +L ++ L+ R+ + W Sbjct: 893 DRL-QLELTDIARNPSKW 909 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,743,586 Number of Sequences: 5004 Number of extensions: 54455 Number of successful extensions: 137 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 309878492 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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