BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20022 (392 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.08c |rpl35a|rpl33|60S ribosomal protein L35a|Schizosac... 73 2e-14 SPBC1921.01c |rpl3701|rpl37-1, rpl37|60S ribosomal protein L37|S... 71 9e-14 SPAC323.07c |||MatE family transporter|Schizosaccharomyces pombe... 25 3.2 SPAPB1A11.03 |||FMN dependent dehydrogenase|Schizosaccharomyces ... 25 4.2 SPAC16C9.03 |||export adaptor|Schizosaccharomyces pombe|chr 1|||... 25 5.5 SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 24 9.7 >SPCP31B10.08c |rpl35a|rpl33|60S ribosomal protein L35a|Schizosaccharomyces pombe|chr 3|||Manual Length = 108 Score = 72.5 bits (170), Expect = 2e-14 Identities = 43/112 (38%), Positives = 57/112 (50%) Frame = +2 Query: 2 GTRLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPG 181 G RLY KA ++R H T+++K+EG + +A FY GK YVY++ K Sbjct: 5 GHRLYVKAKHLSFQRSKHVIHPGTSIVKIEGCDSKEEAQFYLGKRVCYVYKSSKAV---- 60 Query: 182 GPRGKKNQAACYLGQGDPPTWQLWRVRAKFKSNLPAQAMGHRIRVMLYPSRI 337 RG K + G P VRA+F NLPA+ G +RVMLYPS I Sbjct: 61 --RGSKIRV--IWGTIARPHGNSGAVRARFVHNLPAKTFGSSLRVMLYPSNI 108 >SPBC1921.01c |rpl3701|rpl37-1, rpl37|60S ribosomal protein L37|Schizosaccharomyces pombe|chr 2|||Manual Length = 108 Score = 70.5 bits (165), Expect = 9e-14 Identities = 42/112 (37%), Positives = 58/112 (51%) Frame = +2 Query: 2 GTRLYAKAVFTGYKRGLRNQHENTALLKVEGAKDRNDAVFYAGKHCVYVYRAKKRTPIPG 181 G RLY KA ++R H T+L+K+EG + +A FY GK +VY++ K P+ Sbjct: 5 GHRLYVKAKHLSFQRSKHVIHPGTSLVKIEGCDSKEEAQFYLGKRICFVYKSNK--PV-- 60 Query: 182 GPRGKKNQAACYLGQGDPPTWQLWRVRAKFKSNLPAQAMGHRIRVMLYPSRI 337 RG K + G P VRA+F NLP + G +RVMLYPS + Sbjct: 61 --RGSKIRV--IWGTVSRPHGNSGVVRARFTHNLPPKTFGASLRVMLYPSNV 108 >SPAC323.07c |||MatE family transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 25.4 bits (53), Expect = 3.2 Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Frame = -2 Query: 226 LPQIARSLVFFATGTSGNWC-PLLSSV 149 LP +V+F TG G WC +LSSV Sbjct: 478 LPVTVFVVVYFNTGLKGIWCGMILSSV 504 >SPAPB1A11.03 |||FMN dependent dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 407 Score = 25.0 bits (52), Expect = 4.2 Identities = 15/40 (37%), Positives = 19/40 (47%) Frame = +3 Query: 183 VPVAKKTKLRAIWGKVTRPHGNSGVSEPSSSLTSLPRLWD 302 VP AKK + G V HG +SLT LP++ D Sbjct: 282 VPDAKKAVEYGMQGIVVSNHGGRQQDGGVASLTMLPKIVD 321 >SPAC16C9.03 |||export adaptor|Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 24.6 bits (51), Expect = 5.5 Identities = 14/47 (29%), Positives = 20/47 (42%) Frame = +3 Query: 63 TRTPLSSRLKEQKTVMMQSFMLASIASMCTELRRGHQFPEVPVAKKT 203 TRT L L TV+ + + + + R PEV + KKT Sbjct: 355 TRTHLGGILNAGNTVLAYHLAVTNFNNEVYDTLREDSIPEVVIVKKT 401 >SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 23.8 bits (49), Expect = 9.7 Identities = 11/25 (44%), Positives = 16/25 (64%), Gaps = 3/25 (12%) Frame = +2 Query: 80 LKVEGAKDRNDAVFYAGKH---CVY 145 +K + K + ++FYAGKH CVY Sbjct: 66 IKEKTEKTKLSSLFYAGKHEYFCVY 90 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,592,183 Number of Sequences: 5004 Number of extensions: 31464 Number of successful extensions: 78 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 74 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 74 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 130061696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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