BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00996 (712 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g39740.1 68418.m04813 60S ribosomal protein L5 (RPL5B) riboso... 114 5e-26 At3g25520.1 68416.m03173 60S ribosomal protein L5 similar to 60S... 114 5e-26 At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NI... 27 9.3 At3g27025.1 68416.m03381 expressed protein 27 9.3 >At5g39740.1 68418.m04813 60S ribosomal protein L5 (RPL5B) ribosomal protein L5, rice Length = 301 Score = 114 bits (275), Expect = 5e-26 Identities = 50/77 (64%), Positives = 61/77 (79%) Frame = +1 Query: 1 GKTDYYARKRLVVQDKNKYNTPKYRLIVRLSNKDVTCQVAYSRIEGDHIVCAAYSHELPR 180 GKTDY AR RL+ QDKNKYNTPKYR +VR +NKD+ Q+ + I GD + +AY+HELP+ Sbjct: 26 GKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDIVAQIVSASIAGDIVKASAYAHELPQ 85 Query: 181 YGVKVGLTNYAAAYSTG 231 YG+ VGLTNYAAAY TG Sbjct: 86 YGLTVGLTNYAAAYCTG 102 Score = 85.4 bits (202), Expect = 3e-17 Identities = 39/66 (59%), Positives = 52/66 (78%) Frame = +3 Query: 309 EYNVEPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESK 488 +++VEP D+ FR LDVGL RTTTG RVFGA+KGA+DGGL++PHS KRF G+ E+K Sbjct: 129 DFSVEPTDSRR-PFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRFAGFHKENK 187 Query: 489 KFNAEV 506 + +AE+ Sbjct: 188 QLDAEI 193 Score = 73.7 bits (173), Expect = 1e-13 Identities = 32/62 (51%), Positives = 45/62 (72%) Frame = +2 Query: 509 RAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKK 688 R +I+G HV+ YM+ L +D+ + + FS YIK GV A++IE +YKK H AIRA+P+HKK Sbjct: 195 RNYIYGGHVSNYMKLLGEDEPEKLQTHFSAYIKKGVEAESIEEMYKKVHAAIRAEPNHKK 254 Query: 689 KE 694 E Sbjct: 255 TE 256 >At3g25520.1 68416.m03173 60S ribosomal protein L5 similar to 60S ribosomal protein L5 GB:P49625 from [Oryza sativa] Length = 301 Score = 114 bits (275), Expect = 5e-26 Identities = 50/77 (64%), Positives = 61/77 (79%) Frame = +1 Query: 1 GKTDYYARKRLVVQDKNKYNTPKYRLIVRLSNKDVTCQVAYSRIEGDHIVCAAYSHELPR 180 GKTDY AR RL+ QDKNKYNTPKYR +VR +NKD+ Q+ + I GD + +AY+HELP+ Sbjct: 26 GKTDYRARIRLINQDKNKYNTPKYRFVVRFTNKDIVAQIVSASIAGDIVKASAYAHELPQ 85 Query: 181 YGVKVGLTNYAAAYSTG 231 YG+ VGLTNYAAAY TG Sbjct: 86 YGLTVGLTNYAAAYCTG 102 Score = 85.4 bits (202), Expect = 3e-17 Identities = 39/66 (59%), Positives = 52/66 (78%) Frame = +3 Query: 309 EYNVEPVDNGPGAFRCYLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESK 488 +++VEP D+ FR LDVGL RTTTG RVFGA+KGA+DGGL++PHS KRF G+ E+K Sbjct: 129 DFSVEPTDSRR-PFRALLDVGLIRTTTGNRVFGALKGALDGGLDIPHSDKRFAGFHKENK 187 Query: 489 KFNAEV 506 + +AE+ Sbjct: 188 QLDAEI 193 Score = 70.1 bits (164), Expect = 1e-12 Identities = 31/60 (51%), Positives = 43/60 (71%) Frame = +2 Query: 509 RAHIFGLHVAEYMRSLEQDDEDSFKRQFSKYIKLGVTADAIEAIYKKAHEAIRADPSHKK 688 R +I+G HV+ YM+ L +D+ + + FS YIK GV A++IE +YKK H AIRADP+ KK Sbjct: 195 RNYIYGGHVSNYMKLLGEDEPEKLQTHFSAYIKKGVEAESIEELYKKVHAAIRADPNPKK 254 >At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NIFS) identical to Cysteine desulfurase, mitochondrial precursor (SP:O49543) {Arabidopsis thaliana}; identical to cDNA GI:12656131; contains Pfam profile PF00266: aminotransferase, class V Length = 453 Score = 27.5 bits (58), Expect = 9.3 Identities = 19/51 (37%), Positives = 24/51 (47%) Frame = +3 Query: 357 YLDVGLARTTTGARVFGAMKGAVDGGLNVPHSIKRFPGYDAESKKFNAEVQ 509 YLD+ A T RVF AM + PHS G++AE+ NA Q Sbjct: 56 YLDMQ-ATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQ 105 >At3g27025.1 68416.m03381 expressed protein Length = 299 Score = 27.5 bits (58), Expect = 9.3 Identities = 20/48 (41%), Positives = 26/48 (54%), Gaps = 8/48 (16%) Frame = +2 Query: 587 QFSKYIKLGVTADAIEAIYKKAHEA---IRADP-----SHKKKELKKD 706 Q YI+ T DAI+ ++KK H A R D S KKK+LKK+ Sbjct: 183 QGEPYIEKHSTRDAIKRVFKKLHGASSKTRNDDEDDSMSKKKKDLKKN 230 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,246,422 Number of Sequences: 28952 Number of extensions: 303642 Number of successful extensions: 798 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 764 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 798 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1535986264 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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