BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20060
(530 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09690.1 68414.m01088 60S ribosomal protein L21 (RPL21C) Simi... 98 3e-21
At1g09590.1 68414.m01076 60S ribosomal protein L21 (RPL21A) Simi... 98 3e-21
At1g57860.1 68414.m06565 60S ribosomal protein L21 similar to 60... 97 9e-21
At1g57660.1 68414.m06543 60S ribosomal protein L21 (RPL21E) simi... 97 9e-21
At1g62330.1 68414.m07033 expressed protein contains Pfam PF03138... 36 0.017
At1g20020.1 68414.m02507 ferredoxin--NADP(+) reductase, putative... 29 1.5
At4g24350.1 68417.m03494 phosphorylase family protein contains P... 29 2.0
At5g25320.1 68418.m03004 ACT domain-containing protein contains ... 28 3.4
At5g05900.1 68418.m00651 UDP-glucoronosyl/UDP-glucosyl transfera... 28 3.4
At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase fa... 28 3.4
At2g13150.1 68415.m01450 expressed protein contains a bZIP trans... 28 3.4
At5g41220.1 68418.m05009 glutathione S-transferase, putative sim... 27 6.0
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-... 27 6.0
At3g45880.1 68416.m04965 hypothetical protein 27 6.0
At5g41240.1 68418.m05011 glutathione S-transferase, putative sim... 27 7.9
At1g76990.3 68414.m08966 ACT domain containing protein low simil... 27 7.9
At1g76990.2 68414.m08965 ACT domain containing protein low simil... 27 7.9
At1g76990.1 68414.m08964 ACT domain containing protein low simil... 27 7.9
>At1g09690.1 68414.m01088 60S ribosomal protein L21 (RPL21C) Similar
to ribosomal protein L21 (gb|L38826). ESTs
gb|AA395597,gb|ATTS5197 come from this gene
Length = 164
Score = 98.3 bits (234), Expect = 3e-21
Identities = 47/106 (44%), Positives = 66/106 (62%), Gaps = 1/106 (0%)
Frame = +3
Query: 39 GLPXRHQGLVRSQFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVY 218
G+ R + L FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++
Sbjct: 6 GVRARTRDLFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIW 65
Query: 219 NVTAHALGVIVNSVFAEGLYRS-ASISVLSMSSTPSADKTSLRESK 353
NVT A+GV VN + R + V + + A++ LR+ K
Sbjct: 66 NVTKRAVGVEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKK 111
Score = 75.8 bits (178), Expect = 2e-14
Identities = 36/84 (42%), Positives = 54/84 (64%)
Frame = +2
Query: 245 DCQQRVRGRIIPKRINIRVEHVKHSKCRQDFLKRVKENERLLKEAKAAGKTVNLKRQPAP 424
+ +++ RII KRI++RVEHV+ S+C ++F R K+N+ L AKA G+T++ KRQP
Sbjct: 75 EVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKKNDELKAAAKANGETISTKRQPKG 134
Query: 425 PKAAHIVSGTEKPVLLAPIPYEFV 496
PK +V G + PIPY+ V
Sbjct: 135 PKPGFMVEGMTLET-VTPIPYDVV 157
>At1g09590.1 68414.m01076 60S ribosomal protein L21 (RPL21A) Similar
to L21 family of ribosomal protein; amino acid sequence
is identical to F21M12.8
Length = 164
Score = 98.3 bits (234), Expect = 3e-21
Identities = 47/106 (44%), Positives = 66/106 (62%), Gaps = 1/106 (0%)
Frame = +3
Query: 39 GLPXRHQGLVRSQFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVY 218
G+ R + L FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++
Sbjct: 6 GVRARTRDLFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIW 65
Query: 219 NVTAHALGVIVNSVFAEGLYRS-ASISVLSMSSTPSADKTSLRESK 353
NVT A+GV VN + R + V + + A++ LR+ K
Sbjct: 66 NVTKRAVGVEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKK 111
Score = 75.8 bits (178), Expect = 2e-14
Identities = 36/84 (42%), Positives = 54/84 (64%)
Frame = +2
Query: 245 DCQQRVRGRIIPKRINIRVEHVKHSKCRQDFLKRVKENERLLKEAKAAGKTVNLKRQPAP 424
+ +++ RII KRI++RVEHV+ S+C ++F R K+N+ L AKA G+T++ KRQP
Sbjct: 75 EVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKKNDELKAAAKANGETISTKRQPKG 134
Query: 425 PKAAHIVSGTEKPVLLAPIPYEFV 496
PK +V G + PIPY+ V
Sbjct: 135 PKPGFMVEGMTLET-VTPIPYDVV 157
>At1g57860.1 68414.m06565 60S ribosomal protein L21 similar to 60S
ribosomal protein L21 GI:3885884 from [Oryza sativa]
Length = 164
Score = 96.7 bits (230), Expect = 9e-21
Identities = 46/106 (43%), Positives = 66/106 (62%), Gaps = 1/106 (0%)
Frame = +3
Query: 39 GLPXRHQGLVRSQFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVY 218
G+ R + L FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++
Sbjct: 6 GVRARTRDLFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIW 65
Query: 219 NVTAHALGVIVNSVFAEGLYRS-ASISVLSMSSTPSADKTSLRESK 353
NVT A+GV VN + R + V + + A++ LR+ +
Sbjct: 66 NVTKRAVGVEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKQ 111
Score = 76.6 bits (180), Expect = 1e-14
Identities = 36/84 (42%), Positives = 55/84 (65%)
Frame = +2
Query: 245 DCQQRVRGRIIPKRINIRVEHVKHSKCRQDFLKRVKENERLLKEAKAAGKTVNLKRQPAP 424
+ +++ RII KRI++RVEHV+ S+C ++F R K+N+ L +AKA G+T++ KRQP
Sbjct: 75 EVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKQNDVLKADAKARGETISTKRQPKG 134
Query: 425 PKAAHIVSGTEKPVLLAPIPYEFV 496
PK +V G + PIPY+ V
Sbjct: 135 PKPGFMVEGMTLET-VTPIPYDVV 157
>At1g57660.1 68414.m06543 60S ribosomal protein L21 (RPL21E) similar
to 60S ribosomal protein L21 GB:Q43291 GI:2851508 from
[Arabidopsis thaliana]
Length = 164
Score = 96.7 bits (230), Expect = 9e-21
Identities = 46/106 (43%), Positives = 66/106 (62%), Gaps = 1/106 (0%)
Frame = +3
Query: 39 GLPXRHQGLVRSQFRTHGTIPLSTYMKVYKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVY 218
G+ R + L FR G IPLSTY++ +KVGD VD++ NGA+ KGMPHK YHG+TGR++
Sbjct: 6 GVRARTRDLFARPFRKKGYIPLSTYLRTFKVGDYVDVKVNGAIHKGMPHKFYHGRTGRIW 65
Query: 219 NVTAHALGVIVNSVFAEGLYRS-ASISVLSMSSTPSADKTSLRESK 353
NVT A+GV VN + R + V + + A++ LR+ +
Sbjct: 66 NVTKRAVGVEVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKQ 111
Score = 76.6 bits (180), Expect = 1e-14
Identities = 36/84 (42%), Positives = 55/84 (65%)
Frame = +2
Query: 245 DCQQRVRGRIIPKRINIRVEHVKHSKCRQDFLKRVKENERLLKEAKAAGKTVNLKRQPAP 424
+ +++ RII KRI++RVEHV+ S+C ++F R K+N+ L +AKA G+T++ KRQP
Sbjct: 75 EVNKQIGNRIIRKRIHVRVEHVQQSRCAEEFKLRKKQNDVLKADAKARGETISTKRQPKG 134
Query: 425 PKAAHIVSGTEKPVLLAPIPYEFV 496
PK +V G + PIPY+ V
Sbjct: 135 PKPGFMVEGMTLET-VTPIPYDVV 157
>At1g62330.1 68414.m07033 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown;
Length = 672
Score = 35.9 bits (79), Expect = 0.017
Identities = 22/65 (33%), Positives = 30/65 (46%)
Frame = +3
Query: 174 GMPHKVYHGKTGRVYNVTAHALGVIVNSVFAEGLYRSASISVLSMSSTPSADKTSLRESK 353
G P K Y+G GR+ AHAL N + + L+R L+ P AD+ S + S
Sbjct: 182 GKPKKTYNGTYGRLLAYAAHALAEGQNKLEPKELWREPKDQALAWK--PCADQRSWKPSD 239
Query: 354 RMRGY 368
GY
Sbjct: 240 GKNGY 244
>At1g20020.1 68414.m02507 ferredoxin--NADP(+) reductase, putative /
adrenodoxin reductase, putative strong similarity to
Ferredoxin--NADP reductase, chloroplast precursor (EC
1.18.1.2) (FNR) from {Pisum sativum} SP|P10933,
{Mesembryanthemum crystallinum} SP|P41343, {Spinacia
oleracea} SP|P00455, [Capsicum annuum] GI:6899972
Length = 369
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/45 (35%), Positives = 25/45 (55%)
Frame = +3
Query: 123 YKVGDIVDIRGNGAVQKGMPHKVYHGKTGRVYNVTAHALGVIVNS 257
Y+ G V + +G + G PHKV R+Y++ + ALG + NS
Sbjct: 125 YREGQSVGVIADGIDKNGKPHKV------RLYSIASSALGDLGNS 163
>At4g24350.1 68417.m03494 phosphorylase family protein contains Pfam
PF01048: Phosphorylase family
Length = 336
Score = 29.1 bits (62), Expect = 2.0
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -1
Query: 149 DVYNVSNFVHFHVRGERNSSM 87
DV+NV VHF + G N+SM
Sbjct: 114 DVFNVKGIVHFGIAGNMNNSM 134
>At5g25320.1 68418.m03004 ACT domain-containing protein contains
Pfam ACT domain PF01842
Length = 500
Score = 28.3 bits (60), Expect = 3.4
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +2
Query: 161 CSSKGYATQSIPWKDRSRVQRDCSCSRCDCQ 253
C +GY+ ++ KDR R+ D C+ D Q
Sbjct: 291 CEERGYSIVTVKSKDRRRLMFDTICTLVDMQ 321
>At5g05900.1 68418.m00651 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 450
Score = 28.3 bits (60), Expect = 3.4
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = -1
Query: 188 FVWHTLLNCTIASDVYNVSNFVHFHVRGERN 96
FVW LLN SDV+ V +H R ERN
Sbjct: 369 FVWDQLLNARFVSDVWMVG--LHLEGRIERN 397
>At2g43900.1 68415.m05457 endonuclease/exonuclease/phosphatase family
protein belongs to Pfam:PF03372:
Endonuclease/Exonuclease/phosphatase family; contains 3
WD-40 repeats (PF00400);similar to Type II
inositol-1,4,5-trisphosphate 5-phosphatase EC 3.1.3.56
(5PTASE) (INPP5B) (SP:P32019) [Homo sapiens]
Length = 1305
Score = 28.3 bits (60), Expect = 3.4
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = +2
Query: 284 RINIRVEHVKHSKCRQDFLKRVKENERL 367
+ ++++EHV S RQ+F + +K NE++
Sbjct: 920 KFHVKIEHVDRSVRRQEFGRIIKTNEKV 947
>At2g13150.1 68415.m01450 expressed protein contains a bZIP
transcription factor basic domain signature (PDOC00036)
Length = 262
Score = 28.3 bits (60), Expect = 3.4
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = +3
Query: 303 SMSSTPSADKTSLRESKRMRGY*RKPRLPARPST*RDSQLPLKLPTSSVELRNPS 467
S +STPS ++ + S P L PS+ R + +PL P++SVE R+ S
Sbjct: 4 SDNSTPSRPRSITQPSLAFSSL---PPLSPSPSSSRRNSIPLMNPSASVESRDSS 55
>At5g41220.1 68418.m05009 glutathione S-transferase, putative
similar to emb|CAA10662
Length = 590
Score = 27.5 bits (58), Expect = 6.0
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +2
Query: 83 HTWNYSALHVHESVQSWRHCRHQRQWCS 166
+T NY + E +WR RH ++WCS
Sbjct: 370 YTNNYKSNFTLE--HAWRELRHSKKWCS 395
>At4g31490.1 68417.m04473 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 948
Score = 27.5 bits (58), Expect = 6.0
Identities = 12/39 (30%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +2
Query: 290 NIRVEHVKHSKCRQDFLKRVKENE-RLLKEAKAAGKTVN 403
N+ ++ + CRQ F+K + E + R ++E KA +T +
Sbjct: 628 NVEMKKIWLESCRQSFVKMISEKQLREMEELKAKTQTTH 666
>At3g45880.1 68416.m04965 hypothetical protein
Length = 431
Score = 27.5 bits (58), Expect = 6.0
Identities = 18/55 (32%), Positives = 24/55 (43%)
Frame = -2
Query: 418 WLSLQVDGLAGSLGFLQ*PLILFDSLKEVLSALGVLDMLNTDIDALRYNPSANTL 254
W +Q D FLQ L SL VLS D ++D + ++ PSA L
Sbjct: 299 WYDMQFDIKYAYFNFLQSLLYKSSSLNPVLSWREDEDSESSDAEGSKFTPSATNL 353
>At5g41240.1 68418.m05011 glutathione S-transferase, putative
similar to glutathione S-transferase, GST 10b
GB:CAA10662 [Arabidopsis thaliana] 37349.
Length = 591
Score = 27.1 bits (57), Expect = 7.9
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = +2
Query: 128 SWRHCRHQRQWCSS 169
+WR RH ++WCS+
Sbjct: 385 AWRELRHNKKWCST 398
>At1g76990.3 68414.m08966 ACT domain containing protein low
similarity to uridylyltransferase SP|P56884 from
Rhizobium meliloti; contains Pfam ACT domain PF01842
Length = 453
Score = 27.1 bits (57), Expect = 7.9
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 161 CSSKGYATQSIPWKDRSRVQRDCSCSRCDCQ 253
C KGY+ ++ +DR ++ D C+ D Q
Sbjct: 259 CEEKGYSVINVSCEDRPKLMFDIVCTLTDMQ 289
>At1g76990.2 68414.m08965 ACT domain containing protein low
similarity to uridylyltransferase SP|P56884 from
Rhizobium meliloti; contains Pfam ACT domain PF01842
Length = 453
Score = 27.1 bits (57), Expect = 7.9
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 161 CSSKGYATQSIPWKDRSRVQRDCSCSRCDCQ 253
C KGY+ ++ +DR ++ D C+ D Q
Sbjct: 259 CEEKGYSVINVSCEDRPKLMFDIVCTLTDMQ 289
>At1g76990.1 68414.m08964 ACT domain containing protein low
similarity to uridylyltransferase SP|P56884 from
Rhizobium meliloti; contains Pfam ACT domain PF01842
Length = 453
Score = 27.1 bits (57), Expect = 7.9
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 161 CSSKGYATQSIPWKDRSRVQRDCSCSRCDCQ 253
C KGY+ ++ +DR ++ D C+ D Q
Sbjct: 259 CEEKGYSVINVSCEDRPKLMFDIVCTLTDMQ 289
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,464,546
Number of Sequences: 28952
Number of extensions: 233283
Number of successful extensions: 719
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 719
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 987020800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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