BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS01000 (676 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 37 0.002 SPAPB17E12.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 35 0.009 SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 35 0.012 SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 34 0.016 SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 34 0.022 SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 31 0.15 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 31 0.15 SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 30 0.27 SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 30 0.27 SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizo... 30 0.35 SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosacch... 30 0.35 SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 30 0.35 SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pom... 28 1.4 SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 2.5 SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit L3|S... 26 4.3 SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3 |Schi... 26 4.3 SPAC17A5.13 |||GTP cyclohydrolase |Schizosaccharomyces pombe|chr... 26 4.3 SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 25 7.6 SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 25 10.0 SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 25 10.0 SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 25 10.0 SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 25 10.0 SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 25 10.0 >SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 37.1 bits (82), Expect = 0.002 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +3 Query: 171 EYDCAVCLQKCQHPTKLSCGHVFCFLCV 254 +++CA+C P +L C HVFC C+ Sbjct: 357 DFECAICSNVAYKPVRLGCSHVFCLHCL 384 >SPAPB17E12.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 311 Score = 35.1 bits (77), Expect = 0.009 Identities = 12/27 (44%), Positives = 19/27 (70%) Frame = +3 Query: 180 CAVCLQKCQHPTKLSCGHVFCFLCVRV 260 C +C +K ++P LS G VFC+ C++V Sbjct: 257 CKICGEKIKNPAVLSTGFVFCYPCIQV 283 >SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 269 Score = 34.7 bits (76), Expect = 0.012 Identities = 10/28 (35%), Positives = 17/28 (60%) Frame = +3 Query: 171 EYDCAVCLQKCQHPTKLSCGHVFCFLCV 254 +Y C +CL ++ + CGH+FC C+ Sbjct: 203 DYKCVICLDSPENLSCTPCGHIFCNFCI 230 >SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 34.3 bits (75), Expect = 0.016 Identities = 12/42 (28%), Positives = 22/42 (52%) Frame = +3 Query: 177 DCAVCLQKCQHPTKLSCGHVFCFLCVRV*LIKAENVQCAAQK 302 +C +C + Q P CGH +C+ C+ L ++++ QK Sbjct: 84 ECPICTEALQRPFTTHCGHTYCYECLLNWLKESKSCPTCRQK 125 >SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 306 Score = 33.9 bits (74), Expect = 0.022 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = +3 Query: 180 CAVCLQKCQHPTKLSCGHVFCFLCV 254 C++C++ P CGH+FC+ C+ Sbjct: 256 CSLCMEFIHCPAATECGHIFCWSCI 280 >SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 31.1 bits (67), Expect = 0.15 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +3 Query: 180 CAVCLQKCQHPTKLSCGHVFCFLCVR 257 C +C + + P SC H FC C+R Sbjct: 29 CLICHEYFRAPLITSCSHTFCSFCIR 54 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 31.1 bits (67), Expect = 0.15 Identities = 14/45 (31%), Positives = 20/45 (44%) Frame = +3 Query: 171 EYDCAVCLQKCQHPTKLSCGHVFCFLCVRV*LIKAENVQCAAQKF 305 E +C +C P CGH FC C+ L ++ QC +F Sbjct: 166 ELECQICFGMLYDPVVSPCGHTFCGPCLMQAL--TQSPQCPTCRF 208 >SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 30.3 bits (65), Expect = 0.27 Identities = 13/43 (30%), Positives = 19/43 (44%), Gaps = 6/43 (13%) Frame = +3 Query: 180 CAVCLQKCQH------PTKLSCGHVFCFLCVRV*LIKAENVQC 290 C +C+Q+ P CGH +C+ C+ L NV C Sbjct: 192 CMMCVQRGDERVAITTPYTTDCGHTYCYACIMSRLKLVNNVSC 234 >SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 30.3 bits (65), Expect = 0.27 Identities = 11/26 (42%), Positives = 17/26 (65%), Gaps = 1/26 (3%) Frame = +3 Query: 180 CAVCLQKCQHPTKLS-CGHVFCFLCV 254 C CL++ ++S CGHV+CF C+ Sbjct: 214 CPFCLEEKPVAARMSRCGHVYCFSCL 239 >SPCC330.01c |rhp16|SPCC613.13c, rad16|Rad16 homolog Rhp16|Schizosaccharomyces pombe|chr 3|||Manual Length = 963 Score = 29.9 bits (64), Expect = 0.35 Identities = 15/40 (37%), Positives = 19/40 (47%), Gaps = 3/40 (7%) Frame = +3 Query: 180 CAVCLQKCQHPTKLSCGHVFCFLCVRV*LIKA---ENVQC 290 C +C + Q + C H FC LCV + A ENV C Sbjct: 711 CKICDEVAQDAIESRCHHTFCRLCVTEYINAAGDGENVNC 750 >SPBC13E7.02 |cwf24||GCN5-related N acetyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 29.9 bits (64), Expect = 0.35 Identities = 13/45 (28%), Positives = 21/45 (46%), Gaps = 1/45 (2%) Frame = +3 Query: 165 NMEYDCAVCLQKCQHPTKLSCGHVFCFLCVRV*LIKAEN-VQCAA 296 ++ + C +C + + P +CGH FC C K +QC A Sbjct: 249 DIPFVCLICKKDYRSPIATTCGHHFCEQCAITRYRKTPTCIQCGA 293 >SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 897 Score = 29.9 bits (64), Expect = 0.35 Identities = 11/29 (37%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Frame = +3 Query: 171 EYDCAVCLQKCQHPT-KLSCGHVFCFLCV 254 +++C+VCL C P + CGH C C+ Sbjct: 603 DFNCSVCLDPCLAPVFIIPCGHFTCQECM 631 >SPBC713.07c |||vacuolar polyphosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 577 Score = 27.9 bits (59), Expect = 1.4 Identities = 12/37 (32%), Positives = 19/37 (51%) Frame = -3 Query: 533 SHCLEVNNDKVPASSKVHSDSPLLKAFSNSELYAHHI 423 S L N D V + D P++ A N+++Y H+I Sbjct: 147 SEILASNEDLVNKMIEAFPDVPIVSAIGNNDIYPHNI 183 >SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 268 Score = 27.1 bits (57), Expect = 2.5 Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +3 Query: 159 SHNMEYDCAVCLQKCQHPTKLS-CGH-VFCFLCV 254 S + DC +CLQK +LS CGH F + C+ Sbjct: 14 SRYLRRDCVICLQKDGLRAQLSPCGHDQFDYSCI 47 >SPBC3B9.14c |mrpl3||mitochondrial ribosomal protein subunit L3|Schizosaccharomyces pombe|chr 2|||Manual Length = 326 Score = 26.2 bits (55), Expect = 4.3 Identities = 13/38 (34%), Positives = 22/38 (57%) Frame = -3 Query: 575 LHPPPASMITSTELSHCLEVNNDKVPASSKVHSDSPLL 462 +H PP + S L EVN+ K+P +S+ ++ PL+ Sbjct: 286 IHSPPFRQLPSDYL----EVNDGKIPKASEPYTSIPLV 319 >SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 970 Score = 26.2 bits (55), Expect = 4.3 Identities = 12/28 (42%), Positives = 18/28 (64%), Gaps = 3/28 (10%) Frame = +2 Query: 269 QSRKCAMCRTEIPLDYFEN---PVLLDK 343 QSR C +CR+++P D N P+L +K Sbjct: 46 QSRACPLCRSKLPKDSRGNVLEPILSEK 73 >SPAC17A5.13 |||GTP cyclohydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 235 Score = 26.2 bits (55), Expect = 4.3 Identities = 6/23 (26%), Positives = 17/23 (73%) Frame = -2 Query: 234 HDRRIVLLDVDIFASKQHNHIPY 166 H+ +++ D+D+F+ +H+ +P+ Sbjct: 110 HEEMVIVRDIDVFSLCEHHLVPF 132 >SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosaccharomyces pombe|chr 3|||Manual Length = 680 Score = 25.4 bits (53), Expect = 7.6 Identities = 9/27 (33%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Frame = +3 Query: 180 CAVC-LQKCQHPTKLSCGHVFCFLCVR 257 C+VC ++ + CGH FC+ C++ Sbjct: 627 CSVCNFERWKDRIISLCGHGFCYQCIQ 653 >SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 504 Score = 25.0 bits (52), Expect = 10.0 Identities = 9/28 (32%), Positives = 16/28 (57%), Gaps = 3/28 (10%) Frame = +3 Query: 180 CAVCLQK---CQHPTKLSCGHVFCFLCV 254 C+ ++K C H T C + FC++C+ Sbjct: 294 CSTTIEKNGGCNHMTCKKCKYEFCWVCL 321 >SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 205 Score = 25.0 bits (52), Expect = 10.0 Identities = 9/25 (36%), Positives = 14/25 (56%) Frame = +3 Query: 225 CGHVFCFLCVRV*LIKAENVQCAAQ 299 CGH+FC C + ++ + V C Q Sbjct: 167 CGHLFCSTCAKE--LRKKTVPCPVQ 189 >SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 435 Score = 25.0 bits (52), Expect = 10.0 Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 5/45 (11%) Frame = +3 Query: 138 KHAVLIFSHNMEYDCAVCLQK-----CQHPTKLSCGHVFCFLCVR 257 K+A L+ ++ C VC + C T+ CGHV C C+R Sbjct: 160 KNAKLLEFQIRKFQCNVCFDEFNGTDCFQLTR--CGHVSCQSCLR 202 >SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1375 Score = 25.0 bits (52), Expect = 10.0 Identities = 7/25 (28%), Positives = 13/25 (52%) Frame = +3 Query: 180 CAVCLQKCQHPTKLSCGHVFCFLCV 254 C +C + +CGH++C C+ Sbjct: 1092 CIICRDIIKQGFITTCGHLYCSFCL 1116 >SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 677 Score = 25.0 bits (52), Expect = 10.0 Identities = 8/16 (50%), Positives = 11/16 (68%) Frame = +3 Query: 210 PTKLSCGHVFCFLCVR 257 P +L CGH+ F C+R Sbjct: 323 PKRLPCGHILHFHCLR 338 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,876,501 Number of Sequences: 5004 Number of extensions: 62326 Number of successful extensions: 164 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 309878492 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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