Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20069
(636 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 137 1e-33
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 128 9e-31
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 126 2e-30
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 113 2e-26
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 97 1e-21
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 62 5e-11
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 56 6e-09
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 27 3.0
SPCC550.08 |||N-acetyltransferase |Schizosaccharomyces pombe|chr... 26 5.2
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 6.9
SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ... 25 6.9
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 25 6.9
SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyce... 25 9.1
SPAC22E12.18 |||conserved fungal protein|Schizosaccharomyces pom... 25 9.1
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 137 bits (331), Expect = 1e-33
Identities = 65/96 (67%), Positives = 77/96 (80%)
Frame = +2
Query: 251 EEKDKDIGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAA 430
+ + GTVIGIDLGTTYSCV V KNGRVEIIANDQGNRITPSYVAFT+D ERL+G+AA
Sbjct: 28 DNSTESYGTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTED-ERLVGEAA 86
Query: 431 KNQLTTNPENTVFDAKRLIGREWSDQLYNMMLSSSP 538
KNQ +NPENT+FD KRLIGR++ ++ + S P
Sbjct: 87 KNQAPSNPENTIFDIKRLIGRKFDEKTMAKDIKSFP 122
Score = 45.2 bits (102), Expect = 8e-06
Identities = 20/43 (46%), Positives = 27/43 (62%)
Frame = +1
Query: 508 TVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
T+ D+K FPF +V ++P V+V K F PEEISAM+L
Sbjct: 113 TMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMIL 155
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 128 bits (308), Expect = 9e-31
Identities = 63/107 (58%), Positives = 76/107 (71%)
Frame = +2
Query: 281 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 460
IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N
Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63
Query: 461 TVFDAKRLIGREWSDQLYNMMLSSSPSRSLKRTANLMFKYKLHKATK 601
T+FDAKRLIGR ++D + P + +++ + + + TK
Sbjct: 64 TIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETK 110
Score = 55.6 bits (128), Expect = 6e-09
Identities = 25/44 (56%), Positives = 32/44 (72%)
Frame = +1
Query: 505 PTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
P VQ D+K +PFKV+EK+ KP +QV+ K F PEEIS+MVL
Sbjct: 79 PEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVL 122
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 126 bits (305), Expect = 2e-30
Identities = 63/107 (58%), Positives = 75/107 (70%)
Frame = +2
Query: 281 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 460
IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP N
Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPHN 63
Query: 461 TVFDAKRLIGREWSDQLYNMMLSSSPSRSLKRTANLMFKYKLHKATK 601
T+FDAKRLIGR++ D + P + + + + + + TK
Sbjct: 64 TIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETK 110
Score = 52.8 bits (121), Expect = 4e-08
Identities = 24/44 (54%), Positives = 31/44 (70%)
Frame = +1
Query: 505 PTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
P VQ D+K +PFKV+ K+ KP +QV+ K F PEEIS+MVL
Sbjct: 79 PEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVL 122
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 113 bits (272), Expect = 2e-26
Identities = 54/75 (72%), Positives = 60/75 (80%)
Frame = +2
Query: 281 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 460
IGIDLGTTYSCV V++ VEII NDQG R TPS+VAFT+ ERL+GDAAKNQ NP N
Sbjct: 9 IGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTET-ERLVGDAAKNQAAMNPRN 67
Query: 461 TVFDAKRLIGREWSD 505
TVFDAKRLIGR + D
Sbjct: 68 TVFDAKRLIGRRYED 82
Score = 44.4 bits (100), Expect = 1e-05
Identities = 21/46 (45%), Positives = 28/46 (60%)
Frame = +1
Query: 499 E*PTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
E P Q D+K +PFKV++ N P ++V K F +EISAMVL
Sbjct: 81 EDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVL 126
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 97.5 bits (232), Expect = 1e-21
Identities = 46/82 (56%), Positives = 58/82 (70%)
Frame = +2
Query: 260 DKDIGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQ 439
+K G VIGIDLGTT SC+ + + ++IAN +G R TPS VAFT+DGERL+G +AK Q
Sbjct: 46 EKVKGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQ 105
Query: 440 LTTNPENTVFDAKRLIGREWSD 505
NPENT F KRLIGR + +
Sbjct: 106 AVINPENTFFATKRLIGRRFKE 127
Score = 27.9 bits (59), Expect = 1.3
Identities = 15/44 (34%), Positives = 27/44 (61%)
Frame = +1
Query: 505 PTVQHDVKFFPFKVVEKNSKPHVQVQTSQGDKVFAPEEISAMVL 636
P VQ D+K P+K+VE +S ++ ++G K ++P +I +L
Sbjct: 128 PEVQRDIKEVPYKIVE-HSNGDAWLE-ARG-KTYSPSQIGGFIL 168
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 62.5 bits (145), Expect = 5e-11
Identities = 30/76 (39%), Positives = 47/76 (61%)
Frame = +2
Query: 278 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 457
V+GID G + + + V +N +++I N+ NR TPS V++ + R +G+AAK+ +N
Sbjct: 7 VVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSY-GERSRFLGEAAKSAEASNFR 65
Query: 458 NTVFDAKRLIGREWSD 505
NTV KRL GR + D
Sbjct: 66 NTVGSLKRLAGRTYDD 81
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 55.6 bits (128), Expect = 6e-09
Identities = 29/89 (32%), Positives = 53/89 (59%), Gaps = 1/89 (1%)
Frame = +2
Query: 275 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 454
TVIGI G S + ++G+ +++AN++GNR PS +++ D E G A+ QL N
Sbjct: 25 TVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQE-YHGVQARGQLVRNA 83
Query: 455 ENTVFDAKRLIGREWSD-QLYNMMLSSSP 538
+N+V + + L+G+ + L++ S++P
Sbjct: 84 DNSVTNFRDLLGKSHDELTLHHCHYSANP 112
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 26.6 bits (56), Expect = 3.0
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = +2
Query: 242 RRQEEKDKDIGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNR 370
RR E+++ V G LG TY+ V ++G VE+ D N+
Sbjct: 1266 RRNGERNR-FSVVNGCFLGNTYAFASVTQDGSVEVHRLDVNNQ 1307
>SPCC550.08 |||N-acetyltransferase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 247
Score = 25.8 bits (54), Expect = 5.2
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = -1
Query: 579 YLNMRFAVLFNDLEGEELNIMLYSWSLHSRPI 484
YL +FN + E L I L S LH+RPI
Sbjct: 146 YLRKLIEPIFNICDQEGLPIYLESSHLHNRPI 177
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.4 bits (53), Expect = 6.9
Identities = 14/57 (24%), Positives = 28/57 (49%), Gaps = 3/57 (5%)
Frame = -1
Query: 180 KIYRLKDSEINKNLNKIIFTATIRSL*NAFIKHLRIQTVHRYFSF---NCSRKTRDS 19
KI+ +DS++ KN+ + + T + S A ++ +I +F C + RD+
Sbjct: 545 KIFNPEDSDLEKNITEALLTGDVLSAVKACLEEKKISEALFLSTFGGKECRKCVRDA 601
>SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 622
Score = 25.4 bits (53), Expect = 6.9
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +2
Query: 404 GERLIGDAAKNQLTTNPENT 463
GE ++ D+ N TNP+NT
Sbjct: 6 GENIVSDSIVNMELTNPDNT 25
>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
Upf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 925
Score = 25.4 bits (53), Expect = 6.9
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = -1
Query: 396 VKATYEGVMRFPWS 355
+K TYEG +R PWS
Sbjct: 284 MKLTYEGELRAPWS 297
>SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 285
Score = 25.0 bits (52), Expect = 9.1
Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 2/55 (3%)
Frame = -2
Query: 509 VGHSIHDQSDAWHQRQCFQGW**AGFWQHHQLN--VHHLE*RPRMKV*CGFPGHW 351
+G S D+ DA+++ QGW ++ QLN + E R+ C PG W
Sbjct: 1 MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSW 55
>SPAC22E12.18 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 336
Score = 25.0 bits (52), Expect = 9.1
Identities = 14/50 (28%), Positives = 21/50 (42%)
Frame = +2
Query: 338 VEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPENTVFDAKRLI 487
+E AND ITP A G+ +N+L E +F + L+
Sbjct: 80 LEKYANDMATTITPLIAAVQVFNAEKYGEIIQNRLKMYAERVLFGIEALL 129
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,685,351
Number of Sequences: 5004
Number of extensions: 56491
Number of successful extensions: 152
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -