Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20035
(493 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204; Euka... 102 4e-21
UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56; Euk... 100 2e-20
UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2; Ost... 92 5e-18
UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4; Bilateria|... 92 7e-18
UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3; Eukary... 91 2e-17
UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3; Eukary... 69 4e-11
UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1; Enceph... 53 4e-06
UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1; ... 52 5e-06
UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiel... 51 2e-05
UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole ge... 50 3e-05
UniRef50_Q9LPV3 Cluster: Putative 60S ribosomal protein L27a-1; ... 39 0.053
UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S riboso... 39 0.070
UniRef50_P53143 Cluster: Putative uncharacterized protein YGL102... 35 1.1
UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1; Flavobacteri... 34 2.0
UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse t... 33 3.5
UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella ve... 32 8.0
>UniRef50_P46776 Cluster: 60S ribosomal protein L27a; n=204;
Eukaryota|Rep: 60S ribosomal protein L27a - Homo sapiens
(Human)
Length = 148
Score = 102 bits (245), Expect = 4e-21
Identities = 43/78 (55%), Positives = 54/78 (69%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 201
M + +KTRKLRGHVS NAGG HHHRIN DKYHPGYFGK+GM++
Sbjct: 1 MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKH 60
Query: 202 FHFRKNKNFCPVLNLDKL 255
+H ++N++FCP +NLDKL
Sbjct: 61 YHLKRNQSFCPTVNLDKL 78
Score = 58.8 bits (136), Expect = 6e-08
Identities = 33/88 (37%), Positives = 42/88 (47%)
Frame = +3
Query: 201 FPL*KEQEFLSSFELR*AWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQ 380
+ L + Q F + L WTLVSEQTR+ A G P+I++V++ PKQ
Sbjct: 61 YHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQ 120
Query: 381 PVIVXXXXXXXXXXXXXXDVGGACVLSA 464
PVIV VGGACVL A
Sbjct: 121 PVIVKAKFFSRRAEEKIKSVGGACVLVA 148
>UniRef50_Q9LR33 Cluster: 60S ribosomal protein L27a-2; n=56;
Eukaryota|Rep: 60S ribosomal protein L27a-2 -
Arabidopsis thaliana (Mouse-ear cress)
Length = 146
Score = 100 bits (239), Expect = 2e-20
Identities = 46/78 (58%), Positives = 51/78 (65%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 201
MAT+ KK RK RGHVS NAGG HHHRI DKYHPGYFGK+GMR
Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60
Query: 202 FHFRKNKNFCPVLNLDKL 255
FH +NK FCP++NLDKL
Sbjct: 61 FHKLRNKFFCPIVNLDKL 78
>UniRef50_Q00V89 Cluster: 60s ribosomal protein L15/L27; n=2;
Ostreococcus|Rep: 60s ribosomal protein L15/L27 -
Ostreococcus tauri
Length = 198
Score = 92.3 bits (219), Expect = 5e-18
Identities = 41/76 (53%), Positives = 47/76 (61%)
Frame = +1
Query: 28 TSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRNFH 207
T K RK RGHVS AGG+HHHRI MDKYHPGYFGK+GMR+FH
Sbjct: 54 TQLHKNRKKRGHVSAGHGRIGKHRKHPAGRGMAGGQHHHRIMMDKYHPGYFGKVGMRHFH 113
Query: 208 FRKNKNFCPVLNLDKL 255
KN+ CP +N+DKL
Sbjct: 114 KLKNQTVCPTINIDKL 129
Score = 36.7 bits (81), Expect = 0.28
Identities = 23/84 (27%), Positives = 34/84 (40%)
Frame = +3
Query: 213 KEQEFLSSFELR*AWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIV 392
K Q + + WTLV + R + A + PVI++ P QPV+V
Sbjct: 116 KNQTVCPTINIDKLWTLVGTEKREECAKNTS-QAPVIDLTDHGIFKLLGKGQLPNQPVVV 174
Query: 393 XXXXXXXXXXXXXXDVGGACVLSA 464
+VGGAC+L+A
Sbjct: 175 KCRYISKLAEKKIKEVGGACILTA 198
>UniRef50_A1BQ56 Cluster: Ribosomal protein L27a; n=4;
Bilateria|Rep: Ribosomal protein L27a - Ascaris suum
(Pig roundworm) (Ascaris lumbricoides)
Length = 133
Score = 91.9 bits (218), Expect = 7e-18
Identities = 36/45 (80%), Positives = 40/45 (88%)
Frame = +1
Query: 121 NAGGEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVLNLDKL 255
NAGG+HHHRIN DKYHPGYFGK+GMRNFH RKN +CP +NLDKL
Sbjct: 20 NAGGQHHHRINFDKYHPGYFGKVGMRNFHVRKNGLYCPTVNLDKL 64
Score = 55.2 bits (127), Expect = 7e-07
Identities = 31/70 (44%), Positives = 36/70 (51%)
Frame = +3
Query: 255 WTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIVXXXXXXXXXXXXXX 434
W+LVSE+TR YA D K PVI++VKA PKQPVIV
Sbjct: 65 WSLVSEKTRTTYAEKKD-KAPVIDVVKAGYFKVLGKGLLPKQPVIVKAKFFSRTAEQKIK 123
Query: 435 DVGGACVLSA 464
VGGAC+L A
Sbjct: 124 GVGGACILVA 133
>UniRef50_Q00454 Cluster: 60S ribosomal protein L27a; n=3;
Eukaryota|Rep: 60S ribosomal protein L27a - Tetrahymena
thermophila
Length = 149
Score = 90.6 bits (215), Expect = 2e-17
Identities = 43/80 (53%), Positives = 50/80 (62%), Gaps = 2/80 (2%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXN--AGGEHHHRINMDKYHPGYFGKLGM 195
M + KKTRKLRGHVS AGG HHHRI M+K+HPGY+GKLGM
Sbjct: 1 MVSHLKKTRKLRGHVSHGHGRVGKHRKGGCRGGRGKAGGMHHHRILMEKWHPGYYGKLGM 60
Query: 196 RNFHFRKNKNFCPVLNLDKL 255
R FH +KN CPV+N+DKL
Sbjct: 61 RTFHLKKNPLHCPVVNIDKL 80
Score = 50.8 bits (116), Expect = 2e-05
Identities = 29/70 (41%), Positives = 35/70 (50%)
Frame = +3
Query: 255 WTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPKQPVIVXXXXXXXXXXXXXX 434
W+LVS+ TR KYA KVPVI++ KA P QPV+V
Sbjct: 81 WSLVSDATRQKYAEDKK-KVPVIDVTKAGFFKVLGKGRLPNQPVVVKAKYFSKTAERRIV 139
Query: 435 DVGGACVLSA 464
VGGACVL+A
Sbjct: 140 AVGGACVLTA 149
>UniRef50_Q9AW02 Cluster: 60S ribosomal protein L27A; n=3;
Eukaryota|Rep: 60S ribosomal protein L27A - Guillardia
theta (Cryptomonas phi)
Length = 144
Score = 69.3 bits (162), Expect = 4e-11
Identities = 32/78 (41%), Positives = 43/78 (55%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 201
M T +++RK RGHVS N+GG+HHH+I +KYH +FGK GMR
Sbjct: 1 MPTDIRRSRKKRGHVSSGHGRVGKHRKHPGGRGNSGGQHHHKIFFEKYHKKHFGKTGMRY 60
Query: 202 FHFRKNKNFCPVLNLDKL 255
+ K NF +NLDK+
Sbjct: 61 YRKLKQNNFNKSINLDKI 78
>UniRef50_O62581 Cluster: 60S ribosomal protein L27a; n=1;
Encephalitozoon cuniculi|Rep: 60S ribosomal protein L27a
- Encephalitozoon cuniculi
Length = 147
Score = 52.8 bits (121), Expect = 4e-06
Identities = 27/78 (34%), Positives = 38/78 (48%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 201
M KKTRKLRGHVS AGG H + ++HP Y GK GMR
Sbjct: 1 MTDRVKKTRKLRGHVSHGYGRVGKHRKHSGGRGLAGGFSHMKTFFTRFHPDYHGKRGMRV 60
Query: 202 FHFRKNKNFCPVLNLDKL 255
+H ++N ++ ++ +L
Sbjct: 61 YHRKENSDYARPISSARL 78
>UniRef50_Q4P992 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 187
Score = 52.4 bits (120), Expect = 5e-06
Identities = 25/50 (50%), Positives = 26/50 (52%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHP 171
M T KTRK RGHVS AGG+HHHR NMDKYHP
Sbjct: 1 MPTHLSKTRKHRGHVSAGNGRVGKHRKHPGGRGLAGGQHHHRTNMDKYHP 50
Score = 46.8 bits (106), Expect = 3e-04
Identities = 19/42 (45%), Positives = 27/42 (64%)
Frame = +1
Query: 130 GEHHHRINMDKYHPGYFGKLGMRNFHFRKNKNFCPVLNLDKL 255
G ++ + YFGK+GMR+FH +N +F PV+NLDKL
Sbjct: 77 GSQEMEVDREIGRDSYFGKVGMRHFHLTRNSSFRPVVNLDKL 118
>UniRef50_Q3LW97 Cluster: Ribosomal protein L27a; n=1; Bigelowiella
natans|Rep: Ribosomal protein L27a - Bigelowiella natans
(Pedinomonas minutissima) (Chlorarachnion sp.(strain
CCMP 621))
Length = 151
Score = 50.8 bits (116), Expect = 2e-05
Identities = 24/68 (35%), Positives = 33/68 (48%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 201
M T KK+RK+RG S +GG+HHH+I ++Y YF K GMR
Sbjct: 1 MVTKLKKSRKMRGKNSSGHGRINKHRKNPGGKGKSGGQHHHKIIFERYRETYFRKTGMRI 60
Query: 202 FHFRKNKN 225
++ K N
Sbjct: 61 YNLEKTLN 68
>UniRef50_A7QSF4 Cluster: Chromosome chr12 scaffold_159, whole
genome shotgun sequence; n=3; Vitis vinifera|Rep:
Chromosome chr12 scaffold_159, whole genome shotgun
sequence - Vitis vinifera (Grape)
Length = 151
Score = 50.0 bits (114), Expect = 3e-05
Identities = 19/33 (57%), Positives = 27/33 (81%)
Frame = +1
Query: 157 DKYHPGYFGKLGMRNFHFRKNKNFCPVLNLDKL 255
DK+HPG FGK+GMR+FH + K CP++++DKL
Sbjct: 28 DKHHPGNFGKVGMRHFHKLRIKLHCPIVHIDKL 60
>UniRef50_Q9LPV3 Cluster: Putative 60S ribosomal protein L27a-1;
n=1; Arabidopsis thaliana|Rep: Putative 60S ribosomal
protein L27a-1 - Arabidopsis thaliana (Mouse-ear cress)
Length = 104
Score = 39.1 bits (87), Expect = 0.053
Identities = 30/80 (37%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGK--LGM 195
M TS+KKTR LR HVS H R + PG G +GM
Sbjct: 1 MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39
Query: 196 RNFHFRKNKNFCPVLNLDKL 255
R FH +NK +C ++NLDKL
Sbjct: 40 RYFHKLRNKFYCQIVNLDKL 59
>UniRef50_UPI0000DA3FAD Cluster: PREDICTED: similar to 60S ribosomal
protein L27a; n=1; Rattus norvegicus|Rep: PREDICTED:
similar to 60S ribosomal protein L27a - Rattus
norvegicus
Length = 86
Score = 38.7 bits (86), Expect = 0.070
Identities = 21/51 (41%), Positives = 24/51 (47%)
Frame = +1
Query: 22 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPG 174
M+ +KTRK +GHVS NAGGEH HRIN PG
Sbjct: 1 MSGRLRKTRKFQGHVSDSHSRISKHHKHRGGQRNAGGEHLHRINCYYKVPG 51
>UniRef50_P53143 Cluster: Putative uncharacterized protein YGL102C;
n=2; Saccharomycetaceae|Rep: Putative uncharacterized
protein YGL102C - Saccharomyces cerevisiae (Baker's
yeast)
Length = 142
Score = 34.7 bits (76), Expect = 1.1
Identities = 18/57 (31%), Positives = 28/57 (49%)
Frame = -2
Query: 243 VQNWTEILVLSKVEISHTKFAKVSRMVLVHVDXXXXXXXXXXXXXXXXXVLSDTTMS 73
VQ+W + L VE+SHT +++ MV +HV+ VL+DTT +
Sbjct: 79 VQDWLPEMSLLLVEVSHTNLTEITWMVFIHVNSVVMLTTGHTSTTGVLSVLTDTTFT 135
>UniRef50_A4ANL3 Cluster: Putative lipoprotein; n=1;
Flavobacteriales bacterium HTCC2170|Rep: Putative
lipoprotein - Flavobacteriales bacterium HTCC2170
Length = 535
Score = 33.9 bits (74), Expect = 2.0
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = -1
Query: 454 TQAPPTSLIFFSADFEKNFAFTMTGCLGSLPL 359
T A SL FFS D + N A T GCLG+ P+
Sbjct: 306 TDAEGNSLYFFSLDTKDNSACTSAGCLGAWPV 337
>UniRef50_Q9ZQM0 Cluster: Putative non-LTR retroelement reverse
transcriptase; n=1; Arabidopsis thaliana|Rep: Putative
non-LTR retroelement reverse transcriptase - Arabidopsis
thaliana (Mouse-ear cress)
Length = 1044
Score = 33.1 bits (72), Expect = 3.5
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +3
Query: 156 GQVPSWILWQTWYEKFPL*KEQEFLSSFEL 245
GQ+P WILW W + L EQ+ +SS +L
Sbjct: 842 GQLPIWILWNLWNCRNKLIFEQKHISSMDL 871
>UniRef50_A7RPS0 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 306
Score = 31.9 bits (69), Expect = 8.0
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = -1
Query: 229 RNSCSF*SGNFSYQVCQSIQDGTCPC*FCDGAHHQHYHDLLDAYGA 92
R+SC + F +C+S T PC D H +H DL+D GA
Sbjct: 108 RSSCQ--ARCFMNNLCRSYNYNTTPCQLSDSDHLEHPSDLVDKPGA 151
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 459,080,676
Number of Sequences: 1657284
Number of extensions: 8617124
Number of successful extensions: 20201
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 19487
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20182
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 28437262108
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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