BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20041 (743 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AY217747-1|AAP45005.1| 246|Apis mellifera short-chain dehydroge... 27 0.25 DQ058012-1|AAY57281.1| 373|Apis mellifera venom allergen acid p... 25 0.99 AY939855-1|AAX33235.1| 388|Apis mellifera venom acid phosphatas... 25 0.99 AF205594-1|AAQ13840.1| 156|Apis mellifera acid phosphatase prec... 23 2.3 AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr... 23 4.0 >AY217747-1|AAP45005.1| 246|Apis mellifera short-chain dehydrogenase/reductase protein. Length = 246 Score = 26.6 bits (56), Expect = 0.25 Identities = 9/41 (21%), Positives = 23/41 (56%) Frame = +1 Query: 139 VDQNKDSYKQLLKEAVAIPSVSCDVKYRADCIRMVHWMQTN 261 VD+ K ++L + + + CD+ + D ++++ W++ N Sbjct: 41 VDKMKTLVEELKSKPGKLVPLQCDLSNQNDILKVIEWVEKN 81 >DQ058012-1|AAY57281.1| 373|Apis mellifera venom allergen acid phosphatase protein. Length = 373 Score = 24.6 bits (51), Expect = 0.99 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +2 Query: 533 INAYKGTGAELPVNLKFIFECMEESGSEGLTAY*WTN*NL--RDSLIV*TMYVYPIT 697 + + G P + +I+ + S GLT WTN N+ R L T++ Y IT Sbjct: 160 LEEWTGKNITTPWDYYYIYHTLVAEQSYGLTLPSWTN-NIFPRGELFDATVFTYNIT 215 >AY939855-1|AAX33235.1| 388|Apis mellifera venom acid phosphatase precursor protein. Length = 388 Score = 24.6 bits (51), Expect = 0.99 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +2 Query: 533 INAYKGTGAELPVNLKFIFECMEESGSEGLTAY*WTN*NL--RDSLIV*TMYVYPIT 697 + + G P + +I+ + S GLT WTN N+ R L T++ Y IT Sbjct: 175 LEEWTGKNITTPWDYYYIYHTLVAEQSYGLTLPSWTN-NIFPRGELFDATVFTYNIT 230 >AF205594-1|AAQ13840.1| 156|Apis mellifera acid phosphatase precursor protein. Length = 156 Score = 23.4 bits (48), Expect = 2.3 Identities = 16/57 (28%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +2 Query: 533 INAYKGTGAELPVNLKFIFECMEESGSEGLTAY*WTN*NL--RDSLIV*TMYVYPIT 697 + + G P + +I+ + S GLT WTN N+ + L T++ Y IT Sbjct: 63 LEEWTGKNITTPWDYYYIYHTLVAEQSYGLTLPSWTN-NIFPKGELFDATVFTYNIT 118 >AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor protein. Length = 1370 Score = 22.6 bits (46), Expect = 4.0 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +3 Query: 45 FSCLSSTLFSEFQTSFCK 98 F CL+ST+ SE + CK Sbjct: 15 FLCLASTILSESAGTSCK 32 Score = 22.6 bits (46), Expect = 4.0 Identities = 17/78 (21%), Positives = 37/78 (47%), Gaps = 2/78 (2%) Frame = -2 Query: 703 TNSYRIYIHSLHYQRIPQVSICPLISCQAFGTRFLHTFENELQIDR--QLSTSALIGIDG 530 T+ + +++H RIP+ + PL+ + + TF + R +L+ + +G+ Sbjct: 92 TSLHELHVHGCKVLRIPEGAFQPLLELKKL---TVQTFNSVWGASRFLELAPDSFLGLRE 148 Query: 529 MQPTKYRPLSSVEPLPYN 476 + + S+V+ LP N Sbjct: 149 LHTLEIVE-SNVQALPVN 165 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 217,599 Number of Sequences: 438 Number of extensions: 4582 Number of successful extensions: 13 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 13 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 23266665 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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