BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20054 (677 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g33500.1 68414.m04146 hypothetical protein 32 0.30 At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 25 1.5 At4g23560.1 68417.m03394 glycosyl hydrolase family 9 protein sim... 29 2.1 At3g24740.1 68416.m03106 expressed protein 29 2.1 At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 2.8 At5g43680.1 68418.m05339 expressed protein 27 8.6 At5g01150.1 68418.m00019 hypothetical protein contains Pfam prof... 27 8.6 >At1g33500.1 68414.m04146 hypothetical protein Length = 254 Score = 32.3 bits (70), Expect = 0.30 Identities = 24/88 (27%), Positives = 45/88 (51%) Frame = +2 Query: 26 AIKHQIEKGVVDAITHEARYSLSEEKLLKEQIDYQTVTLHIVQDEFDEKVQCKVLDCDSI 205 +++ Q++ + + + ++LSE K LKE+ D +T T + EK Q K+ +S Sbjct: 26 SVEEQMQVTTISTLEKDLEHALSETKRLKEETDQKTRTRGEICSHILEK-QRKISSMESD 84 Query: 206 SQVKSKILDALFKNTPFHSDLLSMKLIS 289 S ++ L+ + + D LS KL+S Sbjct: 85 SVNIAQSLELILQ----ERDSLSAKLVS 108 >At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3) Similar to Arabidopsis luminal binding protein (gb|D89342); contains Pfam domain PF00012: dnaK protein Length = 678 Score = 25.4 bits (53), Expect(2) = 1.5 Identities = 10/36 (27%), Positives = 23/36 (63%) Frame = +2 Query: 98 EKLLKEQIDYQTVTLHIVQDEFDEKVQCKVLDCDSI 205 E +LKE +++ ++ ++++DEK++ L CD + Sbjct: 617 EGVLKEALEWLEENVNAEKEDYDEKLKEVELVCDPV 652 Score = 23.0 bits (47), Expect(2) = 1.5 Identities = 9/31 (29%), Positives = 19/31 (61%) Frame = +2 Query: 32 KHQIEKGVVDAITHEARYSLSEEKLLKEQID 124 K ++ + ++ + EA E+K++KE+ID Sbjct: 552 KGRLTEEEIEEMIREAEEFAEEDKIMKEKID 582 >At4g23560.1 68417.m03394 glycosyl hydrolase family 9 protein similar to cellulase GI:1039431 from [Phaseolus vulgaris] Length = 479 Score = 29.5 bits (63), Expect = 2.1 Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +3 Query: 504 YTVYNSDITDQNVHYYHLV-KPIEYQHIVNKSSEHSHKAIPEIFLTGCYL 650 Y+ YNSD + NVH +V P +K S++SH A P ++ ++ Sbjct: 420 YSYYNSDTPNPNVHIGAIVGGPNSSDQYSDKKSDYSH-AEPTTYINAAFI 468 >At3g24740.1 68416.m03106 expressed protein Length = 354 Score = 29.5 bits (63), Expect = 2.1 Identities = 15/41 (36%), Positives = 17/41 (41%) Frame = +1 Query: 295 RHGRGGHVTLQDEDVTTKTLNGWRKLNTLAHYGVKESAIMS 417 RH R H T + D WR+L YG SAI S Sbjct: 183 RHARRTHPTTRPSDTDPSRERAWRRLENQREYGDIVSAIRS 223 >At5g24530.1 68418.m02897 oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavanone 3-hydroxylase [Persea americana][GI:727410]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain Length = 341 Score = 29.1 bits (62), Expect = 2.8 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 2/49 (4%) Frame = +2 Query: 8 RFLLFKAIKHQIEKGVVDAITHEAR--YSLSEEKLLKEQIDYQTVTLHI 148 RF F+ I H + K ++D + AR +S+S E+ +K D T T + Sbjct: 61 RFGFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL 109 >At5g43680.1 68418.m05339 expressed protein Length = 232 Score = 27.5 bits (58), Expect = 8.6 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = +2 Query: 56 VDAITHEARYSLSEEKLLKEQIDYQ 130 VDAI+H AR+SL + L ++D Q Sbjct: 73 VDAISHGARFSLFDSSPLTRRVDNQ 97 >At5g01150.1 68418.m00019 hypothetical protein contains Pfam profile PF05056: Protein of unknown function (DUF674) Length = 501 Score = 27.5 bits (58), Expect = 8.6 Identities = 16/49 (32%), Positives = 26/49 (53%) Frame = +2 Query: 176 QCKVLDCDSISQVKSKILDALFKNTPFHSDLLSMKLISSGVMVEVDMLR 322 +C VLD +I+ + S L K H+D L +++IS +D+LR Sbjct: 414 KCTVLDDLTITSMNSCSTVCLLKKLQSHADDLEVQVISISNAEALDLLR 462 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,107,575 Number of Sequences: 28952 Number of extensions: 248384 Number of successful extensions: 610 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 599 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 610 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1428369392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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