BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20083 (676 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 136 2e-32 At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 135 3e-32 At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 132 2e-31 At3g51310.1 68416.m05616 vacuolar protein sorting-associated pro... 28 6.6 At5g49680.1 68418.m06151 cell expansion protein, putative simila... 27 8.7 At2g23450.2 68415.m02800 protein kinase family protein contains ... 27 8.7 At2g23450.1 68415.m02799 protein kinase family protein contains ... 27 8.7 >At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to isocitrate dehydrogenase SP|Q40345 from [Medicago sativa] Length = 410 Score = 136 bits (328), Expect = 2e-32 Identities = 60/84 (71%), Positives = 71/84 (84%) Frame = +1 Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435 DGDEMTR++W IK++LI P+V++D YFDLGLPHRDATDD+VTI+SA A K+NV IKC Sbjct: 16 DGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAEATKKYNVAIKC 75 Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507 ATITPDE RV EF LK+MW SPNG Sbjct: 76 ATITPDEGRVTEFGLKQMWRSPNG 99 Score = 94.7 bits (225), Expect = 5e-20 Identities = 39/58 (67%), Positives = 48/58 (82%) Frame = +3 Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKV 671 P IRNIL GTVFREPI+C+++P++VPGWTKPI IGRHA GDQY+A D V+ PGK+ Sbjct: 97 PNGTIRNILNGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKL 154 >At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to NADP-isocitrate dehydrogenase GI:5764653 from [Citrus limon]; Nicotiana tabacum SP|P50218 Length = 416 Score = 135 bits (326), Expect = 3e-32 Identities = 59/84 (70%), Positives = 72/84 (85%) Frame = +1 Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435 DGDEMTR++W IK++LIFP++++D YFDLGLP+RD TDD+VTI++A A LK+NV IKC Sbjct: 16 DGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAEATLKYNVAIKC 75 Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507 ATITPDE RV EF LKKMW SPNG Sbjct: 76 ATITPDEARVREFGLKKMWRSPNG 99 Score = 98.3 bits (234), Expect = 4e-21 Identities = 41/59 (69%), Positives = 50/59 (84%) Frame = +3 Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674 P IRNIL GTVFREPI+C++IPR+VPGWTKPI IGRHA GDQY+A D +V +PGK++ Sbjct: 97 PNGTIRNILNGTVFREPIICRNIPRLVPGWTKPICIGRHAFGDQYRATDLIVNEPGKLK 155 >At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to isocitrate dehydrogenase (NADP+) [Nicotiana tabacum] GI:3021512; contains Pfam domain PF00180: dehydrogenase, isocitrate/isopropylmalate family Length = 485 Score = 132 bits (320), Expect = 2e-31 Identities = 58/84 (69%), Positives = 71/84 (84%) Frame = +1 Query: 256 DGDEMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNVGIKC 435 DGDEMTR++W+ IKE+LI PY+ +D YFDLG+ +RDATDD+VT++SA A LK+NV IKC Sbjct: 86 DGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYNVAIKC 145 Query: 436 ATITPDEQRVEEFKLKKMWLSPNG 507 ATITPDE RV+EF LK MW SPNG Sbjct: 146 ATITPDEGRVKEFGLKSMWRSPNG 169 Score = 93.5 bits (222), Expect = 1e-19 Identities = 40/59 (67%), Positives = 47/59 (79%) Frame = +3 Query: 498 PERKIRNILGGTVFREPILCQSIPRVVPGWTKPIVIGRHAHGDQYKAQDFVVPKPGKVE 674 P IRNIL GTVFREPI+C +IPR+VPGW KPI IGRHA GDQY+A D V+ PGK++ Sbjct: 167 PNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPICIGRHAFGDQYRATDTVIKGPGKLK 225 >At3g51310.1 68416.m05616 vacuolar protein sorting-associated protein 35 family protein / VPS35 family protein similar to vacuolar protein sorting 35 [Mus musculus] GI:11875394; contains Pfam profile PF03635: Vacuolar protein sorting-associated protein 35 Length = 783 Score = 27.9 bits (59), Expect = 6.6 Identities = 19/43 (44%), Positives = 25/43 (58%), Gaps = 1/43 (2%) Frame = -2 Query: 582 QAQPSGYSDTISVRGILYRPKCYG-SSVRA*PHFLQLELFYSL 457 Q QPS T+ + G++ R Y SSV A P+FLQ+E F L Sbjct: 281 QLQPSVDIKTV-LSGLMERLSNYAASSVEALPNFLQVEAFSKL 322 >At5g49680.1 68418.m06151 cell expansion protein, putative similar to SABRE [Arabidopsis thaliana] GI:719291 Length = 1378 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/61 (21%), Positives = 33/61 (54%), Gaps = 1/61 (1%) Frame = +1 Query: 265 EMTRIMWAKIKERLIFPYVKVDCLYFDLGLPHRDATDDQVTIDSAHAILKHNV-GIKCAT 441 ++ +++W+ + + F V++D + +D +RD Q T + + +L++ + KC T Sbjct: 904 QINKVIWSMVLDGKTFAEVEIDNMIYDFNRDYRDIGIAQFT--TRYVVLRNCLPNAKCDT 961 Query: 442 I 444 + Sbjct: 962 V 962 >At2g23450.2 68415.m02800 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 708 Score = 27.5 bits (58), Expect = 8.7 Identities = 16/58 (27%), Positives = 28/58 (48%) Frame = -3 Query: 671 NLSGFRYNKILRLVLVAVRMTAYHNRFRPTRHNPRDTLTQYRFAEYCTAQNVTDLPFG 498 +L + + I + +A R A+H+ RPT D L Q R + + + ++ D P G Sbjct: 583 DLDAWTLSSIHTVAELAFRCLAFHSDMRPTMTEVADELEQIRLSGWIPSMSL-DSPAG 639 >At2g23450.1 68415.m02799 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 708 Score = 27.5 bits (58), Expect = 8.7 Identities = 16/58 (27%), Positives = 28/58 (48%) Frame = -3 Query: 671 NLSGFRYNKILRLVLVAVRMTAYHNRFRPTRHNPRDTLTQYRFAEYCTAQNVTDLPFG 498 +L + + I + +A R A+H+ RPT D L Q R + + + ++ D P G Sbjct: 583 DLDAWTLSSIHTVAELAFRCLAFHSDMRPTMTEVADELEQIRLSGWIPSMSL-DSPAG 639 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,490,025 Number of Sequences: 28952 Number of extensions: 293014 Number of successful extensions: 638 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 623 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 638 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1432596384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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