Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00986
(696 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 139 4e-34
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 131 8e-32
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 117 2e-27
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 111 7e-26
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 95 1e-20
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 51 1e-07
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 1e-04
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.36
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.84
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 3.4
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 5.9
SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 25 7.8
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 139 bits (336), Expect = 4e-34
Identities = 63/87 (72%), Positives = 74/87 (85%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXXGKPKIKVAYKGEDKTFFPEEVSSM 427
P +TIFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
VL KM+ETAEAYLG V +AV+TVPAY
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAY 147
Score = 113 bits (271), Expect = 3e-26
Identities = 54/62 (87%), Positives = 57/62 (91%)
Frame = +1
Query: 511 NDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 690
NDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGTFDVS+L
Sbjct: 149 NDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSLL 208
Query: 691 TI 696
TI
Sbjct: 209 TI 210
Score = 112 bits (270), Expect = 4e-26
Identities = 51/59 (86%), Positives = 56/59 (94%)
Frame = +3
Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 254
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPH 62
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 131 bits (317), Expect = 8e-32
Identities = 59/87 (67%), Positives = 72/87 (82%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXXGKPKIKVAYKGEDKTFFPEEVSSM 427
P +TIFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSM
Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
VL KM+E+AEA+LG V +AV+TVPAY
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAY 147
Score = 112 bits (270), Expect = 4e-26
Identities = 51/59 (86%), Positives = 56/59 (94%)
Frame = +3
Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 254
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPH 62
Score = 109 bits (263), Expect = 3e-25
Identities = 53/62 (85%), Positives = 56/62 (90%)
Frame = +1
Query: 511 NDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 690
NDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGTFDVS+L
Sbjct: 149 NDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSLL 208
Query: 691 TI 696
TI
Sbjct: 209 TI 210
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 117 bits (281), Expect = 2e-27
Identities = 53/87 (60%), Positives = 67/87 (77%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXXGKPKIKVAYKGEDKTFFPEEVSSM 427
P++T+FDAKRLIGR++ED Q D+KHWPF+V+ G P I+V Y GE K F +E+S+M
Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
VLTKMKE +EA L K V+ AVITVPAY
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAY 151
Score = 103 bits (246), Expect = 3e-23
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +1
Query: 511 NDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 690
+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGTFDVS+L
Sbjct: 153 SDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDVSLL 212
Query: 691 TI 696
I
Sbjct: 213 KI 214
Score = 100 bits (240), Expect = 2e-22
Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%)
Frame = +3
Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP-N 254
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67
Query: 255 TQY 263
T +
Sbjct: 68 TVF 70
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 111 bits (268), Expect = 7e-26
Identities = 49/87 (56%), Positives = 69/87 (79%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXXGKPKIKVAYKGEDKTFFPEEVSSM 427
P++TIFD KRLIGRKF++ T+ D+K +PF +V+ +P ++V G+ K F PEE+S+M
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
+L+KMK+TAEAYLGK V +AV+TVPAY
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAY 180
Score = 103 bits (247), Expect = 2e-23
Identities = 47/62 (75%), Positives = 59/62 (95%)
Frame = +1
Query: 511 NDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 690
ND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T E++++++DLGGGTFDVS+L
Sbjct: 182 NDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGTFDVSLL 240
Query: 691 TI 696
+I
Sbjct: 241 SI 242
Score = 98.7 bits (235), Expect = 7e-22
Identities = 45/64 (70%), Positives = 54/64 (84%), Gaps = 1/64 (1%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP-NT 257
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 258 QYSM 269
+ +
Sbjct: 98 IFDI 101
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 94.7 bits (225), Expect = 1e-20
Identities = 45/62 (72%), Positives = 53/62 (85%)
Frame = +1
Query: 511 NDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSIL 690
NDSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGTFD+SIL
Sbjct: 195 NDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGTFDISIL 252
Query: 691 TI 696
+
Sbjct: 253 EL 254
Score = 82.6 bits (195), Expect = 5e-17
Identities = 38/87 (43%), Positives = 56/87 (64%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXXGKPKIKVAYKGEDKTFFPEEVSSM 427
P++T F KRLIGR+F++ VQ D+K P+++V + KT+ P ++
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
+L+KM+ETA YLGK V+NAV+TVPAY
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAY 193
Score = 73.7 bits (173), Expect = 2e-14
Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 1/66 (1%)
Frame = +3
Query: 69 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 245
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 246 NPNTQY 263
NP +
Sbjct: 109 NPENTF 114
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 51.2 bits (117), Expect = 1e-07
Identities = 21/61 (34%), Positives = 39/61 (63%)
Frame = +3
Query: 66 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 245
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62
Query: 246 N 248
N
Sbjct: 63 N 63
Score = 49.2 bits (112), Expect = 6e-07
Identities = 27/63 (42%), Positives = 37/63 (58%), Gaps = 4/63 (6%)
Frame = +1
Query: 514 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGGTFDV 681
D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G + V
Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNYSV 213
Query: 682 SIL 690
SI+
Sbjct: 214 SIV 216
Score = 41.9 bits (94), Expect = 8e-05
Identities = 25/87 (28%), Positives = 45/87 (51%), Gaps = 1/87 (1%)
Frame = +2
Query: 251 QHTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSXXGKPKIKVAYKGEDKTFFPEEVSSM 427
++T+ KRL GR ++D ++ + ++ G KV Y E+ F ++ +
Sbjct: 65 RNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAA 124
Query: 428 VLTKMKETAEAYLGKTVQNAVITVPAY 508
TK+K AEA L +V + VI++PA+
Sbjct: 125 YFTKIKAIAEAELIGSVSDVVISIPAW 151
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 41.5 bits (93), Expect = 1e-04
Identities = 17/56 (30%), Positives = 31/56 (55%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 248
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRN 82
Score = 31.1 bits (67), Expect = 0.16
Identities = 21/91 (23%), Positives = 44/91 (48%), Gaps = 6/91 (6%)
Frame = +2
Query: 254 HTIFDAKRLIGRKFEDATVQADMKHWPFEVVSXXGKPKIKVAYKGED------KTFFPEE 415
+++ + + L+G+ ++ T+ H+ V+ G+ K+ + ++ K E
Sbjct: 85 NSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDEESDPKEKILTAHE 142
Query: 416 VSSMVLTKMKETAEAYLGKTVQNAVITVPAY 508
S L ++ E+AE +LG V V++VP Y
Sbjct: 143 ASVRHLRRLTESAEDFLGTKVNGCVMSVPVY 173
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 29.9 bits (64), Expect = 0.36
Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNT 257
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82
Query: 258 QYSMPNV 278
+S+ NV
Sbjct: 83 AHSIRNV 89
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.84
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 73 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 168
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +3
Query: 177 PSYVAFTDTERLIGDAAKNQVAMNPNTQYSMPNVSSDVSSKMLLC 311
P +A T ER++ AAK A+N Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.8 bits (54), Expect = 5.9
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = -1
Query: 159 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 1
G W SP S + H ++++ +F +RVL C+ L K +K+A L
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323
>SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 675
Score = 25.4 bits (53), Expect = 7.8
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +3
Query: 222 AAKNQVAMNPNTQYSMPNVSSDVSSKML 305
A K Q+ +NP + +PNV D + +L
Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,962,477
Number of Sequences: 5004
Number of extensions: 62438
Number of successful extensions: 209
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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