BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20065
(649 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 31 0.14
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 30 0.33
SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.76
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch... 28 1.0
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 27 1.8
SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po... 27 3.1
SPCC1259.08 |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.1
SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|ch... 25 7.1
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 25 9.4
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 25 9.4
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 9.4
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 25 9.4
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 9.4
SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1 |Schizosacc... 25 9.4
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 31.1 bits (67), Expect = 0.14
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -3
Query: 332 RCVERCDVCCHMTLTCTNCYALIC 261
R VER D CCHM C + +C
Sbjct: 373 RVVERIDGCCHMNCLCGTHFCFLC 396
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 29.9 bits (64), Expect = 0.33
Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = +3
Query: 75 TPRQKVNTLRNVER-TETLPLRRGTCRHRFCTI*SEDF 185
+P QKVN + +E T T PL + T ++RF T + F
Sbjct: 256 SPLQKVNKQQQIESPTATFPLSKNTWKNRFHTFHKQSF 293
>SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 28.7 bits (61), Expect = 0.76
Identities = 15/38 (39%), Positives = 15/38 (39%)
Frame = -2
Query: 273 CPNLCSAYLVCFLGWPFVHRTELGHVFFDESLRFRSYR 160
CP L AY VC GW EL E R R R
Sbjct: 300 CPPLILAYTVCIYGWAVPSIAELEKEAIHEECRQRKRR 337
>SPAP8A3.14c |||mitochondrial inner membrane protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 677
Score = 28.3 bits (60), Expect = 1.0
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = -3
Query: 524 LNHYKKYFHLLHSILNEGKIYKKAIIIHWKI 432
L + K FH+ S L + +I ++ ++ HWK+
Sbjct: 127 LRKFAKVFHIKSSSLRKKEIIERILLDHWKL 157
>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 27.5 bits (58), Expect = 1.8
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +2
Query: 194 NTWPSSVLCTNGHPRKQTRYALHRLGH 274
NTW S++ C + + R T+Y H LGH
Sbjct: 340 NTWISTIRCNDCNSRCDTKY--HFLGH 364
>SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 594
Score = 26.6 bits (56), Expect = 3.1
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -2
Query: 276 LCPNLCSAYLVCFLGWP 226
L P+L S Y +CFLG+P
Sbjct: 42 LIPHLNSLYSICFLGFP 58
>SPCC1259.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 394
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = -3
Query: 404 NYKSSFQSHTSNEV 363
NYKSSF +H S+EV
Sbjct: 360 NYKSSFSAHVSSEV 373
>SPAC2G11.05c |||BRO1 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 701
Score = 25.4 bits (53), Expect = 7.1
Identities = 17/64 (26%), Positives = 33/64 (51%)
Frame = -3
Query: 539 LLSFTLNHYKKYFHLLHSILNEGKIYKKAIIIHWKILQSIFFVQNNYKSSFQSHTSNEVK 360
+LSF+ KKY LL ++ NE +I ++ I + + +N Y++ + N++K
Sbjct: 622 ILSFSQETEKKYKFLLQTVRNETEIARELYNIICRAEERYSQYENRYEN---ENRLNKIK 678
Query: 359 IVDS 348
+ S
Sbjct: 679 LQHS 682
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 25.0 bits (52), Expect = 9.4
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 466 FTKRLSSSIGKFFNQFSLFKIITSLVF 386
F RL+ GK N+F+LF + LVF
Sbjct: 801 FLSRLNVESGKAENKFNLFHLNQQLVF 827
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/20 (65%), Positives = 16/20 (80%), Gaps = 1/20 (5%)
Frame = +3
Query: 105 NVERTETLPLRR-GTCRHRF 161
+VERT LP +R GTCRH+F
Sbjct: 1566 SVERT--LPNKRCGTCRHKF 1583
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.0 bits (52), Expect = 9.4
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -1
Query: 511 KNIFICFILF*TKAKFTKRLSSSIGKFFNQFSLFKIITSLVF 386
+N+F+CF++ T LSS I N+ + ++ S+ F
Sbjct: 419 RNVFLCFVVGSKSCGKTALLSSFINNNTNRLTPNTVVNSVEF 460
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 25.0 bits (52), Expect = 9.4
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -3
Query: 440 WKILQSIFFVQNNYKSSFQSHTSN 369
W IL+S F NYKS + SN
Sbjct: 556 WDILESSFESSKNYKSDHREWLSN 579
>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 3071
Score = 25.0 bits (52), Expect = 9.4
Identities = 17/53 (32%), Positives = 25/53 (47%)
Frame = -2
Query: 600 LFYIDFDLHLITLTCHIYEGSFIFHIESLQKIFSSASFYFKRRQNLQKGYHHP 442
+F D D H T+ C +Y + H+E +K F A FY +N K + P
Sbjct: 1049 IFLYDCDQHFSTV-C-LYSANM--HMEFREKFFLQARFYDLEVKNHMKSNNPP 1097
>SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 25.0 bits (52), Expect = 9.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +2
Query: 287 TSVSCDSKRHIVRHISPVLGVNPLF 361
T +SC +I +++P LGVN L+
Sbjct: 652 TPLSCTEMLNISSYVNPSLGVNMLY 676
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,606,451
Number of Sequences: 5004
Number of extensions: 55138
Number of successful extensions: 139
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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