BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20070
(639 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g49970.2 68418.m06187 pyridoxamine 5'-phosphate oxidase-relat... 93 1e-19
At5g49970.1 68418.m06188 pyridoxamine 5'-phosphate oxidase-relat... 93 1e-19
At3g26125.1 68416.m03258 cytochrome P450, putative 29 2.6
At1g50020.1 68414.m05613 expressed protein 29 2.6
At5g59590.1 68418.m07467 UDP-glucoronosyl/UDP-glucosyl transfera... 29 3.4
At1g13150.1 68414.m01525 cytochrome P450, putative strong simila... 29 3.4
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 28 4.5
At1g13140.1 68414.m01523 cytochrome P450 family protein similar ... 28 6.0
>At5g49970.2 68418.m06187 pyridoxamine 5'-phosphate oxidase-related
contains weak similarity to Pyridoxamine 5'-phosphate
oxidase (EC 1.4.3.5) (PNP/PMP oxidase) (PNPOx).
(Swiss-Prot:P28225) [Shigella flexneri]
Length = 466
Score = 93.1 bits (221), Expect = 1e-19
Identities = 43/67 (64%), Positives = 52/67 (77%)
Frame = +2
Query: 308 YLNQSEAAALDQDLFTEYKFSVDQLMELAGLSVASAIAKVFPPSTHSSALIVCGPGNNGG 487
YL Q EAA +D+ L FS+DQLMELAGLSVA++IA+V+ P +S L +CGPGNNGG
Sbjct: 75 YLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVYKPEEYSRVLAICGPGNNGG 134
Query: 488 DGLVAAR 508
DGLVAAR
Sbjct: 135 DGLVAAR 141
Score = 36.3 bits (80), Expect = 0.017
Identities = 18/40 (45%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = +1
Query: 520 FGYNVSVHYPKRTPKPLYENLLEQCIRFNVNI--IDKLPD 633
FGY + YPKRT KPLY L+ Q +V ++ LPD
Sbjct: 146 FGYKPFICYPKRTAKPLYTGLVTQLDSLSVPFVSVEDLPD 185
>At5g49970.1 68418.m06188 pyridoxamine 5'-phosphate oxidase-related
contains weak similarity to Pyridoxamine 5'-phosphate
oxidase (EC 1.4.3.5) (PNP/PMP oxidase) (PNPOx).
(Swiss-Prot:P28225) [Shigella flexneri]
Length = 530
Score = 93.1 bits (221), Expect = 1e-19
Identities = 43/67 (64%), Positives = 52/67 (77%)
Frame = +2
Query: 308 YLNQSEAAALDQDLFTEYKFSVDQLMELAGLSVASAIAKVFPPSTHSSALIVCGPGNNGG 487
YL Q EAA +D+ L FS+DQLMELAGLSVA++IA+V+ P +S L +CGPGNNGG
Sbjct: 75 YLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVYKPEEYSRVLAICGPGNNGG 134
Query: 488 DGLVAAR 508
DGLVAAR
Sbjct: 135 DGLVAAR 141
Score = 36.3 bits (80), Expect = 0.017
Identities = 18/40 (45%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = +1
Query: 520 FGYNVSVHYPKRTPKPLYENLLEQCIRFNVNI--IDKLPD 633
FGY + YPKRT KPLY L+ Q +V ++ LPD
Sbjct: 146 FGYKPFICYPKRTAKPLYTGLVTQLDSLSVPFVSVEDLPD 185
>At3g26125.1 68416.m03258 cytochrome P450, putative
Length = 541
Score = 29.1 bits (62), Expect = 2.6
Identities = 11/22 (50%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
Frame = +2
Query: 521 SDTMYRYIILNAHPNLCM-KTF 583
SD ++Y++ NA P LC+ KTF
Sbjct: 464 SDDQFKYVVFNAGPRLCLGKTF 485
>At1g50020.1 68414.m05613 expressed protein
Length = 209
Score = 29.1 bits (62), Expect = 2.6
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = -1
Query: 555 AFRIMYRYIVSEQLHILAATKPSPPLFPGPHTIRADE*VEG 433
AF +Y Y ++ +H + T SPP + P+ + DE + G
Sbjct: 163 AFSALYLYFGADFIHDGSRTPVSPPPYIDPYALLEDERISG 203
>At5g59590.1 68418.m07467 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 449
Score = 28.7 bits (61), Expect = 3.4
Identities = 9/25 (36%), Positives = 16/25 (64%)
Frame = +3
Query: 30 RIIHNNTYHTIKCIIYDTYLYR*HA 104
+++H + I C++YD Y+Y HA
Sbjct: 98 QLLHEQCNNDIACVVYDEYMYFSHA 122
>At1g13150.1 68414.m01525 cytochrome P450, putative strong
similarity to gi|3313615 F21J9.9 from Arabidopsis
thaliana and is a member of the PF|00067 Cytochrome P450
family
Length = 529
Score = 28.7 bits (61), Expect = 3.4
Identities = 11/22 (50%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
Frame = +2
Query: 521 SDTMYRYIILNAHPNLCM-KTF 583
SD ++Y++ NA P LC+ KTF
Sbjct: 449 SDDQFKYVVFNAGPRLCIGKTF 470
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3)
{Arabidopsis thaliana}
Length = 1473
Score = 28.3 bits (60), Expect = 4.5
Identities = 19/67 (28%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Frame = -3
Query: 313 EVPRHSVALIHTARHSSDVISFATRN-IRALPNNLYNNI*EDSFTVFLLLIIDT*FGSKC 137
++ H VA ++ +++V + +N + N L +N DS T L + + FGSKC
Sbjct: 335 QITNHIVAAVNKKNKNANVKAHNVKNHLWVFVNALIDNPAFDSQTKETLTLRQSSFGSKC 394
Query: 136 QLK*YFI 116
+L F+
Sbjct: 395 ELSEDFL 401
>At1g13140.1 68414.m01523 cytochrome P450 family protein similar to
Cytochrome P450 86A2 (SP:O23066) [Arabidopsis thaliana];
contains Pfam PF|00067 Cytochrome P450 family
Length = 519
Score = 27.9 bits (59), Expect = 6.0
Identities = 10/22 (45%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
Frame = +2
Query: 521 SDTMYRYIILNAHPNLCM-KTF 583
+D ++Y++ NA P LC+ KTF
Sbjct: 441 NDDQFKYVVFNAGPRLCLGKTF 462
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,143,382
Number of Sequences: 28952
Number of extensions: 264799
Number of successful extensions: 588
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 588
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1314848736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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