BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20079 (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC23G7.05 |sui1|psu1|translation initiation factor eIF1|Schizo... 48 1e-06 SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos... 28 1.1 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 28 1.1 SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1... 26 6.0 SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharom... 26 6.0 SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||... 25 7.9 >SPBC23G7.05 |sui1|psu1|translation initiation factor eIF1|Schizosaccharomyces pombe|chr 2|||Manual Length = 109 Score = 48.0 bits (109), Expect = 1e-06 Identities = 25/55 (45%), Positives = 33/55 (60%), Gaps = 1/55 (1%) Frame = +2 Query: 143 SIQNLNTFDPFADAIKSSEDDVQD-GLVHVRIQQRNGRRR*LRCKAFPRNMT*RR 304 +IQN NT DPFAD + +DD Q +H+RIQQRNGR+ + PR +R Sbjct: 3 AIQNFNTVDPFAD---TGDDDAQPLNNIHIRIQQRNGRKTLTTVQGLPREFDQKR 54 Score = 47.2 bits (107), Expect = 2e-06 Identities = 19/43 (44%), Positives = 32/43 (74%) Frame = +3 Query: 339 GTVVEHPEYGEVLQLQGDQRENICQWLTKSGLVKPEQLKVHGF 467 GT+V+ + GEV+QLQGDQR + ++LT+ ++ + +K+HGF Sbjct: 67 GTIVKDDDLGEVIQLQGDQRIKVMEFLTQQLGLQKKNIKIHGF 109 Score = 44.0 bits (99), Expect = 2e-05 Identities = 19/27 (70%), Positives = 23/27 (85%) Frame = +1 Query: 256 TLTTVQGLSSEYDLKKIVRACKKEFAC 336 TLTTVQGL E+D K+I+RA KK+FAC Sbjct: 39 TLTTVQGLPREFDQKRILRALKKDFAC 65 >SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 790 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/32 (37%), Positives = 21/32 (65%) Frame = -2 Query: 202 VLRAFDSIGEWVECVEILDGHTIERRVSLFQR 107 +LR S+ W+ ++ +G T+E+RV LF+R Sbjct: 25 LLRDPYSLKSWLRYIKTHEGSTLEKRVLLFER 56 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 28.3 bits (60), Expect = 1.1 Identities = 16/39 (41%), Positives = 19/39 (48%) Frame = -2 Query: 382 SCSTSPYSGCSTTVPCTRTPSCMPARSSSGHIPRKGLAP 266 SCS PY S T RTPS + SS P+ L+P Sbjct: 205 SCSPEPYLPLSPTRSPARTPSPIRLYSSDALRPQSPLSP 243 >SPAC4F8.07c |hxk2||hexokinase 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 25.8 bits (54), Expect = 6.0 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 6/64 (9%) Frame = +3 Query: 258 ADYGARPFLGI*PEEDRAGMQEGVRVQGTVVEH-PEYGEVL-----QLQGDQRENICQWL 419 A A P + RAG + V V G++VEH P + ++L +L GD + I + Sbjct: 372 ARMSAVPIASLYLSTGRAGKKSDVGVDGSLVEHYPHFVDMLREALRELIGDNEKLISIGI 431 Query: 420 TKSG 431 K G Sbjct: 432 AKDG 435 >SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharomyces pombe|chr 3|||Manual Length = 1690 Score = 25.8 bits (54), Expect = 6.0 Identities = 21/87 (24%), Positives = 42/87 (48%) Frame = +3 Query: 309 AGMQEGVRVQGTVVEHPEYGEVLQLQGDQRENICQWLTKSGLVKPEQLKVHGF*AALLKH 488 A + G + GTVV+ +YG ++++ G +NI KS + L + ++ K Sbjct: 1231 ADIAVGSNLDGTVVKVGDYGVLIRIDG--TDNIVGLCHKSEIADAVVLNISKLYSSGDKV 1288 Query: 489 NMSTLNMYYERLRVVIINPIKNMYLES 569 L++ E+ R+ + +K+ Y +S Sbjct: 1289 RAHVLDVDSEKRRIAL--GLKSSYFDS 1313 >SPBC25D12.06 |||RNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 565 Score = 25.4 bits (53), Expect = 7.9 Identities = 10/26 (38%), Positives = 18/26 (69%) Frame = +3 Query: 492 MSTLNMYYERLRVVIINPIKNMYLES 569 +STL+MY+E L+ + NP+ ++S Sbjct: 126 LSTLSMYFEALKFLKHNPVAGKPVQS 151 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,801,385 Number of Sequences: 5004 Number of extensions: 56149 Number of successful extensions: 163 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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