BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20027 (779 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family... 34 0.092 At1g62480.1 68414.m07050 vacuolar calcium-binding protein-relate... 31 0.65 At1g06670.1 68414.m00707 DEIH-box RNA/DNA helicase identical to ... 31 0.86 At3g49990.1 68416.m05466 expressed protein 30 1.5 At5g22810.1 68418.m02667 GDSL-motif lipase, putative similar to ... 29 3.5 At3g50750.1 68416.m05554 brassinosteroid signalling positive reg... 29 3.5 At5g61610.1 68418.m07731 glycine-rich protein / oleosin similar ... 29 4.6 At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 4.6 At1g20530.1 68414.m02558 hypothetical protein 29 4.6 At5g03380.1 68418.m00291 heavy-metal-associated domain-containin... 28 6.0 At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 28 6.0 At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 28 6.0 At1g76710.2 68414.m08928 SET domain-containing protein (ASHH1) l... 28 6.0 At1g76710.1 68414.m08927 SET domain-containing protein (ASHH1) l... 28 6.0 At3g05900.1 68416.m00664 neurofilament protein-related similar t... 28 8.0 At2g22450.1 68415.m02662 riboflavin biosynthesis protein, putati... 28 8.0 At1g11700.1 68414.m01343 expressed protein contains Pfam profile... 28 8.0 >At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 370 Score = 34.3 bits (75), Expect = 0.092 Identities = 25/88 (28%), Positives = 39/88 (44%), Gaps = 2/88 (2%) Frame = +2 Query: 512 EISKEEKPSATDAEGSADSAAIIPNMVK-KIDLAPTVES-DAAAIPEIKTPEAADAPKLA 685 E SK E P + E A +P + K ++ P V +A +PEI+ PE P+L Sbjct: 171 ENSKPEVPKLMETE--KPEAPKVPEIPKPELPKLPEVPKLEAPKVPEIQKPELPKMPELP 228 Query: 686 DNPVDEDKPADISPDAPKAEAKSADDLR 769 P + P+ PK EA +++ Sbjct: 229 KMPEIQKPELPKLPEVPKLEAPKVPEIQ 256 >At1g62480.1 68414.m07050 vacuolar calcium-binding protein-related contains weak similarity to vacuolar calcium binding protein [Raphanus sativus] gi|9049359|dbj|BAA99394 Length = 152 Score = 31.5 bits (68), Expect = 0.65 Identities = 22/86 (25%), Positives = 37/86 (43%), Gaps = 1/86 (1%) Frame = +3 Query: 255 EKKAEPAPVSNDEVPAIPEAKKDDIAPEDSDIAXXXXXXXXXXXXXXXXAKSSEIPDAEA 434 EK E + E P + E KKD+ AP ++ + K E+ + + Sbjct: 61 EKPIEETEEAQVETPEVVEIKKDEEAPVETPVV---VEDESKTEEVVEAKKEEEVEEKKT 117 Query: 435 KSADIKV-EEPAAQPEDSKTEVQATV 509 + A + V EE + E+ K V+A+V Sbjct: 118 EEAPVVVEEEKKPEAEEEKPAVEASV 143 >At1g06670.1 68414.m00707 DEIH-box RNA/DNA helicase identical to DEIH-box RNA/DNA helicase GB:BAA84364 GI:5881579 [Arabidopsis thaliana] Length = 1576 Score = 31.1 bits (67), Expect = 0.86 Identities = 23/92 (25%), Positives = 47/92 (51%), Gaps = 8/92 (8%) Frame = +2 Query: 521 KEEKPSATDAEGSADSAAIIPNMVK-----KIDLAPTVESDAAAIPEIK---TPEAADAP 676 K+E S + + ++ +I N+V +++AP ++ AA PE K + + Sbjct: 1306 KKEPKSVSKLDLGSEKVSIPSNLVNGNEQHDLNIAPGEDASAAKQPEKKRSRSKKRKSGN 1365 Query: 677 KLADNPVDEDKPADISPDAPKAEAKSADDLRL 772 L +++ KP+D++ + + E KSA++L L Sbjct: 1366 NLDLGKMEKSKPSDLANENEQTEPKSANNLDL 1397 >At3g49990.1 68416.m05466 expressed protein Length = 502 Score = 30.3 bits (65), Expect = 1.5 Identities = 14/41 (34%), Positives = 23/41 (56%) Frame = +3 Query: 417 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVLKFQKKKNLV 539 +P A+ D+K E P A+P KT Q + + +++KN V Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEEKKERKNAV 462 >At5g22810.1 68418.m02667 GDSL-motif lipase, putative similar to EXL3 (GP:15054386) [Arabidopsis thaliana] Length = 337 Score = 29.1 bits (62), Expect = 3.5 Identities = 16/47 (34%), Positives = 24/47 (51%) Frame = -1 Query: 326 IVLFGLWYSGYLVVTNWSRFGFLLSVAAATSVGLTESSILGATSRIG 186 +V+F ++ Y + T S FGF + A GL E+SIL +G Sbjct: 254 LVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSVG 300 >At3g50750.1 68416.m05554 brassinosteroid signalling positive regulator-related contains similarity to BZR1 protein [Arabidopsis thaliana] gi|20270971|gb|AAM18490 Length = 276 Score = 29.1 bits (62), Expect = 3.5 Identities = 21/71 (29%), Positives = 35/71 (49%), Gaps = 4/71 (5%) Frame = +2 Query: 482 FKN*STSYRAEISKEEKPSATDAEGSADSAAIIP---NMVKKIDLAP-TVESDAAAIPEI 649 F++ SY+A S PS T + + S +IP N+ +LAP + + A P I Sbjct: 103 FQSPIPSYQASPSSSSYPSPTRFDPNQSSTYLIPYLQNLASSGNLAPLRISNSAPVTPPI 162 Query: 650 KTPEAADAPKL 682 +P ++ P+L Sbjct: 163 SSPRRSN-PRL 172 >At5g61610.1 68418.m07731 glycine-rich protein / oleosin similar to variable surface lipoprotein Vsp422-3 (GI:15384285) [Mycoplasma bovis]; similar to glycine-rich protein atGRP-6, Arabidopsis thaliana, PIR:T49893 Length = 225 Score = 28.7 bits (61), Expect = 4.6 Identities = 23/87 (26%), Positives = 34/87 (39%) Frame = +2 Query: 452 SGRTSCSA*RFKN*STSYRAEISKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDA 631 SG+ S K R ++ +++KPS D D + + + P + A Sbjct: 108 SGKQSGGDNPLKEDKPPERDKLPRKDKPSKEDNLLKGDKPVEEDKLPAEEEKPPQKDKPA 167 Query: 632 AAIPEIKTPEAADAPKLADNPVDEDKP 712 E P D P D PV+EDKP Sbjct: 168 ----EGHKPPQKDKPAEGDKPVEEDKP 190 >At2g45800.1 68415.m05696 LIM domain-containing protein similar to PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain protein PLIM1 [Nicotiana tabacum] GI:5932418; contains Pfam profile PF00412: LIM domain Length = 226 Score = 28.7 bits (61), Expect = 4.6 Identities = 11/17 (64%), Positives = 14/17 (82%) Frame = +3 Query: 288 DEVPAIPEAKKDDIAPE 338 D+ +IPEAK+DD APE Sbjct: 197 DDTASIPEAKEDDAAPE 213 >At1g20530.1 68414.m02558 hypothetical protein Length = 614 Score = 28.7 bits (61), Expect = 4.6 Identities = 15/54 (27%), Positives = 24/54 (44%) Frame = +2 Query: 599 IDLAPTVESDAAAIPEIKTPEAADAPKLADNPVDEDKPADISPDAPKAEAKSAD 760 ++ P VES+A PE +PE+ + + D D P D + E + D Sbjct: 60 VESPPDVESEADESPETYSPESCSSAHSVTSS-DSDLPPKFDSDCEEGEGTNCD 112 >At5g03380.1 68418.m00291 heavy-metal-associated domain-containing protein similar to farnesylated protein ATFP2 [GI:4097545]; contains Pfam profile PF00403: Heavy-metal-associated domain Length = 392 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/84 (20%), Positives = 36/84 (42%) Frame = +2 Query: 512 EISKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAADAPKLADN 691 ++ + K + G+ D + + KI P A P+ +TP ++ + + Sbjct: 53 DVKIDYKSNKLTVIGNVDPVEVRDKVADKIK-RPVELVSTVAPPKKETPPSSGGAEKKPS 111 Query: 692 PVDEDKPADISPDAPKAEAKSADD 763 P E+KPA+ P A + + ++ Sbjct: 112 PAAEEKPAEKKPAAVEKPGEKKEE 135 >At2g43680.2 68415.m05430 calmodulin-binding family protein similar to SF16 protein [Helianthus annuus] GI:560150; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 669 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 5/49 (10%) Frame = +2 Query: 605 LAPTVESDAAAIPEIKTPEA----ADAPK-LADNPVDEDKPADISPDAP 736 L+P S A +P +P+ AD P+ L+ P D KP+ S +AP Sbjct: 127 LSPKPPSPRAEVPRSLSPKPPSPRADLPRSLSPKPFDRSKPSSASANAP 175 >At2g43680.1 68415.m05429 calmodulin-binding family protein similar to SF16 protein [Helianthus annuus] GI:560150; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 668 Score = 28.3 bits (60), Expect = 6.0 Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 5/49 (10%) Frame = +2 Query: 605 LAPTVESDAAAIPEIKTPEA----ADAPK-LADNPVDEDKPADISPDAP 736 L+P S A +P +P+ AD P+ L+ P D KP+ S +AP Sbjct: 126 LSPKPPSPRAEVPRSLSPKPPSPRADLPRSLSPKPFDRSKPSSASANAP 174 >At1g76710.2 68414.m08928 SET domain-containing protein (ASHH1) low similarity to huntingtin interacting protein 1 [Homo sapiens] GI:12697196; contains Pfam profile PF00856: SET domain; identical to cDNA ASH1-like protein 1 (ASHH1) partial cds GI:15488417 Length = 492 Score = 28.3 bits (60), Expect = 6.0 Identities = 16/65 (24%), Positives = 28/65 (43%) Frame = +2 Query: 518 SKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAADAPKLADNPV 697 + EEK E +S A+ N+ ++ D PT + +KT + D L+ N Sbjct: 276 TNEEKEKDISTENHLESTAL--NIQQQSDSTPTPMEEDVVTETVKTETSEDMKLLSQNSQ 333 Query: 698 DEDKP 712 ++ P Sbjct: 334 EDSSP 338 >At1g76710.1 68414.m08927 SET domain-containing protein (ASHH1) low similarity to huntingtin interacting protein 1 [Homo sapiens] GI:12697196; contains Pfam profile PF00856: SET domain; identical to cDNA ASH1-like protein 1 (ASHH1) partial cds GI:15488417 Length = 492 Score = 28.3 bits (60), Expect = 6.0 Identities = 16/65 (24%), Positives = 28/65 (43%) Frame = +2 Query: 518 SKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAADAPKLADNPV 697 + EEK E +S A+ N+ ++ D PT + +KT + D L+ N Sbjct: 276 TNEEKEKDISTENHLESTAL--NIQQQSDSTPTPMEEDVVTETVKTETSEDMKLLSQNSQ 333 Query: 698 DEDKP 712 ++ P Sbjct: 334 EDSSP 338 >At3g05900.1 68416.m00664 neurofilament protein-related similar to NF-180 (GI:632549) [Petromyzon marinus] similar to Neurofilament triplet H protein (200 kDa neurofilament protein) (Neurofilament heavy polypeptide) (NF-H) (Swiss-Prot:P12036) [Homo sapiens] Length = 673 Score = 27.9 bits (59), Expect = 8.0 Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 2/71 (2%) Frame = +2 Query: 518 SKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAA--DAPKLADN 691 SK+EK T GSA + I + +++ + P E++ A I E A + + D Sbjct: 302 SKDEK---TSESGSALCSEEILSTIQESNTDPIKETEGDASYPIDVIEKAITEEKHVVDE 358 Query: 692 PVDEDKPADIS 724 P +E+KP++ S Sbjct: 359 PANEEKPSESS 369 >At2g22450.1 68415.m02662 riboflavin biosynthesis protein, putative similar to SP|P50855 Riboflavin biosynthesis protein ribA [Includes: GTP cyclohydrolase II (EC 3.5.4.25); 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase)] {Actinobacillus pleuropneumoniae}; contains Pfam profiles PF00925: GTP cyclohydrolase II, PF00926: 3,4-dihydroxy-2-butanone 4-phosphate synthase Length = 476 Score = 27.9 bits (59), Expect = 8.0 Identities = 18/64 (28%), Positives = 28/64 (43%) Frame = +2 Query: 551 EGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAADAPKLADNPVDEDKPADISPD 730 EG S++ I+L P + S E+ P D+P++ D D D+P D Sbjct: 50 EGDVFSSSKSNGSSMGIELQPDLVSFGTLAAEM-IPTTMDSPEVEDEEFDLDRPTDGFAS 108 Query: 731 APKA 742 P+A Sbjct: 109 IPQA 112 >At1g11700.1 68414.m01343 expressed protein contains Pfam profile PF04520: Protein of unknown function, DUF584 Length = 201 Score = 27.9 bits (59), Expect = 8.0 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = +1 Query: 115 IVHSLRGCEFGDASGGGKGRIPAQ 186 IVH +RG G GGG GR+ Q Sbjct: 88 IVHQIRGGGEGGGGGGGGGRVERQ 111 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,507,245 Number of Sequences: 28952 Number of extensions: 244268 Number of successful extensions: 1001 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 952 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 999 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1746037600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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