SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00999
         (679 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|...    69   8e-13
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    30   0.35 
SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces pomb...    29   0.47 
SPAC6F12.04 |||COPI-coated vesicle associated protein |Schizosac...    28   1.4  
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c...    27   3.3  
SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe...    27   3.3  
SPCC18.10 |||pyridoxine-pyridoxal-pyridoxamine kinase|Schizosacc...    26   4.4  
SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo...    26   5.8  
SPAC23G3.10c |ssr3||SWI/SNF and RSC complex subunit Ssr3|Schizos...    26   5.8  
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma...    26   5.8  
SPBC19F5.04 |||aspartate kinase |Schizosaccharomyces pombe|chr 2...    26   5.8  
SPBC30D10.16 |pha2||phrenate dehydratase|Schizosaccharomyces pom...    26   5.8  
SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase I|Schizosacchar...    25   7.6  

>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 537

 Score = 68.5 bits (160), Expect = 8e-13
 Identities = 33/101 (32%), Positives = 60/101 (59%)
 Frame = +1

Query: 250 KKDKTGKILTPAPFQGWLPSGTQARVEPNQKWFGNSRVISQNALQKFQEEFGAAVRNPYQ 429
           K +  G+++  A FQ       +AR++P+++WF N+RVI+Q  L +F+E  G  + +PYQ
Sbjct: 50  KYNAAGELVRAAEFQS--SEVPKARIQPDRRWFNNTRVIAQPTLTQFREAMGQKLNDPYQ 107

Query: 430 VIMKPTNLPITLLNEKAKNARVHLLDMKVLIGHLVLKSREK 552
           V+++   LP++LL E  +  +V +L+ +        KS+ K
Sbjct: 108 VLLRRNKLPMSLLQENTEIPKVRVLESEPFENTFGPKSQRK 148



 Score = 28.7 bits (61), Expect = 0.82
 Identities = 11/19 (57%), Positives = 14/19 (73%)
 Frame = +3

Query: 510 EGFDRTFGPKKQRKRVNLN 566
           E F+ TFGPK QRKR  ++
Sbjct: 135 EPFENTFGPKSQRKRPKIS 153


>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 230

 Score = 29.9 bits (64), Expect = 0.35
 Identities = 14/35 (40%), Positives = 17/35 (48%)
 Frame = -1

Query: 646 TLSISFSFIIFLRAFFYRFMQII*IAEFKFTLFLC 542
           + S SFSF+ FL   F  +     I  F F  FLC
Sbjct: 128 SFSFSFSFLFFLSQIFIVYFSSFPILHFLFFFFLC 162


>SPBC3F6.04c |||U3 snoRNP protein Nop14 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 827

 Score = 29.5 bits (63), Expect = 0.47
 Identities = 17/69 (24%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
 Frame = +2

Query: 68  IKSHTNMGKVRSTPGAPRKQGFNKSGHSMNPDR-PTEGLKGVGNPRSKGTIKRLQMYRNF 244
           ++SH  +G     P        +KS H ++P+R     L+       KG I+ L+    F
Sbjct: 734 LQSHRPLGITSQVPKFEEGYSLDKSSHDIDPERAQLNKLRAQHRDAKKGAIRTLRKDARF 793

Query: 245 KAKRIKPEK 271
            A+  + E+
Sbjct: 794 IARERRQEQ 802


>SPAC6F12.04 |||COPI-coated vesicle associated protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 132

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 15/33 (45%), Positives = 17/33 (51%)
 Frame = -1

Query: 634 SFSFIIFLRAFFYRFMQII*IAEFKFTLFLCFL 536
           SF F    R FFY FM  +  +   FT FL FL
Sbjct: 63  SFMFSFLGRGFFYAFMGTLLFSYSGFTSFLGFL 95


>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 386

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 9/22 (40%), Positives = 16/22 (72%)
 Frame = -1

Query: 574 IAEFKFTLFLCFLGPNVLSKPS 509
           + +F F++ L FLG N+L +P+
Sbjct: 166 LVDFSFSILLIFLGMNLLKEPA 187


>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 526

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 9/22 (40%), Positives = 16/22 (72%)
 Frame = +3

Query: 339 KMVWKFKSYITECIAKISRGVW 404
           +++W+ +  ITEC+AK   GV+
Sbjct: 378 RVLWERREGITECLAKAGSGVY 399


>SPCC18.10 |||pyridoxine-pyridoxal-pyridoxamine
           kinase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 340

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 10/21 (47%), Positives = 15/21 (71%), Gaps = 2/21 (9%)
 Frame = -3

Query: 362 TLEFPNHFWFGSTR--ACVPD 306
           T+ F NH  +G+TR  AC+P+
Sbjct: 47  TVHFSNHLGYGATRGSACIPE 67


>SPAC4F10.09c |||ribosome biogenesis protein Noc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 860

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 10/29 (34%), Positives = 20/29 (68%)
 Frame = -1

Query: 631 FSFIIFLRAFFYRFMQII*IAEFKFTLFL 545
           ++F  FL++F+++++QII    F   LF+
Sbjct: 180 WAFESFLKSFYFKYIQIIEALSFDALLFV 208


>SPAC23G3.10c |ssr3||SWI/SNF and RSC complex subunit
           Ssr3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 425

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +2

Query: 575 LNDLHKAVEESSEKYDETKRY*QSPCRF 658
           L +L +A+  S  KYD  K++ +SP  F
Sbjct: 335 LTELIQAITYSQSKYDFMKKFSESPIEF 362


>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 630

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 8/23 (34%), Positives = 14/23 (60%)
 Frame = -2

Query: 615 FSELSSTALCKSFRSLNLNSLFF 547
           F E+ +  LC +   +N+N LF+
Sbjct: 138 FKEIEACILCSALEKINVNELFY 160


>SPBC19F5.04 |||aspartate kinase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 519

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = +2

Query: 179 LKGVGNPRSKGTI 217
           +K VGNPR KGT+
Sbjct: 303 IKNVGNPRGKGTV 315


>SPBC30D10.16 |pha2||phrenate dehydratase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 287

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 10/21 (47%), Positives = 16/21 (76%)
 Frame = -2

Query: 273 NFSGFILFALKFRYICSLLIV 211
           +F+G ++FALKF  IC L ++
Sbjct: 34  SFAGKLIFALKFILICFLAVL 54


>SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase
           I|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 859

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 8/16 (50%), Positives = 12/16 (75%)
 Frame = -1

Query: 97  NFSHICMRFNTRLWKK 50
           N SH+C R N+++W K
Sbjct: 214 NISHLCTRKNSQVWGK 229


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,821,184
Number of Sequences: 5004
Number of extensions: 60931
Number of successful extensions: 162
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -