BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20062 (635 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein Vps1|Schizo... 118 7e-28 SPBC12C2.08 |dnm1||dynamin Dnm1|Schizosaccharomyces pombe|chr 2|... 115 7e-27 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 76 5e-15 SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo... 28 1.3 SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 27 2.3 SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pomb... 26 4.0 SPBC651.12c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 6.9 SPAC4G8.15c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 6.9 SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pom... 25 6.9 >SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein Vps1|Schizosaccharomyces pombe|chr 1|||Manual Length = 678 Score = 118 bits (284), Expect = 7e-28 Identities = 54/104 (51%), Positives = 78/104 (75%) Frame = +1 Query: 256 QQIKGMIFQFIRRESCLILAVTPANTDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEG 435 +QI+ M+ ++I + + +ILAV ANTDLANSD LKLA+EVDP+GLRTIGV+TK+DLMD+G Sbjct: 171 KQIREMVLKYISKNNAIILAVNAANTDLANSDGLKLAREVDPEGLRTIGVLTKVDLMDKG 230 Query: 436 TDARDVLENKLLPLRRGYIGVVNRCRKTLTVART*PLHLRLREN 567 TD D+L +++PLR GY+ V+NR +K + ++ + L N Sbjct: 231 TDVVDILAGRVIPLRLGYVPVINRGQKDIEGKKSIRIALEAERN 274 Score = 109 bits (261), Expect = 4e-25 Identities = 49/78 (62%), Positives = 61/78 (78%) Frame = +2 Query: 20 NGNTEYAEFLHCKGKKFTDFNEVRGEIEAETDRVTGSNKGISPVPINLRVYSPNVLNLTL 199 N ++E+ EFLH G+KF +F ++R EI ET+ TG N GIS VPI LR+YSP+VL LTL Sbjct: 92 NNSSEWGEFLHLPGQKFFEFEKIREEIVRETEEKTGKNVGISSVPIYLRIYSPHVLTLTL 151 Query: 200 IDLPGLTKVPIGDQPIDI 253 +DLPGLTKVP+GDQP DI Sbjct: 152 VDLPGLTKVPVGDQPRDI 169 Score = 44.8 bits (101), Expect = 1e-05 Identities = 21/36 (58%), Positives = 24/36 (66%) Frame = +3 Query: 510 QKDIDGRKDIAAALAAERKFFLSHPSYRHLADRLGT 617 QKDI+G+K I AL AER FF +HPSY A GT Sbjct: 256 QKDIEGKKSIRIALEAERNFFETHPSYGSKAQYCGT 291 >SPBC12C2.08 |dnm1||dynamin Dnm1|Schizosaccharomyces pombe|chr 2|||Manual Length = 781 Score = 115 bits (276), Expect = 7e-27 Identities = 52/74 (70%), Positives = 61/74 (82%) Frame = +2 Query: 32 EYAEFLHCKGKKFTDFNEVRGEIEAETDRVTGSNKGISPVPINLRVYSPNVLNLTLIDLP 211 +YAEFLH KFTD N+VR EIE ET RV G+NKGI+ +PINL++YS VLNLTLIDLP Sbjct: 113 DYAEFLHIPDTKFTDMNKVRAEIENETLRVAGANKGINKLPINLKIYSTRVLNLTLIDLP 172 Query: 212 GLTKVPIGDQPIDI 253 GLTK+P+GDQP DI Sbjct: 173 GLTKIPVGDQPTDI 186 Score = 105 bits (251), Expect = 7e-24 Identities = 45/88 (51%), Positives = 67/88 (76%) Frame = +1 Query: 259 QIKGMIFQFIRRESCLILAVTPANTDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGT 438 Q + +I ++I R + +ILAV+PAN D+ NS+ LKLA+ VDP+G RTIGV+TKLDLMD+GT Sbjct: 189 QTRSLIMEYISRPNSIILAVSPANFDIVNSEGLKLARSVDPKGKRTIGVLTKLDLMDQGT 248 Query: 439 DARDVLENKLLPLRRGYIGVVNRCRKTL 522 +A D+L ++ PL+ G++ VNR + + Sbjct: 249 NAMDILSGRVYPLKLGFVATVNRSQSDI 276 Score = 39.9 bits (89), Expect = 3e-04 Identities = 18/36 (50%), Positives = 22/36 (61%) Frame = +3 Query: 510 QKDIDGRKDIAAALAAERKFFLSHPSYRHLADRLGT 617 Q DI K + AL +ER FF HP+YR + DR GT Sbjct: 273 QSDIVSHKSMRDALQSERSFFEHHPAYRTIKDRCGT 308 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 75.8 bits (178), Expect = 5e-15 Identities = 39/72 (54%), Positives = 51/72 (70%) Frame = +1 Query: 292 RESCLILAVTPANTDLANSDALKLAKEVDPQGLRTIGVITKLDLMDEGTDARDVLENKLL 471 RE +ILAV A+ DLANS AL+ ++ VDP GLRTIGV+TK+DL+ + A +L N Sbjct: 399 REPNIILAVCAADVDLANSAALRASRRVDPLGLRTIGVVTKMDLVPP-SKAISILHNNNY 457 Query: 472 PLRRGYIGVVNR 507 PL GYIGV++R Sbjct: 458 PLHYGYIGVISR 469 Score = 54.0 bits (124), Expect = 2e-08 Identities = 26/73 (35%), Positives = 44/73 (60%) Frame = +2 Query: 35 YAEFLHCKGKKFTDFNEVRGEIEAETDRVTGSNKGISPVPINLRVYSPNVLNLTLIDLPG 214 Y EF + K TDF++++ I + + S++G+ PI L +Y+ ++ NL+LIDLPG Sbjct: 315 YGEFSGVQLGKITDFSKIQ-HILTDLNMAVPSSQGVDDNPIRLTIYASHIPNLSLIDLPG 373 Query: 215 LTKVPIGDQPIDI 253 ++ DQP D+ Sbjct: 374 YIQIHSEDQPADL 386 >SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1401 Score = 27.9 bits (59), Expect = 1.3 Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Frame = +2 Query: 101 EAETDRVTGSNKGISPVPI--NLRVYSPNVLNLTLIDLPGLTKVP 229 +AETD + S K S +P+ N+ + PN L + I+LP P Sbjct: 1349 KAETDSLAASYKEESALPVASNVGLRQPNELRIDSINLPPAIVTP 1393 >SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 332 Score = 27.1 bits (57), Expect = 2.3 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +1 Query: 301 CLILAVTPANTDLANSDALKLAKE 372 CL +TP+ T + NSD+L L K+ Sbjct: 203 CLHCPLTPSTTHIVNSDSLALMKK 226 >SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pombe|chr 2|||Manual Length = 247 Score = 26.2 bits (55), Expect = 4.0 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 1/57 (1%) Frame = -3 Query: 228 GTFVRPGK-SISVRLSTFGE*TRRLIGTGDIPLLEPVTRSVSASISPRTSLKSVNFF 61 G GK SI + FG+ ++ G +LEP+ + A I+ L +NFF Sbjct: 59 GDVTEVGKASIETAIKKFGKLDSVILNAG---VLEPIAKIADADINEWRKLFDINFF 112 >SPBC651.12c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 25.4 bits (53), Expect = 6.9 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +1 Query: 295 ESCLILAVTPANTDLANSDALKL 363 E C +L + +NTD+ NS +L L Sbjct: 232 EKCTVLLLKRSNTDVDNSSSLSL 254 >SPAC4G8.15c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 46 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/45 (28%), Positives = 24/45 (53%) Frame = -2 Query: 247 YRLITDWHFCQTRQINQRQVEHIRGINTQVNRYRRYTLVGTSNTI 113 Y + H Q N+R++ HI + T+ +RY + ++ + NTI Sbjct: 3 YEIFIMQHLLIKSQSNERKITHIFHMLTKFDRYSK-MILNSINTI 46 >SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pombe|chr 1|||Manual Length = 355 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/48 (27%), Positives = 23/48 (47%) Frame = -2 Query: 250 IYRLITDWHFCQTRQINQRQVEHIRGINTQVNRYRRYTLVGTSNTICL 107 ++R+ H+C T+ Q + + ++ +TLVG TICL Sbjct: 164 VHRICNLLHWCNTKTEEQTRAALQSWLPKELWFELNHTLVGFGQTICL 211 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,541,053 Number of Sequences: 5004 Number of extensions: 48982 Number of successful extensions: 137 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 283719918 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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