Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20050
(647 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 179 2e-47
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 177 6e-47
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 175 2e-46
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 165 2e-43
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.002
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.1
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.5
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.5
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.4
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 179 bits (436), Expect = 2e-47
Identities = 90/119 (75%), Positives = 98/119 (82%)
Frame = +1
Query: 160 QTYHREVREGGPGNG*RILQICLGIGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGH 339
+ + +E +E G G+ + + + K ERGITIDIALWKFETSKYYVTIIDAPGH
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGH 95
Query: 340 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGETK 516
RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVG K
Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 141 bits (342), Expect = 5e-36
Identities = 65/66 (98%), Positives = 65/66 (98%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 91.9 bits (218), Expect = 5e-21
Identities = 41/42 (97%), Positives = 41/42 (97%)
Frame = +2
Query: 521 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 646
STEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG
Sbjct: 157 STEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 198
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 177 bits (432), Expect = 6e-47
Identities = 86/95 (90%), Positives = 88/95 (92%)
Frame = +1
Query: 232 IGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 411
+ + K ERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 3 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 62
Query: 412 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGETK 516
TGEFEAGISKNGQTREHALLAFTLGVKQLIVG K
Sbjct: 63 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 97
Score = 91.9 bits (218), Expect = 5e-21
Identities = 41/42 (97%), Positives = 41/42 (97%)
Frame = +2
Query: 521 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 646
STEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG
Sbjct: 100 STEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 141
Score = 23.4 bits (48), Expect = 1.9
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2
Query: 227 WVLDKLKAE 253
WVLDKLKAE
Sbjct: 1 WVLDKLKAE 9
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 175 bits (427), Expect = 2e-46
Identities = 88/119 (73%), Positives = 97/119 (81%)
Frame = +1
Query: 160 QTYHREVREGGPGNG*RILQICLGIGQTKG*AERGITIDIALWKFETSKYYVTIIDAPGH 339
+ + +E +E G G+ + + + K ERGITIDIALWKFET+KYYVTIIDAPGH
Sbjct: 40 EKFEKEAQEMGKGS----FKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGH 95
Query: 340 RDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGETK 516
RDFIKNMITGTSQADCAVLIVAAG GEFEAGISKNGQTREHALLAFTLGVKQLIVG K
Sbjct: 96 RDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 154
Score = 141 bits (342), Expect = 5e-36
Identities = 65/66 (98%), Positives = 65/66 (98%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 84.6 bits (200), Expect = 7e-19
Identities = 37/41 (90%), Positives = 39/41 (95%)
Frame = +2
Query: 524 TEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 646
T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPISGWHG
Sbjct: 158 TDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHG 198
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 165 bits (402), Expect = 2e-43
Identities = 79/81 (97%), Positives = 79/81 (97%)
Frame = +1
Query: 274 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 453
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 454 REHALLAFTLGVKQLIVGETK 516
REHALLAFTLGVKQLIVG K
Sbjct: 61 REHALLAFTLGVKQLIVGVNK 81
Score = 91.9 bits (218), Expect = 5e-21
Identities = 41/42 (97%), Positives = 41/42 (97%)
Frame = +2
Query: 521 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 646
STEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG
Sbjct: 84 STEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG 125
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.002
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +1
Query: 313 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 417
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.48
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 68 KTHINIVVIGHVDSGKST 121
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.1
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 77 INIVVIGHVDSGKST 121
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 2.5
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 69 FSLPIFG*SRITNCV*Y 19
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.5
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 298 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 399
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.4
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 298 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 399
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 183,989
Number of Sequences: 438
Number of extensions: 3602
Number of successful extensions: 23
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23
length of database: 146,343
effective HSP length: 55
effective length of database: 122,253
effective search space used: 19560480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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