BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20063 (681 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 139 2e-33 At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 138 3e-33 At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 138 3e-33 At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.71 At3g13225.1 68416.m01660 WW domain-containing protein contains P... 29 2.8 At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 2.8 At2g24050.1 68415.m02873 MIF4G domain-containing protein / MA3 d... 28 6.6 At2g38570.1 68415.m04738 expressed protein ; expression supporte... 27 8.7 >At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal protein S4, Arabidopsis thaliana, PIR:T48480 Length = 262 Score = 139 bits (336), Expect = 2e-33 Identities = 71/132 (53%), Positives = 83/132 (62%) Frame = +3 Query: 255 DGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATG 434 DGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G Sbjct: 73 DGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFG 132 Query: 435 PKNVPYLVTHDGRTIRYPDPLIKVKIPSS*TLQLRRLWTSSSLSPGTCV*SREAVTWGVW 614 K +PYL T+DGRTIRYPDPLIK L+ ++ G V G Sbjct: 133 QKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRV 192 Query: 615 APSCSRERHPGS 650 +RE+H GS Sbjct: 193 GVIKNREKHKGS 204 Score = 122 bits (295), Expect = 2e-28 Identities = 56/75 (74%), Positives = 64/75 (85%) Frame = +1 Query: 40 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 219 MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60 Query: 220 VLKIVKQRLIKVMGK 264 V+ I+ QR I+V GK Sbjct: 61 VISILMQRHIQVDGK 75 Score = 67.7 bits (158), Expect = 7e-12 Identities = 30/63 (47%), Positives = 43/63 (68%) Frame = +2 Query: 491 PTYQSQDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTIVFPRETSRLFDIVHIK 670 P + D+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I + F+ +HI+ Sbjct: 152 PLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQ 211 Query: 671 DST 679 DST Sbjct: 212 DST 214 >At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) Length = 262 Score = 138 bits (334), Expect = 3e-33 Identities = 71/132 (53%), Positives = 83/132 (62%) Frame = +3 Query: 255 DGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATG 434 DGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G Sbjct: 73 DGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFG 132 Query: 435 PKNVPYLVTHDGRTIRYPDPLIKVKIPSS*TLQLRRLWTSSSLSPGTCV*SREAVTWGVW 614 K +PYL T+DGRTIRYPDPLIK L+ ++ G V G Sbjct: 133 QKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRV 192 Query: 615 APSCSRERHPGS 650 +RE+H GS Sbjct: 193 GVIKNREKHKGS 204 Score = 122 bits (295), Expect = 2e-28 Identities = 56/75 (74%), Positives = 64/75 (85%) Frame = +1 Query: 40 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 219 MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60 Query: 220 VLKIVKQRLIKVMGK 264 V+ I+ QR I+V GK Sbjct: 61 VISILMQRHIQVDGK 75 Score = 67.3 bits (157), Expect = 9e-12 Identities = 30/63 (47%), Positives = 43/63 (68%) Frame = +2 Query: 491 PTYQSQDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTIVFPRETSRLFDIVHIK 670 P + D+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I + F+ +HI+ Sbjct: 152 PLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQ 211 Query: 671 DST 679 DST Sbjct: 212 DST 214 >At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains ribosomal protein S4 signature from residues 8 to 22 Length = 261 Score = 138 bits (334), Expect = 3e-33 Identities = 71/132 (53%), Positives = 83/132 (62%) Frame = +3 Query: 255 DGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATG 434 DGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G Sbjct: 73 DGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFG 132 Query: 435 PKNVPYLVTHDGRTIRYPDPLIKVKIPSS*TLQLRRLWTSSSLSPGTCV*SREAVTWGVW 614 K +PYL T+DGRTIRYPDPLIK L+ ++ G V G Sbjct: 133 QKGIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRV 192 Query: 615 APSCSRERHPGS 650 +RE+H GS Sbjct: 193 GVIKNREKHKGS 204 Score = 122 bits (295), Expect = 2e-28 Identities = 56/75 (74%), Positives = 64/75 (85%) Frame = +1 Query: 40 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 219 MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT E Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYRE 60 Query: 220 VLKIVKQRLIKVMGK 264 V+ I+ QR I+V GK Sbjct: 61 VISILMQRHIQVDGK 75 Score = 67.3 bits (157), Expect = 9e-12 Identities = 30/63 (47%), Positives = 43/63 (68%) Frame = +2 Query: 491 PTYQSQDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTIVFPRETSRLFDIVHIK 670 P + D+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I + F+ +HI+ Sbjct: 152 PLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQ 211 Query: 671 DST 679 DST Sbjct: 212 DST 214 >At4g10710.1 68417.m01751 transcriptional regulator-related similar to chromatin-specific transcription elongation factor FACT 140 kDa subunit (GI:5499741) [Homo sapiens] Length = 1074 Score = 31.1 bits (67), Expect = 0.71 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -2 Query: 164 KHSRNLWGPVDGLGAYTPPSLSNIHAL 84 KHS +LWG D L TPP+ ++ L Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70 >At3g13225.1 68416.m01660 WW domain-containing protein contains Pfam profile PF00397: WW domain Length = 863 Score = 29.1 bits (62), Expect = 2.8 Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 5/63 (7%) Frame = +3 Query: 135 NRSPQVARVLAPGDFPEESSEVCFDRK-----RSPENCETTPHKGDGKVRTDPTYPAGFM 299 N+SP +++A + ES + D + +S + E+ GDG + P +PAG Sbjct: 388 NKSPPKEKLVADANTESESEDSERDGELAQSGKSLHSDESADGSGDGSPKHSPNHPAGQS 447 Query: 300 DVV 308 D++ Sbjct: 448 DII 450 >At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, putative / steroid 5-alpha-reductase, putative identical to gi:1280611; contains a steroid 5-alpha reductase, C-terminal domain Length = 262 Score = 29.1 bits (62), Expect = 2.8 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = -2 Query: 254 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 138 TF R C + P A +FL+ GKH+R WGP Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45 >At2g24050.1 68415.m02873 MIF4G domain-containing protein / MA3 domain-containing protein similar to SP|Q03387 Eukaryotic initiation factor (iso)4F subunit P82-34 (eIF-(iso)4F P82-34) {Triticum aestivum}; contains Pfam profiles PF02854: MIF4G domain, PF02847: MA3 domain Length = 747 Score = 27.9 bits (59), Expect = 6.6 Identities = 10/28 (35%), Positives = 18/28 (64%) Frame = -1 Query: 657 MSKSRDVSRGNTMVPTRPKLRPPVIIHK 574 M+++R + RGN +P+ +PP I+K Sbjct: 484 MARTRSMPRGNRQTVQQPRFQPPPAINK 511 >At2g38570.1 68415.m04738 expressed protein ; expression supported by MPSS Length = 302 Score = 27.5 bits (58), Expect = 8.7 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +2 Query: 536 TKIMDFIKFESGNLCMITGGRNLGRVGT 619 T I+ +IK G + GGRN+G+V T Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVST 223 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,528,115 Number of Sequences: 28952 Number of extensions: 366118 Number of successful extensions: 1055 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 1007 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1055 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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